Abstract:
BACKGROUND:All current major medications in treating endometriosis are effective in treating pain, most likely through suppression of proliferation of the implants, yet their effectiveness is relatively short term and they all have many undesirable, and sometimes severe, side effects. There is pressing need for novel, more effective medications in treating endometriosis with less and/or milder side effects. METHODS:Using a recently established immortalized endometrial stromal cell line, we carried out cell proliferation assays for cells treated with trichostatin A (TSA), RU486, CDB-2914, and N-acetylcysteine, and ICI 182780. Gene expression levels for PR-A, PR-B, AR, Fas and FasL were measured. Protein expression levels for ERalpha, ERbeta, and AR were also measured. RESULTS:Cell proliferation assay results for NAC, H2O2, CDB, and RU486 were nearly identical or similar to what have been reported based on primary cell cultures or in vivo studies. TSA, CDB, RU486 and NAC all had various antiproliferative effects. TSA had a more potent and longer lasting antiproliferative effect than CDB and NAC, even in the presence of an oxidant, H2O2. Its antiproliferative effect was concentration-dependent. ICI did not have a significant antiproliferative effect. PR-A, PR-B, AR, and FasL expression were all increased as compared with untreated cells. CONCLUSIONS:The cell line appears to be an adequate model for stromal components of endometriotic implants. That ICI has no inhibitory effect on endometrial proliferation may explain why a phase II clinical trial on its use to treat endometriosis did not advance to later stages. The upregulation of PR-B and AR may be responsible for antiproliferative effects induced by TSA, a histone deacetylase inhibitor (HDACI). HDACIs may be promising therapeutics in treating endometriosis due to their antiproliferative effects as well as the potential to restore gene dysregulation through chromatin remodeling.
journal_name
Gynecol Obstet Investjournal_title
Gynecologic and obstetric investigationauthors
Wu Y,Guo SWdoi
10.1159/000093975subject
Has Abstractpub_date
2006-01-01 00:00:00pages
193-205issue
4eissn
0378-7346issn
1423-002Xpii
93975journal_volume
62pub_type
杂志文章abstract::The aim of this study was to present our preliminary data about nasal bone measurements at 11-14 weeks of pregnancy. This study was conducted in our prenatal unit between 2000 and 2003. A total of 642 pregnant women (single pregnancies) were enrolled into the study. During the first trimester of pregnancy, crown-rump ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000078499
更新日期:2004-01-01 00:00:00
abstract::Fetal echocardiographic and Doppler ultrasonographic prospective studies were performed in utero on 299 babies delivered at Hirata Municipal Hospital, Shimane, Japan, from May 1984 to June 1986. Two or three ultrasonographic examinations were performed on each fetus from 20 weeks of gestation to term. Three congenital...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293682
更新日期:1988-01-01 00:00:00
abstract::Implantation of intrauterine devices (IUDs) is associated with an increased incidence of uterine infection, probably as a result of vaginal bacteria entering the uterus at the time of insertion. To reduce the incidence of IUD-related infections, the incorporation of antimicrobial agents into the tail of the device was...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010256
更新日期:2000-01-01 00:00:00
abstract::The 784 cases, from which 701 complained of abnormal uterine bleeding, were reviewed by age groups, presenting symptoms, sex steroid medication and endometrial findings. Endometrial polyps and endometritis, which showed an incidence of 17.5 and 10.0%, respectively, were correlated with clinicopathologic findings. Peri...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299980
更新日期:1979-01-01 00:00:00
abstract:HYPOTHESIS:The new hysteroscopic system with mechanical energy is an effective outpatient technique for diagnosis and treatment that has certain advantages over conventional hysteroscopy in the management of endometrial polyps. OBJECTIVES:Our primary objective was to assess the total duration of hysteroscopy and polyp...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,随机对照试验
doi:10.1159/000377700
更新日期:2015-01-01 00:00:00
abstract::Progesterone secretion of cultured human granulosa cells treated with LHRH or its analogues was measured. Granulosa cells were also stimulated with LH, FSH, cAMP or forskolin. No differences could be observed in the progesterone basal secretion of cultures treated with or without LHRH or its analogues and after the va...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292765
更新日期:1992-01-01 00:00:00
abstract::Pre-implantation blastocysts or trophoblast, obtained from ectoplacental cones, were transferred to the right oviducts of both pregnant and nonpregnant mice. Blastocysts which remained within the oviductal lumen did not show evidence of further differentiation into trophoblast although they readily implanted on variou...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299117
更新日期:1984-01-01 00:00:00
abstract::The patterns of plasma melatonin, gonadotropins, sex steroids and prolactin were studied in anovulatory infertile females undergoing ovulation induction with hMG/hCG. Melatonin levels were found to fluctuate during the menstrual cycle of these subjects with a nadir at mid-cycle and peak occurring at the early follicul...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000009977
更新日期:1998-01-01 00:00:00
abstract::Serum profiles of growth hormone (GH) were recorded for 24 h in 9 young healthy women before and during treatment with monophasic, combined contraceptives. During treatment, there was a change in GH secretion towards a pattern of smaller peaks at a higher frequency. The mean peak amplitude decreased from 11.2 to 7.3 m...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293276
更新日期:1990-01-01 00:00:00
abstract::The cardiovascular and metabolic responses during acute fetal hypoxia were studied in 7 pregnant sheep near term. Complete reduction of uterine flow (UBF) for 180 s was performed in 13 experiments by inflating a balloon positioned in the maternal aorta. Fetal heart rate (FHR), systolic and diastolic blood pressure (BP...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299425
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:To clarify the alteration of maternal-fetal mixed lymphocyte reaction (MLR) in intrahepatic cholestasis of pregnancy (ICP). METHODS:Twenty-two patients with intrahepatic cholestasis of pregnancy and 21 normal pregnant women were recruited into the present study. Of patients with ICP, 5 also experienced pregn...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000068381
更新日期:2002-01-01 00:00:00
abstract::Scanning electron microscopy of cryofractured immature intermediate villi showed numerous intercommunicating channels in the central region of the stroma, oriented mostly parallel to the major axis of the villus. In the stromal channels, numerous Hofbauer cells were present and their aspect and position inside the cha...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299056
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND/AIMS:To determine the clinicopathological characteristics and potentially associated outcomes in patients diagnosed with serous adenocarcinoma of the uterine cervix. METHODS:The records of surgically-treated patients with pathological stage pT1b-2b serous adenocarcinoma were reviewed. RESULTS:Of 12 patient...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,评审
doi:10.1159/000329319
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND/AIMS:Limited information is available regarding the quantity of blood loss associated with uncomplicated transvaginal oocyte retrieval. The aim of the present study was evaluating the quantity of such a loss. METHODS:One hundred and fifty consecutive women undergoing oocyte retrieval were recruited. They un...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000158649
更新日期:2009-01-01 00:00:00
abstract::A dietary approach has proven to be effective in alleviating symptoms of premenstrual syndrome. In our previous studies, magnesium improved premenstrual irritability and mood scoings. In this double-blind, placebo-controlled study, we evaluated the effects of a new dietetic preparation (Sillix Donna, Giuliani) in 40 p...
journal_title:Gynecologic and obstetric investigation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000291836
更新日期:1997-01-01 00:00:00
abstract::An evaluation of the age distribution of erythrocytes in twin gestation was performed. Based on previous studies on age distribution of erythrocytes in late pregnancy, we have proposed that a more pronounced shift in age distribution of erythrocytes in twin pregnancy may be expected. We have used the density distribut...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292618
更新日期:1993-01-01 00:00:00
abstract::The potential risk of rhesus alloimmunization and the ensuing risk of fetal death with increasing parity were investigated in two groups of parturients; primiparous and grand multiparous (para > or = 5) women with liveborns. It was hypothesized that significantly fewer women of the latter than of the former group woul...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292744
更新日期:1992-01-01 00:00:00
abstract::Pelvic masses can be diagnosed by pelvic ultrasonography. Difficulties may be encountered to identify the organ origin of a pelvic mass. An intrauterine marker is proposed to facilitate the diagnosis by the exact localization of the uterus and its relationship to other pelvic findings. ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000298777
更新日期:1987-01-01 00:00:00
abstract::The theory that the Th1 and Th2 cell ratio shifts towards Th2 dominance during pregnancy may improve fetal survival has gained support from recent studies. Also, the variation in the Th1/Th2 cell ratio is reportedly associated with intrauterine growth retardation and preeclampsia. On the basis of these findings, the a...
journal_title:Gynecologic and obstetric investigation
pub_type: 临床试验,杂志文章
doi:10.1159/000010328
更新日期:2000-01-01 00:00:00
abstract:AIM:This study aimed to clarify the genetic and epigenetic features of recurrent hydatidiform mole (RHM) in Japanese patients. METHODS:Four Japanese isolated RHM cases were analyzed using whole-exome sequencing. Villi from RHMs were collected by laser microdissection for genotyping and DNA methylation assay of differe...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000441780
更新日期:2016-01-01 00:00:00
abstract::We report an extremely rare case of a 50-year-old woman with an adenocarcinoma arising within a urethral diverticulum. She could not be diagnosed correctly before operation and the microscopic pathological examination of the surgical specimen was the only indication showing the cancer origin. ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292307
更新日期:1995-01-01 00:00:00
abstract::Haptoglobin (Hp) possesses many functions in the immune system and angiogenesis as well as scavenging released hemoglobin, and thus it is speculated that Hp may also play a critical role in the reproductive process. To investigate the possible involvement of Hp in the outcome of in vitro fertilization and embryo trans...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000058053
更新日期:2001-01-01 00:00:00
abstract::As part of a case control within cohort study 472 pregnant women answered a questionnaire post partum. Serum relaxin concentrations were measured by a homologous ELISA in samples collected from the women in the 30th week of pregnancy. Serum relaxin concentrations were not associated with pregnancy-associated pelvic pa...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292438
更新日期:1994-01-01 00:00:00
abstract:OBJECTIVE:To determine whether a diminished intrapartum amniotic fluid volume represents a risk of adverse neonatal outcome when it occurs in conjunction with prolonged labor. METHODS:The intrapartum amniotic fluid index (iAFI) was measured in 242 parturients over 35 weeks of gestation during 1st-stage labor, and the ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000049402
更新日期:2002-01-01 00:00:00
abstract::In 25 pregnant females with a placental weight below the 20th percentile uteroplacental blood flow measurements using 113mIn-transferrin were performed in the 3rd trimester of pregnancy. Twelve patients with pregnancy-induced hypertension (PIH) were compared to 13 females with normal blood pressures. The results of ut...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000298931
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:Immunological deficiencies, altered angiogenic activity, infiltrative potential and growth factors are plausible factors behind endometriosis. The aim of this study was to determine whether endometriosis interferes with the course or outcome of pregnancy. STUDY DESIGN:In this matched case-control study, we ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000074815
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:To study the association of umbilical cord presentation found on antenatal ultrasound and the incidence of cord prolapse in labor. STUDY DESIGN:We reviewed the antenatal records of all deliveries in the Mount Sinai Hospital in a 5-year period and conducted two separate retrospective studies. In the first stu...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000072323
更新日期:2003-01-01 00:00:00
abstract::A 29-year-old woman presented with preterm labour at 32 weeks of gestation. Tocolytic treatment was started with intravenous hexoprenaline. Twenty-four hours after initiation of treatment, the patient developed supraventricular tachycardia, resistant to digoxin and verapamil. Medical treatment with metoprolol finally ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292411
更新日期:1995-01-01 00:00:00
abstract::Immunochemical methods were used to identify neuropeptide Y (NPY) in the cervical tissue of women at early and term pregnancy. NPY-containing fibers could not be demonstrated in the upper and lower uterine segments at term, but the cervical innervation persisted during labor. Moreover, NPY alone did not affect cervica...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292754
更新日期:1992-01-01 00:00:00
abstract::Gestational diabetes (GD), defined as carbohydrate intolerance with onset or first recognition during pregnancy, has a prevalence of 7% and is a growing problem worldwide. Infants born to mothers with GD are more likely to be large for gestational age, incur traumatic birth injury, require a stay in the intensive care...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,评审
doi:10.1159/000357616
更新日期:2014-01-01 00:00:00