Crystallization and preliminary diffraction studies of hydroxypyruvate reductase (D-glycerate dehydrogenase) from Hyphomicrobium methylovorum.

abstract：:We propose a general classification scheme for loops, aperiodic segments of protein structure. In an effort to avoid the geometric complexity created by non-repeating phi psi angles, a morphologic definition that focuses upon the linearity and planarity of loops is utilized. Out of 432 loops (4 to 20 residues in lengt...

journal_title：Journal of molecular biology

authors： Ring CS,Kneller DG,Langridge R,Cohen FE

更新日期：1992-04-05 00:00:00

abstract：:The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints ...

journal_title：Journal of molecular biology

authors： Schultze P,Wörgötter E,Braun W,Wagner G,Vasák M,Kägi JH,Wüthrich K

更新日期：1988-09-05 00:00:00

abstract：:HscA, a specialized bacterial Hsp70-class molecular chaperone, interacts with the iron-sulfur cluster assembly protein IscU by recognizing a conserved LPPVK sequence motif. We report the crystal structure of the substrate-binding domain of HscA (SBD, residues 389-616) from Escherichia coli bound to an IscU-derived pep...

journal_title：Journal of molecular biology

authors： Cupp-Vickery JR,Peterson JC,Ta DT,Vickery LE

更新日期：2004-09-24 00:00:00

abstract：:We describe the physicochemical characterization of various circular and linear forms of the approximately 60 residue N-terminal Src homology 3 (SH3) domain from the murine c-Crk adapter protein. Structural, dynamic, thermodynamic, kinetic and biochemical studies reveal that backbone circularization does not prevent t...

journal_title：Journal of molecular biology

authors： Camarero JA,Fushman D,Sato S,Giriat I,Cowburn D,Raleigh DP,Muir TW

更新日期：2001-05-18 00:00:00

abstract：:Bacteriophage P4 immunity is controlled by a small stable RNA (CI RNA) that derives from the processing of primary transcripts. In previous works, we observed that the endonuclease RNase P is required for the maturation of CI RNA 5'-end; moreover, we found that polynucleotide phosphorylase (PNPase), a 3' to 5' RNA-deg...

journal_title：Journal of molecular biology

authors： Briani F,Del Vecchio E,Migliorini D,Hajnsdorf E,Régnier P,Ghisotti D,Dehò G

更新日期：2002-04-26 00:00:00

abstract：:The humoral immune system in higher vertebrates is unique in its ability to generate highly diverse antibody responses against most pathogens as well as against certain malignancies. Several technologies have been developed to exploit this vast source of potentially therapeutic antibodies, including hybridoma technolo...

journal_title：Journal of molecular biology

authors： Meijer PJ,Andersen PS,Haahr Hansen M,Steinaa L,Jensen A,Lantto J,Oleksiewicz MB,Tengbjerg K,Poulsen TR,Coljee VW,Bregenholt S,Haurum JS,Nielsen LS

更新日期：2006-05-05 00:00:00

abstract：:Azaleucine is a naturally occurring amino acid antibiotic that can be incorporated into proteins by mimicking leucine. In spite of its highly toxic character, the contrast between the hydrophobic side-chain of leucine and the basic side-chain of azaleucine suggested a mechanism for rescuing certain mutants. We constru...

journal_title：Journal of molecular biology

authors： Lemeignan B,Sonigo P,Marlière P

更新日期：1993-05-20 00:00:00

abstract：:Dysregulation of hepatocyte growth factor (HGF)-induced signaling via its receptor tyrosine kinase Met results in tumor progression and metastasis. To initiate signaling, pro-HGF must be proteolytically activated to reveal a secondary Met binding site within the serine protease-like beta-chain of HGF. Although HGF/Met...

journal_title：Journal of molecular biology

authors： Tam EM,Runyon ST,Santell L,Quan C,Yao X,Kirchhofer D,Skelton NJ,Lazarus RA

更新日期：2009-01-09 00:00:00

abstract：:The majority of cell surface receptors involved in antigen recognition by T cells and in the orchestration of the subsequent cell signalling events are glycoproteins. The length of a typical N-linked sugar is comparable with that of an immunoglobulin domain (30 A). Thus, by virtue of their size alone, oligosaccharides...

journal_title：Journal of molecular biology

authors： Rudd PM,Wormald MR,Stanfield RL,Huang M,Mattsson N,Speir JA,DiGennaro JA,Fetrow JS,Dwek RA,Wilson IA

更新日期：1999-10-22 00:00:00

abstract：:In bacteria and lower eukaryotes, adaptation to changes in the environment is often mediated by two-component regulatory systems. Such systems provide the basis for chemotaxis, nitrogen and phosphate regulation and adaptation to osmotic stress, for example. In Escherichia coli, the sensor kinase EnvZ detects a change ...

journal_title：Journal of molecular biology

authors： Tran VK,Oropeza R,Kenney LJ

更新日期：2000-06-23 00:00:00

abstract：:Molecular aspects of thermal adaptation of proteins were studied by following the co-evolution of temperature dependence, conformational stability, and substrate specificity in a cold-active lipase modified via directed evolution. We found that the evolution of kinetic stability was accompanied by a relaxation in subs...

journal_title：Journal of molecular biology

authors： Gatti-Lafranconi P,Natalello A,Rehm S,Doglia SM,Pleiss J,Lotti M

更新日期：2010-01-08 00:00:00

abstract：:While the gene for p53 is mutated in many human cancers causing loss of function, many others maintain a wild-type gene but exhibit reduced p53 tumor suppressor activity through overexpression of the negative regulators, Mdm2 and/or MdmX. For the latter mechanism of loss of function, the activity of endogenous p53 can...

journal_title：Journal of molecular biology

authors： Grace CR,Ban D,Min J,Mayasundari A,Min L,Finch KE,Griffiths L,Bharatham N,Bashford D,Kiplin Guy R,Dyer MA,Kriwacki RW

更新日期：2016-03-27 00:00:00

abstract：:The mammalian Sec61 complex forms a protein translocation channel whose function depends upon its interaction with the ribosome and with membrane proteins of the endoplasmic reticulum (ER). To study these interactions, we determined structures of "native" ribosome-channel complexes derived from ER membranes. We find t...

journal_title：Journal of molecular biology

authors： Ménétret JF,Hegde RS,Heinrich SU,Chandramouli P,Ludtke SJ,Rapoport TA,Akey CW

更新日期：2005-04-29 00:00:00

abstract：:The three-dimensional structure of cytochrome-c552 from Thermus thermophilus has been determined by the multiple anomalous dispersion technique using synchrotron radiation and refined to a resolution of 1.28 A. Data collection at 90 K and the recording of three data sets (f'-minimum: 7125 eV, f"-maximum: 7138 eV and r...

journal_title：Journal of molecular biology

authors： Than ME,Hof P,Huber R,Bourenkov GP,Bartunik HD,Buse G,Soulimane T

更新日期：1997-08-29 00:00:00

abstract：:In-vivo studies have demonstrated that adenovirus type 2 and adenovirus type 4 have different DNA sequence requirements for the initiation of DNA replication. To investigate the basis of these differences an in-vitro system has been developed which will faithfully initiate adenovirus type 4 DNA replication. A plasmid ...

journal_title：Journal of molecular biology

authors： Harris MP,Hay RT

更新日期：1988-05-05 00:00:00

abstract：:An automatic procedure for defining recurrent folding motifs in proteins of known structure is described. These motifs are formed by short polypeptide fragments of equal size containing between four and seven residues. The method applies a classical clustering algorithm that operates on distances between selected back...

journal_title：Journal of molecular biology

authors： Rooman MJ,Rodriguez J,Wodak SJ

更新日期：1990-05-20 00:00:00

abstract：:The loop between the F and G beta strands (FG loop) of the bacteriophage MS2 coat protein subunit forms inter-subunit contacts around the 5-fold and 3-fold (quasi 6-fold) axes of the T=3 protein shell. In capsids, the loop is found in two very different conformations, one in B subunits, which form the 5-fold contact, ...

journal_title：Journal of molecular biology

authors： Stonehouse NJ,Valegård K,Golmohammadi R,van den Worm S,Walton C,Stockley PG,Liljas L

更新日期：1996-02-23 00:00:00

abstract：:Two types of annelid collagens of different sizes were purified, one from acetic acid extracts of the cuticle (length 2.5 microns) and the other, after pepsin digestion, from interstitial spaces of the body wall (0.3 micron). They were obtained from Alvinella pompejana, Alvinella caudata and Paralvinella grasslei coll...

journal_title：Journal of molecular biology

authors： Gaill F,Mann K,Wiedemann H,Engel J,Timpl R

更新日期：1995-02-17 00:00:00

abstract：:The restriction enzyme R.BbvCI cleaves duplex DNA within a seven base-pair asymmetric recognition sequence, thus: CCTCAGC/GCTGAGG-->CC--TCAGC/GC--TGAGG. We show that R.BbvCI comprises two different subunits, R(1) and R(2); that each subunit contains a catalytic site for DNA strand hydrolysis; and that these sites act ...

journal_title：Journal of molecular biology

authors： Heiter DF,Lunnen KD,Wilson GG

更新日期：2005-05-06 00:00:00

abstract：:Allosteric regulatory processes are implicated at all levels of biological function. Recent advances in our understanding of the diverse and functionally significant class of intrinsically disordered proteins have identified a multitude of ways in which disordered proteins function within the confines of the allosteri...

journal_title：Journal of molecular biology

authors： Berlow RB,Dyson HJ,Wright PE

更新日期：2018-08-03 00:00:00

abstract：:Restriction fragments of bacteriophage lambda DNA corresponding to the major leftward transcription unit were purified, denatured to form single-stranded DNA, self-annealed, and examined by electron microscopy. Three intrastrand stem and loop secondary structures were observed reproducibly and the locations of the pai...

journal_title：Journal of molecular biology

authors： Edlind TD,Cooley TE,Richards SH,Ihler GM

更新日期：1984-11-05 00:00:00

abstract：:Recently, the absence of a thermally induced transition has been offered as proof that the unfolded and the molten globule states of apo-alpha-lactalbumin are enthalpically equivalent. In this paper we demonstrate that that argument is thermodynamically incorrect. In addition, it is shown that the absence of a thermal...

journal_title：Journal of molecular biology

authors： Xie D,Bhakuni V,Freire E

更新日期：1993-07-05 00:00:00

abstract：:Here, we describe the complete deduced amino acid sequence of three unconventional myosins identified in the protozoan parasite Toxoplasma gondii. Phylogenetic analysis reveals that the three myosins represent a novel, highly-divergent class addition to the myosin superfamily. Toxoplasma gondii myosin-A (TgM-A) is a r...

journal_title：Journal of molecular biology

authors： Heintzelman MB,Schwartzman JD

更新日期：1997-08-08 00:00:00

abstract：:The essential cell division genes ftsQ, ftsA and ftsZ map in a cluster of cell envelope genes located at two minutes on the Escherichia coli genetic map and appear to constitute an atypical operon. These closely clustered genes are all transcribed in the same direction and yet each of these genes was independently clo...

journal_title：Journal of molecular biology

authors： Yi QM,Rockenbach S,Ward JE Jr,Lutkenhaus J

更新日期：1985-08-05 00:00:00

abstract：:Post-translational modifications are essential for the proper function of many proteins in the cell. The attachment of an isoprenoid lipid (a process termed prenylation) by protein farnesyltransferase (FTase) or geranylgeranyltransferase type I (GGTase-I) is essential for the function of many signal transduction prote...

journal_title：Journal of molecular biology

authors： Reid TS,Terry KL,Casey PJ,Beese LS

更新日期：2004-10-15 00:00:00

abstract：:The transcriptional co-activators CBP/p300 and PCAF participate in transcriptional activation by many factors. We have shown that both CBP/p300 and PCAF stimulate the transcriptional activation by KLF13, a member of the KLF/Sp1 family, either individually or cooperatively. Here we further investigated how CBP and PCAF...

journal_title：Journal of molecular biology

authors： Song CZ,Keller K,Chen Y,Stamatoyannopoulos G

更新日期：2003-05-30 00:00:00

abstract：:Residues specifying aminoacylation by yeast tyrosyl-tRNA synthetase (TyrRS) of the tRNA-like structure present at the 3'-end of brome mosaic virus (BMV) RNA were determined by the in vitro approach using phage T7 transcripts. They correspond to nucleotides equivalent to base-pair C1-G72 and discriminator base A73 in t...

journal_title：Journal of molecular biology

authors： Fechter P,Giegé R,Rudinger-Thirion J

更新日期：2001-06-01 00:00:00

abstract：:Nebulin, a large protein (600 to 800 kDa) located in the thin filament of striated vertebrate muscle, is assumed to bind and stabilise F-actin. Complete sequence determination of human nebulin has only recently been accomplished showing a uniform modular structure along the whole length of the molecule. Up to 97% of t...

journal_title：Journal of molecular biology

authors： Pfuhl M,Winder SJ,Castiglione Morelli MA,Labeit S,Pastore A

更新日期：1996-03-29 00:00:00

abstract：:Members of the double-stranded RNA (dsRNA) specific RNase III family are known to use a conserved dsRNA-binding domain (dsRBD) to distinguish RNA A-form helices from DNA B-form ones, however, the basis of this selectivity and its effect on cleavage specificity remain unknown. Here, we directly examine the molecular re...

journal_title：Journal of molecular biology

authors： Lamontagne B,Hannoush RN,Damha MJ,Abou Elela S

更新日期：2004-04-23 00:00:00

abstract：:Tk-SP is a hyperthermostable subtilisin-like serine protease from Thermococcus kodakaraensis and is autoprocessed from its precursor (Pro-Tk-SP) with N- and C-propeptides. The crystal structure of the active-site mutant of Pro-Tk-SP lacking C-propeptide, ProN-Tk-S359A, was determined at 2.0 A resolution. ProN-Tk-S359A...

journal_title：Journal of molecular biology

authors： Foophow T,Tanaka S,Angkawidjaja C,Koga Y,Takano K,Kanaya S

更新日期：2010-07-23 00:00:00