Does estrogen alter the mechanical properties of the anterior cruciate ligament? An experimental study in rabbits.

Abstract:

BACKGROUND:It is well known that anterior cruciate ligament (ACL) injuries are commoner in female athletes. Accordingly, we hypothesized that serum estrogen may play some role in this sex difference. We evaluated the relationship between serum estrogen levels and the mechanical properties of the ACL in rabbits. ANIMALS AND METHODS:In 40 ovariectomized rabbits, the serum estrogen levels (SEL) were controlled by intramuscular injection of 17beta-estradiol. The mean SEL in each rabbit was defined as the average of 5 determinations done at 1, 2, 3, 4 and 5 weeks after ovariectomy. The animals were divided into 4 groups according to the dose of estradiol administered (low, medium, high and control: L, M, H and C, respectively) and into 2 groups according to the mean SEL (high-SEL group and low-SEL group). The medial portions of ACL attached to both femur and tibia harvested at 5 weeks after ovariectomy were examined mechanically. RESULTS:The mean serum estrogen levels in groups C, L, M and H were 37, 50, 60 and 231 pg/mL. Statistically significant differences in the mean serum estrogen levels were seen among the 4 groups, except between groups L and M. Statistically significant differences were found between groups M and H in both the ultimate tensile stress and linear stiffness. In the comparison between 2 groups using the mean SEL value, both ultimate tensile stress and linear stiffness were lower in the high-SEL group. In all animals, a positive correlation was found between ultimate tensile stress and linear stiffness. INTERPRETATION:Our findings suggest that high SEL might be one of the factors in the multifactorial pathogenesis of ACL rupture.

journal_name

Acta Orthop

journal_title

Acta orthopaedica

authors

Komatsuda T,Sugita T,Sano H,Kusakabe T,Watanuki M,Yoshizumi Y,Murakami T,Hashimoto M,Kokubun S

doi

10.1080/17453670610013312

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

973-80

issue

6

eissn

1745-3674

issn

1745-3682

pii

770235903

journal_volume

77

pub_type

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