Abstract:
:The aim of this study was to investigate the effects of alginate and agarose on the response of bone marrow stromal cells (BMSCs) to chondrogenic stimuli. Rat BMSCs were expanded in monolayer culture with or without FGF-2 supplementation. Cells were then seeded in 2% alginate and agarose gels and cultured in media with or without TGF-beta1 or dexamethasone (Dex). Sulfated glycosaminoglycans (sGAGs), collagen type II, and aggrecan were expressed in all groups that received TGF-beta1 treatment during hydrogel culture. Expansion of rat BMSCs in the presence of FGF-2 increased production of sGAG in TGF-beta1-treated groups over those cultures that were treated with TGF-beta1 alone in alginate cultures. However, in agarose, cells exposed to FGF-2 during expansion produced less sGAG within TGF-beta1-supplemented groups over those cultures treated with TGF-beta1 alone. Dex was required for optimal matrix synthesis in both hydrogels, but was found to decrease cell viability in agarose constructs. These results indicate that the response of BMSCs to a chondrogenic growth factor regimen is scaffold dependent.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Coleman RM,Case ND,Guldberg REdoi
10.1016/j.biomaterials.2007.01.010subject
Has Abstractpub_date
2007-04-01 00:00:00pages
2077-86issue
12eissn
0142-9612issn
1878-5905pii
S0142-9612(07)00023-3journal_volume
28pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::We investigated the morphological effect of phase-separated block copolymer surfaces composed of poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) and poly(dimethylsiloxane) (PDMS) on protein adsorption and cell adhesion behavior. We observed three different types of phase-separated surface morphologies by T...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.06.031
更新日期:2009-10-01 00:00:00
abstract::The ultimate goal of implantation of biomaterials in the skeleton is to reach full integration of the non-living implant with the living bone. The biomaterial can be used much as a bone graft, resorbing or dissolving as bone growth occurs, and the end result is a new remoulded bone. Calcium pyrophosphate, Ca2P2O7, is ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(95)99642-y
更新日期:1995-07-01 00:00:00
abstract::Hyaluronan (HL), a naturally occurring glycosaminoglycan, has been chemically modified through the esterification of its carboxylic groups with different types of alcohol. The physico-chemical properties of these new biopolymers allow the preparation of many biomaterials which may be used in several medical applicatio...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(93)90160-4
更新日期:1993-12-01 00:00:00
abstract::The ability to restore heart function by replacement of diseased myocardium is one of the great challenges in biomaterials and regenerative medicine. Brown adipose derived stem cells (BADSCs) present a new source of cardiomyocytes to regenerate the myocardium after infarction. In this study, we explored an injectable ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.01.021
更新日期:2014-04-01 00:00:00
abstract::Forty squares of tissue, 40 mm X 40 mm, were harvested from bovine pericardium fixed in glutaraldehyde free of tension. Two parallel dumb-bell specimens were excised from each square. In 20 pairs of adjacent samples, two 1 mm cuts were introduced into one of the samples at the centre of the dumb-bell. The other 20 pai...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(89)90098-7
更新日期:1989-05-01 00:00:00
abstract::Titanium (Ti) osseointegration is critical for the success of dental and orthopedic implants. Previous studies have shown that surface roughness at the micro- and submicro-scales promotes osseointegration by enhancing osteoblast differentiation and local factor production. Only relatively recently have the effects of ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.01.029
更新日期:2011-05-01 00:00:00
abstract::Metal sensitivity (MS) reactions to implant metals represent a rare but well-documented complication following total joint arthroplasty (TJA). Although 20-25% of post-TJA patients develop MS, only a few highly susceptible patients (< 1%) exhibit symptoms. Whether surgeons should perform screening for MS is currently a...
journal_title:Biomaterials
pub_type: 临床试验,杂志文章
doi:10.1016/j.biomaterials.2004.03.038
更新日期:2005-03-01 00:00:00
abstract::The major problem in skin grafting is that tissue-engineered skin grafts after their transplantation are initially entirely dependent on diffusion. Since this process is slow and inefficient, nutrients, growth factors, and oxygen will insufficiently be supplied and the regenerating graft will undergo a physiological c...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.02.049
更新日期:2014-06-01 00:00:00
abstract::Osteoinductive biomaterials are promising for bone repair. There is no direct proof that bone marrow mesenchymal stem cells (BMSCs) home to non-osseous sites and participate in ectopic bone formation induced by osteoinductive bioceramics. The objective of this study was to use a sex-mismatched beagle dog model to inve...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.12.010
更新日期:2013-03-01 00:00:00
abstract::Micro-computed tomography (micro-CT) is an emerging imaging modality, due to the low cost of the imagers as well as their efficiency in establishing high-resolution (1-100 μm) three-dimensional images of small laboratory animals and facilitating rapid, structural and functional in vivo visualization. However use of a ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.09.026
更新日期:2013-01-01 00:00:00
abstract::Surface biofouling poses an increasing problem in industrial and health care applications, driving research for surface coatings to prevent anti-microbial colonization and characterization of the efficacy of the same. The diversity and increasing sophistication of such coatings, which postulate different types of anti...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.02.063
更新日期:2011-07-01 00:00:00
abstract::The long-term patency rates of vascular grafts and stents are limited by the lack of surface endothelialisation of the implanted materials. We have previously reported that GRGDS and WQPPRARI peptide micropatterns increase the endothelialisation of prosthetic materials in vitro. To investigate the mechanisms by which ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.09.076
更新日期:2014-01-01 00:00:00
abstract::A major obstacle in the survival and efficacy of tissue engineered transplants is inadequate oxygenation, whereby unsupportive oxygen tensions result in significant cellular dysfunction and death within the implant. In a previous report, we developed an innovative oxygen generating biomaterial, termed OxySite, to prov...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.03.018
更新日期:2017-06-01 00:00:00
abstract::Biological heart prostheses present medium and long-term problems, owing to progressive deterioration and calcification of the tissue. To study the latter problem, we compared the effectiveness of anticalcification treatments in calf pericardium samples implanted into female Wistar rats after the following procedures:...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90208-r
更新日期:1991-03-01 00:00:00
abstract::Disruption of peripheral nerves due to trauma is a frequently occurring clinical problem. Gaps in the nerve are bridged by guiding the regenerating nerves along autologous grafts or artificial guides. This review gives an overview on the different methods of nerve repair techniques. Conventional suturing techniques ar...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/s0142-9612(03)00504-0
更新日期:2004-04-01 00:00:00
abstract::To achieve an optimum drug delivery such as targeting or controlled release utilizing bioconjugation with polymeric modifier, the conjugate between drugs and polymeric modifiers must be designed to show desirable pharmacokinetic characteristics in vivo. In this study, we assessed the biopharmaceutical properties of va...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.10.003
更新日期:2004-07-01 00:00:00
abstract::The development of electrospun ultrafine fibres from biodegradable and biocompatible polymers has created exciting opportunities for biomedical applications. Fibre meshes with high surface area, suitable porosity and stiffness have been produced. Despite desirable structural and topographical properties, for most synt...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2016.08.011
更新日期:2016-11-01 00:00:00
abstract::Polyurethane microspheres having diameters in the range 150-1500 microns were prepared by condensation polymerization of toluene diisocyanate (TDI) with poly(tetramethylene glycol) (PTMG) of average mol wt 990 in an aqueous dispersion medium containing dioctyl sulphosuccinate (DOS) as the suspension stabilizer and 1,4...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90154-3
更新日期:1991-07-01 00:00:00
abstract::Developing repair strategies for osteochondral tissue presents complex challenges due to its interfacial nature and complex zonal structure, consisting of subchondral bone, intermediate calcified cartilage and the superficial cartilage regions. In this study, the long term ability of a multi-layered biomimetic collage...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.02.006
更新日期:2016-05-01 00:00:00
abstract::Artemisinin (ART) is an iron-dependent anti-cancer drug. However, simultaneous delivery of hydrophobic ART and Fe(2+) ions into cancer cells remains a major challenge. Herein, we reported Fe3O4@C/Ag@mSiO2 (FCA@mSiO2) multifunctional nanocarriers which can load ART as high as 484 mg/g. Moreover, FCA@mSiO2 nanoparticles...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.04.028
更新日期:2014-08-01 00:00:00
abstract::The successful delivery of anti-cancer drugs relies on the simultaneous capability to actively target a specific location, a sufficient lifetime in the active form in the circulation, and traceability and quantification of drug distribution via in vivo medical imaging. Herein, a highly magnetic nanocarrier (HMNC) comp...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.05.047
更新日期:2011-09-01 00:00:00
abstract::The degree of metal ion dissolution from Ti-6Al-4V alloy hip replacement stems subjected to various mechanical and chemical surface pretreatments was analysed in vitro. High-dissolution rates were observed for nitric acid passivated samples that had been mechanically surface treated to increase the implant surface are...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(99)00200-8
更新日期:2000-02-01 00:00:00
abstract::A range of poly(alkyl methacrylate)s in the range C5 to C13 with varying degrees of crosslinking, have been studied with respect to stress-strain behaviour. Where the extensions to break were sufficiently high, stress-strain properties conformed well to the statistical theory of rubber elasticity, the Mooney/Rivlin C2...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(87)90011-1
更新日期:1987-09-01 00:00:00
abstract::There is currently no effective treatment for the retinal disorders caused by retinal pigment epithelium (RPE) degeneration. Transplantation of allografts is the main strategy towards correction of this malady. Tissue engineering could offer hope and involve the use of biodegradable polymeric templates to replace dise...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00302-8
更新日期:2003-11-01 00:00:00
abstract::Hydrogels have been widely used for 3-dimensional (3D) cell culture and tissue regeneration due to their tunable biochemical and physicochemical properties as well as their high water content, which resembles the aqueous microenvironment of the natural extracellular matrix. While many properties of natural hydrogel ma...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.12.081
更新日期:2014-03-01 00:00:00
abstract::To maintain enzymatic activity during long-term storage by conjugation with water-soluble 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers (PMPC-COOH) having various molecular weights with a carboxylic group on the terminal, such compounds were synthesized as a polymer modifier using a photoinduced living radic...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00474-5
更新日期:2004-01-01 00:00:00
abstract::Efficacy of different chemical treatments on calcification of vascular graft in vitro and in vivo was studied. Culture medium-filled rat aortas were separately treated in 0.2% glutaraldehyde and epoxy compound, and photooxidized in 0.01% methylene blue for a shorter period (group 1). Another group of rat aortas were s...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(98)00038-6
更新日期:1998-09-01 00:00:00
abstract::Anti-VEGF drugs that are used in conjunction with laser ablation to treat patients with diabetic retinopathy suffer from short half-lives in the vitreous of the eye resulting in the need for frequent intravitreal injections. To improve the intravitreal half-life of anti-VEGF drugs, such as the VEGF decoy receptor sFlt...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.03.017
更新日期:2016-07-01 00:00:00
abstract::The hydrophilic nature of hydrogel matrices makes them disadvantageous to entrap poorly soluble therapeutic agents and greatly restricts their applications as drug-delivery systems. In this study, we demonstrated that sustained delivery of lipophilic drugs in hydrogel-based devices can be readily achieved by enhancing...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.12.047
更新日期:2009-04-01 00:00:00
abstract::Virus-based nanoparticles have shown promise as vehicles for delivering therapeutic genes. However, the rational design of viral vectors that enable selective tropism towards particular types of cells and tissues remains challenging. Here, we explored structural-functional relationships of the adeno-associated virus 2...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.11.066
更新日期:2016-02-01 00:00:00