Abstract:
OBJECTIVE:Acute myeloid leukemia (AML) represents a hematological cancer. The aim of the investigation was to probe the regulatory relevance of long non-coding RNA (lncRNA) aspartyl-tRNA synthetase anti-sense 1 (DARS-AS1)/microRNA-425 (miR-425)/transforming growth factor-beta 1 (TGFB1) to the development of AML. METHODS:The DARS-AS1 expression in bone marrow tissues was first analyzed in healthy subjects and AML patients. Subsequently, AML cell lines with DARS-AS1 knockdown were constructed, followed by cell proliferation and apoptosis assays. Afterward, downstream miRNA of DARS-AS1 and target mRNA of the miRNA were analyzed by bioinformatics, and their binding relationships were verified. Functional rescue experiments were then implemented. Finally, activation of the Smad2/3 signaling in MV4-11 and BF-24 cells were detected by western blot. RESULTS:DARS-AS1 was overexpressed in bone marrow tissues of AML patients and cells, and DARS-AS1 knockdown suppressed the proliferation of AML cells and induced apoptosis. DARS-AS1 bound to and negatively correlated with miR-425. Further results suggested that TGFB1 might be a target gene of miR-425 and could promote Smad2/3 phosphorylation and nuclear translocation. Finally, DARS-AS1 depletion could diminish the tumor volume in vivo. CONCLUSION:All in all, we highlighted here that DARS-AS1 enhanced the expression of TGFB1 through binding to miR-425 to modulate AML progression via the Smad2/3 pathway, which might perform as a therapeutic target for AML.
journal_name
Technol Cancer Res Treatjournal_title
Technology in cancer research & treatmentauthors
Dou B,Jiang Z,Chen X,Wang C,Wu J,An J,Sheng Gdoi
10.1177/1533033820965580subject
Has Abstractpub_date
2020-01-01 00:00:00pages
1533033820965580eissn
1533-0346issn
1533-0338journal_volume
19pub_type
杂志文章abstract::This study aimed to analysis outcome and prognosis of interstitial 125I seed implantation in patients with cervical lymph node recurrence after multimodal treatment of thoracic esophageal squamous cell carcinoma (ESCC). We conducted a retrospective review of 19 patients with 32 cervical lymph nodes recurrences after m...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500409
更新日期:2015-04-01 00:00:00
abstract:PURPOSE:The efficacy of image-guided high-dose rate brachytherapy for cervical cancer is limited by the ineffective rectal sparing devices available commercially and the potential applicator movement. We developed a novel device using a balloon catheter and a belt immobilization system, serving for rectal dose reductio...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034616631122
更新日期:2017-06-01 00:00:00
abstract::The combined effect of high energy shock waves (HESW), generated by a piezoelectric device, and paclitaxel on Mat B-III rat breast cancer cells in vitro and in an in vivo animal model is presented. A significant reduction of in vitro Mat B-III cell proliferation versus cells treated with paclitaxel alone was observed ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460800700204
更新日期:2008-04-01 00:00:00
abstract::In cryosurgery it is crucial that the performance of cryoprobes is predictable and constant. In this study we tested the intra- and interneedle variation between 17-gauge cryoprobes in two homogeneous mediums. Also, a multiprobe setup was tested. Cryoprobe performance was defined as the time it takes one cryoprobe to ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460800700406
更新日期:2008-08-01 00:00:00
abstract::This study quantifies the setup uncertainties to optimize the planning target volume (PTV) margin based on daily image guidance, its dosimetric impact, and radiobiological implication for intensity-modulated radiation therapy (IMRT) in head and neck cancer. Ten patients were retrospectively chosen who had been treated...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034614561162
更新日期:2016-02-01 00:00:00
abstract::We describe the first histopathologic analysis of prostatic tissue following hypofractionated robotic radiation therapy. A 66 year-old man presented with stage II, low risk adenocarcinoma of the prostate and underwent elective conformal hypofractionated radiation therapy. His pretreatment evaluation revealed T1c adeno...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303461000900606
更新日期:2010-12-01 00:00:00
abstract:UNLABELLED:The purpose of this study was to compare the prognostic value of the percentage of positive biopsy cores (PPBC), the percentage of cancer volume (PCV), and the maximum involvement of biopsy cores (MIBC) as a prognostic factor in low- and intermediate-risk patients with clinically localized prostate cancer wh...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrtexpress.2013.600271
更新日期:2014-06-01 00:00:00
abstract::Brachytherapy has the advantage of delivering a high dose to the tumor while sparing the surrounding normal tissues. With proper case selection and delivery technique, high-dose-rate (HDR) brachytherapy has great promise, because it eliminates radiation exposure, allows short treatment times, and can be performed on a...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.1177/153303460400300305
更新日期:2004-06-01 00:00:00
abstract:PURPOSE:We investigated the dosimetric effect of intrafraction tumor motion in lung stereotactic body radiotherapy using the CyberKnife static tracking system. METHODS:Four-dimensional computed tomography scans of a dynamic thorax phantom were acquired. Two motion ranges, 3 collimator sizes, and 4 treatment starting p...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033819859448
更新日期:2019-01-01 00:00:00
abstract::At external beam radiotherapy, stereoscopic X-ray imaging system is responsible as tumor motion information provider. This system takes X-ray images intermittently from tumor position (1) at pretreatment step to provide training data set for model construction and (2) during treatment to control the accuracy of correl...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034616638803
更新日期:2017-04-01 00:00:00
abstract:OBJECTIVE:We compared radiotherapy plans among helical tomotherapy (HT), volumetric-modulated arc therapy (VMAT), and intensity-modulated proton therapy (IMPT) for angiosarcoma of the scalp (AS). METHODS:We conducted a planning study for 19 patients with AS. The clinical target volume (CTV) 1 and CTV2 were defined as ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820985866
更新日期:2021-01-01 00:00:00
abstract::The microRNA-200 (miR-200) family has been reported to be vital for the inhibition of epithelial-to-mesenchymal transition (EMT) in tumor cells. The miR-200 family represents a complex multi-factorial regulatory network which has not been well described in breast cancer. This study aimed to clarify the underlying regu...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820979672
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Palliation of advanced disease using radiotherapy can create difficult clinical situations where standard computed tomography simulation and immobilization techniques are not feasible. We developed a linear accelerator-based radiotherapy simulation technique using nonstandard patient positioning for head and...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033819865623
更新日期:2019-01-01 00:00:00
abstract::The 3-D transrectal ultrasound (TRUS)-guided prostate biopsy system is a novel device that allows precise needle placement in a template fashion. We evaluate its utility for prostate cancer (PCa) detection. A retrospective analysis was performed evaluating 68 prospective patients at the Duke Prostate Center who underw...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460900800202
更新日期:2009-04-01 00:00:00
abstract::Endometrial carcinoma is one of the most common cancers in women. A limited number of endometrial carcinoma cell lines are available for studies of signal transduction pathways and experimental therapeutics in vitro. However, these cell lines have not been comprehensively characterized. In this study, we used genome-w...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303461000900207
更新日期:2010-04-01 00:00:00
abstract::We used a shifted-excitation Raman difference spectroscopy method for the ex vivo classification of resected and formalin-fixed breast tissue samples as normal (healthy) tissue, fibroadenoma, or invasive carcinoma. We analyzed 8 tissue samples containing invasive carcinoma that were surrounded by normal tissue and 3 t...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818782532
更新日期:2018-01-01 00:00:00
abstract::The purpose of this study was to evaluate the feasibility and treatment plans of intensity-modulated radiation therapy using helical tomotherapy (HT) for brain metastases. Twenty-three patients with 1 to 4 brain metastases were treated with HT. In combination with whole-brain radiotherapy (simultaneous plans), metasta...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460800700602
更新日期:2008-12-01 00:00:00
abstract::Despite remarkable progress in diagnosis and treatment, malignant glioma, a highly lethal cancer of the central nervous system, remains incurable. Although glioma stem-like cells (GSCs) represent a relatively small fraction of the cells in malignant glioma, they can proliferate and self renew extensively, being crucia...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500277
更新日期:2012-12-01 00:00:00
abstract::The study of the effectiveness of cryotherapy as a curative treatment for prostate cancer has often relied on the use of either in vitro cell culture monolayers or animal models. While the data gleaned from these studies have been valuable, each model has inherent limitations. In order to bridge the gap between in vit...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460700600204
更新日期:2007-04-01 00:00:00
abstract::Most experimental studies of electroporation focus on permeabilization of the outer cell membrane. Some experiments address delivery of ions and molecules into cells that should survive; others focus on efficient killing of the cells with minimal temperature rise. A basic method for quantifying electroporation effecti...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818792491
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:We aimed to identify the expression of Sal-like 4 (SALL4) in breast cancer tissues and to explore the role of this gene in the carcinogenesis of breast cancer cells. METHODS:A total of 62 paired breast cancer and noncancerous tissue samples were obtained from patients with breast cancer. SALL4 expression pat...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820980074
更新日期:2020-01-01 00:00:00
abstract::Blood flow is a key parameter in studying cancer angiogenesis and hypermetabolism. Current photoacoustic blood flow estimation methods focus on either the axial or transverse component of the flow vector. However, the Doppler angle (beam-to-flow angle) is needed to calculate the total flow speed, and it cannot always ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500278
更新日期:2012-08-01 00:00:00
abstract::The purpose of this work is to estimate the degree of uncertainty inherent to a given four dimensional computed tomography (4D-CT) imaging modality and to test for interaction of the investigated factors (i.e., object displacement, velocity, and the period of motion) when determining the object motion coordinates, mot...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303461000900309
更新日期:2010-06-01 00:00:00
abstract:AIM:To explore the relationship of clinicopathological features and the proteins of C-met expression in the prognosis of cholangiocarcinoma. METHODS:Clinical data and the completed follow-up information of patients with cholangiocarcinoma who underwent cholangiocarcinoma operation from January 2004 to December 2010 we...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615578959
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:Circulating tumor cells (CTCs) hold huge potential for both clinical applications and basic research into the management of cancer, but the relationship between CTC count and cervical cancer prognosis remains unclear. Therefore, research on this topic is urgently required. OBJECTIVE:This study investigated ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820957005
更新日期:2020-01-01 00:00:00
abstract::Optical methods such as reflectance and fluorescence spectroscopy are being investigated for their potential to aid cancer detection in a quantitative, minimally invasive manner. Mathematical models of reflectance and fluorescence provide an important link between measured optical data and biomedically-relevant tissue...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.7785/tcrt.2012.500187
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the mechanisms involved with miRNA-708 and its targeting of bone morphogenetic protein and activin membrane-bound inhibitor in cell proliferation, migration, and apoptosis in mice with melanoma via the Wnt and transforming growth factor β signaling pathways. METHODS:Sixt...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034618756784
更新日期:2018-01-01 00:00:00
abstract::Targeted immunotherapy using dendritic cell vaccine has been employed for the treatment of solid tumors. Topical 5-aminolevulinic acid-mediated photodynamic therapy, an established approach for topical cancers, can induce an effective antitumor immune response. We have previously shown that 5-aminolevulinic acid-media...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818785275
更新日期:2018-01-01 00:00:00
abstract::Complement 3a (C3a) and complement 5a (C5a), small cleavage fragments generated by complement activation, has been previously shown to be obviously up-regulated in highly metastatic hepatocellular carcinoma (HCC) cells. However, their functional roles in HCC cells remains unclear. Here, we investigated the biological ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820970668
更新日期:2020-01-01 00:00:00
abstract::Fascin is overexpressed in various tumor tissues and is closely related to tumor metastasis and invasion. However, the role of fascin in cholangiocarcinoma RBE cells has not been clearly reported. This study aimed to establish a cholangiocarcinoma cell line with stable and high expression of fascin to observe the effe...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615580696
更新日期:2016-04-01 00:00:00