High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes.

abstract：:Certain mutations at the glaB locus result in the failure to fully derepress glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] and to convert it to the active nonadenylylated form in response to nitrogen limitation. In these mutants the PII regulatory protein is altered such that it cannot be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Foor F,Reuveny Z,Magasanik B

更新日期：1980-05-01 00:00:00

abstract：:Auditory speech perception enables listeners to access phonological categories from speech sounds. During speech production and speech motor learning, speakers' experience matched auditory and somatosensory input. Accordingly, access to phonetic units might also be provided by somatosensory information. The present st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patri JF,Ostry DJ,Diard J,Schwartz JL,Trudeau-Fisette P,Savariaux C,Perrier P

更新日期：2020-03-17 00:00:00

abstract：:Interleukin 10 (IL-10) inhibits interferon gamma-induced macrophage activation for cytotoxicity against larvae of the human parasite Schistosoma mansoni by suppressing production of the toxic effector molecule nitric oxide (NO). In this study, the mechanism of IL-10 action was identified as inhibition of endogenous tu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oswald IP,Wynn TA,Sher A,James SL

更新日期：1992-09-15 00:00:00

abstract：:The ever-increasing industrial demand for nylon-6 (polycaprolactam) necessitates the development of environmentally benign methods of producing its precursor, epsilon-caprolactam, from cyclohexanone. It is currently manufactured in two popular double-step processes, each of which uses highly aggressive reagents, and e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas JM,Raja R

更新日期：2005-09-27 00:00:00

abstract：:Despite the enormous theoretical attention given to the evolutionary consequences of sexual reproduction, the validity of the key assumptions on which the theory depends rarely has been evaluated. It is often argued that a reduced ability to purge deleterious mutations condemns asexual lineages to an early extinction....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Omilian AR,Cristescu ME,Dudycha JL,Lynch M

更新日期：2006-12-05 00:00:00

abstract：:A human metallothionein (MT) gene was inserted into a bovine papillomavirus (BPV) vector. The chimeric vector (pMTII-BPV) transforms rodent fibroblasts to a cadmium-resistant phenotype. The resistance is due to the high level of expression of human MT-II in those cells. The vector is maintained in the cells as a free ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karin M,Cathala G,Nguyen-Huu MC

更新日期：1983-07-01 00:00:00

abstract：:The C terminus of transcription factor NusA from Escherichia coli comprises two repeat units, which bind during antitermination to protein N from phage lambda. To delineate the structural basis of the NusA-lambdaN interaction, we attempted to crystallize the NusA C-terminal repeats in complex with a lambdaN peptide (r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonin I,Mühlberger R,Bourenkov GP,Huber R,Bacher A,Richter G,Wahl MC

更新日期：2004-09-21 00:00:00

abstract：:Proceedings of the National Academy of Sciences Colloquium on the roles of homologous recombination in DNA replication are summarized. Current findings in experimental systems ranging from bacteriophages to mammalian cell lines substantiate the idea that homologous recombination is a system supporting DNA replication ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kuzminov A

更新日期：2001-07-17 00:00:00

abstract：:Sonic hedgehog (Shh) signaling plays diverse roles during animal development and adult tissue homeostasis through differential regulation of Gli family transcription factors. Dysregulated Shh signaling activities have been linked to birth defects and tumorigenesis. Here we report that Brg, an ATP-dependent chromatin r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhan X,Shi X,Zhang Z,Chen Y,Wu JI

更新日期：2011-08-02 00:00:00

abstract：:Systemic delivery of bioactive molecules in the CNS is hampered by the blood-brain barrier, which has bottlenecked noninvasive physiological study of the brain and the development of CNS drugs. Here we report that irradiation with an ultrashort pulsed laser to the blood vessel wall induces transient leakage of blood p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi M,Ku T,Chong K,Yoon J,Choi C

更新日期：2011-05-31 00:00:00

abstract：:Escherichia coli lipopolysaccharide (LPS)-induced nitrate biosynthesis was studied in LPS-sensitive C3H/He and LPS-resistant C3H/HeJ mice. Intraperitoneal injection of 15 micrograms of LPS led to a temporary 5- to 6-fold increase in blood nitrate concentration in the C3H/He strain. Levels of nitrate excreted in the ur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stuehr DJ,Marletta MA

更新日期：1985-11-01 00:00:00

abstract：:The integrin receptor alphavbeta5 controls two independent forms of interactions of the retinal pigment epithelium (RPE) with adjacent photoreceptor outer segments that are essential for vision. Alphavbeta5 localizes specifically to apical microvilli of the RPE and contributes to retinal adhesion that maintains RPE co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nandrot EF,Anand M,Almeida D,Atabai K,Sheppard D,Finnemann SC

更新日期：2007-07-17 00:00:00

abstract：:The rate of Cl- secretion by human airway epithelium is determined, in part, by apical cell membrane Cl- conductance. In cystic fibrosis airway epithelia, defective regulation of Cl- conductance decreases the capability to secrete Cl-. Here we report that extracytosolic ATP in the luminal bath of cultured human airway...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stutts MJ,Chinet TC,Mason SJ,Fullton JM,Clarke LL,Boucher RC

更新日期：1992-03-01 00:00:00

abstract：:Accumulation of misfolded proteins in the endoplasmic reticulum (ER) triggers the unfolded protein response (UPR), an intracellular signaling pathway that adjusts the protein folding capacity of the ER according to need. If homeostasis in the ER protein folding environment cannot be reestablished, cells commit to apop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,Korennykh AV,Behrman SL,Walter P

更新日期：2010-09-14 00:00:00

abstract：:High molecular weight RNA (35S) isolated from avian myeloblastosis virus directs the cell-free synthesis of two prominent polypeptides of 180,000 and 76,000 molecular weight. The latter polypeptide has previously been identified as the precursor to the group-specific antigens of the virus ("gag" proteins) [Vogt, V. M....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paterson BM,Marciani DJ,Papas TS

更新日期：1977-11-01 00:00:00

abstract：:Adrenocorticotropic hormone (ACTH) inhibited [3H]thymidine incorporation in normal adrenocortical cells of adult rats in culture, with a concomitant increase in corticosterone production and a characteristic retraction of cells. Both dibutyryl cyclic AMP and an analog of ACTH, which produces virtually no cyclic AMP, i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramachandran J,Suyama AT

更新日期：1975-01-01 00:00:00

abstract：:We have detected a proteolytic mechanism in chloroplasts that selectively and rapidly degrades the imported small subunit of ribulose 1,5-bisphosphate carboxylase when pools of the chloroplast-synthesized large subunit are depleted. This degradation system is constitutively present and appears to be responsible for pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmidt GW,Mishkind ML

更新日期：1983-05-01 00:00:00

abstract：:Recently, surface-sensitive spectroscopy data and molecular dynamics simulations have generated intense interest in the distribution of electrolyte ions between bulk water and the air/water interface. A partitioning model for cations and anions developed for biopolymer surface is extended here to interpret the effects...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pegram LM,Record MT Jr

更新日期：2006-09-26 00:00:00

abstract：:Deamidation of asparaginyl and glutaminyl residues causes time-dependent changes in charge and conformation of peptides and proteins. Quantitative and experimentally verified predictive calculations of the deamidation rates of 1,371 asparaginyl residues in a representative collection of 126 human proteins have been pe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson NE,Robinson AB

更新日期：2001-10-23 00:00:00

abstract：:Prenatal unilateral enucleation in mammals causes an extensive anatomical reorganization of visual pathways. The remaining eye innervates the entire extent of visual subcortical and cortical areas. Electrophysiological recordings have shown that the retino-geniculate connections are retinotopically organized and genic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bisti S,Trimarchi C

更新日期：1993-12-01 00:00:00

abstract：:Ral (Ras-like) GTPases are directly activated by oncogenic Ras GTPases. Mutant K-Ras (G12C) has enabled the development of covalent K-Ras inhibitors currently in clinical trials. However, Ral, and the overwhelming majority of mutant oncogenic K-Ras, are devoid of a druggable pocket and lack an accessible cysteine for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bum-Erdene K,Liu D,Gonzalez-Gutierrez G,Ghozayel MK,Xu D,Meroueh SO

更新日期：2020-03-31 00:00:00

abstract：:We have isolated a gene from Escherichia coli homologous to the gene encoding the Mr 83,000 Drosophila heat shock protein (hsp83). In E. coli the protein homologous to hsp83 is a heat shock protein called C62.5. The predicted amino acid sequence of C62.5 is 41% and 42% identical to the Drosophila and human hsp83 prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bardwell JC,Craig EA

更新日期：1987-08-01 00:00:00

abstract：:Anisotropic expansion of plant cells requires organized arrays of cortical microtubules. Mutations in microtubule-associated proteins and a particular mutation in alpha-tubulins were reported to cause abnormal microtubule arrays and result in helical growth in Arabidopsis thaliana. However, the way in which these muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishida T,Kaneko Y,Iwano M,Hashimoto T

更新日期：2007-05-15 00:00:00

abstract：:Osteogenin was recently purified and the amino acid sequences of tryptic peptides were determined. Osteogenin in conjunction with insoluble collagenous bone matrix induces cartilage and bone formation in vivo. To understand the mechanism of action of osteogenin, we examined its influence on periosteal cells, osteoblas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vukicevic S,Luyten FP,Reddi AH

更新日期：1989-11-01 00:00:00

abstract：:Population growth rate is a fundamental ecological and evolutionary characteristic of living organisms, but individuals must balance the metabolism devoted to biosynthesis and reproduction against the maintenance of existing structure and other functionality. Here we present a mathematical model that relates metabolic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kempes CP,Dutkiewicz S,Follows MJ

更新日期：2012-01-10 00:00:00

abstract：:Tubulin cofactor B (TCoB) plays an important role in microtubule dynamics by facilitating the dimerization of alpha- and beta-tubulin. Recent evidence suggests that p21-activated kinase 1 (Pak1), a major signaling nodule in eukaryotic cells, phosphorylates TCoB on Ser-65 and Ser-128 and plays an essential role in micr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rayala SK,Martin E,Sharina IG,Molli PR,Wang X,Jacobson R,Murad F,Kumar R

更新日期：2007-12-04 00:00:00

abstract：:During primate evolution, a major factor in lengthening life-span and decreasing age-specific cancer rates may have been improved protective mechanisms against oxygen radicals. We propose that one of these protective systems is plasma uric acid, the level of which increased markedly during primate evolution as a conse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ames BN,Cathcart R,Schwiers E,Hochstein P

更新日期：1981-11-01 00:00:00

abstract：:Agents that act as anion channel blockers (ACBs) and do not permeate cells appear to inhibit exocytosis in platelets, parathyroid cells, and neutrophils. Based in large part on these observations, anion influx through plasma membrane channels has been considered a factor controlling cellular secretion, but there have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vostal JG,Reid DM,Jones CE,Shulman NR

更新日期：1989-08-01 00:00:00

abstract：:In contrast to mechanisms mediating uptake of intracellular bacterial pathogens, bacterial egress and cell-to-cell transmission are poorly understood. Previously, we showed that the transmission of pathogenic mycobacteria between phagocytic cells also depends on nonlytic ejection through an F-actin based structure, ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerstenmaier L,Pilla R,Herrmann L,Herrmann H,Prado M,Villafano GJ,Kolonko M,Reimer R,Soldati T,King JS,Hagedorn M

更新日期：2015-02-17 00:00:00

abstract：:A detailed model for the structure and dynamics of the interior of the lipid bilayer in the liquid crystal phase is presented. The model includes two classes of motion: (i) the internal dynamics of the chains, determined from Brownian dynamics simulations with a continuous version of the Marcelja mean-field potential,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pastor RW,Venable RM,Karplus M

更新日期：1991-02-01 00:00:00