Abstract：
OBJECTIVE:To investigate the effect of SKI 306X, a purified extract from the mixture of three herbs, i.e. Clematis mandshurica, Trichosanthes kirilowii and Prunella vulgaris, on apoptosis in chondrocytes. DESIGN:Rat chondrocyte cell line RCJ3.1C.18 cells were incubated with 1 microM staurosporin and SKI 306X or each of its components. Cell viability was determined by trypan blue exclusion assay. Induction of apoptosis was determined by nuclear condensation or fragmentation after Hoechst staining. Amount of apoptosis was quantified both by nuclear morphology and flow cytometry. Expression level of Bcl-2, and caspase-3 and PARP activations were assayed by Western blot. RESULTS:SKI 306X significantly prevented staurosporin-induced apoptosis. Among its three components, only Clematis mandshurica significantly decreased the amount of staurosporin-induced apoptosis. Although the level of Bcl-2 expression was decreased after staurosporin treatment, it was sustained after the combination treatment with Clematis mandshurica. Whereas staurosporin induced the degradation of 32 kDa caspase-3 precursor and the production of 85-kDa cleavage products of PARP in a time-dependent fashion, Clematis mandshurica treatment prevented those manifestations. CONCLUSIONS:Pharmacological efficacy of SKI 306X protecting osteoarthritis in part may result from the inhibition of apoptosis in chondrocytes by Clematis mandshurica.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Lee SW,Chung WT,Choi SM,Kim KT,Yoo KS,Yoo YHdoi
10.1016/j.jep.2005.05.009keywords:
subject
Has Abstractpub_date
2005-10-03 00:00:00pages
294-8issue
1-3eissn
0378-8741issn
1872-7573pii
S0378-8741(05)00312-0journal_volume
101pub_type
杂志文章abstract：ETHNOPHARMACOLOGICAL USAGES:Leaves and roots of Pfaffia glomerata areused as aphrodisiacs, tranquilizers and antirheumatics. Due to the lack of experimental scientific data, studies are necessary to identify its medicinal properties. AIMS:The present study aimed to evaluate the effect of hydroalcoholic root extract of...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2018.12.047
更新日期：2019-04-06 00:00:00
abstract：:The present study evaluated the effect of green tea (Camellia sinensis L.) leaf extract on triglyceride and glucose homeostasis in a fructose-fed hypertriglyceridemic, insulin-resistant hamster model. There was a significant decrease in plasma triglyceride levels following supplementation of the green tea epigallocate...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2005.08.045
更新日期：2006-03-08 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:Traditional Chinese herbal medicine (CHM) has a long-history for treatment of various human diseases including tumors, infection, autoimmune diseases in Asian countries, especially in China, Japan, Korea and India. CHM was traditionally used as water extracts and many Chinese herbs were c...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章,评审
doi：10.1016/j.jep.2015.05.050
更新日期：2015-08-02 00:00:00
abstract：:The hexane, chloroform, acetone and methanolic fractions of Datura metel L. were investigated for antifungal properties using pathogenic species of Aspergillus. The chloroform fraction was found to be endowed with antifungal activity. The minimum inhibitory concentration (MIC) of chloroform fraction of D. metel L. was...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/s0378-8741(02)00036-3
更新日期：2002-05-01 00:00:00
abstract：:The anticonvulsant effect of the volatile oil extracted from the fresh fruits of Tetrapleura tetraptera was investigated in mice. The results obtained show that the fresh oil given intraperitoneally offers some protection against leptazol-induced convulsions. A dose of 0.4 ml of the oil per mouse protected 78% of the ...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/0378-8741(86)90023-1
更新日期：1986-11-01 00:00:00
abstract：:This study investigates anticonvulsant effects of an essential oil of the fruits of Pimpinella anisum (Umbelliferae), a folkloric remedy in the Iranian traditional medicine, against seizures induced by pentylenetetrazole (PTZ) or maximal electroshock (MES) in male mice. The essential oil suppressed tonic convulsions i...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/s0378-8741(98)00161-5
更新日期：1999-08-01 00:00:00
abstract：:Pseudo-akuammigine, an alkaloid from Picralima nitida seed extract was investigated for anti-inflammatory and analgesic actions using the carrageenan-induced rat paw oedema and the rat tail flick. The alkaloid, at 1.0, 5.0 and 50 mgkg(-1)(,) dose-dependently inhibited the mean maximal paw swelling attained during 6 h ...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/s0378-8741(02)00058-2
更新日期：2002-06-01 00:00:00
abstract：AIM OF THE STUDY:Zanthoxylum rhoifolium Lam. (Rutaceae) is locally known as "mamica de cadela", and its bark is popularly used for toothache and earache. The objective of this study was to investigate the antinociceptive effect of the ethanolic extract from this species' stem bark (EtOH), its fractions of partition (he...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2010.03.009
更新日期：2010-05-27 00:00:00
abstract：:Thirty four crude extracts of Panamanian plants, from nine species of Celastraceae and Lamiaceae, were assayed for xanthine oxidase (XO) inhibitory activity. The enzymatic activity was estimated by measuring the increase in absorbance at 290 nm due to uric acid formation. Eighty five percent of the crude extracts were...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/0378-8741(95)01225-3
更新日期：1995-04-01 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:The flowers of Hibiscus rosa sinensis has array of pharmacological actions. They are used in preparation of herbal decoction and teas, which have been used traditionally to reduce body weight and for its effect on metabolic syndrome. AIM OF THE STUDY:To investigate the anti adipogenic ef...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2018.12.039
更新日期：2019-04-06 00:00:00
abstract：:Tanzania is endowed with a rich natural resource. The biodiversity comprises over 10,000 plant species, a rich fauna and marine resources. The collection of biological resources is guided by a set of formalities, namely: (i) entry visas, (ii) research permits, (iii) designation of a relevant collaborating host institu...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章,评审
doi：10.1016/0378-8741(95)01365-2
更新日期：1996-04-01 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:Dragon's Blood from Dracaena cochinchinensis (Lour.) S.C. Chen (Yunnan, China), as a traditional Chinese medicinal herb, was shown to have certain antithrombotic effects. A new preparation process was used to extract effective components from Dragon's Blood. A 95% ethanol extract A (EA) a...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2011.03.063
更新日期：2011-05-17 00:00:00
abstract：AIMS OF THE STUDY:The South American mint genus Minthostachys is of great importance in the Andes as a medicinal, aromatic, culinary and commercial essential oil plant. After decades of taxonomic confusion and virtual indeterminability of specimens, new systematic and taxonomic work has been conducted in recent years. ...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2008.05.030
更新日期：2008-08-13 00:00:00
abstract：:Montanoa tomentosa (zoapatle) is a Mexican plant popularly used for antifertility purposes and with promising characteristics for the development of a new oral contraceptive agent with uteroevacuant properties. In a previous pharmacological report we established the ambivalent myometrial response to zoapatle leaves aq...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/0378-8741(84)90098-9
更新日期：1984-06-01 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:In Korea, Morus alba leaves have been traditionally administered as natural therapeutic agent for the alleviating dropsy and diabetes. AIM OF THE STUDY:The present study was performed to evaluate melanin-concentrating hormone receptor subtype 1 (MCH1) antagonism of the ethanol extract of...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2009.01.020
更新日期：2009-03-18 00:00:00
abstract：:Achyrocline satureioides (Lam.) DC (Marcela) is known to possess a broad spectrum of pharmacological, medicinal and therapeutic properties. Previous studies have demonstrated various protective abilities of the marcela extracts against various pathological conditions. However, no extensive safety studies have been con...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2004.08.013
更新日期：2004-12-01 00:00:00
abstract：:The antibacterial activities of the methanol and hot and cold aqueous extracts of the leaves of Aspilia africana, Ficus exasperata and Mareya micrantha were bioassayed against three Gram-negative and three Gram-positive bacterial species: Aerobacter aerogenes, Agrobacterium tumefaciens, Bacillus subtilis, Clostridium ...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/0378-8741(90)90083-6
更新日期：1990-03-01 00:00:00
abstract：:Cedronin was isolated from Simaba cedron Planchon (Simaroubaceae), a species popularly believed in South America to have antimalarial properties. It was examined for in vitro and in vivo antimalarial activities and for cytotoxicity against KB cells. Experimental results showed that cedronin was active against chloroqu...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/0378-8741(94)90117-1
更新日期：1994-06-01 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:The development of selective inhibitors of monoamine oxidase B (MAO-B) has been essential in treating Parkinson's disease. However, the apparent hepatotoxicity and drug-drug interactions of current inhibitors accentuate the need for the development of novel pharmacotherapies. Crossyne gut...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2019.112305
更新日期：2020-02-10 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:Alstonia scholaris (Apocynaceae) was documented as an effective herb for the treatment of chronic respiratory diseases in "dai" ethnopharmacy historically, and its leaf crude extract, used for releasing tracheitis and cold symptom, was approved to be a commercial formulation by State Food...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2010.03.029
更新日期：2010-06-16 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:Curcuma mangga Valeton & Van Zijp. (Zingiberaceae family) contains curcuminoids and diterpenes which have anti-inflammatory effect. In traditional use, the elixir of this plant has been used to detoxify the poisonous plants, treatment of gastric ulcer, chest pain, fever, skin disease and ...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2019.111828
更新日期：2019-06-28 00:00:00
abstract：:Lyophilised aqueous extract of Euphorbia hirta L. (Euphorbiaceae) has been evaluated for behavioral effects in mice. The extract did not induce any toxic effect when it was administered i.p. and orally. Sedative properties could be confirmed with high doses (100 mg of dried plant/kg, and more), by a decrease of behavi...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/0378-8741(90)90055-x
更新日期：1990-05-01 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:The traditional herbal medicine Tripterygium wilfordii Hook. f. (TW) has been widely used for the treatment of rheumatoid arthritis and autoimmune disease in the clinic. However, adverse reactions of TW including hepatotoxicity and nephrotoxicity have been frequently reported. Terpenes an...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2015.01.004
更新日期：2015-03-13 00:00:00
abstract：:From September 1990 to December 1994, the authors conducted an ethnobotanical survey within the population of Martinique. Two hundred and sixty-one plants used for medicinal purposes were gathered. It was possible to identify 251 species (96%). Traditional medicinal practices, although threatened by the modern pharmac...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/0378-8741(96)01425-0
更新日期：1996-09-01 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:Traditional Chinese medicine Yifei Tongluo Granules has been employed clinically with the combination of chemotherapy agents to treat patients with multidrug-resistant tuberculosis. However, the mechanisms underlying the therapeutic potential have not been well elucidated. The present stu...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2016.08.046
更新日期：2016-12-24 00:00:00
abstract：:Oral administration of a CH(2)Cl(2)-MeOH (1:1) extract of Scaphyglottis livida produced dose-dependent antinociceptive and anti-inflammatory effects when tested in mice and rats using the hot-plate (150-600 mg/kg) and carrageenan-induced inflammation (150-600 mg/kg) models, respectively. Morphine (1.5-6 mg/kg, p.o.) a...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2007.07.021
更新日期：2007-11-01 00:00:00
abstract：:The hepatoprotective activity of the rhisome of Cochlospermum tinctorium was investigated using carbon tetrachloride toxicity on mouse and tert-butyl hydroperoxide in vitro induction of lipid peroxidation and hepatocyte lysis. Aqueous, hydro-ethanolic and ethanolic extracts showed significant dose-dependent hepatoprot...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/0378-8741(92)90013-h
更新日期：1992-04-01 00:00:00
abstract：AIM OF THE STUDY:Calotropis procera is a wild growing plant with multifarious medicinal properties. The present study was carried out to evaluate the effect of dried latex (DL) of Calotropis procera and its methanol extract (MeDL) against gastric ulcers induced in rats. MATERIALS AND METHODS:Aqueous suspension of DL (...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2009.10.016
更新日期：2010-02-03 00:00:00
abstract：ETHNOPHARMACOLOGICAL RELEVANCE:Calculus bovis (C. bovis), a widespread known traditional animal drug in China and Japan, has been widely used for a long time to treat various diseases, including high fever, convulsion and stroke. The aim of the present paper is to comprehensively review knowledge about C. bovis in term...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章,评审
doi：10.1016/j.jep.2020.112649
更新日期：2020-05-23 00:00:00
abstract：:Hydroethanol (HE) and methanol (ME) extracts obtained from the leaves of Passiflora actinia Hooker were evaluated for behavioral effects in mice. Single-dose oral administration of HE (300 and 600 mg/kg) or ME (100 and 300 mg/kg) resulted in anxiolytic-like effects in the elevated plus-maze. The anxiolytic-like effect...
journal_title：Journal of ethnopharmacology
pub_type： 杂志文章
doi：10.1016/j.jep.2006.11.021
更新日期：2007-05-04 00:00:00
