Abstract:
:The ubiquitin-like conjugation reactions, ATG8/microtubule-associated protein 1 light chain 3/MAP1LC3 (LC3) to phosphatidylethanolamine (PE) and ATG12 to ATG5, are biochemical hallmarks for autophagy, a cellular process that degrades bulk cellular proteins and organelles. The two conjugation reactions share the same E1-like enzyme ATG7 but have different E2-like enzymes, ATG3 for LC3-PE and ATG10 for ATG12-ATG5. In cells, ATG12-ATG5 conjugation appears to be required for LC3-PE conjugation. Previously, in vitro reconstitution of LC3-PE conjugation, but not the upstream ATG12-ATG5 conjugation, was reported. In this study, we describe for the first time the de novo reconstitution of mammalian ATG12-ATG5 conjugation by using purified recombinant proteins. We show that ATG7, ATG10 and ATP as an energy source are all essential for ATG12-ATG5 conjugation, and mutation of the specific lysine residue of ATG5 for ATG12 conjugation abrogates the reaction. Furthermore, a potent stimulating activity for ATG12-ATG5 conjugation was detected in mammalian cell extracts, and was surprisingly identified as ribosomes. Our detail biochemical analyses indicate that the ribonucleic acid (RNA) component of ribosomes is both necessary and sufficient for this stimulation.
journal_name
Autophagyjournal_title
Autophagyauthors
Shao Y,Gao Z,Feldman T,Jiang Xdoi
10.4161/auto.3270subject
Has Abstractpub_date
2007-01-01 00:00:00pages
10-6issue
1eissn
1554-8627issn
1554-8635pii
3270journal_volume
3pub_type
杂志文章相关文献
Autophagy文献大全abstract::Autophagosome formation is a complex cellular process, which requires major membrane rearrangements leading to the creation of a relatively large double-membrane vesicle that directs its contents to the lysosome for degradation. Although various membrane compartments have been identified as sources for autophagosomal ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.21486
更新日期:2012-11-01 00:00:00
abstract::The yeast serine threonine kinase Atg1 appears to be a key regulator of autophagy and its kinase activity is crucial for autophagy induction. Recent reports have indicated that a mammalian Atg1 homolog, UNC-51-like kinase (ULK) 1, is required for autophagy. We found that ULK1 localizes to the autophagic isolation memb...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.1.7180
更新日期:2009-01-01 00:00:00
abstract::One of the major side effects of cisplatin chemotherapy is toxic acute kidney injury due to preferential accumulation of cisplatin in renal proximal tubule epithelial cells and the subsequent injury to these cells. Apoptosis is known as a major mechanism of cisplatin-induced cell death in renal tubular cells. We have ...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.6309
更新日期:2008-07-01 00:00:00
abstract::To facilitate the purification of rat liver autophagosomes, isolated rat hepatocytes are first incubated for 2 h at 37°C with vinblastine, which induces autophagosome accumulation by blocking the fusion of these organelles with endosomes and lysosomes. The hepatocytes are then electrodisrupted and homogenized, and the...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6.4.11272
更新日期:2010-05-01 00:00:00
abstract::BBC3 (BCL2 binding component 3) is a known apoptosis inducer; however, its role in microglial survival remains poorly understood. In addition to the classical transcription factor TRP53, Mir143 is involved in BBC3 expression at the post-transcriptional level. Here, we identify unique roles of Mir143-BBC3 in mediating ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1191723
更新日期:2016-09-01 00:00:00
abstract::Numerous studies have reported that inhibition of MTOR (mechanistic target of rapamycin kinase) clearly reduces Alzheimer disease neuropathological hallmarks in mouse models. This has resulted in calls for the use of the MTOR inhibitor rapamycin for the treatment of dementia in humans. Unfortunately, intervention with...
journal_title:Autophagy
pub_type: 评论,杂志文章
doi:10.1080/15548627.2019.1615823
更新日期:2019-08-01 00:00:00
abstract::Mechanisms to protect against viral infections are crucial during pregnancy as maternal-fetal transmission can have serious pathological outcomes, including fetal infection and its sequelae, such as growth restriction, birth defects, and/or fetal death. The trophoblast forms the interface between the feto-placental un...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.26558
更新日期:2013-12-01 00:00:00
abstract::Autophagy plays critical roles in plant responses to stress. In contrast to the wealth of information concerning the core process of plant autophagosome assembly, our understanding of the regulation of autophagy is limited. In this study, we demonstrated that transcription factor HsfA1a played a critical role in tomat...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1098798
更新日期:2015-11-02 00:00:00
abstract::The accumulation of ubiquitin-positive protein aggregates has been implicated in the pathogenesis of neurodegenerative diseases, heart disease and diabetes. Emerging evidence indicates that the autophagy lysosomal pathway plays a critical role in the clearance of ubiquitin aggregates, a process that is mediated by the...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6.5.12189
更新日期:2010-07-01 00:00:00
abstract::When no supply of environmental nutrients is available, cells induce autophagy, thereby generating a source of emergency metabolic substrates and energy to maintain the basal cellular activity needed for survival. This autophagy response to starvation has been well characterized in various multicellular organisms, inc...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.4.8171
更新日期:2009-05-01 00:00:00
abstract::Reactive oxygen species (ROS) that accumulate under oxidative pressure cause severe damage to cellular components, and induce various cellular responses, including apoptosis, programmed necrosis and autophagy, depending on the cellular setting. Various studies have described ROS-induced autophagy, but only a few direc...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.19288
更新日期:2012-03-01 00:00:00
abstract::Autophagy genes were first identified in the yeast system and some of their mammalian orthologues have also been characterized. Increasing lines of evidence indicate that various intracellular proteins, including G proteins, mammalian target of rapamycin (mTor) and Pl3K/Akt/PKB, of transmembrane signaling pathways are...
journal_title:Autophagy
pub_type: 评论,杂志文章,评审
doi:10.4161/auto.3427
更新日期:2007-01-01 00:00:00
abstract::Chronic drinking leads to myocardial contractile dysfunction where ethanol metabolism plays an essential role. Acetaldehyde, the main ethanol metabolite, mediates alcohol-induced cell injury although the underlying mechanism is still elusive. This study was designed to examine the mechanism involved in accelerated eth...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.18997
更新日期:2012-04-01 00:00:00
abstract::Autophagy acts as an intrinsic defense system against intracellular bacterial survival. Recently, multiple cellular pathways that target intracellular bacterial pathogens to autophagy have been described. These include the Atg5/LC3 pathway, which targets Shigella, the ubiquitin (Ub)-NDP52-LC3 pathway, which targets Gr...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.7.3.14581
更新日期:2011-03-01 00:00:00
abstract::The ubiquitin-proteasome system (UPS) and the autophagy-lysosomal pathway (ALP) are the two most important components of cellular mechanisms for protein degradation. In the present study we investigated the functional relationship of the two systems and the interactional role of p53 in vitro. Our study showed that the...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.5.8377
更新日期:2009-07-01 00:00:00
abstract::Non-small cell lung cancer (NSCLC) often metastasizes to the brain, but identifying which patients will develop brain metastases (BM) is difficult. Macroautophagy/autophagy is critical for cancer initiation and progression. We hypothesized that genetic variants of autophagy-related genes may affect brain metastases (B...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2017.1308997
更新日期:2017-06-03 00:00:00
abstract::Macroautophagy/autophagy is emerging as an important process in adult muscle stem cells functions: it regulates metabolic reprogramming during activation from a quiescent state, maintains stemness and prevents senescence. We now show that autophagy is specifically required for neonatal myogenesis and muscle developmen...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2018.1507439
更新日期:2019-01-01 00:00:00
abstract::To maintain proper cellular homeostasis, the magnitude of autophagy activity has to be finely tuned in response to environmental changes. Many aspects of autophagy regulation have been extensively studied: pathways integrating signals through the master regulators TORC1 and PKA lead to multiple post-translational modi...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1018503
更新日期:2015-04-03 00:00:00
abstract::Retinal ischemia is a major cause of vision loss and a common underlying mechanism associated with diseases, such as diabetic retinopathy and central retinal artery occlusion. We have previously demonstrated the robust neuroprotection in retina induced by post-conditioning (post-C), a brief period of ischemia, 24 h, f...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1767371
更新日期:2020-05-26 00:00:00
abstract::Abnormal aggregation of SNCA/?-synuclein plays a crucial role in Parkinson disease (PD) pathogenesis. SNCA levels determine its toxicity, and its accumulation, even to a small extent, may be a risk factor for neurodegeneration. One of the main pathways for SNCA degradation is chaperone-mediated autophagy (CMA), a sele...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.26451
更新日期:2013-12-01 00:00:00
abstract::The European autophagy consortium Driving next-generation autophagy researchers towards translation (DRIVE) held its kick-off meeting in Groningen on the 14th and 15th of June 2018. This Marie Skłodowska-Curie Early Training Network was approved under the European Union's Horizon 2020 Research and Innovation Program a...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2018.1515532
更新日期:2019-02-01 00:00:00
abstract::Efavirenz (EFV) is the most widely used non-nucleoside reverse transcriptase inhibitor applied in highly active antiretroviral therapy (HAART), the combined pharmacological treatment of the human immunodeficiency virus infection. Its use has been associated with the development of several adverse events including hepa...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.7.11.17653
更新日期:2011-11-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of upper and lower motor neurons. As with other age-dependent neurodegenerative disorders, ALS is linked to the presence of misfolded proteins that may perturb several intracellular mechanisms and trigger neu...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6.7.13042
更新日期:2010-10-01 00:00:00
abstract::In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy in fusion-induced nuclear degradation. A search of the F...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/15548627.2014.994413
更新日期:2015-01-01 00:00:00
abstract::Degradation of intracellular components via macroautophagy is a complex multistep process that starts with the sequestration of cytosolic cargo in a de novo formed double-membrane vesicle or autophagosome. This compartment acquires the hydrolases required for cargo digestion by fusion with lysosomes. In contrast to th...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1096/fj.09-144519
更新日期:2010-08-01 00:00:00
abstract::Understanding of the mechanism for myeloid differentiation provides important insights into the hematopoietic developmental processes. By using an ESC-derived myeloid progenitor cell model, we found that CSF2/GM-CSF triggered macrophage differentiation and activation of the MTOR signaling pathway. Activation or inhibi...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2019.1578040
更新日期:2019-07-01 00:00:00
abstract::microRNAs (miRNAs) are a class of small regulatory RNAs that regulate gene expression at the post-transcriptional level. miRNAs play important roles in the regulation of development, growth, and metastasis of cancer, and in determining the response of tumor cells to anticancer therapy. In recent years, they have also ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.20053
更新日期:2012-05-01 00:00:00
abstract::Based on my reading, and on my own experience, I have come to realize that people learn in different ways, and this can include the use of different media. This is one reason I have worked with various artists to portray the topic of autophagy through paintings, music and dance. Indeed, comments from members of the au...
journal_title:Autophagy
pub_type: 评论,社论
doi:10.1080/15548627.2019.1630225
更新日期:2019-09-01 00:00:00
abstract::The best-characterized process of autophagy is macroautophagy. Many an article or talk has started with the phrase "...macroautophagy, hereafter referred to as autophagy." This one will be different because we are going to learn more about the person most responsible for increasing our understanding of chaperone-media...
journal_title:Autophagy
pub_type: 传,历史文章,杂志文章
doi:10.4161/auto.5.8.9476
更新日期:2009-11-01 00:00:00
abstract::ULK1 and ATG13 assemble with RB1CC1/FIP200 and ATG101 to form a macroautophagy (hereafter autophagy) induction (ULK1) complex in higher eukaryotes. The yeast counterpart, the Atg1 complex, is comprised of Atg1 and Atg13 (ULK1 and ATG13 homologs), Atg17 (a proposed functional homolog of RB1CC1), and either the Atg101 s...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1087635
更新日期:2015-11-02 00:00:00