Abstract：
OBJECTIVE:To determine the response of immature articular cartilage to both sharp and blunt trauma in terms of cell death, cell proliferation and matrix synthesis. DESIGN:Blunt wounds were made with a trephine in full depth immature bovine articular cartilage explants which were cut in half through the center of the trephine wound with a sharp scalpel to produce blunt and sharp trauma on the same explant. Explants were maintained in culture for up to 10 days. Prior to fixation at days 2, 5 and 10, medium was supplemented with 10 microCi ml-1 35S-sulphate, [3H]-proline or [3H]-thymidine for 24h to assess matrix synthesis and cell proliferation. Cell death was assessed using a Live/Dead label. RESULTS:In the case of blunt wounds, a band of cell death was observed adjacent to the lesion edge. Microautoradiography demonstrated little radiolabel incorporation and, therefore, no new matrix synthesis or cell proliferation within this region. In contrast, wounds made with a sharp scalpel showed restricted cell death, with radiolabel incorporation adjacent to the lesion edge at all time points. This demonstrated not only chondrocyte proliferation and new matrix synthesis at the wound margin, but also an up-regulation of matrix synthesis adjacent to the lesion edge. CONCLUSIONS:In terms of clinical relevance, the use of sharp precise instruments during the surgical management of cartilage defects may be necessary to reduce cell death and promote matrix elaboration at the lesion edge in order to facilitate successful integration.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Redman SN,Dowthwaite GP,Thomson BM,Archer CWdoi
10.1016/j.joca.2002.12.001keywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
106-16issue
2eissn
1063-4584issn
1522-9653pii
S1063458403002759journal_volume
12pub_type
杂志文章abstract：OBJECTIVE:To determine the interrelations between degenerative changes in articular cartilage and underlying trabecular bone during development of osteoarthritis and to test the ability of quantitative magnetic resonance imaging (MRI) to detect those changes. METHODS:Human cadaver patellae were investigated with quant...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.03.019
更新日期：2007-10-01 00:00:00
abstract：OBJECTIVE:A recent genome-wide association study reported significant associations of genetic variants within the ALDH1A2 gene with osteoarthritis (OA) of the hand in European populations. However, these findings have not been well generalized to other joints, or to other populations. METHODS:We performed a two-stage ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2017.01.003
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:The purpose of the current study was to investigate the feasibility of applying defect-size femoral implants for the treatment of localized cartilage defects in a 1-year follow-up model. METHODS:In 13 goats, a medial femoral condyle defect was created in both knees. Defects were randomly treated by immediate...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2009.10.009
更新日期：2010-03-01 00:00:00
abstract：OBJECTIVE:Test the hypothesis that greater baseline peak external knee adduction moment (KAM), KAM impulse, and peak external knee flexion moment (KFM) during the stance phase of gait are associated with baseline-to-2-year medial tibiofemoral cartilage damage and bone marrow lesion progression, and cartilage thickness ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.02.005
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVE:To provide an insightful summary of studies on biochemical markers for osteoarthritis (OA). DESIGN:Two investigators systematically searched the electronic PubMed database for clinical studies into soluble biochemical markers for OA in humans that were published between 01-03-2018 and 01-03-2019. Data from s...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2019.11.007
更新日期：2020-03-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the site-dependent changes in the structure and function of articular cartilage in the lapine knee joint at a very early stage of osteoarthritis (OA), created experimentally by anterior cruciate ligament transection (ACLT). METHODS:Unilateral ACLT was performed in eig...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.04.010
更新日期：2014-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy and safety of chondroitin sulfate and/or glucosamine hydrochloride in alleviating symptoms and improving the dysfunction of Kashin-Beck disease (KBD) patients. METHODS:We undertook a cluster-randomized, placebo-controlled trial in 251 patients with KBD. Participants were randomly all...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joca.2012.03.013
更新日期：2012-07-01 00:00:00
abstract：:The efficacy and the safety of intra-articular injections of sodium hyaluronate were studied in patients with osteoarthritis of the knee in a randomized multicenter double-blind study. Two hundred and nine patients received five injections of either 25 mg hyaluronate/2.5 ml (verum, N = 102) or 0.25 mg hyaluronate/2.5 ...
journal_title：Osteoarthritis and cartilage
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s1063-4584(05)80329-2
更新日期：1993-10-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate the morphological changes of the lateral meniscus in end-stage lateral compartment osteoarthritis (OA) of the knee. METHODS:One hundred fifty-eight knee joints from 133 patients that subsequently underwent total knee joint arthroplasty from January 2008 to December 2009 ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2011.11.005
更新日期：2012-02-01 00:00:00
abstract：OBJECTIVE:We investigated whether innate differences in cytokine response were associated with the absence of osteoarthritis (OA) in old age. DESIGN:In 82 participants from a cross-sectional birth cohort, radiographs of hands, hips and knees were taken at the age of 90 years. OA was defined as a Kellgren-Lawrence scor...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2010.03.016
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVE:Physical activity (PA) in the US knee osteoarthritis (OA) population is low, despite well-established health benefits. PA program implementation is often stymied by sustainability concerns. We sought to establish parameters that would make a short-term (3-year efficacy) PA program a cost-effective component o...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2020.01.017
更新日期：2020-06-01 00:00:00
abstract：:The extracellular matrix of articular cartilage consists mainly of type II collagen and large aggregating proteoglycan (aggrecan). During arthritis and other joint diseases, the proteoglycan (PG) level of cartilage matrix is diminished, leading to impairment of normal joint function. A new method is described for meas...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(05)80070-6
更新日期：1994-09-01 00:00:00
abstract：OBJECTIVE:The objectives for this study were to determine whether radical initiated photopolymerizations typically employed for cell encapsulations lead to oxidative stress incurred by chondrocytes and whether the development of a pericellular matrix (PCM) decreases this oxidative stress and has longer-term benefits on...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2012.06.015
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVE:Moderate mechanical stress generated by normal joint loading and movement is essential for the maintenance of healthy articular cartilage. However, the effects of reduced loading caused by the absence of weight bearing or joint motion on articular cartilage and subchondral bone is still poorly understood. We ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.11.013
更新日期：2017-05-01 00:00:00
abstract：:This study sought to assess the relative levels of the mRNAs of the core proteins of the small proteoglycans (PGs) biglycan, decorin and fibromodulin in the hypertrophic phase of the early osteoarthritis (OA) that follows joint injury. Experimental OA was induced in eight dogs by transection of the anterior cruciate l...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(96)80015-x
更新日期：1996-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy of preparations with avocado-soybean unsaponifiables (ASUs) in osteoarthritis (OA) patients using meta-analysis on randomized controlled trials (RCTs). METHOD:RCTs from systematic searches were included if they explicitly stated that hip and/or knee OA patients were randomized to eit...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.joca.2007.10.003
更新日期：2008-04-01 00:00:00
abstract：OBJECTIVE:The prevalence of osteoarthritis (OA) varies between joints. Cartilage in eight different joints was evaluated to elucidate the disparate susceptibilities between joints to post-traumatic OA (PTOA) and provide evidence for joint-specific clinical treatments. The hypothesis was that cartilage in different join...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.02.023
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVE:To determine the effects of interleukin (IL)-6 and oncostatin M (OSM) added separately or in combination with IL-1beta on human osteoarthritic (OA) chondrocytes in alginate beads. DESIGN:Human chondrocytes were isolated from OA cartilage and cultured in alginate beads for 12 days, in the absence or in the pr...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2004.06.011
更新日期：2004-10-01 00:00:00
abstract：OBJECTIVE:To validate an automatic scheme for the segmentation and quantitative analysis of the medial meniscus (MM) and lateral meniscus (LM) in magnetic resonance (MR) images of the knee. METHOD:We analysed sagittal water-excited double-echo steady-state MR images of the knee from a subset of the Osteoarthritis Init...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.06.029
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:Macromolecules of the articular cartilage extracellular matrix released into synovial fluid, blood, or urine can serve as potentially useful biomarkers of the severity of osteoarthritis (OA). Biomechanical factors play an important role in OA pathogenesis, yet their influence on biomarker production is not we...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2005.07.003
更新日期：2005-12-01 00:00:00
abstract：OBJECTIVE:A significant risk factor for anterior cruciate ligament (ACL) tears in young athletes is a reduced femoral Notch Width Index (NWI). The purpose of this study was to test if persons with knee osteoarthritis (OA) and ACL tears have smaller NWI independent of prior joint injury and osteophyte volume. METHODS:W...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,多中心研究
doi：10.1016/j.joca.2009.09.006
更新日期：2010-02-01 00:00:00
abstract：OBJECTIVE:To describe the reproducibility and validity of six different measurement techniques for knee subchondral bone mineral density (sBMD). METHODS:A consecutive sample of 50 male and female participants from a population-based longitudinal study had sBMD assessed using dual energy X-ray absorptiometry scans. Ant...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2008.04.012
更新日期：2008-12-01 00:00:00
abstract：OBJECTIVES:Based on the concept of a systemic predisposition for articular cartilage calcification (CC), the aim of this study was to determine the prevalence and amount of bilateral CC of hip and knee joints in an unselected sample cohort by high-resolution digital contact radiography (DCR) and to analyze the associat...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.06.020
更新日期：2016-12-01 00:00:00
abstract：OBJECTIVE:To develop flow cytometry for the study of human articular cartilage cell phenotype and to validate the method on chondrocytes cultured in different in-vitro systems. METHODS:Chondrocyte phenotype was modulated by culturing the cells under different in-vitro conditions: i.e. in monolayer and in suspension cu...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2000.0352
更新日期：2001-01-01 00:00:00
abstract：OBJECTIVE:We aimed to (1) determine reference values for trochlear morphology and patellofemoral (PF) alignment in adults without magnetic resonance imaging (MRI)-defined PF full thickness cartilage damage or knee pain; and (2) evaluate dose-response patterns for these measures with prevalent MRI-defined PF structural ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2017.06.005
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:To investigate individual preferences for physical activity (PA) attributes in adults with chronic knee pain, to identify clusters of individuals with similar preferences, and to identify whether individuals in these clusters differ by their demographic and health characteristics. DESIGN:An adaptive conjoint...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,多中心研究
doi：10.1016/j.joca.2018.10.002
更新日期：2019-02-01 00:00:00
abstract：OBJECTIVES:Determine the depth-varying confined and osmotic compression moduli of normal human articular cartilage from the femoral head, and test whether these moduli are dependent on fixed charge density. METHODS AND RESULTS:Using an automated instrument to allow epifluorescence microscopy analysis during confined c...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2001.0424
更新日期：2001-08-01 00:00:00
abstract：:Osteoarthritis is characterized by progressive breakdown of articular cartilage. This review summarizes findings of the last year, which shed new light on mechanisms and factors involved in cartilage loss. Evidence is accumulating that the transcription factor hypoxia-inducible factor-2α (HIF-2α) is highly enhanced in...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2011.01.022
更新日期：2011-04-01 00:00:00
abstract：OBJECTIVE:Cam hip morphology is associated with femoroacetabular impingement (FAI) syndrome and causes hip osteoarthritis (OA). We aimed to assess the prevalence of cam hip morphology in a sample representative of the general population, using a measure with a predefined diagnostic accuracy. DESIGN:Patients aged 16-65...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2018.09.019
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:Osteophytes are non-neoplastic osteo-cartilaginous protrusions growing at the margins of osteoarthritic joints. They can not only be considered as in situ repair tissue, but also represent an excellent in vivo model for induced cartilage repair processes. Our focus was to identify different steps of osteophyt...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2002.0873
更新日期：2003-02-01 00:00:00
