Abstract:
:Metastasis is the main cause of death in osteosarcoma. Targeting the process of metastasis is a main strategy for osteosarcoma therapy. As a traditional Chinese medicine, Zanthoxylum nitidum (Roxb) has been applied to treat various diseases, including cancer. However, no evidence has been shown on the anti-metastasis effect of nitidine chloride (NC) that was extracted from Zanthoxylum nitidum (Roxb) on osteosarcoma cells, or its underling mechanisms. In the present study, we aimed to demonstrate the role of NC on the migration and invasion of osteosarcoma cells. Viability and proliferation of osteosarcoma cells were examined by MTT assay. Then, by appling scratch wound healing assay and Transwell assays, we evaluated migratory and invasive ability of the cells, respectively. Moreover, the expression of epithelial-to-mesenchymal transition (EMT) markers were determined after treatment with NC. Furthermore, the expression of Akt, GSK-3β and Snail were detected by western blot analysis. In addition, the GSK-3β activity was examined by GSK-3β kinase assay. Finally, an inhibitor of GSK-3β, lithium chloride (LiCl) was applied to testify the effect of NC on the expression of EMT markers and Snail. We found that the proliferative, migratory and invasive ability of the U2OS osteosarcoma cells were all suppressed when treated with NC. NC increased the expression of E-cadherin and decreased the expression of N-cadherin, vimentin and fibronectin in a dose-dependent manner. NC also exerted its ability to suppress the phosphorylation of Akt and GSK-3β so as to activate GSK-3β. Then, by using an GSK-3β inhibitor, LiCl, we revealed the effect of GSK-3β in the expression of EMT markers. The expression of Snail was inhibited when treated with NC and LiCl also reversed the NC-inhibited Snail expression. Taken together, these results revealed that NC suppressed EMT and decreased the invasive ability of osteosarcoma cells via the Akt/GSK-3β/snail signaling pathway.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Cheng Z,Guo Y,Yang Y,Kan J,Dai S,Helian M,Li B,Xu J,Liu Cdoi
10.3892/or.2016.4846subject
Has Abstractpub_date
2016-08-01 00:00:00pages
1023-9issue
2eissn
1021-335Xissn
1791-2431journal_volume
36pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Recent studies have shown that miRNAs have potent abilities to activate gene expression by targeting promoter elements, a phenomenon known as RNA activation (RNAa). In the present study, we identified a new endogenous miR-3619-5p which was decreased in prostate cancer tissues and cells compared to corresponding normal...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5250
更新日期:2017-01-01 00:00:00
abstract::The development and progression of epithelial cancers are the result of an imbalance in signals promoting and inhibiting cellular proliferation and apoptosis. The aim of this study is to evaluate the expression of cell-cycle and apoptosis regulators and correlate them with clinical outcome in the most frequent carcino...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.14.6.1527
更新日期:2005-12-01 00:00:00
abstract::External abdomino-pelvic irradiation after primary surgery in early stages of epithelial ovarian carcinoma has been used as adjuvant therapy. The aims of this study were to evaluate efficacy and tolerability of abdomino-pelvic radiotherapy in ovarian carcinomas and to find predictive factors for recurrent disease. Fro...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::Epigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5008
更新日期:2016-10-01 00:00:00
abstract::We investigated whether 18F-fluorothymidine-positron-emission tomography/computed tomography (18F-FLT-PET/CT) is useful for the evaluation of the very early response to anti-epidermal growth factor receptor (EGFR) antibody cetuximab therapy in human lung cancer xenografts. A human tumor xenograft model was established...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1338
更新日期:2011-09-01 00:00:00
abstract::Studies of tumor-infiltrating immune cells have revealed that immune escape plays an important role in tumor growth. The aim of the present study was to investigate the impact of metastasis affecting CD4+ T cell subsets in human clinical samples. Single-cell suspensions derived from tumor-draining lymph node (TDLN) an...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4064
更新日期:2015-08-01 00:00:00
abstract::MicroRNAs (miRNAs) play an important role in the genesis and development of gastric cancer. In the present study, we determined whether miRNA-135a-5p expression was increased in gastric cancer compared with adjacent non-tumor tissues using 20 pairs of gastric cancer and para-carcinoma tissue samples which were assesse...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3546
更新日期:2014-12-01 00:00:00
abstract::Resistance to chemotherapeutic agents is a major problem for successful cancer treatment. P-glycoprotein (Pgp), a product of the multidrug resistance (MDR)1 gene expressed in cancer cells, is one of the mechanism of MDR. However, there are few reports regarding the effects of Pgp on prognosis of colorectal cancer (CRC...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.4.815
更新日期:2001-07-01 00:00:00
abstract::Loss of proliferative control and failure to undergo cellular differentiation are key events during carcinogenesis. We recently identified a new potential tumor suppressor gene named MTUS1 (mitochondrial tumor suppressor 1), down-regulated in undifferentiated tumor cell lines, inhibiting tumor cell proliferation after...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::Cyclin Y (CCNY) is a newly identified PFTK1 interacting protein and has been found to be associated with the proliferation and tumorigenesis of human non-small cell lung cancer. In the present study, we analyzed the expression levels of CCNY in 65 cases of breast cancer (BC) tissues and in four BC cell lines, BT-474, ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5126
更新日期:2016-11-01 00:00:00
abstract::In the development and progression of cancer, tumor suppressor genes may be silenced by mechanisms such as methylation. Thus the discovery of new genes silenced by methylation may uncover new tumor suppressor genes, and improve our understanding of cancer biology. In this study we investigated the methylation of 19 ge...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000325
更新日期:2009-04-01 00:00:00
abstract::It has been demonstrated that homozygous mutations at the L(2)gl locus in Drosophila result in the development of tumor in the presumptive adult optic centers of the larval brain and of the imaginal discs. We previously cloned an L(2)gl homologue, Rgl-1, in the rat brain. In this study, we analyzed the capability of R...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
abstract::Minimal residual disease (MRD) is derived from tumor-initiating cells (TICs) and is responsible for tumor relapse. Neuroblastoma is characterized by extreme tumor heterogeneity, and more than half of high-risk patients experience tumor relapse. To overcome tumor heterogeneity and ac...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2286
更新日期:2013-04-01 00:00:00
abstract::Berbamine is an herbal compound derived from Berberis amurensis, which is used in Chinese traditional medicine. However, few studies have investigated this anti-tumor effect or the underlying mechanisms of berbamine on lymphoma cells. We investigate the effect, as well as the mechanism of action, of 4-chlorobenzoyl be...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000688
更新日期:2010-03-01 00:00:00
abstract::Cumulative evidence suggests that long non-coding RNAs (lncRNAs) may be good biomarkers in various types of tumors. In the present study, we mined lncRNA expression profiling in 739 lung cancer patients from Gene Expression Omnibus (GEO) datasets. A risk score model was constructed based on the expression data of thes...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4817
更新日期:2016-07-01 00:00:00
abstract::The antitumor effect of photodynamic therapy (PDT) mediated by a novel photosensitizer I (Ps I; {γ-[N-poly(ethyleneglycol)]folic acid}-5,10,15-tris(3-hydroxyphenyl)-20-(4-carboxyphenyl)chlorin), in which chlorin was used as a photoactive unit, folic acid as a tumor‑targeting warhead, and polyethylene glycol as a linke...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3593
更新日期:2015-01-01 00:00:00
abstract::The restricted expression of Wilms tumor 1 (WT1) and cyclin A1 (CCNA1) in normal tissues, as opposed to their abnormal expression in leukemia demonstrates the applicability of WT1 and CCNA1 as cancer antigens for immunotherapy, and as markers for prognosis and relapse. In this study, the WT1 and CCNA1 mRNA levels were...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7287
更新日期:2019-11-01 00:00:00
abstract::Histone deacetylases (HDACs) have been shown to play important roles in the regulation of chromatin remodeling by histone deacetylation, and their expression is induced in several types of cancer. In addition, they are known to be associated with resistance to anticancer drugs. However, the relevance of HDAC4 in chemo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2578
更新日期:2013-09-01 00:00:00
abstract::Gastric cancer is currently the fourth most common cancer and the third leading cause of cancer‑associated mortality worldwide. Studies have identified that certain biomarkers contribute to the prognosis, diagnosis and treatment of gastric cancer. However, the biomarkers of gastric cancer are rarely used clinically. T...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6794
更新日期:2019-01-01 00:00:00
abstract::Epigenetic mechanisms of histone acetylation/deacetylation play an important role in the regulation of gene expression associated with the cell cycle and apoptosis. Recently, sodium butyrate, a histone deacetylase (HDAC) inhibitor, has been shown to exhibit anticancer effects via di...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1585
更新日期:2012-04-01 00:00:00
abstract::Caspases play an essential role in the initiation/regulation of apoptosis. Aberrant apoptotic regulation has been associated with carcinogenesis and therapeutic resistance. To explore the possible involvement of altered caspase expression in breast cancer, we have systematically examined the expression of both protein...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::Neuroblastoma is the most common inheritable, solid neoplasm in children found under the age of 7 and accounts for approximately 7% of childhood cancers. A common treatment that has been prescribed for over a decade is retinoid therapy [using all‑trans retinoic acid (RA)]. Treatment with this differentiating agent has...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7681
更新日期:2020-09-01 00:00:00
abstract::Chronic myelogenous leukemia (CML) has a typical progressive course with transition from a chronic phase to a terminal blast crisis phase. However, the mechanisms that lead to disease progression remain unclear. Bone marrow mesenchymal stem cells (BMMSCs) play important roles in maintaining the bone marrow microenviro...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2518
更新日期:2013-08-01 00:00:00
abstract::Casticin, a polymethoxyflavone, has been reported to exert anticancer activities. The objectives of this study were to examine the molecular mechanisms by which casticin induces the growth inhibition and cell cycle arrest in human hepatocellular carcinoma (HCC) cells. The HCC cell l...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2076
更新日期:2013-01-01 00:00:00
abstract::This study analyzed microRNA (miRNA) and mRNA expression profiles and investigated the biological characteristics of ESCC by using invasion and cytotoxicity cell models. miRNA profiles were evaluated through miRNA microarray. Transwell chamber and nedaplatin (NDP) were used to construct invasion and cytotoxicity cell ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5776
更新日期:2017-08-01 00:00:00
abstract::Human telomerase reverse transcriptase (hTERT) is an attractive target for cancer gene therapy. However, the poor inhibition of telomerase and a time lag between the inhibition and arrest of cell proliferation limits its use in cancer gene therapy. RNA interference (RNAi) has been proposed as a potential technique for...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-02-01 00:00:00
abstract::Human colon cancer cells were implanted subcutaneously into nude mice. After 12 days, the animals were divided into two groups. The first group received 40 microg/kg body weight of octreotide, galanin and serotonin via an intraperitoneally implanted pump. The second group received sterile saline only. Treatment lasted...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::In a human cancer cell line, we previously found a mutation in codon 322 of the extracellular signal-regulated kinase (ERK2E322K), the protein showed a faster migration when compared to wild-type in SDS-PAGE and constitutive phosphorylation. However, the reason for the faster migration, and the biochemical and biologi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-10-01 00:00:00
abstract::MicroRNAs (miRNAs) that negatively regulate gene expression play a key role in the development and progression of cancer. Aberrant expression of hsa-miR-187 (miR-187) has been reported in various malignancies. However, the function of miR-187 in tumor progression remains controversial and its role in non-small cell lu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5548
更新日期:2017-05-01 00:00:00
abstract::Breast cancer which is the most common type of diagnosed cancer among women worldwide possesses metastatic potential, multi‑drug resistance, and high mortality. The NF‑κB signaling pathway has been revealed to be abnormally activated in breast cancer cells and closely associated with high metastasis and poor prognosis...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7891
更新日期:2020-12-09 00:00:00