Isolation and characterization of virus-specific double-stranded DNA from tissues infected by bean golden mosaic virus.

abstract：:Swainsonine, an indolizidine alkaloid, inhibits the processing of asparagine-linked glycoproteins in both cell-free extracts and animal cells in culture. Thus, in a liver particulate enzyme preparation, swainsonine at 0.1-1.0 microM inhibited the mannosidase that releases [3H]mannose from a high mannose glycopeptide b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elbein AD,Solf R,Dorling PR,Vosbeck K

更新日期：1981-12-01 00:00:00

abstract：:The biodistribution profiles in mice of two pyrrole-imidazole polyamides were determined by PET. Pyrrole-imidazole polyamides are a class of small molecules that can be programmed to bind a broad repertoire of DNA sequences, disrupt transcription factor-DNA interfaces, and modulate gene expression pathways in cell cul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harki DA,Satyamurthy N,Stout DB,Phelps ME,Dervan PB

更新日期：2008-09-02 00:00:00

abstract：:We used a model system of purified components to explore the effects of a downstream target on the signaling properties of a covalent modification cycle, an example of retroactivity. In the experimental system used, a bifunctional enzyme catalyzed the modification and demodification of its substrate protein, with both...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ventura AC,Jiang P,Van Wassenhove L,Del Vecchio D,Merajver SD,Ninfa AJ

更新日期：2010-06-01 00:00:00

abstract：:The morbidity and mortality associated with Vibrio-mediated waterborne diseases necessitates the development of sensitive detection technologies that are able to elucidate the identity, potential pathogenicity, susceptibility, and viability of contaminating bacteria in a timely manner. For this purpose, we have design...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vora GJ,Meador CE,Bird MM,Bopp CA,Andreadis JD,Stenger DA

更新日期：2005-12-27 00:00:00

abstract：:Focal cerebral ischemia and traumatic brain injury induce an escalating amount of cell death because of harmful mediators diffusing from the original lesion site. Evidence suggests that healthy cells surrounding these lesions attempt to protect themselves by producing endocannabinoids (eCBs) and activating cannabinoid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Witting A,Chen L,Cudaback E,Straiker A,Walter L,Rickman B,Möller T,Brosnan C,Stella N

更新日期：2006-04-18 00:00:00

abstract：:The IL-2R gamma chain (IL-2R gamma) is an essential component of high- and intermediate-affinity IL-2Rs, playing critical roles for ligand binding and internalization. Recently, our laboratory has demonstrated that IL-2R gamma mutation results in X chromosome-linked severe combined immunodeficiency in humans, suggesti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cao X,Kozak CA,Liu YJ,Noguchi M,O'Connell E,Leonard WJ

更新日期：1993-09-15 00:00:00

abstract：:Developing autoreactive B cells edit their B cell antigen receptor (BCR) in the bone marrow and are clonally deleted when they fail to reexpress an innocent BCR. Here, inducible Cre-loxP-mediated gene inversion is used to change the specificity of the BCR on mature IgM+ IgD+ B cells in vivo to address the fate of lymp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lam KP,Rajewsky K

更新日期：1998-10-27 00:00:00

abstract：:Heat shock (HS) initiates rapid, extensive, and evolutionarily conserved changes in transcription that are accompanied by chromatin decondensation and nucleosome loss at HS loci. Here we have employed in situ Hi-C to determine how heat stress affects long-range chromatin conformation in human and Drosophila cells. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ray J,Munn PR,Vihervaara A,Lewis JJ,Ozer A,Danko CG,Lis JT

更新日期：2019-09-24 00:00:00

abstract：:Obesity is associated with increased risk for developing pancreatic cancer, and it is suggested that insulin resistance provides the missing link. Here we demonstrate that under the context of genetic susceptibility, a high fat diet (HFD) predisposes mice with oncogenic K-ras activation to accelerated pancreatic intra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khasawneh J,Schulz MD,Walch A,Rozman J,Hrabe de Angelis M,Klingenspor M,Buck A,Schwaiger M,Saur D,Schmid RM,Klöppel G,Sipos B,Greten FR,Arkan MC

更新日期：2009-03-03 00:00:00

abstract：:Linear double-stranded molecules of the circularly permuted and terminally redundant DNA of Salmonella bacteriophage P22 have been converted to oligomeric products in the presence of polynucleotide ligase coded for by the coliphage T4. The reaction has been monitored by sucrose density-gradient centrifugation and elec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sgaramella V

更新日期：1972-11-01 00:00:00

abstract：:The D1 reaction center protein of photosystem II (PSII) is subject to light-induced damage. Degradation of damaged D1 and its replacement by nascent D1 are at the heart of a PSII repair cycle, without which photosynthesis is inhibited. In mature plant chloroplasts, light stimulates the recruitment of ribosomes specifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chotewutmontri P,Barkan A

更新日期：2020-09-01 00:00:00

abstract：:Layer 5 pyramidal neurons process information from multiple cortical layers to provide a major output of cortex. Because of technical limitations it has remained unclear how these cells integrate widespread synaptic inputs located in distantly separated basal and tuft dendrites. Here, we obtained in vivo two-photon ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill DN,Varga Z,Jia H,Sakmann B,Konnerth A

更新日期：2013-08-13 00:00:00

abstract：:Scientific and engineering innovation is vital for American competitiveness, quality of life, and national security. However, too few American students, especially women, pursue these fields. Although this problem has attracted enormous attention, rigorously tested interventions outside artificial laboratory settings ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dennehy TC,Dasgupta N

更新日期：2017-06-06 00:00:00

abstract：:Dendritic spines receive most synaptic inputs in the forebrain. Their morphology, with a spine head isolated from the dendrite by a slender neck, indicates a potential role in isolating inputs. Indeed, biochemical compartmentalization occurs at spine heads because of the diffusional bottleneck created by the spine nec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Araya R,Jiang J,Eisenthal KB,Yuste R

更新日期：2006-11-21 00:00:00

abstract：:The ability of the radiomimetic anticancer enediyne C-1027 to induce ataxia-telangiectasia mutated (ATM) and ATM and Rad3-related (ATR)-independent damage responses was discovered to reside in its unique ability to concurrently generate robust amounts of double-strand breaks (DSBs) and interstrand cross-links (ICLs) i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kennedy DR,Ju J,Shen B,Beerman TA

更新日期：2007-11-06 00:00:00

abstract：:Alternative adaptations-different adaptive phenotypes maintained in the same life stage and the same population but not necessarily simultaneously expressed in the same individual-represent contrasting character sets produced by the same genome, in effect allowing a single species to occupy more than one sympatric nic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： West-Eberhard MJ

更新日期：1986-03-01 00:00:00

abstract：:Although heparin and low-molecular-weight heparins (LMWH) have been widely used clinically as anticoagulants, their broader use has been limited by the lack of noninvasive delivery methods for this class of molecules. In this study, we demonstrate an efficient, rapid, and reproducible delivery system for heparin throu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qi Y,Zhao G,Liu D,Shriver Z,Sundaram M,Sengupta S,Venkataraman G,Langer R,Sasisekharan R

更新日期：2004-06-29 00:00:00

abstract：:Water freezing on solid surfaces is ubiquitous in nature. Even though icing/frosting impairs the performance and safety in many processes, its mechanism remains inadequately understood. Changing atmospheric conditions, surface properties, the complexity of icing physics, and the unorthodox behavior of water are the pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jung S,Tiwari MK,Poulikakos D

更新日期：2012-10-02 00:00:00

abstract：:Anthrax toxin is an intracellularly acting toxin in which sufficient information is available regarding the structure of its transmembrane channel, allowing for detailed investigation of models of translocation. Anthrax toxin, comprising three proteins-protective antigen (PA), lethal factor (LF), and edema factor-tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Das D,Krantz BA

更新日期：2016-08-23 00:00:00

abstract：:Myelin basic proteins (MBP) are major constituents of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. We previously showed that MBP-related transcripts are present in the bone marrow and the immune system. These mRNAs are transcribe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marty MC,Alliot F,Rutin J,Fritz R,Trisler D,Pessac B

更新日期：2002-06-25 00:00:00

abstract：:The latent membrane protein (LMP1) of Epstein-Barr virus (EBV) is expressed in EBV-associated nasopharyngeal carcinoma, which is notoriously metastatic. Although it is established that LMP1 represses E-cadherin expression and enhances the invasive ability of carcinoma cells, the mechanism underlying this repression re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai CN,Tsai CL,Tse KP,Chang HY,Chang YS

更新日期：2002-07-23 00:00:00

abstract：:We have identified a protein present only in erythroid cells that binds to two adjacent sites within an enhancer region of the chicken beta-globin locus. Mutation of the sites, so that binding by the factor can no longer be detected in vitro, leads to a loss of enhancing ability, assayed by transient expression in pri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Evans T,Reitman M,Felsenfeld G

更新日期：1988-08-01 00:00:00

abstract：:The human isoform 2 of pantothenate kinase (PanK2) is localized to the mitochondria, and mutations in this protein are associated with a progressive neurodegenerative disorder. PanK2 inhibition by acetyl-CoA is so stringent (IC50 < 1 microM) that it is unclear how the enzyme functions in the presence of intracellular ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leonardi R,Rock CO,Jackowski S,Zhang YM

更新日期：2007-01-30 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus 2 originated in Wuhan, China at the end of 2019 and rapidly spread in more than 100 countries. Researchers in different fields have been working on finding explanations for the unequal impact of the virus and deaths from the associated coronavirus disease 2019 (COVID-1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arpino B,Bordone V,Pasqualini M

更新日期：2020-08-11 00:00:00

abstract：:Adult workers in honeybee (Apis mellifera) colonies exhibit plasticity in hormonally regulated, age-based division of labor by altering their pattern of behavioral development in response to changes in colony conditions. One form of this plasticity is precocious development: levels of juvenile hormone increase prematu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang ZY,Robinson GE

更新日期：1992-12-15 00:00:00

abstract：:Recombinant human migration inhibitory factor (MIF), isolated through functional expression cloning in COS-1 cells, up-regulates expression of genes encoding HLA-DR and interleukin 1 beta (IL-1 beta) and elaboration of IL-1 beta by human monocyte-derived macrophages. Administration of soluble bovine serum albumin or h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiser WY,Pozzi LM,Titus RG,David JR

更新日期：1992-09-01 00:00:00

abstract：:Fas, a member of the tumor necrosis factor receptor family, can induce apoptosis when activated by Fas ligand binding or anti-Fas antibody crosslinking. Genetic studies have shown that a defect in Fas-mediated apoptosis resulted in abnormal development and function of the immune system in mice. A point mutation in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu K,Niu X,Williams LT

更新日期：1995-12-05 00:00:00

abstract：:We determined the complete nucleotide sequence of an infectious proviral molecular clone (FIV-14) of the feline immunodeficiency virus (FIV). FIV-14 has a genome organization similar in complexity to other lentiviruses. In addition to three large open reading frames representing the gag, pol, and env genes, at least f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olmsted RA,Hirsch VM,Purcell RH,Johnson PR

更新日期：1989-10-01 00:00:00

abstract：:Vision loss affects >3 million Americans and many more people worldwide. Although predisposing genes have been identified their link to known environmental factors is unclear. In wild-type animals DNA alkylating agents induce photoreceptor apoptosis and severe retinal degeneration. Alkylation-induced retinal degenerat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meira LB,Moroski-Erkul CA,Green SL,Calvo JA,Bronson RT,Shah D,Samson LD

更新日期：2009-01-20 00:00:00

abstract：:A major challenge facing the genetics of autism spectrum disorders (ASDs) is the large and growing number of candidate risk genes and gene variants of unknown functional significance. Here, we used Caenorhabditis elegans to systematically functionally characterize ASD-associated genes in vivo. Using our custom machine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McDiarmid TA,Belmadani M,Liang J,Meili F,Mathews EA,Mullen GP,Hendi A,Wong WR,Rand JB,Mizumoto K,Haas K,Pavlidis P,Rankin CH

更新日期：2020-01-07 00:00:00