Expression of biglycan, decorin and fibromodulin in the hypertrophic phase of experimental osteoarthritis.


:This study sought to assess the relative levels of the mRNAs of the core proteins of the small proteoglycans (PGs) biglycan, decorin and fibromodulin in the hypertrophic phase of the early osteoarthritis (OA) that follows joint injury. Experimental OA was induced in eight dogs by transection of the anterior cruciate ligament. Articular cartilage was harvested from each joint, the total RNA was extracted and the concentration of DNA in the cartilage was measured. The relative levels of mRNA for biglycan, decorin and fibromodulin were assessed by northern blot analyses. An increase in cartilage mass with no increase in DNA concentration confirmed that the joints were in the hypertrophic phase that follows joint injury. The total RNA per microgram of DNA was increased 2.5 times. Compared with control cartilage, the mRNA levels in osteoarthritic cartilage, when normalized to the concentration of DNA, were increased 3.9 times for biglycan, 1.2 times for decorin and 2.4 times for fibromodulin. Because these small PGs affect collagen fibrillogenesis in vitro, their discoordinate metabolism may contribute to the abnormal collagen formation and deposition that occurs in OA and to the ultimate failure of the articular cartilage.


Dourado GS,Adams ME,Matyas JR,Huang D




Has Abstract


1996-09-01 00:00:00














  • Pain, motor and gait assessment of murine osteoarthritis in a cruciate ligament transection model.

    abstract:OBJECTIVE:The major complaint of Osteoarthritis (OA) patients is pain. However, due to the nature of clinical studies and the limitation of animal studies, few studies have linked function impairment and behavioral changes in OA animal models to cartilage loss and histopathology. Our objective was to study surrogate ma...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Ruan MZ,Patel RM,Dawson BC,Jiang MM,Lee BH

    更新日期:2013-09-01 00:00:00

  • The groove model of osteoarthritis applied to the ovine fetlock joint.

    abstract:OBJECTIVE:Until now there have been no appropriate models for metacarpophalangeal osteoarthritis (OA), even though OA in this joint is a significant medical and economic problem in horses. A good model would be useful to evaluate progression and treatment of OA, particularly in this joint. Therefore, we translated the ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Mastbergen SC,Pollmeier M,Fischer L,Vianen ME,Lafeber FP

    更新日期:2008-08-01 00:00:00

  • Occupational risk factors for osteoarthritis of the knee: a meta-analysis.

    abstract:INTRODUCTION:Systematic reviews agree that knee osteoarthritis (OA) is related to occupational activities, but have not quantified the overall risks. METHODS:Systematic review of observational studies of knee OA and occupation. Job titles, elite sport, heavy work, kneeling, and other activities were included. Relative...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,meta分析,评审


    authors: McWilliams DF,Leeb BF,Muthuri SG,Doherty M,Zhang W

    更新日期:2011-07-01 00:00:00

  • T-cell mediated inflammatory pathway in osteoarthritis.

    abstract::Osteoarthritis (OA) is not caused by a simple consequence of aging and cartilage degradation. Based on the conventional paradigm, OA has been considered a degenerative joint disorder. However, the dominant clinical symptom has been characterized by a non-infectious chronic inflammatory condition with infiltration of i...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Nakamura H,Yoshino S,Kato T,Tsuruha J,Nishioka K

    更新日期:1999-07-01 00:00:00

  • Characterization of proteoglycan production and processing by chondrocytes and BMSCs in tissue engineered constructs.

    abstract:OBJECTIVE:The goal of this study was to characterize the proteoglycan (PG) production and processing by bone marrow stromal cells (BMSCs) within a tissue engineered construct. METHODS:Bovine BMSCs and articular chondrocytes (ACs) were isolated from an immature calf, seeded into agarose gels, and cultured up to 32 days...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Connelly JT,Wilson CG,Levenston ME

    更新日期:2008-09-01 00:00:00

  • Measurement properties of the WOMAC LK 3.1 pain scale.

    abstract:OBJECTIVE:The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) is applied extensively to patients with osteoarthritis of the hip or knee. Previous work has challenged the validity of its physical function scale however an extensive evaluation of its pain scale has not been reported. Our purpose wa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Stratford PW,Kennedy DM,Woodhouse LJ,Spadoni GF

    更新日期:2007-03-01 00:00:00

  • Skin and urine pentosidine weakly correlate with joint damage in a cohort of patients with early signs of osteoarthritis (CHECK).

    abstract:OBJECTIVES:Age-related changes in articular cartilage are likely to play a role in the aetiology of osteoarthritis (OA). One of the major age-related changes in cartilage is the accumulation of advanced-glycation-endproducts (AGEs). Since, cartilage tissue is not readily available from patients for studying AGE levels,...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究


    authors: Vos PA,DeGroot J,Huisman AM,Oostveen JC,Marijnissen AC,Bijlsma JW,van El B,Zuurmond AM,Lafeber FP

    更新日期:2010-10-01 00:00:00

  • Prevalence of cam and pincer-type deformities on hip MRI in an asymptomatic young Swiss female population: a cross-sectional study.

    abstract:OBJECTIVES:Femoroacetabular impingement is proposed to cause early osteoarthritis (OA) in the non-dysplastic hip. We previously reported on the prevalence of femoral deformities in a young asymptomatic male population. The aim of this study was to determine the prevalence of both femoral and acetabular types of impinge...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Leunig M,Jüni P,Werlen S,Limacher A,Nüesch E,Pfirrmann CW,Trelle S,Odermatt A,Hofstetter W,Ganz R,Reichenbach S

    更新日期:2013-04-01 00:00:00

  • In vivo patellofemoral contact mechanics during active extension using a novel dynamic MRI-based methodology.

    abstract:OBJECTIVES:To establish an in vivo, normative patellofemoral (PF) cartilage contact mechanics database acquired during voluntary muscle control using a novel, dynamic, magnetic resonance (MR) imaging-based, computational methodology and validate the contact mechanics sensitivity to the known sub-millimeter methodologic...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Borotikar BS,Sheehan FT

    更新日期:2013-12-01 00:00:00

  • The association between patella alignment and knee pain and function: an MRI study in persons with symptomatic knee osteoarthritis.

    abstract:OBJECTIVE:The aim of the study was to examine the association between patellofemoral (PF) alignment (using standard magnetic resonance imaging (MRI) images of extended knees) and knee pain and function. DESIGN:Subjects were recruited to participate in a natural history study of symptomatic knee osteoarthritis, called ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kalichman L,Zhu Y,Zhang Y,Niu J,Gale D,Felson DT,Hunter D

    更新日期:2007-11-01 00:00:00

  • Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice.

    abstract:OBJECTIVE:To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice. METHODS:Col2a1-CreER(T2) transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER(T2);R26R mice were administered TM for 5 day...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Zhu M,Chen M,Lichtler AC,O'Keefe RJ,Chen D

    更新日期:2008-01-01 00:00:00

  • Adenoviral overexpression of Smad-7 and Smad-6 differentially regulates TGF-beta-mediated chondrocyte proliferation and proteoglycan synthesis.

    abstract:OBJECTIVE:To assess if various biological responses to transforming growth factor-beta (TGF-beta) in chondrocytes are differentially regulated by Smad-6 and Smad-7. DESIGN:Adenoviral overexpression of Smad-6 or -7 mRNA in a chondrocyte cell line was determined via semi-quantitative RT-PCR and protein overexpression wa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Scharstuhl A,Diepens R,Lensen J,Vitters E,van Beuningen H,van der Kraan P,van den Berg W

    更新日期:2003-11-01 00:00:00

  • Sensitivity to change of the functional index for hand osteoarthritis.

    abstract:BACKGROUND:The functional index for hand osteoarthritis (FIHOA), a 10-item investigator-administered questionnaire, was validated in 1995. It is relevant, reliable and has good external and internal validities. OBJECTIVE:To assess the sensitivity to change over time of the FIHOA. PATIENTS/METHODS: PATIENTS:symptomat...

    journal_title:Osteoarthritis and cartilage

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Dreiser RL,Maheu E,Guillou GB

    更新日期:2000-01-01 00:00:00

  • Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients.

    abstract:OBJECTIVE:Synovitis associated with osteoarthritis (OA) is directly responsible for several clinical symptoms and reflects OA's structural progression. This study sought to analyze the expression of proinflammatory mediations, including Interleukin (IL)-17 and IL-22, which play key roles in regulating inflammatory proc...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Deligne C,Casulli S,Pigenet A,Bougault C,Campillo-Gimenez L,Nourissat G,Berenbaum F,Elbim C,Houard X

    更新日期:2015-11-01 00:00:00

  • Osteoarthritis year 2012 in review: imaging.

    abstract:PURPOSE:This article reviews original publications related to imaging in osteoarthritis (OA) published in English from September 2011 through March 2012. In vitro data and animal studies are not covered. METHODS:To extract relevant studies, an extensive PubMed database search was performed using the query terms "osteo...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Roemer FW,Guermazi A

    更新日期:2012-12-01 00:00:00

  • The efficacy, safety and carry-over effect of diacerein in the treatment of painful knee osteoarthritis: a randomised, double-blind, NSAID-controlled study.

    abstract:OBJECTIVE:To evaluate the efficacy, safety and carry-over effect of diacerein, in comparison to piroxicam, in the treatment of Thai patients with symptomatic knee osteoarthritis (OA). DESIGN:This was a double-blind, randomised, piroxicam-controlled, parallel-group study. A 7-day non-steroidal anti-inflammatory drug wa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Louthrenoo W,Nilganuwong S,Aksaranugraha S,Asavatanabodee P,Saengnipanthkul S,Thai Study Group.

    更新日期:2007-06-01 00:00:00

  • Long-term effectiveness of exercise therapy in patients with osteoarthritis of the hip or knee: a randomized controlled trial comparing two different physical therapy interventions.

    abstract:OBJECTIVE:To determine if behavioral graded activity (BGA) results in better long-term effectiveness (5 years after inclusion) than usual exercise therapy (UC; usual care) in patients with osteoarthritis (OA) of the hip or knee. METHOD:Long-term follow-up study of a single blind cluster randomized trial comparing BGA ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Pisters MF,Veenhof C,Schellevis FG,De Bakker DH,Dekker J

    更新日期:2010-08-01 00:00:00

  • Fluorophore-assisted carbohydrate electrophoresis (FACE) of glycosaminoglycans.

    abstract:OBJECTIVE:Quantitation and analyses of the fine structure of glycosaminoglycans are increasingly important for understanding many biological processes, including those most critical for understanding skeletal biology. We have developed a novel procedure, fluorophore-assisted carbohydrate electrophoresis (FACE), for det...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Calabro A,Midura R,Wang A,West L,Plaas A,Hascall VC

    更新日期:2001-01-01 00:00:00

  • Preoperative progressive explosive-type resistance training is feasible and effective in patients with hip osteoarthritis scheduled for total hip arthroplasty--a randomized controlled trial.

    abstract:OBJECTIVE:To investigate the efficacy and feasibility of progressive explosive-type resistance training (RT) in patients with osteoarthritis (OA) of the hip scheduled for total hip arthroplasty (THA). METHOD:Randomized controlled trial (1:1) in patients diagnosed with hip OA and scheduled for THA. The intervention gro...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Hermann A,Holsgaard-Larsen A,Zerahn B,Mejdahl S,Overgaard S

    更新日期:2016-01-01 00:00:00

  • WOMAC Osteoarthritis Index--additional dimensions for use in subjects with post-traumatic osteoarthritis of the knee. Western Ontario and MacMaster Universities.

    abstract:OBJECTIVE:To compare the sensitivity of WOMAC and the two added dimensions Sport and Recreation Function and Knee Related Quality of Life in subjects with radiographic knee OA to that in controls. To study the influence of age on the reported outcomes. DESIGN:Outcomes were compared between subjects having had meniscec...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Roos EM,Roos HP,Lohmander LS

    更新日期:1999-03-01 00:00:00

  • Association between smoking and risk of knee osteoarthritis: a systematic review and meta-analysis.

    abstract:OBJECTIVE:To investigate the association between smoking and the risk for knee osteoarthritis (OA). DESIGN:Cohort, case-control, and cross-sectional studies were obtained from the Medline, Embase, and Web of Science databases. Estimates were calculated using a random-effects model. Subgroup analyses and meta-regressio...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,meta分析,评审


    authors: Kong L,Wang L,Meng F,Cao J,Shen Y

    更新日期:2017-06-01 00:00:00

  • Establishment of a bipotent cell line CL-1 which differentiates into chondrocytes and adipocytes from adult mouse.

    abstract:OBJECTIVE:Establishment of a clonal bipotent chondroprogenitor cell line from adult mouse to provide a new tool for the elucidation of chondrogenesis in adult animal. DESIGN:A clonal cell line CL-1 was established from tibia of adult mouse. Differentiation of CL-1 was characterized in monolayer culture. Effects of gro...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kitamura H

    更新日期:2004-01-01 00:00:00

  • Systematic review of non-surgical therapies for osteoarthritis of the hand: an update.

    abstract:OBJECTIVE:To update our earlier systematic review which evaluated all published randomized controlled trials (RCTs) evaluating pharmacological and non-pharmacological therapies in patients with hand osteoarthritis (OA). Surgical therapies were not evaluated. METHOD:RCTs published between August 2004 and February 2008 ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Mahendira D,Towheed TE

    更新日期:2009-10-01 00:00:00

  • Synergistic effect of IGF-1 and OP-1 on matrix formation by normal and OA chondrocytes cultured in alginate beads.

    abstract:OBJECTIVE:Growth factor therapy may be useful for stimulation of cartilage matrix synthesis and repair. Thus, the purpose of our study was to further understand the effect of combined insulin-like growth factor-1 (IGF-1) and osteogenic protein-1 (OP-1) treatment on the matrix synthesized by human adult normal and osteo...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Chubinskaya S,Hakimiyan A,Pacione C,Yanke A,Rappoport L,Aigner T,Rueger DC,Loeser RF

    更新日期:2007-04-01 00:00:00

  • A meta-analysis of sex differences prevalence, incidence and severity of osteoarthritis.

    abstract:OBJECTIVE:To resolve uncertainty regarding sex differences in osteoarthritis (OA) by performing a meta-analysis of sex differences in OA prevalence, incidence and severity. METHODS:Standard search strategies for population-based studies of OA providing sex-specific data. Random effects meta-analysis to provide pooled ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,meta分析


    authors: Srikanth VK,Fryer JL,Zhai G,Winzenberg TM,Hosmer D,Jones G

    更新日期:2005-09-01 00:00:00

  • The chemokine receptor CCR5 plays a role in post-traumatic cartilage loss in mice, but does not affect synovium and bone.

    abstract:OBJECTIVE:C-C chemokine receptor type 5 (CCR5) has been implicated in rheumatoid arthritis and several inflammatory diseases, where its blockade resulted in reduced joint destruction. However, its role in modulating cartilage and bone changes in post-traumatic osteoarthritis (OA) has not yet been investigated. In this ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Takebe K,Rai MF,Schmidt EJ,Sandell LJ

    更新日期:2015-03-01 00:00:00

  • Human genome-wide expression analysis reorients the study of inflammatory mediators and biomechanics in osteoarthritis.

    abstract::A major objective of this article is to examine the research implications of recently available genome-wide expression profiles of cartilage from human osteoarthritis (OA) joints. We propose that, when viewed in the light of extensive earlier work, this novel data provides a unique opportunity to reorient the design o...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Sandy JD,Chan DD,Trevino RL,Wimmer MA,Plaas A

    更新日期:2015-11-01 00:00:00

  • Natural history of cartilage damage and osteoarthritis progression on magnetic resonance imaging in a population-based cohort with knee pain.

    abstract:OBJECTIVES:To determine the natural history of cartilage damage and of osteoarthritis (OA) progression using magnetic resonance imaging (MRI); to evaluate whether OA progression varies by stage of disease. METHODS:A population-based cohort with knee pain was assessed clinically, with X-ray (Kellgren-Lawrence [KL] grad...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Cibere J,Sayre EC,Guermazi A,Nicolaou S,Kopec JA,Esdaile JM,Thorne A,Singer J,Wong H

    更新日期:2011-06-01 00:00:00

  • Comparative pain reduction of oral non-steroidal anti-inflammatory drugs and opioids for knee osteoarthritis: systematic analytic review.

    abstract:OBJECTIVE:Summarize the comparative effectiveness of oral non-steroidal anti-inflammatory drugs (NSAIDs) and opioids in reducing knee osteoarthritis (OA) pain. METHODS:Two reviewers independently screened reports of randomized controlled trials (RCTs), published in English between 1982 and 2015, evaluating oral NSAIDs...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Smith SR,Deshpande BR,Collins JE,Katz JN,Losina E

    更新日期:2016-06-01 00:00:00

  • The effects of radial shock waves on gene transfer in rabbit chondrocytes in vitro.

    abstract:OBJECTIVE:The purpose of this study was to develop a new technique of gene transfer utilizing radial shock waves. The effects of radial shock waves on gene transfer in rabbit chondrocytes were examined by varying the parameters of exposure conditions in vitro. METHODS:Chondrocytes were obtained from New Zealand white ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Murata R,Nakagawa K,Ohtori S,Ochiai N,Arai M,Saisu T,Sasho T,Takahashi K,Moriya H

    更新日期:2007-11-01 00:00:00