Abstract:
:Invasiveness and metastatic potential are the two most important properties defining malignancy. The adeno-virus E1A (Ad-E1A) gene has a dual effect as a proliferative gene and as a tumor-suppressor gene, decreasing tumor growth and the metastatic potential of malignant cells. In order to study genes related with the antimetastatic effect of Ad-E1A in human cells, we performed a microarray analysis using OncoChiptrade mark. In three independent experiments, NIH3T3, IMR90 and MDA MB 435 cells were infected with pLPC retroviruses carrying the adenovirus 12S E1A gene or the GFP gene. We analyzed cDNA expression by using the CNIO OncoChipTM, a cDNA microarray containing a total of 6386 genes represented by 7237 clones. uPA, uPAr, tPA, PAI-1 and PAI-2 were also studied at RNA and protein levels. Microarrays of cDNA expression, RT-PCR and Western blot performed in IMR90 E1A-expressing cells showed downregulation of uPA, uPAr, tPA, PAI-1 and upregulation of PAI-2. These results were confirmed in NIH3T3 and MDA MB 435 breast carcinoma cells, with PAI-2 upregulation by RT-PCR and Western blot. In addition, zymographic analysis demonstrated that E1A expression greatly reduced the gelatinase activity of the pro-MMP2 and -MMP9 proteins. We propose that adenovirus E1A may orchestrate the expression of most members of the urokinase-plasminogen activation system, downregulating potentially invasive genes and upregulating PAI-2, which is associated with a better prognosis in human tumors.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Fernández-Soria V,Lleonart ME,Diaz-Fuertes M,Villuendas R,Sánchez-Prieto R,Fabra A,Ramón Y Cajal Skeywords:
subject
Has Abstractpub_date
2006-01-01 00:00:00pages
143-8issue
1eissn
1019-6439issn
1791-2423journal_volume
28pub_type
杂志文章abstract::We have previously shown that the combination of allogeneic intra-bone marrow-bone marrow transplantation (IBM-BMT) and donor lymphocyte infusion (DLI) using CD4+ cell-depleted spleen cells is effective in suppressing tumor growth, but that this does not induce graft-versus-host disease (GVHD) in mice. In this report,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::Lumican is a member of a small leucine-rich proteoglycan family and is highly expressed in several types of cancer cells and/or stromal tissue. Lumican expression in the cytoplasm in advanced colorectal cancer correlates with poor patient prognosis. The expression of lumican in stromal tissues is associated with a hig...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-12-01 00:00:00
abstract::Resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a major limitation for its clinical use. The mechanisms of TRAIL resistance have been mostly studied in the context of cell lines that are intrinsically resistant to TRAIL. However, little is known about the molecular alterations that con...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1748
更新日期:2013-02-01 00:00:00
abstract::The purpose of the study was to assess response rate, clinical outcome, organ/function preservation and toxicity in head and neck cancer patients treated with induction chemotherapy followed by concomitant chemoradiotherapy and, when necessary, limited surgery. The study design was a phase II non-randomized trial in h...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:10.3892/ijo.16.6.1227
更新日期:2000-06-01 00:00:00
abstract::Colorectal cancer (CRC) is one of the most common cancers; however, the development of drugs to treat the condition has reached a plateau. Bortezomib (PS-341, Velcade®) is a proteasome inhibitor approved for the treatment of hematological malignancies, including multiple myeloma. A few trials of bortezomib, alone or i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1448
更新日期:2012-07-01 00:00:00
abstract::The Drosophila ras2 and rop gene pair are governed by a common promoter. Characterisation of the Drosophila ras2/rop bidirectional promoter has revealed that a single major protein complex (M), composed of two subunits (factors A and B) interacts with two central promoter domains (regions A and B). To define the trans...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.5.1185
更新日期:1995-11-01 00:00:00
abstract::In a retrospective analysis of 91 consecutive oral carcinomas we reviewed all patient records and weighed clinicopathological parameters against the results of image DNA cytometry and p53 immunhistochemical staining of the initial diagnostic biopsies. Eighty-seven percent of the biopsies were either aneuploid or nondi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.4.915
更新日期:1994-10-01 00:00:00
abstract::One hundred and eighty-one breast cancer specimens were analyzed for nuclear p53 staining by immunochemical methods. There were 123 fine-needle cytological specimens and 58 frozen tissue sections of surgical biopsies. The microscopic evaluation of the staining fitted with a 4 group classification. Ninety-one samples (...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.4.747
更新日期:1997-04-01 00:00:00
abstract::miRNA‑223 (miR‑223) has been reported to function not only as a tumor suppressor, but also as an oncogenic microRNA (miRNA or miR) in various cancer cells. Therefore, the functional role of miR‑223 has not been elucidated to date, at least to the best of our knowledge. We previously performed the deep sequencing analy...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4762
更新日期:2019-06-01 00:00:00
abstract::PTEN, a gene encoding a dual specificity phosphatase, is frequently altered in endometrial carcinoma. Moreover, these alterations are observed even in atypical hyperplasia of the endometrium. This evidence suggests that mutation of PTEN is an early genetic alteration involved in endometrial carcinogenesis. Adenovirus-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.15.6.1069
更新日期:1999-12-01 00:00:00
abstract::All p53 family members are expressed in several isoforms through alternative promoters and alternative splicing. However, the significance of these isoforms is not yet well understood in cholangiocarcinoma (CCA). In this study, we investigated the expression of p53, p63, p73 and their isoforms at the mRNA and protein ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1818
更新日期:2013-04-01 00:00:00
abstract::Breast cancer (BC) is the most frequently malignancy in women. Therefore, establishment of an animal model for the development of preventative measures and effective treatment for tumors is required. A novel heterogeneous spontaneous mammary tumor animal model of Kunming mice was generated. The purpose of this study w...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2657
更新日期:2014-12-01 00:00:00
abstract::We examined the effects of tranilast on tumor angiogenesis, tumor growth and metastasis in the mouse Lewis lung carcinoma and C57BL mouse system. Tranilast significantly reduced the dense capillary network induced by Lewis lung cancer cells in a mouse dorsal air sac angiogenesis model. Intraperitoneal administration o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.6.1151
更新日期:2000-12-01 00:00:00
abstract::There is increasing evidence that different types of breast cancers are related to distinct risk factors. We analyzed the risk of breast cancer with respect to circulating insulin-like growth factor (IGF)-I, IGF-binding protein (IGFBP)-3, 17beta-estradiol, estrone, testosterone, androstenedione and sex hormone-binding...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::The Drosophila argos protein is a natural ligand antagonist of Drosophila EGF receptor (DER), but does not interact with the human EGF receptor (EGFR). With a view to generating a corresponding EGFR antagonist, we, have designed five chimeric proteins featuring selected characteristic parts of argos within the framewo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.4.677
更新日期:1997-04-01 00:00:00
abstract::Peyronie's disease (PD) is fibrosis localized in the tunica albuginea that is characterized by penile deformity and curvature. The pathogenesis of this disease remains unclear even though transforming growth factor-β (TGF-β)/smad signalling has been reported to be associated with PD. Recent studies have shown that pho...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1905
更新日期:2013-06-01 00:00:00
abstract::Thirty-four children with newly diagnosed acute nonlymphoblastic leukemia were analysed for the expression of P-glycoprotein (P-170), glutathione S-transferase-pi, (GST-pi) topoisomerase II (Topo II) and the proliferation antigen Ki-67 by the streptavidin-biotin-peroxidase method. Overexpression of P-170 was present i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.4.841
更新日期:1994-10-01 00:00:00
abstract::Dexamethasone is a synthetic glucocorticoid frequently used to suppress side-effects of anticancer chemotherapy. In the present study, we showed that dexamethasone treatment leads to concentration-dependent downregulation of cancer-associated marker, carbonic anhydrase IX (CA IX), at the level of promoter activity, mR...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3621
更新日期:2016-10-01 00:00:00
abstract::We previously reported that podophyllotoxin acetate (PA) radiosensitizes NCI-H460 cells. Here, we confirmed that PA treatment also induces cell death among two other non-small cell lung cancer (NSCLC) cell lines: NCI-H1299 and A549 cells (IC50 values = 7.6 and 16.1 nM, respectively). Our experiments further showed tha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3123
更新日期:2015-10-01 00:00:00
abstract::A long-held central dogma of radiation biology has been that the carcinogenic effects of ionizing radiation (IR) are induced by the direct and radiolytic actions of IR on nuclear DNA. Numerous investigations, however, have revealed that several cancer relevant effects of IR can occur in cells that have received only c...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2002-08-01 00:00:00
abstract::Photodynamic therapy (PDT) is a tool against neoplastic and non-neoplastic diseases. PDT is capable to induce different cell death mechanisms in vitro, triggered in a dose-dependent manner. Relationships between PDT and apoptosis or necrosis induction are well-known, but other cell death mechanisms triggered after PDT...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_32_6_1189
更新日期:2008-06-01 00:00:00
abstract::The present study shows that an application of cyclophosphamide (CY) supported by dendritic cell (DC)-based vaccines affected differentiation of the activity of CD4+ T cell subpopulations accompanied by an alteration in CD8+ cell number. Vaccines were composed of bone marrow-derived DCs activated with tumor cell lysat...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3278
更新日期:2016-02-01 00:00:00
abstract::The two-kringle domain of tissue-type plasminogen activator (TK1-2) has been identified as a novel angiogenesis inhibitor. In the previous study, purified Pichia-derived TK1-2 has been shown to suppress in vivo growth of human lung and colon cancer cells. Here, we demonstrate that E. coli-derived non-glycosylated TK1-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-02-01 00:00:00
abstract::Sodium metaarsenite (NaAs2O3: code name KML001) is an orally bioavailable arsenic compound with potential anti-cancer activity. However, the effect of KML001 has not been studied in acute myeloid leukemia (AML). We investigated the anti-leukemic effect of KML001 in AML, and determined the mode of action of KML001. KML...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2899
更新日期:2015-05-01 00:00:00
abstract::The human immunodeficiency virus (HIV) protein negative factor (Nef) is important for AIDS pathogenesis. An anti-Nef single-domain antibody (sdAb19) derived from camelids has been previously generated and shown to effectively block the physiological functions of Nef in vitro and in vivo in nef-transgenic mice. However...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3132
更新日期:2015-10-01 00:00:00
abstract::Meningiomas are benign brain tumors thought to arise by multi-step tumorigenesis, involving both the activation of oncogenes and the loss of tumor suppressor genes. The cell cycle regulator proto-oncogene cyclin D1 has been implicated in the pathogenesis of several types of cancer. Northern blot analysis revealed expr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.9.6.1213
更新日期:1996-12-01 00:00:00
abstract::Patients with long-standing ulcerative colitis (UC) have higher risk of developing colorectal cancer. Albeit the causes remain to be understood, epigenetic alterations have been suggested to play a role in the long-term cancer risk of these patients. In this work, we developed a novel microarray platform based on meth...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1283
更新日期:2012-04-01 00:00:00
abstract::The aim of the present study was to evaluate the performance of whole-body diffusion-weighted imaging (WB-DWI), whole-body positron emission tomography with computed tomography (WB-PET/CT), and whole-body positron emission tomography with magnetic resonance imaging (WB-PET/MRI) in staging patients with untreated invas...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4012
更新日期:2017-07-01 00:00:00
abstract::Ninety-four human non-small cell lung carcinomas (NSCLC) of previously untreated patients were analyzed for the presence of metallothionein (MT) expression by means of immunohistochemistry. Of the tumors investigated, expression of MT was detected in 63%. The expression of MT was correlated with the results of doxorub...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.1.6.687
更新日期:1992-11-01 00:00:00
abstract::Oxygen tension measurements were made in three tumors: (i) the murine FSaII fibrosarcoma, (ii) the rat 9L gliosarcoma and (iii) the rat 13672 mammary adenocarcinoma using a pO2 histograph. Tumor oxygenation measurements were made while the animals breathed air or breathed carbogen (95% oxygen/5% carbon dioxide). Pento...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.1.13
更新日期:1993-01-01 00:00:00