Abstract：
OBJECTIVE:In the current studies we have examined the effects of nitric oxide, and its redox derivatives peroxynitrite and S-nitrosothiol, S-nitrosocysteine, on nuclear factor kappaB (NF-kappaB) activation in cytokine-stimulated bovine chondrocytes. METHODS:The kinetics of NF-kappaB activation (p65 nuclear translocation) were assessed by immunofluorescence and immunoblot assays. RESULTS:We observed that the two nitric oxide redox species, peroxynitrite and S-nitrosocysteine, exert opposing effects on NF-kappaB activation. However, in lipopolysaccharide (LPS)/cytokine-stimulated chondrocytes (LPS, IL-1beta and TNF-alpha (LIT)) in the presence or absence of the NOS inhibitor L-NG-monomethyl arginine citrate (L-NMMA), the results indicate that nitric oxide causes persistent activation of NF-kappaB, most likely via generation of the free radical derivative peroxynitrite. CONCLUSION:The studies indicate that while nitric oxide is not required for immediate NF-kappaB activation in cytokine-stimulated chondrocytes, its effect is to sustain nuclear translocation of p65 and thereby provide a persistent "on signal" to NF-kappaB dependent gene transcription. Persistent activation of NF-kappaB may represent a mechanism by which nitric oxide sustains catabolic processes and promotes cartilage degeneration in osteoarthritis.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Clancy RM,Gomez PF,Abramson SBdoi
10.1016/j.joca.2004.04.003keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
552-8issue
7eissn
1063-4584issn
1522-9653pii
S1063458404000640journal_volume
12pub_type
杂志文章abstract：OBJECTIVE:One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally marked using green fluor...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2001.0486
更新日期：2002-02-01 00:00:00
abstract：OBJECTIVE:Abnormal metabolic activities of chondrocytes may cause articular cartilage (AC) degradation, but key transcription factors regulating metabolic activities in AC of aging individuals remain unknown. This study aimed to investigate the role of transcription factor NFAT1 in regulating the expression of anabolic...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.02.003
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:Here we investigate whether monolayer culture or culture at 21% oxygen influences activity of cytochrome c oxidase, the terminal enzyme in the respiratory chain whose activity is essential for oxidative metabolism and whether return to three dimensional (3-D) culture restores cytochrome c oxidase activity to ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2009.03.001
更新日期：2009-08-01 00:00:00
abstract：OBJECTIVE:To investigate the impact of a prolonged and constant active TGF-beta expression by the synovial lining cells on cartilage and ligamentous joint structures in vivo. DESIGN:An adenoviral vector (AdTGF-beta1(223,225)) was used for the overexpression of active TGF-beta1 in knee joints of C57Bl/6 mice. RESULTS:...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2000.0368
更新日期：2001-02-01 00:00:00
abstract：PURPOSE:Our aim was to introduce a simplified MRI instrument, Rapid OsteoArthritis MRI Eligibility Score (ROAMES), for defining structural eligibility of patients for inclusion in disease-modifying osteoarthritis drug trials using a tri-compartmental anatomic approach that enables stratification of knees into different...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2019.08.005
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:A recent genome-wide association study reported significant associations of genetic variants within the ALDH1A2 gene with osteoarthritis (OA) of the hand in European populations. However, these findings have not been well generalized to other joints, or to other populations. METHODS:We performed a two-stage ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2017.01.003
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy and safety of chondroitin sulfate and/or glucosamine hydrochloride in alleviating symptoms and improving the dysfunction of Kashin-Beck disease (KBD) patients. METHODS:We undertook a cluster-randomized, placebo-controlled trial in 251 patients with KBD. Participants were randomly all...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joca.2012.03.013
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVE:This study was performed to non-invasively visualize and characterize osteochondral (OC) repair in ex vivo goat stifles using an 8 T magnetic resonance imaging (MRI) scanner and to compare the MR morphology with images obtained from 1.5 T, gross morphology and histology. METHODS:Mature, neutered male goats w...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2005.08.006
更新日期：2006-01-01 00:00:00
abstract：:To highlight methodological challenges in the design and conduct of randomized trials of surgical interventions and to propose strategies for addressing these challenges. This paper focuses on three broad areas: enrollment; intervention; and assessment including implications for analysis. For each challenge raised in ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2015.02.024
更新日期：2015-05-01 00:00:00
abstract：OBJECTIVE:For many years, the Histologic/Histochemical Grading System (HHGS) for osteoarthritis monitoring has been used as a histological scoring system for the quality of cartilage. There are, however, some limitations using this grading system. The goal of the investigation presented in this paper was to examine the...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.04.017
更新日期：2007-11-01 00:00:00
abstract：OBJECTIVE:This study investigated the in vitro effects of hyaluronic acid (HA) of molecular weight (MW) 500-730 kDa on human articular chondrocytes cultivated for 48 h in the presence of interleukin-1beta (IL-1beta) with and without hydrostatic cyclical pressure. DESIGN:The effects of 10 and 100 microg/ml HA with and ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2005.03.006
更新日期：2005-08-01 00:00:00
abstract：OBJECTIVE:Previous longitudinal studies have shown no associations between increasing amount of radiographic hand osteoarthritis (OA) and levels of hand pain/disability. In this longitudinal study, we aimed to study whether radiographic hand OA was related to pain/disability in cross-sectional and longitudinal settings...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2013.04.004
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:The objectives for this study were to determine whether radical initiated photopolymerizations typically employed for cell encapsulations lead to oxidative stress incurred by chondrocytes and whether the development of a pericellular matrix (PCM) decreases this oxidative stress and has longer-term benefits on...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2012.06.015
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVE:To assess the effectiveness of short-wave diathermy (SWD) treatment in the management of knee osteoarthritis (KOA) and to assess whether the effects are related to the induction of a thermal effect. METHODS:A systematic literature search was conducted in PubMed, CINAHL, PEDro, EMBASE, SPORTdiscus and Scholar...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.joca.2012.05.005
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVE:To identify the prevalence of sarcopenic obesity, a phenotype of low muscle mass and high adiposity, in adults with end-stage knee osteoarthritis (OA). Various diagnostic criteria, including assessment of muscle/fat mass, muscle strength and physical function, were used to identify patients with and without s...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2019.05.026
更新日期：2019-12-01 00:00:00
abstract：OBJECTIVE:To characterise in vitro engineered cartilaginous constructs made employing a novel static, scaffold-free and closed chamber system. DESIGN:Chondrocytes derived from slaughter age pigs (3-6 months) were seeded at high density (20 x 10(6)) into cylindrical chambers (1.0 x 0.5cm) and were maintained between an...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(03)00053-0
更新日期：2003-06-01 00:00:00
abstract：OBJECTIVE:To elucidate variations in tissue ultrastructure and incidence of pathology between fibromodulin (FM)-null mice and wild-type (WT) animals. DESIGN:FM-null and WT siblings from different age groups were compared. Serial sections were made through paraffin-embedded whole knees and investigated histologically. ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2002.0527
更新日期：2002-10-01 00:00:00
abstract：OBJECTIVE:Glucosamine is commonly used for the treatment of osteoarthritis, and its use is increasing in the general population. The Canadian Multicentre Osteoporosis Study (CaMos) provided an opportunity to examine the prevalence of glucosamine use across age and gender groups, and to assess the factors associated wit...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,多中心研究
doi：10.1016/j.joca.2006.06.003
更新日期：2006-12-01 00:00:00
abstract：OBJECTIVE:To investigate the in vivo role of the IRE1/XBP1 unfolded protein response (UPR) signaling pathway in cartilage. DESIGN:Xbp1(flox/flox).Col2a1-Cre mice (Xbp1(CartΔEx2)), in which XBP1 activity is ablated specifically from cartilage, were analyzed histomorphometrically by Alizarin red/Alcian blue skeletal pre...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.01.001
更新日期：2015-04-01 00:00:00
abstract：OBJECTIVE:To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice. METHODS:Col2a1-CreER(T2) transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER(T2);R26R mice were administered TM for 5 day...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.08.001
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVE:To investigate individual preferences for physical activity (PA) attributes in adults with chronic knee pain, to identify clusters of individuals with similar preferences, and to identify whether individuals in these clusters differ by their demographic and health characteristics. DESIGN:An adaptive conjoint...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,多中心研究
doi：10.1016/j.joca.2018.10.002
更新日期：2019-02-01 00:00:00
abstract：OBJECTIVE:Substantial changes in articular cartilage composition and mechanical properties occur during the development of osteoarthritis (OA). While softening in the initial stage is reported and sometimes used as an indicator of early OA, there is a lack of data relating the macroscopic appearance of cartilage to its...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2005.06.008
更新日期：2005-11-01 00:00:00
abstract：OBJECTIVE:Adult articular cartilage is a physiologically hypoxic tissue with a proposed gradient of oxygen tension ranging from <10% oxygen at the cartilage surface to <1% in the deepest layers. This gradient may be disturbed during diseases of the joint, for example in rheumatoid arthritis when synovial fluid pO(2)fal...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2000.0396
更新日期：2001-05-01 00:00:00
abstract：OBJECTIVE:To determine the interrelations between degenerative changes in articular cartilage and underlying trabecular bone during development of osteoarthritis and to test the ability of quantitative magnetic resonance imaging (MRI) to detect those changes. METHODS:Human cadaver patellae were investigated with quant...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.03.019
更新日期：2007-10-01 00:00:00
abstract：OBJECTIVE:The objective of this work was to compare the measurement properties of three categorical X-ray scoring methods for hip osteoarthritis (OA). METHODS:In data obtained from trials and cohorts, radiographs were evaluated using the Kellgren and Lawrence (KL) grading system, the Osteoarthritis Research Society In...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2008.06.009
更新日期：2009-02-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate systematically the effect of tissue load, its amplitude, time of intermittence and duration of loading on the biosynthesis and release of fibronectin by intermittently loaded mature bovine articular cartilage explants. METHODS:Cyclic compressive pressure was introduced u...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(97)80037-4
更新日期：1997-09-01 00:00:00
abstract：OBJECTIVES:The delayed Gadolinium-Enhanced MRI of Cartilage (dGEMRIC) technique is a method proposed for non-invasive measurement of cartilage glycosaminoglycan (GAG) content. In this method, gadopentetate (Gd-DTPA²⁻) is assumed to distribute in cartilage in inverse relation to the GAG distribution, thus allowing quant...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2011.11.016
更新日期：2012-02-01 00:00:00
abstract：OBJECTIVES:Evaluate the prevalence of meeting the updated 2018 Physical Activity Guidelines for Americans (150 unbouted minutes in moderate-to-vigorous intensity physical activity [MVPA]) and determine cross-sectional factors associated with Guideline attainment in a community-based cohort of adults with or at elevated...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2020.03.007
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:Fetal cartilage anlage provides a framework for endochondral ossification and organization into articular cartilage. We previously reported differences between mechanical properties of talar cartilage anlagen and adult articular cartilage. However, the underlying development-associated changes remain to be es...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2011.07.013
更新日期：2011-10-01 00:00:00
abstract：OBJECTIVES:Low-dose radiation therapy (LDRT) is widely used as treatment for osteoarthritis (OA) in some countries, while relatively unknown in others. Systematic literature review displayed a lack of high-level evidence for beneficial effects in clinical practice. The aim was to assess the efficacy of LDRT on symptoms...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joca.2018.06.010
更新日期：2018-10-01 00:00:00
