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Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells.

Abstract：

OBJECTIVE:To evaluate the transcription and translation ability of mitochondria in terminally differentiated granulosa cells, these cells were incubated with ionic calcium. DESIGN:Prospective laboratory research. SETTING:In vitro fertilization laboratory in a university hospital. PATIENT(S):Granulosa cells were harvested from 50 female patients undergoing IVF. INTERVENTION(S):Analysis of mitochondrial gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and of mitochondrial-encoded proteins by Western blot. MAIN OUTCOME MEASURE(S):Comparison of the RNA expression levels of genes including cytochrome c oxidase subunit I (COX I), adenosine triphosphate synthase 6 (ATPase 6), flavoprotein, and succinate-ubiquinone oxidoreductase, and protein levels of COX I and flavoprotein in different calcium ion treatment groups. RESULT(S):There were dose-dependent increases in RNA expressions of the four genes analyzed from granulosa cells cultured in a serial concentration of calcium ions. This effect was abolished when cells were preincubated with the extracellular calcium-chelating agent, Ethylene glycol-bis (2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA). The effect of ionic calcium on both the nuclear- and mitochondrial-encoded subunits also was determined. Expression levels of mitochondrial transcription factor A in RNA significantly increased in granulosa cells that were exposed for 24 and 48 hours to 0.5 and 1 microM A23187. CONCLUSION(S):The present study is the first report to present calcium-dependent increases in the transcription and translation levels of both nuclear-encoded and mitochondrial-encoded mitochondrial respiratory enzyme subunits and also indicates that mitochondrial transcription factor A is involved in mitochondrial biogenesis.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Au HK,Yeh TS,Kao SH,Shih CM,Hsieh RH,Tzeng CR

doi

10.1016/j.fertnstert.2005.03.072

keywords:

subject

Has Abstract

pub_date

2005-10-01 00:00:00

pages

1104-8

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(05)01297-5

journal_volume

84 Suppl 2

pub_type

杂志文章

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Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells.