Abstract:
:We attempted to characterize the cellular autofluorescence phenomenon of living HL-60 cells and to appraise its modifications under oxidative stress conditions induced by 1 alpha,25(OH)(2)D(3) (VD(3)) and its analog EB1089. Autofluorescence emission spectra of human promyelocytic HL-60 leukemic cells were monitored using laser scanning confocal microspectrofluorometry under UV excitation. Evaluation of reactive oxygen species (ROS) release was performed using the 2',7'-dichlorodihydrofluorescein diacetate (H(2)-DCFDA) staining and fluorescence emission measurement. VD(3) (1, 10, 100 nM) or EB1089 (0.1, 1 and 10 nM) induces a decrease in autofluorescence emission intensity that can be attributed to the oxidation of the coenzyme nicotinamide adenine dinucleotide (phosphate) NAD(P)H into NAD(P)(+). A dose-dependent increase (p<0.05) in ROS release is observed in VD(3)- and EB1089-treated cells. As compared with VD(3)- or EB1089-treated cells, doxorubicin-VD(3) or doxorubicin-EB1089 treatments strongly decrease the autofluorescence intensity and induce a higher release of ROS (p<0.05). The association of antioxidants (N-acetyl cysteine, superoxide dismutase, catalase) with VD(3) or EB1089 induce a more limited autofluorescence decrease and a weaker ROS generation, as compared with VD(3) and EB1089 treated cells. In conclusion, the free radicals release, generated by VD(3) and EB1089, was associated with the decrease in autofluorescence emission and can be modulated by doxorubicin and antioxidants.
journal_name
Technol Cancer Res Treatjournal_title
Technology in cancer research & treatmentauthors
Bondza-Kibangou P,Millot C,Dufer J,Millot JMdoi
10.1177/153303460400300409keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
383-91issue
4eissn
1533-0346issn
1533-0338pii
d=3018&c=4157&p=12415&do=detailjournal_volume
3pub_type
杂志文章abstract::We describe the first histopathologic analysis of prostatic tissue following hypofractionated robotic radiation therapy. A 66 year-old man presented with stage II, low risk adenocarcinoma of the prostate and underwent elective conformal hypofractionated radiation therapy. His pretreatment evaluation revealed T1c adeno...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303461000900606
更新日期:2010-12-01 00:00:00
abstract::Traditional imaging techniques, such as computed tomography (CT), have high spatial resolution for changes in the anatomy or structure of organs, but are not very useful for detecting early biochemical changes. Conversely, positron emission tomography (PET) relies on changes in molecular biology to identify the earlie...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.1177/153303460600500204
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abstract::The aim of this paper is to study the feasibility for determining the individual internal target volume (ITV) for hepatocellular carcinoma (HCC) using 3DCT associated with active breathing coordinator (ABC), comparing the ITVs defined by 3DCT associated with ABC and 4DCT. Thirteen patients with HCC after transarterial...
journal_title:Technology in cancer research & treatment
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doi:10.1177/153303461101000610
更新日期:2011-12-01 00:00:00
abstract:PURPOSE:The aim of the in vivo dosimetry, during the fractionated radiation therapy, is the verification of the correct dose delivery to patient. Nowadays, in vivo dosimetry procedures for photon beams are based on the use of the electronic portal imaging device and dedicated software to elaborate electronic portal ima...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818779201
更新日期:2018-01-01 00:00:00
abstract::Signal transduction pathways play a crucial role in breast cancer development, progression, and response to different therapies. A major problem in breast cancer therapy is the heterogeneity among different tumor types and cell lines commonly used in preclinical studies. To characterize the signaling pathways of some ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460600500601
更新日期:2006-12-01 00:00:00
abstract::Glucose transporter 1 (GLUT1) facilitates the cellular uptake of glucose and is overexpressed in most cancers. The altered expression of GLUT1 may influence the sensitivity of tumor cells to chemotherapy. This study investigated whether the knockdown of GLUT1 expression to sensitize head and neck cancer cells to the c...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500343
更新日期:2013-12-01 00:00:00
abstract::The study of the effectiveness of cryotherapy as a curative treatment for prostate cancer has often relied on the use of either in vitro cell culture monolayers or animal models. While the data gleaned from these studies have been valuable, each model has inherent limitations. In order to bridge the gap between in vit...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460700600204
更新日期:2007-04-01 00:00:00
abstract:OBJECTIVE:Acute myeloid leukemia (AML) represents a hematological cancer. The aim of the investigation was to probe the regulatory relevance of long non-coding RNA (lncRNA) aspartyl-tRNA synthetase anti-sense 1 (DARS-AS1)/microRNA-425 (miR-425)/transforming growth factor-beta 1 (TGFB1) to the development of AML. METHO...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820965580
更新日期:2020-01-01 00:00:00
abstract::Irreversible electroporation (IRE) has been shown to be an effective method of killing cells locally. In contrast to radiofrequency ablation, the mechanism by which cells are thought to die via IRE is the creation of pores in cell membranes, without substantial increase in tissue temperature. To determine the degree t...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrtexpress.2013.600253
更新日期:2014-12-01 00:00:00
abstract::The present approach at our institution for the treatment of patients with colorectal (CRC) cancer and with liver metastases planned for metastasectomy is the neoadjuvant administration of Bevacizumab with Irinotecan based therapy. Metabolic imaging of tumor viability with 18F-fluorodeoxyglucose (FDG) positron emissio...
journal_title:Technology in cancer research & treatment
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更新日期:2006-02-01 00:00:00
abstract::Although an MRI scanner is a single stand-alone modality, different acquisition techniques may be applied to collect structural and functional information, including vascular or angiogenic properties measured by dynamic contrast enhanced MRI (DCE-MRI) and Choline (Cho) metabolism measured by proton MR spectroscopy ima...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to investigate the prognostic role of neutrophil to lymphocyte ratio in ovarian cancer. Growing number of articles reported the relationship between neutrophil to lymphocyte ratio and prognosis in ovarian cancer, but the results remains inconclusive. The meta-analysis was conducted to...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,meta分析
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更新日期:2018-01-01 00:00:00
abstract::Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) is able to detect breast cancer with high sensitivity. Furthermore, this method provides functional information on tissue composition and vascularization. This study aims to identify the potential of DCE-MRI to predict distant metastasis in breast cancer p...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500266
更新日期:2012-12-01 00:00:00
abstract:PURPOSE:To develop and evaluate an automatic intensity-modulated radiation therapy (IMRT) program for cervical cancer, including a Convolution Neural Network (CNN)-based prediction model and an automated optimization strategy. METHODS:A CNN deep learning model was trained to predict a patient-specify set of IMRT objec...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820957002
更新日期:2020-01-01 00:00:00
abstract::This article proposes quantitative analysis tools and digital phantoms to quantify intrinsic errors of deformable image registration (DIR) systems and establish quality assurance (QA) procedures for clinical use of DIR systems utilizing local and global error analysis methods with clinically realistic digital image ph...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034614553891
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:We compared radiotherapy plans among helical tomotherapy (HT), volumetric-modulated arc therapy (VMAT), and intensity-modulated proton therapy (IMPT) for angiosarcoma of the scalp (AS). METHODS:We conducted a planning study for 19 patients with AS. The clinical target volume (CTV) 1 and CTV2 were defined as ...
journal_title:Technology in cancer research & treatment
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doi:10.1177/1533033820985866
更新日期:2021-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033819876263
更新日期:2019-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820970665
更新日期:2020-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615626011
更新日期:2017-02-01 00:00:00
abstract::In order to identify the proteomic differences between renal cell carcinoma (RCC) and benign renal tumors, we analyzed 168 serum samples from 65 RCC patients, 34 patients with benign renal tumors, and 69 healthy persons using the IMAC-Cu2+ ProteinChip system by surface enhanced laser desorption/ionization mass spectro...
journal_title:Technology in cancer research & treatment
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doi:10.1177/153303460900800307
更新日期:2009-06-01 00:00:00
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500437
更新日期:2015-12-01 00:00:00
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journal_title:Technology in cancer research & treatment
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033819849067
更新日期:2019-01-01 00:00:00
abstract::Non-thermal irreversible electroporation (N-TIRE) has shown promise as an ablative therapy for a variety of soft-tissue neoplasms. Here we describe the therapeutic planning aspects and first clinical application of N-TIRE for the treatment of an inoperable, spontaneous malignant intracranial glioma in a canine patient...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500181
更新日期:2011-02-01 00:00:00
abstract:PURPOSE:To evaluate 2 simultaneous integrated boost treatment planning techniques using helical tomotherapy for breast conserving therapy with regard to acute skin toxicity and dosimetry. METHODS:Thirty-two patients were studied. The original approach was for 16 patients and incorporated a directional block of the ips...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615574387
更新日期:2016-04-01 00:00:00
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820945798
更新日期:2020-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
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更新日期:2020-01-01 00:00:00
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journal_title:Technology in cancer research & treatment
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更新日期:2016-06-01 00:00:00
abstract::A review of the literature on the current applications of breast magnetic resonance imaging (MRI) indications, their rationale and their place in diagnosis and management of breast cancer was given. Contrast-enhanced breast MRI is developing as a valuable adjunct to mammography and sonography. Its high sensitivity for...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.1177/153303460400300602
更新日期:2004-12-01 00:00:00