Abstract:
AIM:To establish the rapid, specific, and sensitive method for detecting O157:H7 with DNA microchips. METHODS:Specific oligonucleotide probes (26-28 nt) of bacterial antigenic and virulent genes of E. coli O157:H7 and other related pathogen genes were pre-synthesized and immobilized on a solid support to make microchips. The four genes encoding O157 somatic antigen (rfbE), H7 flagellar antigen (fliC) and toxins (SLT1, SLT2) were monitored by multiplex PCR with four pairs of specific primers. Fluorescence-Cy3 labeled samples for hybridization were generated by PCR with Cy3-labeled single prime. Hybridization was performed for 60 min at 45 degrees. Microchip images were taken using a confocal fluorescent scanner. RESULTS:Twelve different bacterial strains were detected with various combinations of four virulent genes. All the O157:H7 strains yielded positive results by multiplex PCR. The size of the PCR products generated with these primers varied from 210 to 678 bp. All the rfbE/fliC/SLT1/SLT2 probes specifically recognized Cy3-labeled fluorescent samples from O157:H7 strains, or strains containing O157 and H7 genes. No cross hybridization of O157:H7 fluorescent samples occurred in other probes. Non-O157:H7 pathogens failed to yield any signal under comparable conditions. If the Cy3-labeled fluorescent product of O157 single PCR was diluted 50-fold, no signal was found in agarose gel electrophoresis, but a positive signal was found in microarray hybridization. CONCLUSION:Microarray analysis of O157:H7 is a rapid, specific, and efficient method for identification and detection of bacterial pathogens.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Jin HY,Tao KH,Li YX,Li FQ,Li SQdoi
10.3748/wjg.v11.i37.5811keywords:
subject
Has Abstractpub_date
2005-10-07 00:00:00pages
5811-5issue
37eissn
1007-9327issn
2219-2840journal_volume
11pub_type
杂志文章abstract::Portal hypertension, one of the vascular diseases, not only has lesions in liver, but also changes in vascular structures and functions of extrahepatic portal system, systemic system and pulmonary circulation. The pathological changes of vasculopathy in portal hypertension include remodeling of arterialized visceral v...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v11.i39.6079
更新日期:2005-10-21 00:00:00
abstract:AIM:To determine whether intra-arterial infusion of triolein emulsion has biochemical and histopathologic effect on rabbit liver. METHODS:An emulsion of 0.2 mL triolein in 20 mL of saline was infused into either the hepatic arteries of nine rabbits (group 1) or the superior mesenteric arteries of 12 rabbits (group 2)....
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i39.14442
更新日期:2014-10-21 00:00:00
abstract:AIM:To study whether H pylori are associated with chronic cholecystitis. METHODS:The subjects were divided into three groups: H pylori-infected cholecystitis group, H pylori-negative cholecystitis group and control group. Pathologic changes of the gallbladder were observed by optic and electronic microscopes and the l...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i7.1119
更新日期:2007-02-21 00:00:00
abstract:AIM:To investigate the location alteration of Smad2 and Smad4 mRNAs in the liver during and after fibrogenesis in rats. METHODS:Eighty male Wistar rats weighing approximately 200 g each were used. The rat models of experimental hepatic fibrosis were established by injection with carbon tetrachloride (CCl4), normal rat...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i10.1577
更新日期:2006-03-14 00:00:00
abstract::Perfusion of individual tissues is a basic physiological process that is necessary to sustain oxygenation and nutrition at a cellular level. Ischemia, or the insufficiency of perfusion, is a common mechanism for tissue death or degeneration, and at a lower threshold, a mechanism for the generation of sensory signallin...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.15.198
更新日期:2009-01-14 00:00:00
abstract:AIM:To determine whether oral glutathione (GSH) administration can alleviate the effects of fasting-induced intestinal atrophy in the small intestinal mucosa. METHODS:Rats were divided into eight groups. One group was fed ad libitum, another was fed ad libitum and received oral GSH, and six groups were administrated s...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v23.i36.6650
更新日期:2017-09-28 00:00:00
abstract:AIM:To evaluate the expressions of apoptotic signal proteins FADD, TRADD, FasL, Fas, and NFkappaB in gastric carcinoma tissues and their clinical significance. METHODS:Western blot immune trace method was adopted to detect the expressions of apoptotic signal proteins FADD, TRADD, FasL, Fas, and NFkappaB in 55 tissue s...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i25.3846
更新日期:2005-07-07 00:00:00
abstract:AIM:To clarify the molecular mechanism involved in pathogenesis of colorectal cancer as well as clinical significance of genetic analysis of histological samples. METHODS:A total of 480 blood and tissue specimens were collected in our hospital from January 2011 to October 2012. In the observation group, there were 120...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i48.18338
更新日期:2014-12-28 00:00:00
abstract:AIM:To analyze the association of tobacco smoking polymorphism of CYP1A1 (7th exon) and GSTM1 genotype and esophageal cancer(EC) in Xi'an. METHODS:A hospital based case-control study, with molecular epidemiological method, was carried out. Polymorphism of CYP1A1 and GSTM1 of samples from 127 EC cases and 101 controls ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v8.i1.49
更新日期:2002-02-01 00:00:00
abstract::Idiopathic achalasia is a rare primary motility disorder of the esophagus. The classical features are incomplete relaxation of a frequently hypertensive lower esophageal sphincter (LES) and a lack of peristalsis in the tubular esophagus. These motor abnormalities lead to dysphagia, stasis, regurgitation, weight loss, ...
journal_title:World journal of gastroenterology
pub_type: 社论
doi:10.3748/wjg.15.3969
更新日期:2009-08-28 00:00:00
abstract:AIM:To assess B1a cell expression in the rectal mucosa of ulcerative colitis (UC) patients in comparison with healthy controls. METHODS:Rectal mucosa biopsies were collected from 15 UC patients and 17 healthy controls. CD5(+) B cells were analysed by three colour flow cytometry from rectal mucosal samples after mechan...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i2.144
更新日期:2012-01-14 00:00:00
abstract:AIM:To investigate survival rate and incidence of hepatocellular carcinoma (HCC) in patients with decompensated cirrhosis in the antiviral era. METHODS:We used the Korean Health Insurance Review and Assessment. Korea's health insurance system is a public single-payer system. The study population consisted of 286871 pa...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v24.i40.4606
更新日期:2018-10-28 00:00:00
abstract::An insufficient future liver remnant (FLR) is associated with post-hepatectomy liver failure. Associating liver partition and portal vein ligation for stage hepatectomy (ALPPS) has been shown to be effective for the induction of rapid FLR hypertrophy so as to improve the resectability in patients with insufficient FLR...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i29.10208
更新日期:2014-08-07 00:00:00
abstract:BACKGROUND:Colorectal cancer (CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2 (COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and st...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v25.i4.433
更新日期:2019-01-28 00:00:00
abstract:AIM:To identify the predictive clinicopathological factors for lymph node metastasis (LNM) in poorly differentiated early gastric cancer (EGC) and to further expand the possibility of using endoscopic mucosal resection (EMR) for the treatment of poorly differentiated EGC. METHODS:Data were collected from 85 poorly-dif...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.4222
更新日期:2008-07-14 00:00:00
abstract::The major type of human liver cancer is hepatocellular carcinoma (HCC), and there are currently many risk factors that contribute to this deadly disease. The majority of HCC occurrences are associated with chronic hepatitis viral infection, and hepatitis B viral (HBV) infection is currently a major health problem in E...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v21.i42.12157
更新日期:2015-11-14 00:00:00
abstract:AIM:Trace elements (TE) metabolism is altered in inflammatory bowel diseases. TE (zinc and copper) are constituents of antioxidant enzymes. Iron is involved in the pathogenesis of chronic inflammation. The aim was to evaluate zinc and copper status and the effects of iron manipulation in experimental colitis. METHODS:...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i28.4396
更新日期:2005-07-28 00:00:00
abstract:AIM:To identify the susceptible gene (s) for type 2 diabetes in the previously mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes. METHODS:Twenty three SNPs located in 10 candidate genes i...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i9.2078
更新日期:2003-09-01 00:00:00
abstract:AIM:To evaluate the risk factors-other than nonsteroidal anti-inflammatory drugs-for colonic diverticular bleeding in a westernized population. METHODS:One hundred and forty patients, treated for symptomatic diverticular disease in a community based hospital, were included. Thirty (21%) had signs of diverticular bleed...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.457
更新日期:2009-01-28 00:00:00
abstract::Inflammatory bowel diseases (IBDs) are characterized by a chronic course with an alternation of relapses and remissions. Questions about prognosis are important for the patient who wants to know how the disease will affect his/her life and also for clinicians to make management decisions. Correct selection of the pati...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v18.i29.3800
更新日期:2012-08-07 00:00:00
abstract:AIM:To evaluate the efficacy of furazolidone-based triple and quadruple therapy in eradicating Helicobacter pylori (H. pylori) in a multi-center randomized controlled trial. METHODS:A total of 720 H. pylori positive patients with duodenal ulcer disease were enrolled at 10 different hospitals in Jiangxi province in Chi...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3748/wjg.v20.i32.11415
更新日期:2014-08-28 00:00:00
abstract:AIM:To analyze the role of rs12979860 and rs8099917 polymorphisms in hepatitis C virus (HCV) genotype 1 infection of Brazilians. METHODS:A total of 145 adult patients diagnosed with genotype 1 chronic hepatitis C (CHC) who had completed a 48-wk regimen of pegylated-interferon α-2a or -2b plus ribavirin combination the...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.3748/wjg.v19.i42.7399
更新日期:2013-11-14 00:00:00
abstract::The normal esophageal mucosa creates a protective epithelial barrier that constrains the acidic reflux in the esophageal lumen. Microscopic findings and functional studies indicate that this barrier might be impaired in patients with non erosive reflux disease (NERD) but not in patients with functional heartburn (FH)....
journal_title:World journal of gastroenterology
pub_type: 社论,评审
doi:10.3748/wjg.v21.i19.5762
更新日期:2015-05-21 00:00:00
abstract:BACKGROUND:Enterotoxigenic Bacteroides fragilis (ETBF) causes colitis and diarrhea, and is considered a candidate pathogen in inflammatory bowel diseases as well as colorectal cancers. These diseases are dependent on ETBF-secreted toxin (BFT). Dendritic cells (DCs) play an important role in directing the nature of adap...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i3.291
更新日期:2020-01-21 00:00:00
abstract:AIM:To analyze the effect of intralesional steroid injections in addition to endoscopic dilation of benign refractory esophageal strictures. METHODS:A comprehensive search was performed in three databases from inception to 10 April 2017 to identify trials, comparing the efficacy of endoscopic dilation to dilation comb...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,meta分析
doi:10.3748/wjg.v24.i21.2311
更新日期:2018-06-07 00:00:00
abstract:AIM:To evaluate the effects of diazoxide on ischemia/reperfusion (I/R)-injured hepatocytes and further elucidate its underlying mechanisms. METHODS:Male Sprague-Dawley rats were randomized (8 for donor and recipient per group) into five groups: I/R group (4 h of liver cold ischemia followed by 6 h of reperfusion); hem...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i15.1765
更新日期:2012-04-21 00:00:00
abstract:AIM:The use of low-dose aspirin to prevent cardiovascular disease events is well established. However, the incidence and predictors of upper gastrointestinal bleeding (UGIB) with its use are unknown. We studied prospectively the incidence and outcome of peptic ulceration in low-dose aspirin users. METHODS:A total of 9...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i18.2923
更新日期:2006-05-14 00:00:00
abstract:AIM:To analyse the role of genetic susceptibility and environmental factors in the process of esophageal cancer (EC) formation in Xi'an, China. METHODS:A hospital based case-control study, combined with molecular epidemiological method, was carried out. A total of 127 EC cases and 101 controls were interviewed with qu...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v10.i7.940
更新日期:2004-04-01 00:00:00
abstract::We describe herein a 68-year-old woman who was diagnosed with a quite rare entity of intraductal papillary mucinous neoplasms (IPMNs) occurring simultaneously in the left lateral lobe of liver and the tail of pancreas. Abdominal computed tomography and magnetic resonance cholangiopancreatography showed a cystic dilata...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i44.6510
更新日期:2012-11-28 00:00:00
abstract::INTRODUCTION:To intrestigate the relationship between tumor necrosis factor-alphaand liver fibrosis in patients with chronic liver disease.METHODS:Radioimmunoassay was made in 20 patients with mild chronic hepatitis(CMH),20 patients with severe chronic hepatitis(CSH),51 patients with liver cirrhosis(LC)and 32-normal p...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v4.i1.18
更新日期:1998-02-01 00:00:00