Abstract:
BACKGROUND AND AIMS:The detrimental impact of opioid agonist on the clinical management of inflammatory diseases remains elusive. Given the anti-inflammatory properties of the mu-opioid receptor (MOR) agonists at the intestinal barrier, we hypothesised that MOR activation might also dampen acute hepatic inflammation and cell death-major determinants in the pathogenesis of liver diseases. PATIENTS AND METHODS:The expression of MOR in liver biopsy specimens and peripheral blood mononuclear cells of untreated patients with chronic hepatitis C virus infection and controls, primary hepatocytes and cell lines was determined by quantitative PCR, immunoblotting and/or immunohistochemistry. The effects of peripheral MOR agonist (d-Ala2,NMe-Phe4,Gly5-ol (DAMGO)) and/or antagonist (naloxone methiodide) were explored in two models of acute hepatitis in mice. MOR-deficient mice were used to evaluate the essential regulatory role of MOR during carbon tetrachloride (CCl(4))-induced hepatitis. The role of DAMGO in cell death was investigated using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) analysis and quantification of lactate dehydrogenase release. RESULTS:The key role of MOR in the prevention of acute hepatic inflammation and cell death in vivo and in vitro is reported. Whereas MOR gene expression increased transiently in the model of acute liver injury and TNFalpha-treated HepG2 cells, an impaired expression of MOR mRNA in human chronic hepatitis C samples was found. Furthermore, preventive administration of the selective MOR agonist DAMGO enhanced hepatoprotective-signalling pathways in vivo that were blocked by using naloxone methiodide. Consistently, genetic and pharmacological inhibition of MOR enhanced the severity associated with experimental hepatotoxin-induced hepatitis. Finally, treatment with DAMGO was shown to prevent cell death in vitro in HepG2 cells in a MOR-dependent manner and to prevent concanavalin A- and CCl(4)-induced cell death in vivo, providing a possible explanation for the anti-inflammatory role of MOR activation in the liver. CONCLUSIONS:The results indicate that MOR agonists may prevent acute hepatitis and hold promising therapeutic use to maintain remission in both chronic inflammatory bowel and liver diseases.
journal_name
Gutjournal_title
Gutauthors
Chakass D,Philippe D,Erdual E,Dharancy S,Malapel M,Dubuquoy C,Thuru X,Gay J,Gaveriaux-Ruff C,Dubus P,Mathurin P,Kieffer BL,Desreumaux P,Chamaillard Mdoi
10.1136/gut.2006.105122subject
Has Abstractpub_date
2007-07-01 00:00:00pages
974-81issue
7eissn
0017-5749issn
1468-3288pii
gut.2006.105122journal_volume
56pub_type
杂志文章相关文献
GUT文献大全abstract::Amino acid induced acid and gastrin responses during intragastric titration at pH 2.5 and 5.5 were compared in normal subjects and duodenal ulcer patients with G-cell hyperfunction. The latter were identified on the basis of raised basal or maximal acid outputs and increased gastrin responses to feeding. In normal sub...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.3.232
更新日期:1985-03-01 00:00:00
abstract::Pectin has been shown to minimise the fall in blood glucose seen in patients who are troubled by hypoglycaemia attacks after gastric surgery. We therefore performed 50 g glucose tolerance tests with and without 14.5 g pectin on 11 post-gastric surgery patients. After pectin, the high postprandial levels of glucose, in...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.21.7.574
更新日期:1980-07-01 00:00:00
abstract::A dodecapeptide of A-gliadin, which shares amino acid homologies with the E1b protein of adenovirus 12, was used to produce murine monoclonal antibodies. Five monoclonal antibodies were produced and were screened by enzyme linked immunosorbant assay, immunodot assay, and immunoblotting. The antibodies were tested agai...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.33.11.1504
更新日期:1992-11-01 00:00:00
abstract::Twenty healthy subjects eating normal diets made repeated five-day stool collections, the 10 females making their collections in four to six successive weeks. In most subjects there were striking variations in transit time, measured by Hinton's method. The variability of average faecal wet and dry weight, faecal volum...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.19.2.146
更新日期:1978-02-01 00:00:00
abstract::A 69 year old woman developed chronic diarrhoea while being treated with ranitidine. Sigmoidoscopy was performed after six weeks and showed typical histological features of lymphocytic colitis. Ranitidine was withdrawn and the diarrhoea resolved. Eight months later, a 72 hour oral rechallenge period of ranitidine, was...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.37.5.708
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND:The potential of endoscopic mucosal resection (EMR) for treating flat dysplastic lesions in chronic ulcerative colitis (CUC) has not been addressed so far. Historically, such lesions were referred for colectomy. Furthermore, there are only limited data to support endoscopic resection of exophytic adenoma-lik...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2006.106294
更新日期:2007-06-01 00:00:00
abstract::Our life expectancy is increasing, leading to a rise in the ageing population. Ageing is associated with a decline in physiological function and adaptive capacity. Altered GI physiology can affect the amount and types of nutrients digested and absorbed as well as impact the intestinal microbiota. The intestinal microb...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gutjnl-2017-315542
更新日期:2018-12-01 00:00:00
abstract::Forty five patients with refractory oesophagitis, defined as persisting erosive changes or ulceration despite a minimum of three months' treatment with cimetidine 3.2 g daily or ranitidine 0.9 g daily, were treated in an open trial with omeprazole 40 mg daily for up to eight weeks. Endoscopically defined healing was o...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.31.7.745
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND:Increased expression of proinflammatory cytokines, including tumour necrosis factor alpha, interleukin 6, and interferon gamma, as well as activation of proinflammatory signalling molecules such as nuclear factor kappa B, is characteristic of inflammatory bowel disease (IBD). AIMS:To investigate expression ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.51.3.379
更新日期:2002-09-01 00:00:00
abstract::Using immune electron microscopy, an attempt has been made to visualise viral particles in Crohn's disease tissue and faeces. No particles resembling viruses were observed, but in the absence of a specific antiserum a negative result does not exclude the presence of a virus, particularly if it is sparsely distributed....
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.17.9.696
更新日期:1976-09-01 00:00:00
abstract:BACKGROUND:Previous studies of anticipation in familial pancreatic cancer have been small and subject to ascertainment bias. Our aim was to determine evidence for anticipation in a large number of European families. PATIENTS AND METHODS:A total of 1223 individuals at risk from 106 families (264 affected individuals) w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2005.065045
更新日期:2006-02-01 00:00:00
abstract:OBJECTIVES:Screening colonoscopy to monitor for early colitis-associated colon cancer (CAC) is difficult due to the aberrant mucosal patterns associated with long-standing colitis. The aim of this study was to develop a rapid fluorescent detection method for use during colonoscopy for improving the detection of CAC uti...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2011-301795
更新日期:2013-08-01 00:00:00
abstract:AIMS:To determine the harm that ensues from faecal occult blood (FOB) screening for colorectal cancer. METHODS:150 251 people were randomly allocated either to receive biennial Haemoccult FOB tests (n =75 253) or not to be contacted (n=74 998). Study group patients returning positive tests were offered colonic investi...
journal_title:Gut
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/gut.45.4.588
更新日期:1999-10-01 00:00:00
abstract::The risk of developing colorectal adenomatous polyps is probably increased by a variety of dietary and environmental factors. We found an association with current alcohol and cigarette consumption. The risk of polyps was increased three times in drinkers who did not smoke and two times in smokers who did not drink, wi...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.32.1.70
更新日期:1991-01-01 00:00:00
abstract::This paper describes the construction and use of "radio pills" which transmit changes in intraluminar pressure. ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.1.3.266
更新日期:1960-09-01 00:00:00
abstract:BACKGROUND:In a previous hospital based study, we suggested that undiagnosed coeliac disease has a prevalence, among pregnant women, of 1:80, and is a cause of unfavourable outcome of pregnancy. AIMS:In order to confirm or dismiss this hypothesis, which has significant public health implications, we carried out a larg...
journal_title:Gut
pub_type: 杂志文章,多中心研究
doi:10.1136/gut.53.1.149
更新日期:2004-01-01 00:00:00
abstract::Serum zinc concentrations and urine zinc excretion have been studied in 10 patients with severe Crohn's disease before and during 59 patient-weeks of intravenous nutrition. Before serum zinc concentrations (9.9 +/- 1.0 mumol/l: mean +/- SEM) and urine zinc excretion (3-3 +/- 0.6 mumol/24h) were less than controls (p l...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.23.11.984
更新日期:1982-11-01 00:00:00
abstract:BACKGROUND:Polyamines are essential for cell growth. Dietary and probably gut bacterial derived polyamines contribute significantly to the polyamine body pool. AIMS:To evaluate the influence of dietary, luminal polyamines on growth and development of different gastrointestinal organs in normally growing rats. METHODS...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.44.1.12
更新日期:1999-01-01 00:00:00
abstract::Cholestasis of pregnancy is associated with increased fetal morbidity and mortality and should be treated actively. The significance attached to pruritus in pregnancy is often minimal, but it is a cardinal symptom of cholestasis of pregnancy, which may have no other clinical features. Eight women with previous cholest...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.37.4.580
更新日期:1995-10-01 00:00:00
abstract:CLINICAL PRESENTATION:A 28-year-old woman presented with a 3-year history of chronic watery diarrhoea along with abdominal pain and bloating, which could mostly be alleviated after defecation. Her symptom of diarrhoea, at least three times a day, could be relieved by neither probiotics nor antidiarrhoeal agents. She ha...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2018-316182
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND AND AIM:The treatment of ulcerative colitis (UC) with 5-aminosalicylic acid (5-ASA) does not have the same therapeutic effect in all patients. We tested the hypothesis that the effectiveness of the drug is related to its mucosal concentration. PATIENTS:Twenty one UC patients receiving oral 5-ASA (2.4-3.2 g/...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.47.3.410
更新日期:2000-09-01 00:00:00
abstract::When there is villus atrophy in a jejunal biopsy, intraepithelial lymphocyte (IEL) mitosis correlates with a diagnosis of coeliac disease. We have examined the significance of IEL mitosis in jejunal biopsies with normal villi. Counts of IEL per 100 villus enterocytes, and IEL mitosis per 1000 IEL, were carried out in ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.27.6.675
更新日期:1986-06-01 00:00:00
abstract:OBJECTIVE:Caffeine reduces toxic Ca2+ signals in pancreatic acinar cells via inhibition of inositol 1,4,5-trisphosphate receptor (IP3R)-mediated signalling, but effects of other xanthines have not been evaluated, nor effects of xanthines on experimental acute pancreatitis (AP). We have determined effects of caffeine an...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2015-309363
更新日期:2017-02-01 00:00:00
abstract::Seventeen patients with proven systemic sclerosis had a peroral jejunal biopsy performed. Four biopsies were regarded as showing abnormalities, which were mostly confined to the deeper structures, although in two there was a minimal degree of villous atrophy without epithelial cell changes. Passive intestinal permeabi...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.21.4.293
更新日期:1980-04-01 00:00:00
abstract:OBJECTIVE:Although severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was detected in faeces of patients with COVID-19, the activity and infectivity of the virus in the GI tract during disease course is largely unknown. We investigated temporal transcriptional activity of SARS-CoV-2 and its association wi...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2020-322294
更新日期:2021-02-01 00:00:00
abstract::The responses to deglutition of the lower oesophageal high pressure zone were studied in 13 healthy subjects. The recording assembly included three perfused pressure recording catheters, whose recording orifices were at the same axial level, and a more distally situated pH electrode. Relaxation and contraction progres...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.13.5.352
更新日期:1972-05-01 00:00:00
abstract::The size of the related lymph nodes and their histology has been correlated in 34 specimens of Crohn's disease. Granulomata were shown not to affect the maximum diameter of the lymph nodes nor to be more frequent in large nodes. The enlargement of lymph nodes appeared to be due to simple, non-specific, reactive change...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.13.12.970
更新日期:1972-12-01 00:00:00
abstract:OBJECTIVE:Colorectal cancer (CRC) leads to significant morbidity/mortality worldwide. Defining critical research gaps (RG), their prioritisation and resolution, could improve patient outcomes. DESIGN:RG analysis was conducted by a multidisciplinary panel of patients, clinicians and researchers (n=71). Eight working gr...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gutjnl-2017-315333
更新日期:2018-01-01 00:00:00
abstract::Twenty one patients with diabetic peripheral neuropathy, 18 with idiopathic faecal incontinence and 11 normal controls were studied with techniques of mucosal electrosensitivity, rectal distension for the quantitative assessment of anorectal sensation, and manometric and electromyographic tests for the assessment of a...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.29.6.756
更新日期:1988-06-01 00:00:00
abstract::Results emerging from endoscopic treatments to ablate Barrett's oesophagus indicate that APC alone or ALA-PDT in combination with APC achieves complete clearance of Barrett's epithelium in approximately two thirds of patients. ...
journal_title:Gut
pub_type: 评论,杂志文章
doi:10.1136/gut.2003.036392
更新日期:2004-06-01 00:00:00