A cell-free biochemical complementation assay reveals complex and redundant cytosolic requirements for LRP endocytosis.

abstract：:The DNA topoisomerase I inhibitor camptothecin (CAM) is selectively cytotoxic to S-phase cells of HL-60, and some other myelogenous leukemic lines. The early effects of cell exposure to 0.05-0.2 micrograms/ml CAM are seen after 2 h; at that time a progressive degradation of DNA in the chromatin of S-phase cells is ini...

journal_title：Experimental cell research

authors： Del Bino G,Lassota P,Darzynkiewicz Z

更新日期：1991-03-01 00:00:00

abstract：:Hypoxic conditions are common in solid tumors and have a significant effect on tumor progression, therapeutic and prognosis. Long noncoding RNAs (lncRNAs) are longer than 200 nucleotides and cannot be translated into proteins, which play important roles in some diseases including cancer. Although previous analysis hav...

journal_title：Experimental cell research

authors： Shen Y,Liu Y,Sun T,Yang W

更新日期：2017-09-15 00:00:00

abstract：:Trophoblast cells were grown on filters that allow access to apical and basal surfaces of cells. Using this experimental system, IgG transport was shown to be specific and to occur primarily in the apical to basal direction. This transport was time- and temperature-dependent, with approximately 10% of added IgG appear...

journal_title：Experimental cell research

authors： Sooranna SR,Contractor SF

更新日期：1991-01-01 00:00:00

abstract：:The linker histone subtype H1.1 belongs to the group of main-type histones and is synthesized in somatic tissues as well as in germ cells during the S phase of the cell cycle. In adult mice the histone gene H1.1 is expressed mainly in thymus, spleen, and testis. The single-copy gene coding for the H1.1 protein was eli...

journal_title：Experimental cell research

authors： Rabini S,Franke K,Saftig P,Bode C,Doenecke D,Drabent B

更新日期：2000-02-25 00:00:00

abstract：:SIRT5 has a wide range of functions in different cellular processes such as glycolysis, TCA cycle and antioxidant defense, which mediates lysine desuccinylation, deglutarylation and demalonylation. Recent evidences have implicated that SIRT5 is a potential suppressor of gastric cancer (GC). However, the underlying mec...

journal_title：Experimental cell research

authors： Lu X,Yang P,Zhao X,Jiang M,Hu S,Ouyang Y,Zeng L,Wu J

更新日期：2019-09-15 00:00:00

abstract：:We have tested whether life-long caloric restriction (CR) slows or delays the age-related loss of cellular replicative potential that occurs during normal aging in ad libitum (AL) fed mice. Both mean and maximum life spans of the restricted animals (60% of AL intake) were significantly extended 30-40% by CR treatment....

journal_title：Experimental cell research

authors： Pendergrass WR,Li Y,Jiang D,Fei RG,Wolf NS

更新日期：1995-04-01 00:00:00

abstract：:Protein kinase B (Akt1) holds a central role for cellular growth, development, and survival, but the cellular pathways of Akt1 that prevent inflammatory demise in the vascular system remain undefined. Employing a constitutively active form of Akt1 (myristoylated Akt1) in endothelial cells (ECs), we demonstrate that Ak...

journal_title：Experimental cell research

authors： Chong ZZ,Kang JQ,Maiese K

更新日期：2004-06-10 00:00:00

abstract：:Intramuscular adipose tissue and fibrous tissue are observed in some skeletal muscle pathologies such as Duchenne muscular dystrophy and sarcopenia, and affect muscle strength and myogenesis. They originate from common fibrogenic/adipogenic cells in the skeletal muscle. Thus, elucidating the regulatory mechanisms unde...

journal_title：Experimental cell research

authors： Takeuchi S,Nakano S,Nakamura K,Ozoe A,Chien P,Yoshihara H,Hakuno F,Matsuwaki T,Saeki Y,Takahashi S,Yamanouchi K,Nishihara M

更新日期：2016-10-01 00:00:00

abstract：:The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, ra...

journal_title：Experimental cell research

authors： Wang J,Tolan DR,Pagliaro L

更新日期：1997-12-15 00:00:00

abstract：:Overexpression of P-glycoprotein may cause increased efflux of a variety of anticancer drugs (ACD) leading to multidrug resistance (MDR) of tumor cells. Two sublines of murine monocytic leukemia P388 cells were used, one parental (Par-P388) and one multidrug resistant (MDR-P388). In cell growth inhibition assays in vi...

journal_title：Experimental cell research

authors： Boesch D,Muller K,Pourtier-Manzanedo A,Loor F

更新日期：1991-09-01 00:00:00

abstract：:Phosphorylation of the nucleosome core histone H3 (H3) on Ser-10 is thought to be a prerequisite for chromatin condensation at mitosis. Although during interphase, cell differentiation, or mitogenic activation of quiescent cells, changes in chromatin structure that involve local chromatin condensation/decondensation a...

journal_title：Experimental cell research

authors： Juan G,Traganos F,Darzynkiewicz Z

更新日期：1999-01-10 00:00:00

abstract：:Transforming growth factor beta (TGFbeta) can modulate the activity of various MAP kinases. However, how this pathway may mediate TGFbeta-induced malignant phenotypes remains elusive. We investigated the role of autocrine TGFbeta signaling through MAP kinases in the regulation of cell survival in breast carcinoma MCF-...

journal_title：Experimental cell research

authors： Lei X,Yang J,Nichols RW,Sun LZ

更新日期：2007-05-01 00:00:00

abstract：:Transcription factors belonging to the basic helix-loop-helix Per-Arnt-Sim (bHLH/PAS) family control a wide variety of biological processes in mammalian and/or Drosophila. We have previously isolated bHLH/PAS Xenopus amphibian homologs of Single-minded (xSim) and aryl receptor nuclear translocator (xArnt) and characte...

journal_title：Experimental cell research

authors： Coumailleau P,Bollérot K,Lecomte C,Angelier N

更新日期：2003-07-15 00:00:00

abstract：:Hydrogen peroxide is more toxic to mammalian cells at 37 degrees C than 0 degree C at all concentrations studied. Histone-free nuclei (nucleoids) extracted from treated cells have a reduced ability to maintain positive DNA supercoiling, with the maximum effect at the higher temperature. Prior exposure of cells to sodi...

journal_title：Experimental cell research

authors： Vaughan AT,Gordon DJ

更新日期：1992-10-01 00:00:00

abstract：:In human peripheral blood mononuclear cells, ciliary neurotrophic factor (CNTF) weakly suppressed endotoxin-induced interleukin (IL)-1 and prostaglandin E2(PGE2). Suppression of PGE2 and IL-8 synthesis was significantly greater (up to 42.6%, P < 0.05) by adding a 10-fold molar excess of soluble CNTF receptor (sCNTFR a...

journal_title：Experimental cell research

authors： Shapiro L,Panayotatos N,Meydani SN,Wu D,Dinarello CA

更新日期：1994-11-01 00:00:00

abstract：:Local regulation of the cortical cytoskeleton controls cell surface dynamics. GAP-43 and MARCKS are two abundant cytosolic protein kinase C substrates that are anchored to the cell membrane via acyl groups and interact with the cortical cytoskeleton. Each of them has been implicated in several forms of motility involv...

journal_title：Experimental cell research

authors： Wiederkehr A,Staple J,Caroni P

更新日期：1997-10-10 00:00:00

abstract：:Nano-sized Gram-negative bacterial outer membrane vesicles possess unique structural and immunostimulatory effects that could be exploited to regress tumors by alerting the host immune system and reversing the immunosuppressive tumor microenvironment. The current study was conducted to investigate the antitumor activi...

journal_title：Experimental cell research

authors： Aly RG,El-Enbaawy MI,Abd El-Rahman SS,Ata NS

更新日期：2021-02-01 00:00:00

abstract：:In contrast to extensive studies on the roles of molecular chaperones, such as heat shock proteins, there are only a few reports about the roles of GRP78/BiP, an endoplasmic reticulum (ER) stress-induced molecular chaperone, in mammalian cell responses to DNA-damaging stresses. To investigate whether GRP78/BiP is invo...

journal_title：Experimental cell research

authors： Zhai L,Kita K,Wano C,Wu Y,Sugaya S,Suzuki N

更新日期：2005-05-01 00:00:00

abstract：:Serelaxin, a recombinant form of human relaxin-2, is currently regarded as a novel drug for treatment of acute heart failure. However, whether therapeutic effects of serelaxin are achieved by inhibiting cardiac fibrosis remains unclear. In this study, we investigate effects of serelaxin on inhibiting cardiac fibrosis....

journal_title：Experimental cell research

authors： Wu XP,Wang HJ,Wang YL,Shen HR,Tan YZ

更新日期：2018-01-01 00:00:00

abstract：:Human skin fibroblasts deficient in peroxisome biogenesis were transformed by transfecting SV40 ori- DNA with the use of an electroporator, and the biochemical, immunocytochemical, and cytogenetic properties of the transformants were analyzed. Cells (1 x 10(6)) from a patient with Zellweger syndrome and one with neona...

journal_title：Experimental cell research

authors： Okamoto H,Suzuki Y,Shimozawa N,Yajima S,Masuno M,Orii T

更新日期：1992-08-01 00:00:00

abstract：:Telomere shortening in human liver with aging and chronic inflammation was examined by hybridization protection assay using telomere and Alu probes. The reduction rate of telomere repeats in normal liver (23 samples from patients 17-81 years old) was 120 bp per year, which is in good agreement with the reported reduct...

journal_title：Experimental cell research

authors： Aikata H,Takaishi H,Kawakami Y,Takahashi S,Kitamoto M,Nakanishi T,Nakamura Y,Shimamoto F,Kajiyama G,Ide T

更新日期：2000-05-01 00:00:00

abstract：:Carbonic anhydrase II (CA II) expression in characteristic for the early stage of osteoclast differentiation. To study how CA II, which is crucial in proton generation in mature osteoclasts, influences the osteoclast differentiation process we performed rat bone marrow cultures. In this model, acetazolamide, a specifi...

journal_title：Experimental cell research

authors： Lehenkari P,Hentunen TA,Laitala-Leinonen T,Tuukkanen J,Väänänen HK

更新日期：1998-07-10 00:00:00

abstract：:Pair formation in the hypotrichous ciliate Euplotes octocarinatus is a poorly understood phenomenon. In order to obtain information about the molecules involved in this process, we compared ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells. Detection of g...

journal_title：Experimental cell research

authors： Plümper E,Freiburg M,Heckmann K

更新日期：1995-04-01 00:00:00

abstract：:In many eukaryotic organisms, initiation of DNA synthesis is associated with a major control point within the cell cycle and reflects the commitment of the cell to the DNA replication-division portion of the cell cycle. In Paramecium, the timing of DNA synthesis initiation is established prior to fission during the pr...

journal_title：Experimental cell research

authors： Ching AS,Berger JD

更新日期：1986-11-01 00:00:00

abstract：:Skeletal muscle-derived CD34+/45- (Sk-34) cells were identified as a new candidate for stem cells. However, the relationship between Sk-34 cells and side-population (SP) cells is unknown. Here, we demonstrate that Sk-34 cells prepared from murine skeletal muscles consist wholly of main-population (MP) cells. The Sk-34...

journal_title：Experimental cell research

authors： Tamaki T,Akatsuka A,Okada Y,Matsuzaki Y,Okano H,Kimura M

更新日期：2003-11-15 00:00:00

abstract：:Cytoplasts derived from senescent and quiescent human diploid cells inhibit DNA synthesis initiation when fused with cells capable of proliferation. When the cytoplasts were subjected to a variety of conditions (trypsin and cycloheximide treatment and growth on fibronectin), this inhibitory activity was lost, suggesti...

journal_title：Experimental cell research

authors： Pereira-Smith OM,Fisher SF,Smith JR

更新日期：1985-10-01 00:00:00

abstract：:The aging proteostasis decline manifests in a failure of aging cells and organisms to properly respond to proteotoxic challenges. This proteostasis collapse has long been considered a hallmark of aging in nematodes, and has recently been shown to occur also in human cells upon entry to senescence, opening the way to e...

journal_title：Experimental cell research

authors： Meller A,Shalgi R

更新日期：2021-01-09 00:00:00

abstract：:Cystatins constitute a superfamily of cysteine protease inhibitors. A member of the type II secreted cystatin family, cystatin F, has been identified through different gene array experiments to be specifically expressed in hematopoietic cells as well as to be associated with several malignant tumors, suggesting a role...

journal_title：Experimental cell research

authors： Cappello F,Gatti E,Camossetto V,David A,Lelouard H,Pierre P

更新日期：2004-07-15 00:00:00

abstract：:When exponentially growing NHIK 3025 cells were shifted from medium containing 30% serum to medium containing 0.03% serum the rate of net protein accumulation was reduced due to both a reduction in the rate of protein synthesis and an increase in the rate of protein degradation. This change in growth conditions increa...

journal_title：Experimental cell research

authors： Rønning OW,Pettersen EO

更新日期：1984-11-01 00:00:00

abstract：:Electrospinning permits fabrication of biodegradable matrices that can resemble the both scale and mechanical behavior of the native extracellular matrix. However, achieving high-cellular density and infiltration of cells within matrices with traditional technique remain challenging and time consuming. The cell electr...

journal_title：Experimental cell research

authors： Chen H,Liu Y,Hu Q

更新日期：2015-11-01 00:00:00