Abstract:
:Binding of the radioligand [125I]iodoclonazepam to three different areas of rat brain (cerebellum, hippocampus and striatum) has been characterised. In all three regions binding is rapid, saturable and of high affinity (cerebellum Bmax = 1.49 +/- 0.3 pmol/mg of protein, Kd = 0.39 +/- 0.06 nM; hippocampus Bmax = 1.5 +/- 0.14 pmol/mg of protein, Kd = 0.38 +/- 0.6 nM and striatum Bmax = 0.53 +/- 0.1 pmol/mg of protein, Kd = 0.34 +/- 0.03 nm, n = 3). In all regions only one population of sites was apparent. However, competition for [125I]iodoclonazepam sites by a series of benzodiazepine agonists and antagonists showed some regional differences. The BZ1 selective compounds zolpidem and CL218,872 showed a 4.3-fold and 5.2-fold selectivity for cerebellar binding sites respectively. We conclude that [125I]iodoclonazepam is a novel, high affinity ligand which recognises both BZ1 and BZ2 classes of receptor and should be a useful addition to the panel of benzodiazepine ligands currently available.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Gillard NP,Quirk K,Ragan CI,McKernan RMdoi
10.1016/0014-2999(91)90484-8keywords:
subject
Has Abstractpub_date
1991-04-03 00:00:00pages
407-9issue
3eissn
0014-2999issn
1879-0712pii
0014-2999(91)90484-8journal_volume
195pub_type
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journal_title:European journal of pharmacology
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