Abstract:
:In order to analyse the effects of glucocorticoids on interleukin-8 (IL-8) production more precisely, we examined the effects of dexamethasone on IL-8 production at the molecular level in a human fibrosarcoma cell line, 8387, which IL-1 induces to express IL-8 messenger RNA (mRNA) and to secrete IL-8. Over a wide dose range, dexamethasone inhibited IL-8 production induced by IL-1 alpha stimulation. Northern blotting analysis showed that dexamethasone also inhibited the IL-8 mRNA accumulation in a similar dose-related manner. Nuclear run-off assay revealed that dexamethasone decreased the transcription of the IL-8 gene and the degree of inhibition of transcription correlated well with the inhibition of IL-8 production, suggesting that the action of glucocorticoids is mainly at the transcriptional level. Furthermore, transfection with chloramphenicol acetyl transferase (CAT) expression vectors inserted with the 5'-deleted IL-8 gene demonstrated that the 5'-flanking region which contains the glucocorticoid response element (GRE) was mainly involved in the dexamethasone-induced repression of the IL-8 gene. These data suggest that the inhibition of the IL-8 gene transcription by glucocorticoids occurs through the interaction of the glucocorticoid receptor complex with GRE in the 5'-flanking region of the IL-8 gene.
journal_name
Immunologyjournal_title
Immunologyauthors
Mukaida N,Gussella GL,Kasahara T,Ko Y,Zachariae CO,Kawai T,Matsushima Kkeywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
674-9issue
4eissn
0019-2805issn
1365-2567journal_volume
75pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Electron microscopic study of the events occurring at the cell membrane during reactive lysis by complement, showed that a foliaceous particle was formed at the C5b-7 stage, that enlarged to a particle with a variable number of arms at the C5b-8 stage. Up to this point, no typical complement lesions were found. At the...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-08-01 00:00:00
abstract::Although it is known that dendritic cells (DC) migrate in response to inflammatory stimuli. There is little information about the expression of receptors for chemotactic factors on DC. The present study has demonstrated by double immunostaining and flow cytometry of Langerhan's cell (LC)-enriched epidermal cell suspen...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-701.x
更新日期:1996-09-01 00:00:00
abstract::The CD3-T-cell receptor complex is the clonotypic surface structure by which T lymphocytes recognize foreign antigens and are subsequently activated. Because of the low immunogenicity of the CD3 molecules, anti-CD3 monoclonal antibodies (mAb) are difficult to prepare and have not been available in several species. Fol...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-682.x
更新日期:1996-08-01 00:00:00
abstract::BALB/c mice develop myasthenic symptoms after immunization with rodent acetylcholine receptor (AChR). After immunization with Torpedo AChR (TAChR), their CD4+ cells become strongly sensitized against a conserved region of the TAChR alpha subunit sequence (residues alpha 304-322), and cross-react vigorously with the ho...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Docosahexaenoic (22:6 n-3) and eicosapentaenoic acid (20:5 n-3) stimulated the oxygen-dependent respiratory burst in intact neutrophils in a dose-dependent manner as measured by either superoxide dismutase (SOD)-inhibitable cytochrome c reduction and lucigenin-dependent chemiluminescence. A number of longer chain hexa...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::To evaluate the role of the high-affinity monocyte receptor for lipopolysaccharide (LPS), CD14, in the process of tolerance to LPS, the human monocytic cell line Mono-Mac-6 was cultured in the absence or presence of different amounts of LPS. The kinetics of CD14 modulation in these cells showed an initial 4-day period...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::A murine anti-B monoclonal antibody was obtained by the hybridoma technique. This antibody called anti-B (b-183) is of IgM nature; it is capable of agglutinating normal B, B3, Bx, cis AB and some acquired B red cells. Its association constant is 1.1 X 10(8) l/mol, and appears high compared to those of the monoclonal a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::Montelukast is a leukotriene receptor antagonist that is known to prevent allergic rhinitis and asthma. Blocking the Cysteinyl leukotrienes receptor (CysLTR1), one of the primary receptors of leukotrienes, has been demonstrated to be efficacious in ameliorating experimental autoimmune encephalomyelitis (EAE), an anima...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13308
更新日期:2021-01-22 00:00:00
abstract::Despite the capacity for antigen-specific activation and rapid clonal expansion, homeostatic mechanisms ensure that the mature immune system contains a relatively stable number of T cells. In recent years, it has become apparent that this stability is a consequence of apoptotic death of most of the specific T cells ge...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00048.x
更新日期:2000-07-01 00:00:00
abstract::The immunophenotypes of the HLA-DR-positive leucocyte populations in normal human skin were studied using an extensive panel of monoclonal antibodies, which included antibodies from the Third International Leucocyte Differentiation Antigen Workshop (3rd LDAW). Langerhans' cells (LC) in the epidermis stained with antib...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::Virus plaque assay (VPA) was utilized for the quantitative evaluation of activated lymphocytes. We examined what types of cells, especially which of activated T and non-T lymphocytes, were detected as infective centres after infection with vesicular stomatitis virus. Marked increases in DNA synthesis and in virus-plaq...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract::We aimed to establish a panel of MHC-peptide multimers suitable as a positive control in the detection of HLA A*0201 restricted antigen specific T cells (ASTC) by flow cytometry. MHC Dextramers were loaded with HLA A*0201 binding peptides from viral antigens and melanoma targets identified from a literature search and...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12769
更新日期:2017-10-01 00:00:00
abstract::Mice fed a protein-deficient diet containing 2% ovalbumin for 6 weeks (PD) show a reduced spleen IgA plaque-forming cell (PFC) response after oral immunization with sheep red blood cells (SRBC), compared to the response of mice fed a control diet containing 20% ovalbumin (C). This reduced IgA PFC response was partiall...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::Experiments were designed to determine the in vivo cell-cycle phase of lymphocytes in secondary lymphoid follicles, and whether B-cell proliferation and apoptosis occur within follicular dendritic cell (FDC)-associated clusters. Using frozen serial sections of human tonsils, lymphoid follicles were stained to reveal h...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00203.x
更新日期:1997-04-01 00:00:00
abstract::Peripheral blood mononuclear (PBM) cells from five of 21 patients with common variable immunodeficiency (CVI) stimulated in vitro by antigen produced antibodies normally. In 11 of the 16 non-responders, removal of adherent suppressor cells by Sephadex G-10 or removal of monocytes or OKT8 positive suppressor lymphocyte...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::The gut microbiota provides an important stimulus for the induction of regulatory T (Treg) cells in mice, whether this applies to newborn children is unknown. In Swedish children, Staphylococcus aureus has become a common early colonizer of the gut. Here, we sought to study the effects of bacterial stimulation on neon...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12209
更新日期:2014-03-01 00:00:00
abstract::Oral tolerance promotes a generalized decrease in specific immune responsiveness to proteins previously encountered via the oral route. In addition, parenteral immunization with a tolerated protein also triggers a significant reduction in the primary responsiveness to a second unrelated antigen. This is generally expl...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02901.x
更新日期:2009-03-01 00:00:00
abstract::The role of the mouse homocytotropic antibodies in passive cutaneous anaphylaxis reaction was investigated. One class of antibody was heat stable, detected at 2 h but not at 48 h after passive transfer, and belonged to a sublcass of mouse IgG. The other was heat labile, detected at 2 h and 48 h after passive transfer,...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-04-01 00:00:00
abstract::Membrane attack complexes of complement occur around thyroid follicles in Graves' disease and Hashimoto's thyroiditis. The lytic potential of such complexes is controlled by membrane-bound and fluid phase regulators and we have investigated the role of these in autoimmune thyroid disease. By immunohistochemical staini...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Using the fluorescence-activated cell sorter (FACS), the percentages of T and B lymphocytes in the peripheral blood, spleen and lymph nodes of CBA syngeneic and allogeneic pregnancies were determined. At 19 days of syngeneic pregnancy, the level of T cells in the blood had decreased and that of B cells had increased. ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-04-01 00:00:00
abstract::Contact sensitization by epicutaneous application of picryl chloride causes in mice a significant reduction of the antibody responses to immunogenic TNP-conjugates. This split unresponsiveness is along-lasting. It was found that hapten applied on the skin became attached to the serum proteins and the transfer of such ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-08-01 00:00:00
abstract::Highly enriched bovine polymorphonuclear neutrophils (PMN) were able to destroy herpesvirus-infected cells in the presence of complement. Our results suggest that some viral antigens are capable of activating the alternate complement pathway directly which results in cytotoxicity however, the addition of PMN plus comp...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::Fas ligand (FasL) expression induces apoptosis of activated T cells and has been suggested as a strategy to inhibit graft rejection. Unfortunately, the use of FasL to confer 'immune privilege' in this setting has been hampered by the finding that it may also provoke a destructive granulocytic response. While the Fas/F...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02504.x
更新日期:2007-01-01 00:00:00
abstract::Specific and non-specific IgE antibody responses were studied in SD rats infected with between 5 and 2500 Nippostrongylus brasiliensis (NB) larvae. In rats with 2500 NB larvae, specific IgE antibody, measured by enzyme-linked immunosorbent assay (ELISA) using NB excretory/secretory substance as antigen, reached a peak...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Animals contain an enormous load of non-pathogenic bacteria in the lower intestine, which exploit an environment with a stable temperature and abundant carbon sources. Our load of bacteria outnumbers our own cells. In order to survive with such a high number of organisms in very close proximity to host tissues the int...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2005.02159.x
更新日期:2005-06-01 00:00:00
abstract::C57Bl/6 (B6) mice produced highly developed granulomata in the lung 3 or 4 weeks after intravenous injection with oil-associated BCG cell walls (CW), but C3H/He (C3H) mice did not. The potential to develop granulomata is genetically controlled (Yamamoto & Kakinuma, 1978). It is probable that the genes control only T-c...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::Specific IgE and IgG responses to highly purified Ambrosia (ragweed) allergens, Amb a V, Amb t V and Amb p V from the artemisiifolia (short), trifida (giant) and psilostachya (western) species are strongly associated with HLA-DR2 and Dw2 (DR2.2) in allergic Caucasoid individuals. To investigate the molecular basis of ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::The aim of this study is to investigate the clinical significance of the ratio between interleukin-17 (IL-17) secreting cell and FOXP3-positive regulatory T cell (FOXP3(+) Treg) infiltration in renal allograft tissues with acute T-cell-mediated rejection (ATCMR). Fifty-six patients with biopsy-proven ATCMR were includ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2012.03588.x
更新日期:2012-07-01 00:00:00
abstract::The cellular prion protein (PrPc) is a glycosyl-phosphatidylinositol (GPI)-linked cell surface protein, which is expressed at high density on nervous tissues and at lower levels on most other solid-organ tissues. It is also expressed on peripheral blood mononuclear cells (PBMC) of all lineages. In lymphocytes, its lev...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01166.x
更新日期:2001-02-01 00:00:00
abstract::It has been known for 15 years that the chicken epidermis contains ATPase+ and major histocompatibility complex class II-positive (MHCII+) dendritic cells. These cells were designated as Langerhans cells but neither their detailed phenotype nor their function was further investigated. In the present paper we demonstra...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02432.x
更新日期:2006-10-01 00:00:00