Abstract:
:Hibernating mammals endure conditions of low body temperature and oxidative stress that would be highly injurious to humans and most other mammals. Stress conditions frequently trigger the production of molecular chaperones; in the endoplasmic reticulum the glucose-regulated protein-78 (GRP78) helps to minimize protein misfolding under stress. The present study evaluated the GRP78 response in seven organs of hibernating thirteen-lined ground squirrels, Spermophilus tridecemlineatus. Transcript levels of grp78, assessed by RT-PCR, were significantly higher (3.5- to 4.1-fold) in brown adipose tissue and brain of hibernating squirrels compared with euthermic control animals but remained low or stable in all other tissues. GRP78 protein content, assessed by Western blotting, was also elevated in brown adipose and brain during hibernation by 1.4-1.6 fold. A 2490 bp cDNA sequence was retrieved that contained the full length open reading frame of ground squirrel grp78 and the translated protein sequence of 654 amino acids shared 98-99% identity with GRP78 from other mammalian sources. Selected specific amino acid substitutions were found in the ground squirrel sequence that may aid GRP78 function under the near 0 degrees C body temperatures of the hibernating state. Electrophoretic mobility shift and supershift assays showed that the activating transcription factor, ATF4, binds to the promoter region of the grp78 gene in ground squirrel brain and may be responsible for grp78 up-regulation during hibernation. Changes in grp78 gene and protein expression appear to aid stress tolerance in two highly oxygen-dependent organs that are critical to whole animal survival during hibernation.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Mamady H,Storey KBdoi
10.1007/s11010-006-9221-8subject
Has Abstractpub_date
2006-11-01 00:00:00pages
89-98issue
1-2eissn
0300-8177issn
1573-4919journal_volume
292pub_type
杂志文章abstract::Administration of Ca2+ channel blockers in cardiac disorders and the central role of Ca2+ in modulating neutrophil functions, prompted us to investigate whether administration of nifedipine to mice would alter their natural resistance to infectious agents like Salmonella typhimurium. Neutrophil chemiluminescence (CL) ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00219971
更新日期:1990-06-25 00:00:00
abstract::Uterine leiomyoma (ULM) is the most common gynecological benign tumor that is affecting around 20-50 % of women over the age of 30. Although its molecular pathogenesis is still unknown, ULM has a multifactorial etiology determined by both genetics and environmental factors. The present study was designed to find out w...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-012-1540-3
更新日期:2013-03-01 00:00:00
abstract::Human papillomavirus (HPV) is considered to be a major etiological factor but is not sufficient for the development of cervical cancer. Other host factors including altered tumor suppressor gene activities might contribute to the carcinogenic process. Fragile Histidine Triad (FHIT) has been shown to play a pivotal rol...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-009-0240-0
更新日期:2010-02-01 00:00:00
abstract::In the present study, we investigated possible involvement of N-methyl-D-aspartate receptors (NMDAR) in estradiol mediated protection against ischemia reperfusion (I/R)-induced acute renal failure (ARF) in rats. Bilateral renal ischemia of 40 min followed by reperfusion for 24 h induced ARF in male wistar rats. Quanti...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-017-3034-9
更新日期:2017-10-01 00:00:00
abstract::The present review is focused on the main achievements realized in the lysozyme research field since the meeting held in 1972 to commemorate the fiftieth anniversary of the discovery of this enzyme. Despite of extensive structural, physico-chemical, crystallographic, genetic, immunological and evolutionary studies dev...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF00285225
更新日期:1984-09-01 00:00:00
abstract::1. Two different molecular forms of dopamine-beta-hydroxylase were isolated from human serum; a major component (Peak I enzyme) with a molecular weight of 368000 and with a higher specific activity and a minor component (Peak II enzyme) with a molecular weight of 188000 and with a lower specific activity. 2. Both form...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00280277
更新日期:1977-12-29 00:00:00
abstract::Long non-coding RNAs (lncRNAs) are known as crucial regulators in the development of OC. In the current study, we aim to explore the function and molecular mechanism of lncRNA DLEU1 in OC. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to determine the expression of DLEU1, miR-429, and TFAP2A i...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-020-03971-9
更新日期:2020-11-10 00:00:00
abstract::Hyperlipidemia is a well-known risk factor for atherosclerosis and statins are widely used to treat patients with elevated levels of lipids in their plasma. Notwithstanding the proven benefits of statin drugs on both primary and secondary prevention of heart disease, the high cost of statin treatment, in addition to p...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1017487815091
更新日期:2002-04-01 00:00:00
abstract::The (Ca2+ + Mg2+) ATPase which serves as a Ca2+ pump in the kidney basolateral membranes is essential to the maintenance of an intracellular Ca2+ concentration optimal for kidney function. Since atrial natriuretic peptide (ANP) is known to participate in the Ca2+ homeostasis mechanism, altered levels of ANP in diabete...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00230370
更新日期:1991-06-26 00:00:00
abstract::HMGb1 is a DNA-binding protein whose role as an extracellular cytokine in inflammation and tissue regeneration has also been reported. Given the importance of keratinocytes in wound healing, we have studied the mechanism of action of HMGb1 on HaCaT keratinocytes during in vitro scratch wound repair. Western blot and c...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-009-0192-4
更新日期:2009-12-01 00:00:00
abstract::Cardiovascular diseases involve critical mechanisms including impaired nitric oxide (NO) levels and abnormal matrix metalloproteinase (MMP) activity. While NO downregulates MMP expression in some cell types, no previous study has examined whether NO downregulates MMP levels in endothelial cells. We hypothesized that N...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-013-1602-1
更新日期:2013-06-01 00:00:00
abstract::The effects of Mg2+ concentration (Mg2+o, 0, 1.2, 2.4, and 4.8 mM) on the incidence of reperfusion arrhythmias and on the cellular electrical activity were studied in spontaneously beating rat hearts. The surface electrogram and the membrane potential were recorded in control conditions, during 10 min of regional isch...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1006863311967
更新日期:1997-06-01 00:00:00
abstract::Krüppel-like factor 4 (KLF4), a zinc finger transcription factor, has been implicated in the inflammation mediated by macrophages and endothelial cells by regulating the expression of inflammatory mediators. Here, we investigated whether KLF4 affects the expression of inducible nitric oxide synthase (iNOS), an importa...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-017-3115-9
更新日期:2018-01-01 00:00:00
abstract::Both idiopathic restrictive cardiomyopathy (IRCM) and hypertrophic cardiomyopathy (HCM) are part of the same disease spectrum and are due to sarcomeric gene mutations. A patient with restrictive physiology without left ventricular hypertrophy (LVH) would be diagnosed as IRCM, while one with LVH would be diagnosed as H...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-009-0157-7
更新日期:2009-11-01 00:00:00
abstract::Adipose-derived stem cells (ADSCs) have been considered as the optimal cells for regenerative medicine because ADSCs have the potential of multi-directional differentiation. To study the mechanisms of ADSCs differentiation, we analyzed microarray of GSE37329. GSE37329 was downloaded from Gene Expression Omnibus includ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,收录出版
doi:10.1007/s11010-014-2269-y
更新日期:2015-02-01 00:00:00
abstract::The expression of 76 sequences, previously isolated as differentially expressed in visceral white adipose tissue (WAT) of female rats, fed with a high-fat diet for 11 days (Lopez et al., Biochem Biophys Res Comm 318: 234-239, 2004), was analyzed in epidydimal WAT of male rats after a feeding period of 65 days with the...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-1082-z
更新日期:2005-07-01 00:00:00
abstract::Ciprofloxacin induced an increment of reactive oxygen species in sensitive strains of Staphylococcus aureus leading to oxidative stress detected by chemiluminescence while resistant strains did not suffer such stress. Oxidation of lipids was performed by employing thiobarbituric acid reaction to detect the formation o...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-9051-0
更新日期:2006-04-01 00:00:00
abstract::Cardiosphere-derived cells (CDCs) and bone marrow mesenchymal stem cells (MSCs) are popularly used in stem cell therapy for myocardial regeneration. The cell type that survives and maintains stem cell characteristics in the adverse microenvironment following ischemia-reperfusion injury is presumed to be ideal for tran...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-016-2869-9
更新日期:2017-01-01 00:00:00
abstract::Logistic complexities of heart transplantation embossed the necessity of utilizing novel methods, which enable heart regeneration. Human cardiosphere-derived cells (hCDCs) are taken into consideration as a promising cell resource in cell therapy in recent years. In this study, we designed an electrochemical stimulatio...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-020-03742-6
更新日期:2020-07-01 00:00:00
abstract::Human and bovine nasal proteoglycan complex components were prepared in parallel. Some molecular properties of human and bovine proteoglycan subunits (PGS) were compared. Two major human "link-like proteins" have been characterized and purified; their compositions and molecular weights were very similar to those obser...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00240278
更新日期:1978-11-01 00:00:00
abstract::Diabetes mellitus is a serious global health problem, and cardiovascular complications are the major cause of morbidity and mortality in diabetic patients. The chronic effects of neonatal alloxan- (ALX) induced diabetes mellitus on ventricular myocyte contraction and intracellular Ca(2+) transport have been investigat...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-010-0613-4
更新日期:2011-01-01 00:00:00
abstract::In mammalian cells, increases in calcium concentration cause increases in oxidative phosphorylation. This effect is mediated by the activation of four mitochondrial dehydrogenases by calcium ions; FAD-glycerol 3-phosphate dehydrogenase, pyruvate dehydrogenase, NAD-isocitrate dehydrogenase and oxoglutarate dehydrogenas...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01076578
更新日期:1995-08-01 00:00:00
abstract::Concentrations of 1-4 mumol l-1 isoproterenol cause both in right ventricular papillary muscles and in enzymatically isolated myocytes of the guinea-pig a Ca2+ overload-induced state which is functionally characterized by biphasic (multiphasic) twitches and biphasic (multiphasic) intracellular calcium transients, resp...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00240026
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:We reported that urinary L-FABP reflected the progression of chronic kidney disease (CKD). This study is aimed to evaluate the clinical significance of urinary liver type fatty acid binding protein (L-FABP) as a biomarker for monitoring CKD. METHODS:Urinary L-FABP was measured using human L-FABP ELISA kit (...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-9047-9
更新日期:2006-03-01 00:00:00
abstract::The antioxidant activity of anthocyanins has been well characterized in vitro; many cases has been postulated to provide an important exogenous mediator of oxidative stress in the gastrointestinal tract. The objective of this study was to evaluate the efficacy of anthocyanin protection against peroxyl radical (AAPH)-i...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-008-9729-1
更新日期:2008-05-01 00:00:00
abstract::The oxygenase domain of the inducible nitric oxide synthase, Delta65 iNOSox is a dimer that binds heme, L-Arginine (L-Arg), and tetrahydrobiopterin (H(4)B) and is the site for NO synthesis. The role of H(4)B in iNOS structure-function is complex and its exact structural role is presently unknown. The present paper pro...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-9027-0
更新日期:2006-03-01 00:00:00
abstract::Biochemical and structural changes occurring in the myocardium with aging are mainly resulting from the association of a general tissue atrophy with the hypertrophy of the remaining myocytes. Whilst hypertrophy seems to be a compensatory process to the loss of cardiomyocytes and to a mild systolic hypertensive conditi...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF00240046
更新日期:1996-07-01 00:00:00
abstract::Recent reports considered that it was the disturbance of calcium homeostasis and the accumulation of misfolded proteins in the endoplasmic reticulum (ER) that activated hepatic stellate cells (HSCs) apoptosis and promoted fibrosis resolution. However, the signal-transducing events that are activated by ER stress after...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-014-2073-8
更新日期:2014-09-01 00:00:00
abstract::This study examined the effect of melatonin (MLT) on in vitro phagocytosis of testicular macrophages taken from control and streptozotocin (STZ)-induced diabetic rats and the possible mechanism of its action. The phagocytic activity was measured as a number of latex beads ingested by 100 macrophages (PI, phagocytic in...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-1995-6
更新日期:2005-07-01 00:00:00
abstract::A preparation of UDP-GalNAc:sialosyl lactosylceramide N-acetylgalactosaminyl transferase (E.C. 2.4.1.92) obtained from 14-day chick embryo brain was delipidated partially by treatment with cold acetone in the presence of varying amounts of sodium dodecyl sulphate (SDS). The lipid content and the enzyme activity of the...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00223509
更新日期:1989-01-23 00:00:00