Abstract:
:A human metallothionein (MT) gene was inserted into a bovine papillomavirus (BPV) vector. The chimeric vector (pMTII-BPV) transforms rodent fibroblasts to a cadmium-resistant phenotype. The resistance is due to the high level of expression of human MT-II in those cells. The vector is maintained in the cells as a free replicating plasmid, present at about 10--15 copies per cell. Transcription of the episomal human MT-IIA gene is initiated from its authentic start sites and is regulated by the level of cadmium in the growth medium. The presence of the human MT-IIA gene allows the BPV replicon to function even though it is ligated to an intact copy of pBR322. Due to the presence of plasmid origins of replication and dominantly acting selective markers functional in both Escherichia coli and mammalian cells, pMTII-BPV can be used as a shuttle vector.
journal_name
Proc Natl Acad Sci U S Aauthors
Karin M,Cathala G,Nguyen-Huu MCdoi
10.1073/pnas.80.13.4040subject
Has Abstractpub_date
1983-07-01 00:00:00pages
4040-4issue
13eissn
0027-8424issn
1091-6490journal_volume
80pub_type
杂志文章abstract::After more than a decade of experiments generating and studying the physics of quantized vortices in atomic gas Bose-Einstein condensates, research is beginning to focus on the roles of vortices in quantum turbulence, as well as other measures of quantum turbulence in atomic condensates. Such research directions have ...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2014-03-25 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 临床试验,杂志文章
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更新日期:2014-07-29 00:00:00
abstract::A cyclophilin (CyP) purified to homogeneity from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 had a molecular mass of 20.5 kDa and a pI of 8.1. The protein catalyzed the isomerization of the prolyl peptide bond of N-succinyl-Ala-Ala-(cis,trans)-Pro-Phe p-nitroanilide with a kcat/Km value of 9.3 x...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1990-11-01 00:00:00