3D-reconstruction and functional properties of GFP-positive and GFP-negative granule cells in the fascia dentata of the Thy1-GFP mouse.


:Granule cells of the mouse fascia dentata are widely used in studies on neuronal development and plasticity. In contrast to the rat, however, high-resolution morphometric data on these cells are scarce. Thus, we have analyzed granule cells in the fascia dentata of the adult Thy1-GFP mouse (C57BL/6 background). In this mouse line, single neurons in the granule cell layer are GFP-labeled, making them amenable to high-resolution 3D-reconstruction. First, calbindin or parvalbumin-immunofluorescence was used to identify GFP-positive cells as granule cells. Second, the dorsal-ventral distribution of GFP-positive granule cells was studied: In the dorsal part of the fascia dentata 11% and in the ventral part 15% of all granule cells were GFP-positive. Third, GFP-positive and GFP-negative granule cells were compared using intracellular dye-filling (fixed slice technique) and patch-clamp recordings; no differences were observed between the cells. Finally, GFP-positive granule cells (dorsal and ventral fascia dentata) were imaged at high resolution with a confocal microscope, 3D-reconstructed in their entirety and analyzed for soma size, total dendritic length, number of segments, total number of spines and spine density. Sholl analysis revealed that dendritic complexity of granule cells is maximal 150-200 mum from the soma. Granule cells located in the ventral part of the hippocampus revealed a greater degree of dendritic complexity compared to cells in the dorsal part. Taken together, this study provides morphometric data on granule cells of mice bred on a C57BL/6 background and establishes the Thy1-GFP mouse as a tool to study granule cell neurobiology.






Vuksic M,Del Turco D,Bas Orth C,Burbach GJ,Feng G,Müller CM,Schwarzacher SW,Deller T




Has Abstract


2008-01-01 00:00:00












  • Modulation of AMPA receptor-mediated ion current by pituitary adenylate cyclase-activating polypeptide (PACAP) in CA1 pyramidal neurons from rat hippocampus.

    abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP), a neurotrophic and neuromodulatory peptide, was recently shown to enhance NMDA receptor-mediated currents in the hippocampus (Macdonald, et al. 2005. J Neurosci 25:11374-11384). To check if PACAP might also modulate AMPA receptor function, we tested its effec...


    pub_type: 杂志文章


    authors: Costa L,Santangelo F,Li Volsi G,Ciranna L

    更新日期:2009-01-01 00:00:00

  • Aberrant synaptic transmission in the hippocampal CA3 region and cognitive deterioration in protein-repair enzyme-deficient mice.

    abstract::L-aspartate is the amino-acid residue most susceptible to spontaneous isomerization. This denaturation causes an alteration in the biological activity of the protein and is regarded as an aging process of the protein. Protein L-isoaspartyl methyltransferase (PIMT) repairs this post-translational modification and thus ...


    pub_type: 杂志文章


    authors: Ikegaya Y,Yamada M,Fukuda T,Kuroyanagi H,Shirasawa T,Nishiyama N

    更新日期:2001-01-01 00:00:00

  • Intact verbal and nonverbal short-term memory following damage to the human hippocampus.

    abstract::Short-term memory was assessed in two groups of amnesic patients. Six patients had confirmed or suspected damage to the hippocampal formation, and six patients had diencephalic damage as a result of alcoholic Korsakoff's syndrome. Verbal short-term memory was evaluated with seven separate administrations of the standa...


    pub_type: 杂志文章


    authors: Cave CB,Squire LR

    更新日期:1992-04-01 00:00:00

  • Running exercise-induced up-regulation of hippocampal brain-derived neurotrophic factor is CREB-dependent.

    abstract::The past decade has witnessed burgeoning evidence that antidepressant medications and physical exercise increase the expression of hippocampal brain-derived neurotrophic factor (BDNF). This phenomenon has gained widespread appeal, because BDNF is one of the first macromolecules observed to play a central role not only...


    pub_type: 杂志文章


    authors: Chen MJ,Russo-Neustadt AA

    更新日期:2009-10-01 00:00:00

  • Quantal and subquantal GABAergic transmissions in cultured rat hippocampal neurons.

    abstract::At the neuromuscular junction, spontaneous miniature excitatory synaptic currents mediated by acetylcholine are considered elementary, "quantal" transmissions. These miniature conductances can be quantitatively dichotomized into a large-mode class whose mode is the mean of a normal, bell-shaped distribution and a smal...


    pub_type: 杂志文章


    authors: Vautrin J,Schaffner AE,Barker JL

    更新日期:1993-01-01 00:00:00

  • Relational learning and transitive expression in aging and amnesia.

    abstract::Aging has been associated with a decline in relational memory, which is critically supported by the hippocampus. By adapting the transitivity paradigm (Bunsey and Eichenbaum (1996) Nature 379:255-257), which traditionally has been used in nonhuman animal research, this work examined the extent to which aging is accomp...


    pub_type: 杂志文章


    authors: Ryan JD,D'Angelo MC,Kamino D,Ostreicher M,Moses SN,Rosenbaum RS

    更新日期:2016-02-01 00:00:00

  • Distal landmarks and hippocampal place cells: effects of relative translation versus rotation.

    abstract::Hippocampal neurons are selectively active when a rat occupies restricted locations in an environment. These place cells derive their specificity from a multitude of sources, including idiothetic cues and sensory input derived from both distal and local landmarks. Most experiments have attempted to dissociate the rela...


    pub_type: 杂志文章


    authors: Knierim JJ,Rao G

    更新日期:2003-01-01 00:00:00

  • Hippocampal lesions enhance configural learning by reducing proactive interference.

    abstract::Rats were trained on an operant conditional discrimination in which an ambiguous stimulus (X) indicated both the occasions on which responding in the presence of a second cue (A) would be reinforced and the occasions on which responding in the presence of a third cue (B) would not be reinforced (X --> A+, A-, X --> B-...


    pub_type: 杂志文章


    authors: Han JS,Gallagher M,Holland P

    更新日期:1998-01-01 00:00:00

  • A computational model of systems memory consolidation and reconsolidation.

    abstract::In the mammalian brain, newly acquired memories depend on the hippocampus (HPC) for maintenance and recall, but over time, the neocortex takes over these functions, rendering memories HPC-independent. The process responsible for this transformation is called systems memory consolidation. Reactivation of a well-consoli...


    pub_type: 杂志文章


    authors: Helfer P,Shultz TR

    更新日期:2020-07-01 00:00:00

  • Human hippocampal theta activity during virtual navigation.

    abstract::This study examines whether 4-8-Hz theta oscillations can be seen in the human hippocampus, and whether these oscillations increase during virtual movement and searching, as they do in rodents. Recordings from both hippocampal and neocortical depth electrodes were analyzed while six epileptic patients played a virtual...


    pub_type: 杂志文章


    authors: Ekstrom AD,Caplan JB,Ho E,Shattuck K,Fried I,Kahana MJ

    更新日期:2005-01-01 00:00:00

  • Changes in protein kinase C (PKC) activity, isozyme translocation, and GAP-43 phosphorylation in the rat hippocampal formation after a single-trial contextual fear conditioning paradigm.

    abstract::The hippocampus plays an important role in spatial learning and memory. However, the biochemical alterations that subserve this function remain to be fully elucidated. In this study, rats were subjected to a single-trial contextual fear conditioning (CFC) paradigm; the activation of different protein kinase C (PKC) su...


    pub_type: 杂志文章


    authors: Young E,Cesena T,Meiri KF,Perrone-Bizzozero NI

    更新日期:2002-01-01 00:00:00

  • A harmonized segmentation protocol for hippocampal and parahippocampal subregions: Why do we need one and what are the key goals?

    abstract::The advent of high-resolution magnetic resonance imaging (MRI) has enabled in vivo research in a variety of populations and diseases on the structure and function of hippocampal subfields and subdivisions of the parahippocampal gyrus. Because of the many extant and highly discrepant segmentation protocols, comparing r...


    pub_type: 杂志文章


    authors: Wisse LEM,Daugherty AM,Olsen RK,Berron D,Carr VA,Stark CEL,Amaral RSC,Amunts K,Augustinack JC,Bender AR,Bernstein JD,Boccardi M,Bocchetta M,Burggren A,Chakravarty MM,Chupin M,Ekstrom A,de Flores R,Insausti R,Kanel P

    更新日期:2017-01-01 00:00:00

  • Ethanol, memory, and hippocampal function: a review of recent findings.

    abstract::For well over a century, ethanol was believed to exert its effects on cognition and behavior by producing a ubiquitous depression of central nervous system activity. A general disruption in brain function was consistent with the belief that ethanol's effects on cognition and behavior were also quite general. Substanti...


    pub_type: 杂志文章,评审


    authors: White AM,Matthews DB,Best PJ

    更新日期:2000-01-01 00:00:00

  • Aging and KIBRA/WWC1 genotype affect spatial memory processes in a virtual navigation task.

    abstract::Spatial navigation relies on multiple mnemonic mechanisms and previous work in younger adults has described two separate types of spatial memory. One type uses directional as well as boundary-related information for spatial memory and mainly implicates the hippocampal formation. The other type has been linked to direc...


    pub_type: 杂志文章


    authors: Schuck NW,Doeller CF,Schjeide BM,Schröder J,Frensch PA,Bertram L,Li SC

    更新日期:2013-10-01 00:00:00

  • A new method for the in vivo volumetric measurement of the human hippocampus with high neuroanatomical accuracy.

    abstract::Accurate and reproducible in vivo measurement of hippocampal volumes using magnetic resonance (MR) imaging is complicated by the morphological complexity of the structure. Additionally, separation of certain parts of the hippocampus from the adjacent brain structures on MR images is sometimes very difficult. These dif...


    pub_type: 临床试验,杂志文章


    authors: Pantel J,O'Leary DS,Cretsinger K,Bockholt HJ,Keefe H,Magnotta VA,Andreasen NC

    更新日期:2000-01-01 00:00:00

  • Social isolation prevents exercise-induced proliferation of hippocampal progenitor cells in female rats.

    abstract::Social isolation negatively affects the behavior and health of laboratory rats. Recently, it has been found that social isolation retards exercise-induced neurogenesis in the hippocampal dentate gyrus (DG) of male rats (Stranahan et al. (2006) Nat Neurosci 9:526-533). Since male and female rats react differently to ho...


    pub_type: 杂志文章


    authors: Leasure JL,Decker L

    更新日期:2009-10-01 00:00:00

  • Excitation/inhibition imbalance and impaired synaptic inhibition in hippocampal area CA3 of Mecp2 knockout mice.

    abstract::Rett syndrome (RTT) is a neurodevelopment disorder associated with intellectual disabilities and caused by loss-of-function mutations in the gene encoding the transcriptional regulator Methyl-CpG-binding Protein-2 (MeCP2). Neuronal dysfunction and changes in cortical excitability occur in RTT individuals and Mecp2-def...


    pub_type: 杂志文章


    authors: Calfa G,Li W,Rutherford JM,Pozzo-Miller L

    更新日期:2015-02-01 00:00:00

  • Septohippocampal pathways contribute to system consolidation of a spatial memory: sequential implication of GABAergic and cholinergic neurons.

    abstract::Studies of the neuropharmacological substrates of spatial memory formation have focused on the contribution of septohippocampal pathways. Although these pathways include, among others, cholinergic and GABAergic fibers innervating the hippocampus, research has essentially been oriented towards the role of their choline...


    pub_type: 杂志文章


    authors: Lecourtier L,de Vasconcelos AP,Leroux E,Cosquer B,Geiger K,Lithfous S,Cassel JC

    更新日期:2011-12-01 00:00:00

  • Reminders activate the prefrontal-medial temporal cortex and attenuate forgetting of event memory.

    abstract::Replicas of an aspect of an experienced event can serve as effective reminders, yet little is known about the neural basis of such reminding effects. Here we examined the neural activity underlying the memory-enhancing effect of reminders 1 week after encoding of naturalistic film clip events. We used fMRI to determin...


    pub_type: 杂志文章


    authors: Sekeres MJ,Moscovitch M,Winocur G,Pishdadian S,Nichol D,Grady CL

    更新日期:2021-01-01 00:00:00

  • PTU-induced hypothyroidism in rats leads to several early neuropathological signs of Alzheimer's disease in the hippocampus and spatial memory impairments.

    abstract::The multifactorial causes impacting the risk of developing sporadic forms of Alzheimer's disease (AD) remain to date poorly understood. Epidemiologic studies in humans and research in rodents have suggested that hypothyroidism could participate in the etiology of AD. Recently, we reported that adult-onset hypothyroidi...


    pub_type: 杂志文章


    authors: Chaalal A,Poirier R,Blum D,Gillet B,Le Blanc P,Basquin M,Buée L,Laroche S,Enderlin V

    更新日期:2014-11-01 00:00:00

  • The relationship between hippocampal subfield volumes and autobiographical memory persistence.

    abstract::Structural integrity of the human hippocampus is widely acknowledged to be necessary for the successful encoding and retrieval of autobiographical memories. However, evidence for an association between hippocampal volume and the ability to recall such memories in healthy individuals is mixed. Here we examined this iss...


    pub_type: 杂志文章


    authors: Barry DN,Clark IA,Maguire EA

    更新日期:2020-12-15 00:00:00

  • Awareness predicts the magnitude of single-cue trace eyeblink conditioning.

    abstract::Studies of differential eyeblink conditioning (CS+ and CS-) have demonstrated that successful conditioning requires awareness of the stimulus contingencies and that delay conditioning does not. Two experiments were carried out to determine whether awareness is also important for single-cue trace eyeblink conditioning....


    pub_type: 杂志文章


    authors: Manns JR,Clark RE,Squire LR

    更新日期:2000-01-01 00:00:00

  • Study of CA1 place cell activity and exploratory behavior following spatial and nonspatial changes in the environment.

    abstract::Changes in the spatial arrangement or identity of objects inside a familiar environment induce reexploration. The present study looks at modifications of place cell activity during such renewed exploration. Hungry rats foraged for food in a cylinder with a salient cue card attached to the wall and with two distinct ob...


    pub_type: 杂志文章


    authors: Lenck-Santini PP,Rivard B,Muller RU,Poucet B

    更新日期:2005-01-01 00:00:00

  • Reliving lifelong episodic autobiographical memories via the hippocampus: a correlative resting PET study in healthy middle-aged subjects.

    abstract::We aimed at identifying the cerebral structures whose synaptic function subserves the recollection of lifetime's episodic autobiographical memory (AM) via autonoetic consciousness. Twelve healthy middle-aged subjects (mean age: 59 years +/- 2.5) underwent a specially designed cognitive test to assess the ability to re...


    pub_type: 杂志文章


    authors: Piolino P,Desgranges B,Hubert V,Bernard FA,Matuszewski V,Chételat G,Baron JC,Eustache F

    更新日期:2008-01-01 00:00:00

  • Cooperation between cholinergic and glutamatergic receptors are essential to induce BDNF-dependent long-lasting memory storage.

    abstract::The induction of long-lasting memory storage depends on the behavioral state of humans and animals. This behavioral state is mediated by neuromodulatory systems, like the cholinergic-septum-hippocampal circuit. Cholinergic neurotransmission is known to affect short-term activity-dependent plasticity in various brain a...


    pub_type: 杂志文章


    authors: Navakkode S,Korte M

    更新日期:2012-02-01 00:00:00

  • Synaptophysin protein and mRNA expression in the human hippocampal formation from birth to old age.

    abstract::In the human neocortex, progressive synaptogenesis in early postnatal life is followed by a decline in synaptic density, then stability from adolescence until middle age. No comparable data are available in the hippocampus. In this study, the integral synaptic vesicle protein synaptophysin, measured immunoautoradiogra...


    pub_type: 杂志文章


    authors: Eastwood SL,Weickert CS,Webster MJ,Herman MM,Kleinman JE,Harrison PJ

    更新日期:2006-01-01 00:00:00

  • Amygdala-kindling induces alterations in neuronal density and in density of degenerated fibers.

    abstract::Kindling is characterized by a progressive intensification of seizure activity culminating in generalized seizures following repeated administration of an initially subconvulsive electrical or chemical stimulus. Since it is known that epilepsy induces morphological alterations in the limbic system, we examined the neu...


    pub_type: 杂志文章


    authors: von Bohlen und Halbach O,Schulze K,Albrecht D

    更新日期:2004-01-01 00:00:00

  • Genetic ablation of tenascin-C expression leads to abnormal hippocampal CA1 structure and electrical activity in vivo.

    abstract::Despite evidence that the extracellular matrix glycoprotein tenascin-C (TNC) is implicated in brain development and plasticity, its roles in the intact adult brain are unknown. Here we report that spontaneous local field potential (LFP) activity in freely moving adult TNC-deficient mice is abnormal. The power of corti...


    pub_type: 杂志文章


    authors: Gurevicius K,Kuang F,Stoenica L,Irintchev A,Gureviciene I,Dityatev A,Schachner M,Tanila H

    更新日期:2009-12-01 00:00:00

  • Retrieval flexibility and reinstatement in the developing hippocampus.

    abstract::Episodic memory improves during childhood and this improvement has been associated with age differences in hippocampal function, but previous research has not manipulated the possible underlying mechanisms. We tested the hypothesis that age-related differences in hippocampal activation may reflect changes in retrieval...


    pub_type: 杂志文章


    authors: DeMaster D,Coughlin C,Ghetti S

    更新日期:2016-04-01 00:00:00

  • Hippocampal place cells: the "where" of episodic memory?

    abstract::Neuropsychologists have clearly implicated the hippocampus in the consolidation of memory, particularly episodic memory, the mental replay of past experiences. When recorded from behaving animals, by far the most obvious firing pattern of the primary neurons of the hippocampus is the place field: a cell tends to fire ...


    pub_type: 杂志文章,评审


    authors: Kentros C

    更新日期:2006-01-01 00:00:00