Co-expression of markers for hepatitis delta and hepatitis B viruses in human liver.

Abstract:

:Delta hepatitis (HDV) infection can only occur in the presence of hepatitis B (HBV) infection, as HDV requires a coat of HBV surface antigen (HBsAg) for assembly of complete virus. A number of studies have examined the variation of HBV markers in serum and liver during establishment of HDV infection, but none has systematically examined the relationship between the two viruses in individual hepatocytes. Liver biopsies from five patients with HDV/HBV infection were stained for HBsAg, HBV core antigen (HBcAg) and hepatitis D (delta) antigen (HDAg). Double immunostaining was performed with a combination of indirect immunoperoxidase and alkaline phosphatase/antialkaline phosphatase techniques. HDV and HBV antigens were expressed in all five liver biopsies. Co-localization of HBsAg was seen in up to 39% of HDAg positive cells, and HBcAg in up to 8% of HDAg positive cells. HBcAg was detectable in approximately 9% of HBsAg positive cells, and HBsAg in approximately 12% of HBcAg positive cells. HDV can replicate without HBV but ultimately requires HBV to produce complete virus and subsequently infect other cells. In this study the majority of HDV positive cells did not appear to contain HBV markers. This might suggest delta virus replication without assembly, or possibly sequential production/assembly of the virus.

journal_name

Histopathology

journal_title

Histopathology

authors

Ryley NG,Heryet AR,Goldin R,Monjardino J,Saldanha J,Fleming KA

doi

10.1111/j.1365-2559.1992.tb00990.x

keywords:

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

331-7

issue

4

eissn

0309-0167

issn

1365-2559

journal_volume

20

pub_type

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