当前位置： SCI文献检索 > OSTEOARTHRITIS AND CARTILAGE期刊下所有文献 > Expression of biglycan, decorin and fibromodulin in the hypertrophic phase of experimental osteoarthritis.

Expression of biglycan, decorin and fibromodulin in the hypertrophic phase of experimental osteoarthritis.

Abstract：

:This study sought to assess the relative levels of the mRNAs of the core proteins of the small proteoglycans (PGs) biglycan, decorin and fibromodulin in the hypertrophic phase of the early osteoarthritis (OA) that follows joint injury. Experimental OA was induced in eight dogs by transection of the anterior cruciate ligament. Articular cartilage was harvested from each joint, the total RNA was extracted and the concentration of DNA in the cartilage was measured. The relative levels of mRNA for biglycan, decorin and fibromodulin were assessed by northern blot analyses. An increase in cartilage mass with no increase in DNA concentration confirmed that the joints were in the hypertrophic phase that follows joint injury. The total RNA per microgram of DNA was increased 2.5 times. Compared with control cartilage, the mRNA levels in osteoarthritic cartilage, when normalized to the concentration of DNA, were increased 3.9 times for biglycan, 1.2 times for decorin and 2.4 times for fibromodulin. Because these small PGs affect collagen fibrillogenesis in vitro, their discoordinate metabolism may contribute to the abnormal collagen formation and deposition that occurs in OA and to the ultimate failure of the articular cartilage.

journal_name

Osteoarthritis Cartilage

journal_title

Osteoarthritis and cartilage

authors

Dourado GS,Adams ME,Matyas JR,Huang D

doi

10.1016/s1063-4584(96)80015-x

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

187-96

issue

3

eissn

1063-4584

issn

1522-9653

pii

S1063-4584(96)80015-X

journal_volume

4

pub_type

杂志文章

相关文献

OSTEOARTHRITIS AND CARTILAGE文献大全
  • NEL-like molecule-1-modified bone marrow mesenchymal stem cells/poly lactic-co-glycolic acid composite improves repair of large osteochondral defects in mandibular condyle.

    abstract：OBJECTIVE:Articular cartilage of the mandibular condyle has limited ability to regenerate itself after injury. This study was to investigate whether osteochondral defects in mandibular condyle could be repaired by NELL-1(NEL-like molecule-1)-modified autogenous bone marrow mesenchymal stem cells (BMMSCs) and poly lacti...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2011.02.015

    authors： Zhu S,Zhang B,Man C,Ma Y,Hu J

    更新日期：2011-06-01 00:00:00

  • Effectiveness of autologous chondrocyte implantation in cartilage repair of the knee: a systematic review of controlled trials.

    abstract：OBJECTIVE:The relative differences in effectiveness of subchondral stimulation, osteochondral grafts, and autologous chondrocyte implantation (ACI) are still unclear. It is the objective of this study to systematically review the literature on ACI compared to other treatments by clinical outcome and the quality of the ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2010.03.005

    authors： Vavken P,Samartzis D

    更新日期：2010-06-01 00:00:00

  • Radiographic progression of knee osteoarthritis is associated with MRI abnormalities in both the patellofemoral and tibiofemoral joint.

    abstract：OBJECTIVE:To investigate patterns of MRI abnormalities in the patellofemoral (PFJ) and tibiofemoral joint (TFJ) and their association with radiographic progression, using hypothesis free analyses. DESIGN:205 patients from the GARP study with symptomatic OA at multiple sites (mean age 60 years, 80% woman, median BMI 26...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2015.09.021

    authors： de Lange-Brokaar BJ,Bijsterbosch J,Kornaat PR,Yusuf E,Ioan-Facsinay A,Zuurmond AM,Kroon HM,Meulenbelt I,Bloem JL,Kloppenburg M

    更新日期：2016-03-01 00:00:00

  • Transcriptional induction of SOX9 by NF-kappaB family member RelA in chondrogenic cells.

    abstract：OBJECTIVE:Although SOX9 is a key molecule for chondrogenic differentiation, little is known about the upstream signal. The present study attempted to identify transcription factors to induce SOX9 expression and examined the mechanism. METHODS:Sequences of about 1 kb of 5'-end flanking regions were compared between hum...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2009.02.003

    authors： Ushita M,Saito T,Ikeda T,Yano F,Higashikawa A,Ogata N,Chung U,Nakamura K,Kawaguchi H

    更新日期：2009-08-01 00:00:00

  • Overexpression of active TGF-beta-1 in the murine knee joint: evidence for synovial-layer-dependent chondro-osteophyte formation.

    abstract：OBJECTIVE:To investigate the impact of a prolonged and constant active TGF-beta expression by the synovial lining cells on cartilage and ligamentous joint structures in vivo. DESIGN:An adenoviral vector (AdTGF-beta1(223,225)) was used for the overexpression of active TGF-beta1 in knee joints of C57Bl/6 mice. RESULTS:...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2000.0368

    authors： Bakker AC,van de Loo FA,van Beuningen HM,Sime P,van Lent PL,van der Kraan PM,Richards CD,van den Berg WB

    更新日期：2001-02-01 00:00:00

  • Trabecular bone structure and spatial differences in articular cartilage MR relaxation times in individuals with posterior horn medial meniscal tears.

    abstract：OBJECTIVE:To analyze knee trabecular bone structure and spatial cartilage T(1ρ) and T(2) relaxation times using 3-T magnetic resonance imaging (MRI) in subjects with and without tears of posterior horn of the medial meniscus (PHMM). DESIGN:3-T MRI from 59 subjects (>18 years), were used to evaluate PHMM tears based on...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2012.09.014

    authors： Kumar D,Schooler J,Zuo J,McCulloch CE,Nardo L,Link TM,Li X,Majumdar S

    更新日期：2013-01-01 00:00:00

  • The innate immune response as a mediator of osteoarthritis pain.

    abstract：:In this narrative review, we discuss the emerging role of innate immunity in osteoarthritis (OA) joint pain. First, we give a brief description of the pain pathway in the context of OA. Then we consider how neuro-immune signaling pathways may promote OA pain. First, activation of neuronal Pattern Recognition Receptors...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2019.11.006

    authors： Miller RJ,Malfait AM,Miller RE

    更新日期：2020-05-01 00:00:00

  • Site specific changes in gene expression and cartilage metabolism during early experimental osteoarthritis.

    abstract：OBJECTIVES:To characterize the molecular events underlying cartilage injury in the early phase of mono-iodoacetate-induced osteoarthritis (OA) in rats. METHODS:Experimental osteoarthritis was induced by intra-articular injection of 0.03mg mono-iodoacetate (MIA) in Wistar rats. Animals were killed 2, 5, 10, 15 and 20 d...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2003.11.008

    authors： Dumond H,Presle N,Pottie P,Pacquelet S,Terlain B,Netter P,Gepstein A,Livne E,Jouzeau JY

    更新日期：2004-04-01 00:00:00

  • Gene expression profiling following BMP-2 induction of mesenchymal chondrogenesis in vitro.

    abstract：OBJECTIVE:This study aims to apply gene expression profiling technology to gain insight into the molecular regulation of mesenchymal chondrogenesis. METHODS:The experimental system consists of micromass cultures of C3H10T1/2 cells, a murine multipotential embryonic cell line, treated with the chondroinductive growth f...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2001.0478

    authors： Izzo MW,Pucci B,Tuan RS,Hall DJ

    更新日期：2002-01-01 00:00:00

  • Osteoarthritis year 2010 in review: pathomechanisms.

    abstract：:Osteoarthritis is characterized by progressive breakdown of articular cartilage. This review summarizes findings of the last year, which shed new light on mechanisms and factors involved in cartilage loss. Evidence is accumulating that the transcription factor hypoxia-inducible factor-2α (HIF-2α) is highly enhanced in...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2011.01.022

    authors： van den Berg WB

    更新日期：2011-04-01 00:00:00

  • Differential response of porcine osteoblasts and chondrocytes in cell or tissue culture after 5-aminolevulinic acid-based photodynamic therapy.

    abstract：OBJECTIVE:Outcome in osteochondral allografting is limited by the immunological incompatibility of the grafted tissue. Based on a resistance of chondrocytes to photodynamic therapy in cell culture it is proposed that 5-aminolevulinic acid-based photodynamic therapy (5-ALA-PDT) might be used to inactivate bone while mai...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2008.08.005

    authors： Bastian JD,Egli RJ,Ganz R,Hofstetter W,Leunig M

    更新日期：2009-04-01 00:00:00

  • Mechanical properties of articular cartilage covered by the meniscus.

    abstract：OBJECTIVE:To investigate the mechanical properties and morphological characteristics of articular cartilage on the tibial plateau of human knees, including the region covered by the meniscus. DESIGN:Using a 1-mm diameter flat-ended cylindrical probe to apply a constant load (0.6 MPa) at specific sites on the tibial pl...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2006.01.015

    authors： Thambyah A,Nather A,Goh J

    更新日期：2006-06-01 00:00:00

  • Mechanism of proteoglycan aggregate degradation in cartilage stimulated with oncostatin M.

    abstract：OBJECTIVE:To investigate the potential synergistic and differential effects of cytokine combinations on proteoglycan aggregate catabolism in cartilage. METHODS:Bovine articular cartilage explants were maintained in organ culture and subjected to stimulation with cytokine combinations including interleukin-1alpha (IL-1...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.05.002

    authors： Durigova M,Roughley PJ,Mort JS

    更新日期：2008-01-01 00:00:00

  • Intermittent cyclic loading of cartilage explants modulates fibronectin metabolism.

    abstract：OBJECTIVE:The aim of this study was to evaluate systematically the effect of tissue load, its amplitude, time of intermittence and duration of loading on the biosynthesis and release of fibronectin by intermittently loaded mature bovine articular cartilage explants. METHODS:Cyclic compressive pressure was introduced u...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(97)80037-4

    authors： Steinmeyer J,Ackermann B,Raiss RX

    更新日期：1997-09-01 00:00:00

  • Modulation of bovine articular chondrocyte gene expression in vitro by oxygen tension.

    abstract：OBJECTIVE:Adult articular cartilage is a physiologically hypoxic tissue with a proposed gradient of oxygen tension ranging from <10% oxygen at the cartilage surface to <1% in the deepest layers. This gradient may be disturbed during diseases of the joint, for example in rheumatoid arthritis when synovial fluid pO(2)fal...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2000.0396

    authors： Grimshaw MJ,Mason RM

    更新日期：2001-05-01 00:00:00

  • Notch signaling in postnatal joint chondrocytes, but not subchondral osteoblasts, is required for articular cartilage and joint maintenance.

    abstract：OBJECTIVE:Notch signaling has been identified as a critical regulator in cartilage development and joint maintenance, and loss of Notch signaling in all joint tissues results in an early and progressive osteoarthritis (OA)-like pathology. This study investigated the targeted cell population within the knee joint in whi...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2015.10.015

    authors： Liu Z,Ren Y,Mirando AJ,Wang C,Zuscik MJ,O'Keefe RJ,Hilton MJ

    更新日期：2016-04-01 00:00:00

  • The anti-NGF antibody muMab 911 both prevents and reverses pain behaviour and subchondral osteoclast numbers in a rat model of osteoarthritis pain.

    abstract：OBJECTIVE:Nerve growth factor (NGF) has a pivotal role in peripheral hyperalgesia and inflammation; anti-NGF antibodies attenuate pain responses in inflammatory pain models, and in people with osteoarthritis (OA) or low back pain. The aim of this study was to characterise the peripheral mechanisms contributing to the a...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.05.015

    authors： Xu L,Nwosu LN,Burston JJ,Millns PJ,Sagar DR,Mapp PI,Meesawatsom P,Li L,Bennett AJ,Walsh DA,Chapman V

    更新日期：2016-09-01 00:00:00

  • Gene expression profiling of chondrocytes from a porcine impact injury model.

    abstract：OBJECTIVE:To identify differentially expressed genes between axially impacted and control articular cartilage taken from porcine patellae maintained in organ culture for 14 days. METHODS:Porcine patellae were impacted perpendicular to the articular surface to create an impact injury. Intact patellae (control and impac...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.12.012

    authors： Ashwell MS,O'Nan AT,Gonda MG,Mente PL

    更新日期：2008-08-01 00:00:00

  • Clinical feasibility of a novel biphasic osteochondral composite for matrix-associated autologous chondrocyte implantation.

    abstract：OBJECTIVE:Matrix-associated autologous chondrocyte implantation has been used to treat cartilage defects. We developed a biphasic cylindrical osteochondral composite construct for such use, and conducted this study to determine its feasibility for treating osteochondral lesions in human knees. METHOD:Ten patients with...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.01.004

    authors： Chiang H,Liao CJ,Hsieh CH,Shen CY,Huang YY,Jiang CC

    更新日期：2013-04-01 00:00:00

  • Neo-cartilage engineered from primary chondrocytes is epigenetically similar to autologous cartilage, in contrast to using mesenchymal stem cells.

    abstract：OBJECTIVES:To compare the epigenetic landscape of 3D cell models of human primary articular chondrocytes (hPACs) and human bone-marrow derived mesenchymal stem cells (hBMSCs) and their respective autologous articular cartilage. DESIGN:Using Illumina Infinium HumanMethylation450 BeadChip arrays, the DNA methylation lan...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.03.009

    authors： Bomer N,den Hollander W,Suchiman H,Houtman E,Slieker RC,Heijmans BT,Slagboom PE,Nelissen RG,Ramos YF,Meulenbelt I

    更新日期：2016-08-01 00:00:00

  • The control of cell division in articular chondrocytes.

    abstract：:Our objective was to investigate the factors that control chondrocyte division, namely, (1) steric hindrance caused by the surrounding matrix, (2) growth factors and (3) cell shape. Bovine articular cartilage explants and isolated chondrocytes were cultured in medium containing [3H]-TdR to measure DNA synthesis. The e...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(05)80029-9

    authors： Lee DA,Bentley G,Archer CW

    更新日期：1993-04-01 00:00:00

  • Reliability, reproducibility and variability of the traditional Histologic/Histochemical Grading System vs the new OARSI Osteoarthritis Cartilage Histopathology Assessment System.

    abstract：OBJECTIVE:For many years, the Histologic/Histochemical Grading System (HHGS) for osteoarthritis monitoring has been used as a histological scoring system for the quality of cartilage. There are, however, some limitations using this grading system. The goal of the investigation presented in this paper was to examine the...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.04.017

    authors： Custers RJ,Creemers LB,Verbout AJ,van Rijen MH,Dhert WJ,Saris DB

    更新日期：2007-11-01 00:00:00

  • Combined effects of growth factors and static mechanical compression on meniscus explant biosynthesis.

    abstract：OBJECTIVE:To compare the actions of fibroblast growth factor-basic (bFGF), insulin-like growth factor-I (IGF-I), platelet derived growth factor-AB (PDGF-AB), and transforming growth factor-beta 1 (TGF-beta1) on bovine meniscus tissue explants with and without static mechanical compression. DESIGN:Meniscus tissue expla...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2004.05.007

    authors： Imler SM,Doshi AN,Levenston ME

    更新日期：2004-09-01 00:00:00

  • Expression of the semicarbazide-sensitive amine oxidase in articular cartilage: its role in terminal differentiation of chondrocytes in rat and human.

    abstract：OBJECTIVE:Semicarbazide-sensitive amine oxidase (SSAO) catalyzes the oxidation of primary amines into ammonia and reactive species (hydrogen peroxide, aldehydes). It is highly expressed in mammalian tissues, especially in vascular smooth muscle cells and adipocytes, where it plays a role in cell differentiation and glu...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.01.340

    authors： Filip A,Pinzano A,Bianchi A,Fève B,Jalkanen S,Gillet P,Mainard D,Lacolley P,Magdalou J,Mercier N

    更新日期：2016-07-01 00:00:00

  • The response of elderly human articular cartilage to mechanical stimuli in vitro.

    abstract：OBJECTIVE:To investigate the biosynthetic response of elderly human femoral head articular cartilage to mechanical stimulation in vitro and its variation with site. METHOD:Full-depth cartilage biopsies of articular cartilage were removed from defined sites on 10 femoral heads from patients aged 68-95 years. Cartilage ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2005.07.002

    authors： Plumb MS,Aspden RM

    更新日期：2005-12-01 00:00:00

  • Can anatomic alignment measured from a knee radiograph substitute for mechanical alignment from full limb films?

    abstract：OBJECTIVES:To examine whether categories of anatomic alignment (varus, neutral, valgus) measured from knee X-rays agree with similar categories of mechanical alignment from the full limb film and whether varus anatomic malalignment predicts medial joint space loss on knee X-rays as well as varus mechanical alignment. ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2009.05.012

    authors： Felson DT,Cooke TD,Niu J,Goggins J,Choi J,Yu J,Nevitt MC,OAI Investigators Group.

    更新日期：2009-11-01 00:00:00

  • Inhibition of LRRK2 restores parkin-mediated mitophagy and attenuates intervertebral disc degeneration.

    abstract：OBJECTIVE:To elucidate the role of LRRK2 in intervertebral disc degeneration (IDD) as well as its mitophagy regulation mechanism. METHODS:The expression of LRRK2 in human degenerative nucleus pulposus tissues as well as in oxidative stress-induced rat nucleus pulposus cells (NPCs) was detected by western blot. LRRK2 w...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2021.01.002

    authors： Lin J,Zheng X,Zhang Z,Zhuge J,Shao Z,Huang C,Jin J,Chen X,Chen Y,Wu Y,Tian N,Sun L,Gao W,Zhou Y,Wang X,Zhang X

    更新日期：2021-01-09 00:00:00

  • Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cartilage.

    abstract：OBJECTIVE:The discovery of small molecular compounds that expand cartilage is needed. We searched for small molecular compounds that expand cartilage or enhance the actions of bone morphogenetic proteins (BMPs) on cartilage. DESIGN:Metatarsal primordial cartilage explants prepared from 14.5 days postcoitum (d.p.c.) mo...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2010.11.007

    authors： Ikegami D,Iwai T,Ryo S,Gu N,Sugiyama T,Oh I,Yoshikawa H,Tsumaki N

    更新日期：2011-02-01 00:00:00

  • Osteoarthritis year in review 2017: updates on imaging advancements.

    abstract：OBJECTIVE:This narrative review covers original research publications related to imaging advancements in osteoarthritis (OA) published in the English language between 1st April 2016 and 30th April 2017. METHODS:Relevant human studies (excluding pre-clinical and in vitro studies), were searched and selected from PubMed...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2018.01.007

    authors： Hafezi-Nejad N,Demehri S,Guermazi A,Carrino JA

    更新日期：2018-03-01 00:00:00

  • Purine receptors modulate chondrocyte extracellular inorganic pyrophosphate production.

    abstract：OBJECTIVE:Extracellular inorganic pyrophosphate (ePPi) plays a key role in the regulation of normal and pathologic mineralization. The purpose of this work was to evaluate the role of P1 and P2 purine receptors in modulating ePPi production by articular chondrocytes. METHODS:Porcine cartilage explants and chondrocyte ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2010.08.004

    authors： Rosenthal AK,Hempel D,Kurup IV,Masuda I,Ryan LM

    更新日期：2010-11-01 00:00:00