Abstract:
:In cruciferous plants, the primary pre-rRNA cleavage site (P site) is immediately downstream of four similar, highly conserved sequences (A(1), A(2), A(3) and B) located within the 5'-ETS (5'-external transcribed spacer). In the present study, we describe the characterization of a plant NF D (nuclear factor D) that binds and interacts specifically with this A(123)BP cluster in the rDNA sequence. NF D is a high-molecular-mass complex containing nucleolin, fibrillarin and U3 and U14 snoRNAs. Furthermore, we show that NF D binds and cleaves pre-rRNA specifically at the P site. Thus we conclude that NF D is a pre-rRNA processing complex that may first assemble on rDNA and then bind nascent pre-rRNA.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Sáez-Vasquez J,Caparros-Ruiz D,Barneche F,Echeverría Mdoi
10.1042/BST0320578keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
578-80issue
Pt 4eissn
0300-5127issn
1470-8752pii
BST0320578journal_volume
32pub_type
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