Abstract:
OBJECTIVE:To evaluate the phenotype, proliferative, and differentiation capacities in vitro of stromal cells derived from peritoneal, ovarian, and deeply infiltrating endometriosis. DESIGN:Experimental study using phase contrast microscopy, immunocytochemistry, and functional bioassays. SETTING:University-based laboratory. PATIENT(S):Women with and without endometriosis undergoing surgery for benign indications. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):The stability in vitro of stromal cells derived from peritoneal (n = 18), ovarian (n = 29), and deeply infiltrating (n = 14) endometriotic lesions, as well as endometrium from women with (n = 5) and without endometriosis (n = 5) was evaluated by detection of endometrial markers. The proliferative and differentiation capacity of the cells was assessed by the use of cell doubling estimation and in vitro decidualization assays. RESULT(S):The expression of the progesterone receptor and CD10 in stromal cells derived from the three types of endometriotic lesions is retained in culture up to passage 10. The doubling time of stromal cells from deeply infiltrating lesions is lower than that of endometrial stromal cells. Levels of prolactin and insulin-like growth factor binding protein-1 (IGFBP-1) are reduced in supernatants from stromal cells derived from the three types of lesions and from the endometrium of women with endometriosis. CONCLUSION(S):The peritoneal, ovarian, and deeply infiltrating endometriotic stromal cell lines we describe retain in vivo tissue markers. Loss of differentiation capacity of the endometriotic cell lines and endometrial cells from women with endometriosis may influence the capacity for proliferation and survival of these cells in the ectopic environment.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Klemmt PA,Carver JG,Kennedy SH,Koninckx PR,Mardon HJdoi
10.1016/j.fertnstert.2005.08.046keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
564-72issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(05)03975-0journal_volume
85pub_type
杂志文章abstract:OBJECTIVE:To test for an association between DNA fragmentation and head shape at high magnification in fresh motile spermatozoa. DESIGN:Observational study. SETTING:Academic tertiary care center. PATIENT(S):A total of 60 men in our assisted reproductive program. INTERVENTION(S):Quantifying sperm head shape using el...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.01.100
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVE:To determine whether endometriosis is associated with mitochondrial dysfunction in cumulus (granulosa [GC]) cells of subjects undergoing IVF-intracytoplasmic sperm injection (ICSI). DESIGN:Prospective cohort study. SETTING:An IVF clinic in a tertiary academic care center. PATIENT(S):Eleven women with endom...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.11.002
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of a pill with drospirenone (3 mg) plus ethinyl E(2) (20 μg) (DRP/20EE) alone or associated with metformin or cyproterone acetate (CPA) on some metabolic cardiovascular risk factors in women with polycystic ovary syndrome (PCOS). DESIGN:Randomized, open-label clinical trial. SETTING:...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2009.10.016
更新日期:2010-10-01 00:00:00
abstract::Testosterone and 19-nortestosterone derivatives were evaluated in a developmental scheme designed to identify competitive progesterone antagonists having abortifacient activity. Compounds that displayed significant binding to the rabbit uterine progesterone receptor were followed in biologic tests for progestational, ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44003-3
更新日期:1979-05-01 00:00:00
abstract:OBJECTIVE:To report a case of severe ovarian hyperstimulation syndrome (OHSS) after inadvertent GnRH long protocol/hMG stimulation in a pre-existing early pregnancy. DESIGN:Case report. SETTING:Private infertility clinic. PATIENT(S):A 28-year-old woman who conceived spontaneously following IVF and cryo-embryo transf...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.05.069
更新日期:2005-12-01 00:00:00
abstract:OBJECTIVE:To assess the association between transsexualism and allele and genotype frequencies of the common cytochrome P450 (CYP) 17 -34 T>C single nucleotide polymorphism (SNP). DESIGN:Case-control study. SETTING:Academic research institution. PATIENT(S):102 male-to-female (MtF) and 49 female-to-male (FtM) transse...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.05.056
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of lipopolysaccharide (LPS) on the expression of tumor necrosis factor alpha (TNFalpha) and interleukin-8 (IL-8) protein in endometriotic stromal cells (ESC) and their effect on the proliferation of ESC. DESIGN:Prospective study. SETTING:Department of Obstetrics and Gynecology, Tottor...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.04.038
更新日期:2004-10-01 00:00:00
abstract:OBJECTIVE:To investigate whether Kremer's sperm-mucus penetration test may predict sperm fertilizing ability in IVF. DESIGN:Kremer's test was prospectively performed on semen samples used for 66 consecutive IVF trials and compared with the fertilization rates and fertilization failure rates observed. RESULTS:Fertiliz...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55981-0
更新日期:1993-06-01 00:00:00
abstract::The author reports on 206 cases of endometriosis examined between 1970-1977. 172 patients had used OC (oral contraception) for a varying period of time prior to diagnosis of endometriosis; of these, 122 had taken only combined OCs containing 50 mg. or more of estrogen. These data neither support nor contradict the c...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)43887-2
更新日期:1979-03-01 00:00:00
abstract::Human GCs obtained from patients undergoing an IVF procedure and treated with CC, hMG, and hCG, lack cAMP responsiveness to hCG stimulation in vitro. After 48 hours in culture, recovery of cAMP production was significantly higher in hCG-stimulated cells grown on dishes coated with ECM than in cells grown on uncoated d...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49596-8
更新日期:1986-09-01 00:00:00
abstract:OBJECTIVE:To compare androgen responses during ACTH infusion among women with polycystic ovary syndrome (PCOS) and healthy women. DESIGN:Cross-sectional study. SETTING:Academic medical center. PATIENT(S):Women with PCOS (n = 13) and healthy controls (n = 15). INTERVENTION(S):Blood samples were obtained frequently d...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.06.039
更新日期:2016-10-01 00:00:00
abstract:OBJECTIVE:To assess the relevant factors affecting patients' decision when choosing a clinic for private, self-funded IVF treatment. DESIGN:Prospective anonymous closed questionnaires. SETTING:Single tertiary-care private IVF center. PATIENT(S):New patients attending primary consultation in a period of 7 months from...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)01751-4
更新日期:2001-05-01 00:00:00
abstract:OBJECTIVE:To review the mechanisms of T replacement therapy's inhibition of spermatogenesis and current therapeutic approaches in reproductive aged men. DESIGN:Review of published literature. SETTING:PubMed search from 1990-2012. PATIENT(S):PubMed search from 1990-2012. INTERVENTION(S):A literature review was perfo...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2012.10.052
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVE:To study the effects of hormone therapy on brain activation patterns during verbal memory in postmenopausal women. DESIGN:A randomized, double-blind placebo-controlled cross-over study was performed. SETTING:A tertiary care university medical center. PATIENT(S):Ten healthy postmenopausal women (age range 5...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2008.04.040
更新日期:2009-07-01 00:00:00
abstract:OBJECTIVE:To evaluate novel clinical markers of endometriosis including the neurotrophins brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and neurotrophin 4/5 (NT4/5) and compare them to others previously reported in the literature including cancer antigen 125 (CA-125) and C-reactive protein (CRP)....
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.09.003
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:To present a case of a unilateral endometrioma with extremely elevated serum CA-125 levels. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 25-year-old woman with left adnexal mass and extremely elevated serum CA-125 level, 7,900 U/mL, underwent laparoscopy. There were unilateral endometrioma...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.12.076
更新日期:2007-10-01 00:00:00
abstract:OBJECTIVE:To manipulate the luteal endometrial progression by the use of antiprogestins. DESIGN:Prospective controlled clinical trial. SETTING:The IVF program of the University of Southern California School of Medicine, Los Angeles, California. PATIENT(S):Thirteen oocyte donors and 20 oocyte recipients. INTERVENTIO...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/S0015-0282(97)81918-8
更新日期:1997-02-01 00:00:00
abstract::The complete lack of information about mast cells as a source of histamine and potential target cells for histamine in human testes prompted us to investigate these issues in testes of fertile and infertile patients using a combination of laser microdissection, reverse transcription-polymerase chain reaction (RT-PCR),...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.12.002
更新日期:2005-04-01 00:00:00
abstract:OBJECTIVE:To report the case of a patient submitted to laparoscopic bowel resection in whom histology revealed endometriosis and schistosomiasis. DESIGN:Case report. SETTING:Multidisciplinary group practice and teaching hospital. PATIENT(S):A 31-year-old patient with pelvic pain. INTERVENTION(S):Laparoscopic bowel ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.08.068
更新日期:2006-04-01 00:00:00
abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.08.053
更新日期:2013-10-07 00:00:00
abstract::This study investigated the prevalence of Tyr204Phe and Val34Leu polymorphisms in two Brazilian ethnic groups (171 Caucasians and 27 Blacks, and 117 men and 81 women) and in patients with recurrent miscarriages (RM) (86 women: 53 Caucasians and 33 Blacks). Study groups were matched to control groups by race and age. T...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.04.052
更新日期:2004-11-01 00:00:00
abstract:OBJECTIVE:To describe a patient with uterine rupture in the subsequent pregnancy after hysteroscopic resection of a uterine septum. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 30-year-old nulliparous woman, with a history of a first trimester spontaneous abortion, hysteroscopic uterine septum resec...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.08.075
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:To measure serum anti-Müllerian hormone (AMH) during different phases of the menstrual cycle and to correlate the measurements with ovarian response and clinical-pregnancy rates in intracytoplasmic sperm injection cycles. DESIGN:Prospective cohort study. SETTING:University IVF unit. PATIENT(S):Thirty-three...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.05.040
更新日期:2008-06-01 00:00:00
abstract::Acute hyperandrogenism decreases serum P levels and induces early apoptosis of antral follicles by a mechanism mediated by the peroxisome proliferator-activated receptor gamma system and independent of the steroidogenic acute regulator protein. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.07.1083
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To reassess the predictive value of detecting sperm cells in men with AZFb or AZFb-c deletions. DESIGN:Retrospective analysis of previously reported men with AZFb or AZFb-c deletions and the addition of six new cases. SETTING:Fertility institution. PATIENT(S):Men with both sequence tagged site marker ident...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2011.01.162
更新日期:2011-05-01 00:00:00
abstract:OBJECTIVE:To review selected environmental, occupational, and other important risk factors for the following adverse pregnancy outcomes: low birth weight (LBW), intrauterine growth retardation (IUGR), and preterm delivery (PTD). DESIGN:The evidence is explored in greater detail for environmental tobacco smoke, drinkin...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2007.12.041
更新日期:2008-02-01 00:00:00
abstract::Transvaginal follicular aspiration (TVA) with ultrasonically guided needles allows the transfer of in vitro generated embryos to the fallopian tubes (TET), performing only one surgical procedure in the process. Up to now, this approach has been used to treat 16 couples with infertility due to severe male factor. Folli...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60136-x
更新日期:1988-09-01 00:00:00
abstract:OBJECTIVE:To evaluate binding characteristics of a specific zona pellucida (ZP) protein 3 (ZP3) antiserum to human oocytes in order to determine its usefulness as a clinical marker for human ZP integrity and function and its correlation with IVF outcome. DESIGN:Prospectively designed, blinded, internally controlled st...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)58041-8
更新日期:1996-01-01 00:00:00
abstract::Cervical smears were taken before, and at intervals after, artificial insemination and scored for the ratio of leukocytes to squamous cells. Most women showed a significant rise in this ratio after insemination with whole fresh or frozen/thawed semen, but this was not seen when fresh or frozen sperm-free seminal plasm...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48442-6
更新日期:1985-03-01 00:00:00
abstract:OBJECTIVE:To examine the emotional, sexual, physical, and quality-of-life (QOL) impact on infertile women awaiting oocyte donation. DESIGN:Cross-sectional cohort study. SETTING:Cancer center and reproductive medicine center. PATIENT(S):Fifty infertile women awaiting oocyte donation, enrolled from October 2006 to Feb...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.10.004
更新日期:2011-02-01 00:00:00