Carcinoembryonic antigen regulation in human colorectal tumor cells by a site-selective cyclic AMP analogue: a comparison with interferon-gamma.


:Treatment of human colorectal tumor cells (LS174T, HT-29, and WiDr) with analogues of cyclic AMP (cAMP) (dibutyryl-cAMP and 8-Cl-cAMP) selectively enhances the expression of carcinoembryonic antigen (CEA). Dose and temporal kinetics results revealed that 8-Cl-cAMP was approximately 100-fold more potent than dibutyryl-cAMP for increasing CEA expression. Results demonstrated that 8-Cl-cAMP treatment of LS174T quantitatively increased CEA levels in cell extracts 2-fold, increased anti-CEA monoclonal antibody (MAb) binding to the tumor cell surface, and induced the appearance of CEA-related mRNA transcripts. The findings suggest that 8-Cl-cAMP is capable of regulating CEA expression at transcriptional and/or post-transcriptional levels. Other human tumor cells, as well as normal cell types which do not constitutively express CEA, remained CEA-negative following 8-Cl-cAMP treatment. Moreover, the level of expression of other human tumor antigens as well as antigens of the major histocompatibility complex were not changed by 8-Cl-cAMP treatment, suggesting some selectivity for CEA regulation by this cAMP analogue. In vivo administration of 8-Cl-cAMP to athymic mice bearing LS174T tumor xenografts increased the amount of anti-CEA MAb bound to tumor extracts as well as the tumor localization of a radionuclide-conjugated anti-CEA MAb. The results indicate that 8-Cl-cAMP can selectively upregulate CEA expression on human colorectal tumor cells in vitro and in vivo. Interestingly, IFN-gamma treatment of the LS174T cells fails to enhance or induce expression of CEA or any of the histocompatibility leukocyte antigens. Thus, 8-Cl-cAMP treatment regulates CEA expression through another cellular pathway which may involve cAMP-dependent protein kinase.


Int J Cancer


Guadagni F,Tortora G,Roselli M,Clair T,Cho-Chung YS,Schlom J,Greiner JW





Has Abstract


1991-05-30 00:00:00












  • Effect of aspirin on cell proliferation and differentiation of colon adenocarcinoma Caco-2 cells.

    abstract::Several lines of evidence suggest that long-term treatment with non-steroidal anti-inflammatory drugs may reduce the risk of colon cancer and the size and number of colonic polyps in patients with familial adenomatous polyposis. Aspirin has also been shown to inhibit cell proliferation in human tumor cell lines and to...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ricchi P,Pignata S,Di Popolo A,Memoli A,Apicella A,Zarrilli R,Acquaviva AM

    更新日期:1997-12-10 00:00:00

  • Endocrine sensitivity of the receptor-positive T61 human breast carcinoma serially grown in nude mice.

    abstract::A study was made on the effect of ovariectomy, 17 beta-oestradiol, and tamoxifen on the oestrogen and progesterone receptor-positive T61 human breast carcinoma grown in nude mice. The effect of the treatment was evaluated by the specific growth delay calculated on the basis of Gompertz growth curves, and by the change...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Brünner N,Spang-Thomsen M,Skovgaard Poulsen H,Engelholm SA,Nielsen A,Vindeløv L

    更新日期:1985-01-15 00:00:00

  • Assessment of highly angiogenic and disseminated in the peripheral blood disease in breast cancer patients predicts for resistance to adjuvant chemotherapy and early relapse.

    abstract::The assessment of tumor molecular features in combination with the detection of occult malignant cells may provide important clinical information, beyond the standard staging of breast cancer. Using a nested RT-PCR technique, we assessed prospectively the presence of cytokeratin-19 (CK19) mRNA positive cells in the bl...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Giatromanolaki A,Koukourakis MI,Kakolyris S,Mavroudis D,Kouroussis C,Mavroudi C,Perraki M,Sivridis E,Georgoulias V

    更新日期:2004-02-10 00:00:00

  • TOB suppresses breast cancer tumorigenesis.

    abstract::Transducer of ErbB-2 (TOB) is a member of the TOB/Btg gene family. A role for TOB in the suppression of human tumorigenesis has been proposed, based on the observations that TOB-knockout mice spontaneously form tumors and TOB expression is lost in human lung and thyroid cancers. However, the role of TOB in human breas...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: O'Malley S,Su H,Zhang T,Ng C,Ge H,Tang CK

    更新日期:2009-10-15 00:00:00

  • Imatinib mesylate effectively combines with chaperone-rich cell lysate-loaded dendritic cells to treat bcr-abl+ murine leukemia.

    abstract::Imatinib mesylate has become an effective agent for the treatment of chronic myeloid leukemia (CML). However, the development of drug resistance has led to examination of combination therapies. In this study, we investigated the effects of combining imatinib with immunotherapy against a murine bcr-abl(+) leukemia, 12B...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zeng Y,Graner MW,Feng H,Li G,Katsanis E

    更新日期:2004-06-10 00:00:00

  • Promoter -202 A/C polymorphism of insulin-like growth factor binding protein-3 gene and non-small cell lung cancer risk.

    abstract::Insulin-like growth factor binding protein-3 (IGFBP-3) inhibits the mitogenic and antiapoptotic activity of insulin-like growth factor (IGF) by blocking the binding of IGF to its receptor. However, under certain circumstances, IGFBP-3 can enhance the activity of IGF by protecting IGF from degradation. More than half o...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Moon JW,Chang YS,Ahn CW,Yoo KN,Shin JH,Kong JH,Kim YS,Chang J,Kim SK,Kim HJ,Kim SK

    更新日期:2006-01-15 00:00:00

  • Metallothionein gene expression and resistance to cisplatin in human ovarian cancer.

    abstract::Intracellular thiols have been proposed as mediators of resistance to alkylating agents and cisplatin. As metallothionein is the predominant protein thiol, we examined its relationship to cisplatin resistance in human ovarian cancer cell lines. A human ovarian carcinoma cell line, A2780, derived from an untreated pati...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Schilder RJ,Hall L,Monks A,Handel LM,Fornace AJ Jr,Ozols RF,Fojo AT,Hamilton TC

    更新日期:1990-03-15 00:00:00

  • Cancer registration and healthcare access in West Bank, Palestine: a GIS analysis of childhood cancer, 1998-2007.

    abstract::In low and middle income countries (LMIC), high-quality disease registration is difficult to achieve in the setting of inadequate healthcare infrastructure and political or economical instability. In this article, we explore the potential of geographic information systems (GIS) to add value to the understanding of chi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bailony MR,Hararah MK,Salhab AR,Ghannam I,Abdeen Z,Ghannam J

    更新日期:2011-09-01 00:00:00

  • Factors associated with incident and fatal pancreatic cancer in a cohort of middle-aged women.

    abstract::Risk factors for pancreatic cancer, other than smoking and diabetes, are not well-established, especially for women. In a cohort of 1.3 million middle-aged women, followed for 9.2 million person-years for cancer incidence and 11.5 million person-years for mortality, there were 1,338 incident pancreatic cancer cases an...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Stevens RJ,Roddam AW,Spencer EA,Pirie KL,Reeves GK,Green J,Beral V,Million Women Study Collaborators.

    更新日期:2009-05-15 00:00:00

  • Cancer cell-targeted drug delivery utilizing oligopeptide transport activity.

    abstract::To study the drug delivery to tumor by utilization of an oligopeptide transport activity, we examined the accumulation of dipeptides and the peptide-mimetic anti-cancer drug, bestatin, a substrate of oligopeptide transporter PepT1. Firstly, we established HeLa cells stably expressing human peptide transporter (hPepT1)...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Nakanishi T,Tamai I,Takaki A,Tsuji A

    更新日期:2000-10-15 00:00:00

  • Heterotransplantation of small-cell carcinoma of the lung into nude mice: comparison of intracranial and subcutaneous routes.

    abstract::We compared and contrasted intracranial (i.c.) and subcutaneous (s.c.) heterotransplantation of small-cell carcinoma of the lung (SCCL) into athymic nude mice. Fresh human SCCL tumor specimens, tumor colonies grown in soft agarose and continuous cell lines were used. Tumors induced by the three types of specimens were...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gazdar AF,Carney DN,Sims HL,Simmons A

    更新日期:1981-12-01 00:00:00

  • Metastatic potential of murine fibrosarcoma cells is influenced by cell surface laminin.

    abstract::In order to examine the role of cell surface laminin in tumor metastasis we have utilized four well-characterized murine fibrosarcoma cell lines. Two of these lines were highly metastatic when injected into syngeneic mice while the remaining two lines were significantly less metastatic. Using indirect immunofluorescen...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Malinoff HL,McCoy JP Jr,Varani J,Wicha MS

    更新日期:1984-05-15 00:00:00

  • Anxiety and distress following receipt of results from routine HPV primary testing in cervical screening: The psychological impact of primary screening (PIPS) study.

    abstract::We used a cross-sectional survey to examine short-term anxiety and distress in women receiving different results following routine human papillomavirus (HPV) primary testing at cervical screening. Participants were women aged 24-65 (n = 1,127) who had attended screening at one of five sites piloting HPV primary screen...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: McBride E,Marlow LAV,Forster AS,Ridout D,Kitchener H,Patnick J,Waller J

    更新日期:2020-04-15 00:00:00

  • In vitro and in vivo modulation of multi-drug resistance with amiodarone.

    abstract::The modulating effect on drug resistance of amiodarone (AM) and its metabolite desethylamiodarone (DEA) was studied in a P-glycoprotein-positive human colon carcinoma cell line COLO 320, and a human small-cell lung carcinoma cell line GLC4 and its adriamycin (Adr)-resistant subline GLC4-Adr (both P-glycoprotein-negati...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: van der Graaf WT,de Vries EG,Uges DR,Nanninga AG,Meijer C,Vellenga E,Mulder PO,Mulder NH

    更新日期:1991-06-19 00:00:00

  • Melanoma progression-associated glycoprotein MUC18/MCAM mediates homotypic cell adhesion through interaction with a heterophilic ligand.

    abstract::MUC18/MCAM is a cell-surface glycoprotein that is strongly expressed on advanced human melanomas. Transfection of 3 MCAM-negative melanoma cell lines with MCAM cDNA led to cell-surface expression and to a MCAM-dependent homotypic adhesion. This adhesion was independent of divalent cations and was inhibited at 4 degree...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Johnson JP,Bar-Eli M,Jansen B,Markhof E

    更新日期:1997-11-27 00:00:00

  • Vitamin D binding protein and risk of renal cell carcinoma in the prostate, lung, colorectal and ovarian cancer screening trial.

    abstract::Our group has conducted two previous studies on the association between vitamin D binding protein (DBP) and renal cell carcinoma (RCC), the most common form of kidney cancer, finding strong inverse associations. We undertook the current analysis to replicate our findings in a different study population that included w...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kratzer TB,Weinstein SJ,Albanes D,Mondul AM

    更新日期:2020-08-01 00:00:00

  • Inhibition by lymphoblastoid interferon of growth of cells derived from the human breast.

    abstract::Human lymphoblastoid interferon inhibited the growth in vitro of fibroblasts and epithelial cells from normal, hyperplastic and neoplastic human breast tissue. At an interferon concentration of 10(3) inter-national units (IU) per ml, the inhibitory effects on monolayer growth were completely reversible but the growth ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Balkwill F,Watling D,Taylor-Papadimitriou J

    更新日期:1978-09-15 00:00:00

  • In vivo induction of endogenous retroviruses in BALB/c mouse hepatocytes by successive treatments with carbon tetrachloride and bromodeoxyuridine.

    abstract::The effects of bromodeoxyuridine (BrdUrd) incorporation into hepatocytes of carbon tetrachloride (CCl4)-induced regenerating liver of BALB/c mice on the induction of endogenous retroviruses was examined. From the nucliec acid hybridization studies, the maximum levels of hybridization were obtained for both N- and X-tr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ayukawa HM,Saltzburg D,Mukherjee BB

    更新日期:1979-10-15 00:00:00

  • International patterns and trends in testis cancer incidence.

    abstract::Although the incidence of testis cancer has risen markedly in many Western populations over the past half-century, it is not clear whether rates in other populations also have increased. To clarify this issue, we examined testis cancer incidence rates over the 25-year time period of 1973-1997 for selected populations ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Purdue MP,Devesa SS,Sigurdson AJ,McGlynn KA

    更新日期:2005-07-10 00:00:00

  • Cross-signaling among phosphinositide-3 kinase, mitogen-activated protein kinase and sonic hedgehog pathways exists in esophageal cancer.

    abstract::The hedgehog (Hh) signaling pathway is essential for the development of tissues and organs. Hyperactive Hh signaling has been implicated in many gastric cancers, including esophageal cancer. However, the interaction between the Hh pathway and other potential signaling pathways in primary esophageal tumorigenesis has n...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wei L,Xu Z

    更新日期:2011-07-15 00:00:00

  • Dysbiosis of the endometrial microbiota and its association with inflammatory cytokines in endometrial cancer.

    abstract::The underlying molecular mechanisms involved in the pathogenesis of endometrial cancer (EC) are still not well understood. Our goal was to investigate the composition of the endometrial microbiota and the association with inflammatory cytokines in EC. Endometrial microbiota profiles of women with EC (n = 25) and benig...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lu W,He F,Lin Z,Liu S,Tang L,Huang Y,Hu Z

    更新日期:2020-12-07 00:00:00

  • HPP1-mediated tumor suppression requires activation of STAT1 pathways.

    abstract::HPP1 is a recently discovered gene that is epigenetically silenced in a number of tumor types, suggesting a potential role as a tumor suppressor. However, whether HPP1 has tumor suppressor activity is not clearly known. We have sought to investigate the effects of HPP1 on tumor growth and survival and to identify sign...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Elahi A,Zhang L,Yeatman TJ,Gery S,Sebti S,Shibata D

    更新日期:2008-04-01 00:00:00

  • Apigenin inhibits endothelial-cell proliferation in G(2)/M phase whereas it stimulates smooth-muscle cells by inhibiting P21 and P27 expression.

    abstract::Apigenin is a plant flavonoid that is thought to play a role in the prevention of carcinogenesis. However, its mechanism of action has not yet been elucidated. Because of the importance of angiogenesis in tumor growth, we investigated the effect of apigenin on endothelial and smooth-muscle cells in an in vitro model. ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Trochon V,Blot E,Cymbalista F,Engelmann C,Tang RP,Thomaïdis A,Vasse M,Soria J,Lu H,Soria C

    更新日期:2000-03-01 00:00:00

  • Breast cancer and microbial cancer incidence in female populations around the world: a surprising hyperbolic association.

    abstract::Current literature on cancer epidemiology typically discusses etiology of cancer by cancer type. Risks of different cancer types are, however, correlated at population level and may provide etiological clues. We showed previously an unexpected very high positive correlation between breast cancer (BC) and young-adult H...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Savu A,Potter J,Li S,Yasui Y

    更新日期:2008-09-01 00:00:00

  • Hepatitis C virus 1b is the dominant genotype in HCV-related carcinogenesis: a case-control study.

    abstract::In an ongoing case-control study in Athens on the etiology of hepatocellular carcinoma (HCC), an analysis was made in order to assess whether HCV genotype 1b is associated with hepatocellular carcinoma (HCC). The HCV genotype was determined in 17 HCC patients, 87 patients with chronic hepatitis C (CHC) without cirrhos...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hatzakis A,Katsoulidou A,Kaklamani E,Touloumi G,Koumantaki Y,Tassopoulos NC,Karvountzis G,Gioustozi A,Hadziyannis S,Trichopoulos D

    更新日期:1996-09-27 00:00:00

  • Syngeneic monoclonal antibodies directed against Rauscher virus-induced myeloid leukemic cells: isolation and characterization.

    abstract::Hybridomas producing syngeneic monoclonal antibodies (MAbs) were prepared by fusion of spleen cells of BALB/c mice, which were immunized with sublethal doses of RMB-I cells. This cell line originates from a Rauscher virus (R-MuLV)-induced myeloid leukemia and forms tumors when re-inoculated into mice. MAbs were charac...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Berends D,Rhijnsburger EH,van Gaalen JL,van Houwelingen G,Zondervan PE,de Both NJ

    更新日期:1988-07-15 00:00:00

  • Altered expression of CD44 isoforms in squamous-cell carcinomas and cell lines derived from them.

    abstract::CD44 is a transmembrane glycoprotein that binds hyaluronan, extracellular matrix proteins and growth factors. Multiple isoforms of CD44 are generated by alternative splicing of 10 separate exons (V1-V10). Expression of the variable exons has been correlated with tumour progression and metastasis in a range of cell typ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hudson DL,Speight PM,Watt FM

    更新日期:1996-05-16 00:00:00

  • Modification of the inverse association between dietary vitamin D intake and colorectal cancer risk by a FokI variant supports a chemoprotective action of Vitamin D intake mediated through VDR binding.

    abstract::Vitamin D has anticarcinogenic properties and might influence colorectal cancer (CRC) risk, but the epidemiological evidence is inconsistent. Many mechanisms of action for vitamin D have been proposed, with some of them initiating via its binding to the vitamin D receptor (VDR). Using a large Scottish case-control stu...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Theodoratou E,Farrington SM,Tenesa A,McNeill G,Cetnarskyj R,Barnetson RA,Porteous ME,Dunlop MG,Campbell H

    更新日期:2008-11-01 00:00:00

  • Efficacy of intra-hepatectomy 5-FU on recurrence and metastasis of human hepatocellular carcinoma in nude mice.

    abstract::A novel intra-operative chemotherapy nude mouse model for human hepatocellular carcinoma (HCC) has been developed. Intra-peritoneal (i.p.) administration of 5-fluorouracil (5-FU) was begun 2 hr before hepatic resection of HCC and then continued post-operatively for 4 consecutive days. This regime, termed intra-hepatec...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rashidi B,An Z,Sun FX,Li X,Tang ZY,Moossa AR,Hoffman RM

    更新日期:2001-01-15 00:00:00

  • Cytoskeleton and paclitaxel sensitivity in breast cancer: the role of beta-tubulins.

    abstract::The antineoplastic effect of paclitaxel is mainly related to its ability to bind the beta subunit of tubulin, thus preventing tubulin chain depolarization and inducing apoptosis. The relevance of the Class I beta-tubulin characteristics have also been confirmed in the clinical setting where mutations of paclitaxel-bin...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tommasi S,Mangia A,Lacalamita R,Bellizzi A,Fedele V,Chiriatti A,Thomssen C,Kendzierski N,Latorre A,Lorusso V,Schittulli F,Zito F,Kavallaris M,Paradiso A

    更新日期:2007-05-15 00:00:00