当前位置： SCI文献检索 > OSTEOARTHRITIS AND CARTILAGE期刊下所有文献 > A static, closed and scaffold-free bioreactor system that permits chondrogenesis in vitro.

A static, closed and scaffold-free bioreactor system that permits chondrogenesis in vitro.

Abstract：

OBJECTIVE:To characterise in vitro engineered cartilaginous constructs made employing a novel static, scaffold-free and closed chamber system. DESIGN:Chondrocytes derived from slaughter age pigs (3-6 months) were seeded at high density (20 x 10(6)) into cylindrical chambers (1.0 x 0.5cm) and were maintained between an upper and a lower membrane (100 kDa) for 21 days and subsequently cultured in open culture for 7 additional days. RESULTS:Viable constructs produced were approximately 10 mmx2mm in size and were stable enough to be handled by surgical pincers. Histology and electron microscopy evaluations revealed a neo-cartilage structure of high cell density with a comprehensive extracellular matrix. Predominately collagen type II and negligible amounts of collagen types I and X were detected using RT-PCR and SDS-PAGE analyses. CONCLUSIONS:In this study, we provide evidence of a scaffold-free system that can produce immature hyaline-like cartilaginous constructs suitable for in vivo implantation, or that may be useful for in vitro studies of events related to the process of chondrogenesis.

journal_name

Osteoarthritis Cartilage

journal_title

Osteoarthritis and cartilage

authors

Grogan SP,Rieser F,Winkelmann V,Berardi S,Mainil-Varlet P

doi

10.1016/s1063-4584(03)00053-0

keywords:

subject

Has Abstract

pub_date

2003-06-01 00:00:00

pages

403-11

issue

6

eissn

1063-4584

issn

1522-9653

pii

S1063458403000530

journal_volume

11

pub_type

杂志文章

相关文献

OSTEOARTHRITIS AND CARTILAGE文献大全
  • Characterisation of the guinea pig model of osteoarthritis by in vivo three-dimensional magnetic resonance imaging.

    abstract：OBJECTIVE:To characterise longitudinal changes in joint integrity and cartilage volume in vivo in the guinea pig spontaneous osteoarthritis (OA) model by magnetic resonance imaging (MRI). METHODS:Guinea pigs knee were imaged in vivo by high-resolution three-dimensional (3D) MRI between the ages of 3 and 12 months. Ima...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(03)00162-6

    authors： Tessier JJ,Bowyer J,Brownrigg NJ,Peers IS,Westwood FR,Waterton JC,Maciewicz RA

    更新日期：2003-12-01 00:00:00

  • The contribution of hip geometry to the prediction of hip osteoarthritis.

    abstract：OBJECTIVE:To determine how well measures of hip geometry can predict radiological incident hip osteoarthritis (HOA) compared to well known clinical risk factors. DESIGN:The study population is part of the Rotterdam Study, a prospective population-based cohort. Baseline pelvic radiographs were used to measure hip geome...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.06.012

    authors： Castaño-Betancourt MC,Van Meurs JB,Bierma-Zeinstra S,Rivadeneira F,Hofman A,Weinans H,Uitterlinden AG,Waarsing JH

    更新日期：2013-10-01 00:00:00

  • Effects of a 10-week toe-out gait modification intervention in people with medial knee osteoarthritis: a pilot, feasibility study.

    abstract：OBJECTIVE:To examine the feasibility of a 10-week gait modification program in people with medial tibiofemoral knee osteoarthritis (OA), and to assess changes in clinical and biomechanical outcomes. DESIGN:Fifteen people with medial knee OA completed 10 weeks of gait modification focusing on increasing toe-out angle d...

    journal_title：Osteoarthritis and cartilage

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.joca.2014.04.007

    authors： Hunt MA,Takacs J

    更新日期：2014-07-01 00:00:00

  • Differences in trabecular bone texture between knees with and without radiographic osteoarthritis detected by directional fractal signature method.

    abstract：OBJECTIVE:To evaluate differences in tibial trabecular bone (TB) texture between subjects with and without radiographic knee osteoarthritis (OA) using a variance orientation transform (VOT) method. DESIGN:Subjects with knee OA (Kellgren & Lawrence grade > or =2) and controls without OA (both n=26, seven women) were ma...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2010.01.002

    authors： Wolski M,Podsiadlo P,Stachowiak GW,Lohmander LS,Englund M

    更新日期：2010-05-01 00:00:00

  • Subchondral bone in osteoarthritis: association between MRI texture analysis and histomorphometry.

    abstract：OBJECTIVE:Magnetic resonance imaging (MRI) texture analysis is a method of analyzing subchondral bone alterations in osteoarthritis (OA). The objective of this study was to evaluate the association between MR texture analysis and ground-truth subchondral bone histomorphometry at the tibial plateau. DESIGN:The local re...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.12.011

    authors： MacKay JW,Murray PJ,Kasmai B,Johnson G,Donell ST,Toms AP

    更新日期：2017-05-01 00:00:00

  • Change in serum measurements of cartilage oligomeric matrix protein and association with the development and worsening of radiographic hip osteoarthritis.

    abstract：OBJECTIVE:To determine the association between changes in serum levels of cartilage oligomeric matrix protein (COMP) and serum N-telopeptide crosslinks (NTX) over a 6-year interval with the development and progression of radiographically apparent hip osteoarthritis (RHOA) in a community sample of elderly women over 8.3...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.joca.2007.09.008

    authors： Chaganti RK,Kelman A,Lui L,Yao W,Javaid MK,Bauer D,Nevitt M,Lane NE,Study Of Osteoporotic Fractures Research Group Sof.

    更新日期：2008-05-01 00:00:00

  • Comparison of 1-year vs 2-year change in regional cartilage thickness in osteoarthritis results from 346 participants from the Osteoarthritis Initiative.

    abstract：OBJECTIVE:To compare femorotibial cartilage thickness changes over a 2- vs a 1-year observation period in knees with radiographic knee osteoarthritis (OA). METHODS:One knee of 346 Osteoarthritis Initiative (OAI) participants was studied at three time points [baseline (BL), year-1 (Y1), year-2 (Y2) follow-up]: 239 usin...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2010.10.022

    authors： Wirth W,Larroque S,Davies RY,Nevitt M,Gimona A,Baribaud F,Lee JH,Benichou O,Wyman BT,Hudelmaier M,Maschek S,Eckstein F,OA Initiative Investigators Group.

    更新日期：2011-01-01 00:00:00

  • Assessment of joint space narrowing with conventional standing antero-posterior radiographs: relief in mild-to-moderate pain is not a confounder in recent osteoarthritis structure-modifying drug trials.

    abstract：OBJECTIVE:Knee pain relief has been suggested to potentially alter radioanatomic positioning in conventional standing antero-posterior knee radiographs. This study was performed to determine whether this is always the case and in particular if it applied to two recent randomised, placebo-controlled trials showing both ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2006.02.022

    authors： Rovati LC,Pavelka K,Giacovelli G,Reginster JY

    更新日期：2006-01-01 00:00:00

  • Chondrocytes extract from patients with osteoarthritis induces chondrogenesis in infrapatellar fat pad-derived stem cells.

    abstract：OBJECTIVE:Infrapatellar fat pad of patients with osteoarthritis (OA) contains multipotent and highly clonogenic adipose-derived stem cells that can be isolated by low invasive methods. Moreover, nuclear and cytoplasmic cellular extracts have been showed to be effective in induction of cell differentiation and reprogram...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2012.10.007

    authors： López-Ruiz E,Perán M,Cobo-Molinos J,Jiménez G,Picón M,Bustamante M,Arrebola F,Hernández-Lamas MC,Delgado-Martínez AD,Montañez E,Marchal JA

    更新日期：2013-01-01 00:00:00

  • Chondroprotective effects of high-molecular-weight cross-linked hyaluronic acid in a rabbit knee osteoarthritis model.

    abstract：OBJECTIVES:We hypothesized that high-molecular-weight (MW) cross-linked (CL) hyaluronic acid (HA) improves joint lubrication and has an enhanced chondroprotective effect. We examined the histopathological changes and friction coefficients in osteoarthritic knee joints after injecting high-MW CL HA. DESIGN:A bilateral ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.10.005

    authors： Elmorsy S,Funakoshi T,Sasazawa F,Todoh M,Tadano S,Iwasaki N

    更新日期：2014-01-01 00:00:00

  • Electroporation-mediated gene transfer of SOX trio to enhance chondrogenesis in adipose stem cells.

    abstract：OBJECTIVE:The aim of the present study was to determine if the electroporation-mediated gene transfer of SOX trio enhances the chondrogenic potential of adipose stem cells (ASCs). DESIGN:ASCs were transfected with SOX trio genes using an electroporation technique and cultured for 3 weeks. The pellets were analyzed for...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2011.01.005

    authors： Im GI,Kim HJ

    更新日期：2011-04-01 00:00:00

  • Validity of joint space width measurements in hand osteoarthritis.

    abstract：OBJECTIVE:To investigate the validity of joint space width (JSW) measurements in millimeters (mm) in hand osteoarthritis (OA) patients by comparison to controls, grading of joint space narrowing (JSN), and clinical features. METHODS:Hand radiographs of 235 hand OA patients (mean age 65 years, 83% women) and 471 contro...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2011.08.011

    authors： Kwok WY,Bijsterbosch J,Malm SH,Biermasz NR,Huetink K,Nelissen RG,Meulenbelt I,Huizinga TW,van 't Klooster R,Stoel BC,Kloppenburg M

    更新日期：2011-11-01 00:00:00

  • Measurement properties of the WOMAC LK 3.1 pain scale.

    abstract：OBJECTIVE:The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) is applied extensively to patients with osteoarthritis of the hip or knee. Previous work has challenged the validity of its physical function scale however an extensive evaluation of its pain scale has not been reported. Our purpose wa...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2006.09.005

    authors： Stratford PW,Kennedy DM,Woodhouse LJ,Spadoni GF

    更新日期：2007-03-01 00:00:00

  • A meta-analysis of sex differences prevalence, incidence and severity of osteoarthritis.

    abstract：OBJECTIVE:To resolve uncertainty regarding sex differences in osteoarthritis (OA) by performing a meta-analysis of sex differences in OA prevalence, incidence and severity. METHODS:Standard search strategies for population-based studies of OA providing sex-specific data. Random effects meta-analysis to provide pooled ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.joca.2005.04.014

    authors： Srikanth VK,Fryer JL,Zhai G,Winzenberg TM,Hosmer D,Jones G

    更新日期：2005-09-01 00:00:00

  • Distinct expression pattern of early- and late-response genes in normal and osteoarthritic human synovial membranes.

    abstract：:The significance of activating proteins (AP-1), c-fos, c-jun and jun B relative to the AP-1 responsive metallothionein, collagenase and stromelysin gene expression in the pathophysiology of osteoarthritis (OA) was investigated. The 'early' c-fos, c-jun and jun-B mRNAs were ubiquitously expressed in normal and OA human...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(05)80085-8

    authors： Zafarullah M,Martel-Pelletier J,Cloutier JM,Gedamu L,Pelletier JP

    更新日期：1993-07-01 00:00:00

  • The anti-NGF antibody muMab 911 both prevents and reverses pain behaviour and subchondral osteoclast numbers in a rat model of osteoarthritis pain.

    abstract：OBJECTIVE:Nerve growth factor (NGF) has a pivotal role in peripheral hyperalgesia and inflammation; anti-NGF antibodies attenuate pain responses in inflammatory pain models, and in people with osteoarthritis (OA) or low back pain. The aim of this study was to characterise the peripheral mechanisms contributing to the a...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.05.015

    authors： Xu L,Nwosu LN,Burston JJ,Millns PJ,Sagar DR,Mapp PI,Meesawatsom P,Li L,Bennett AJ,Walsh DA,Chapman V

    更新日期：2016-09-01 00:00:00

  • OARSI Clinical Trials Recommendations: An abbreviated regulatory guide to the clinical requirements for development of therapeutics in osteoarthritis.

    abstract：:In this brief abbreviated review of regulatory issues regarding the development of drugs and or devices for the treatment of osteoarthritis (OA), the steps that are expected by both the Food and Drug Administration (FDA) and the European Medicines Agency (EMA) are discussed. ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2015.03.010

    authors： Simon LS

    更新日期：2015-05-01 00:00:00

  • The association between reduced knee joint proprioception and medial meniscal abnormalities using MRI in knee osteoarthritis: results from the Amsterdam osteoarthritis cohort.

    abstract：BACKGROUND:Osteoarthritis (OA) of the knee is characterized by pain and activity limitations. In knee OA, proprioceptive accuracy is reduced and might be associated with pain and activity limitations. Although causes of reduced proprioceptive accuracy are divergent, medial meniscal abnormalities, which are highly preva...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.02.002

    authors： van der Esch M,Knoop J,Hunter DJ,Klein JP,van der Leeden M,Knol DL,Reiding D,Voorneman RE,Gerritsen M,Roorda LD,Lems WF,Dekker J

    更新日期：2013-05-01 00:00:00

  • Relationship between anisotropic diffusion properties and tissue morphology in porcine TMJ disc.

    abstract：OBJECTIVE:To investigate the relationship between anisotropic solute diffusion properties and tissue morphology in porcine temporomandibular joint (TMJ) discs. DESIGN:TMJ discs from eleven pigs aged 6-8 months were divided into five regions: anterior, intermediate, posterior, lateral, and medial. The transport propert...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.01.010

    authors： Shi C,Wright GJ,Ex-Lubeskie CL,Bradshaw AD,Yao H

    更新日期：2013-04-01 00:00:00

  • An MRI derived articular cartilage visualization framework.

    abstract：OBJECTIVE:We present a multi-dimensional framework for the visualization of femoral articular cartilage. The framework comprises methods for visualizing and quantifying changes in cartilage thickness and surface morphology derived from MRI based cartilage segmentation. Adequate visualization of cartilage allows accurat...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.03.009

    authors： Akhtar S,Poh CL,Kitney RI

    更新日期：2007-09-01 00:00:00

  • Fibromodulin-null murine knee joints display increased incidences of osteoarthritis and alterations in tissue biochemistry.

    abstract：OBJECTIVE:To elucidate variations in tissue ultrastructure and incidence of pathology between fibromodulin (FM)-null mice and wild-type (WT) animals. DESIGN:FM-null and WT siblings from different age groups were compared. Serial sections were made through paraffin-embedded whole knees and investigated histologically. ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2002.0527

    authors： Gill MR,Oldberg A,Reinholt FP

    更新日期：2002-10-01 00:00:00

  • Subchondral bone marrow lesions are highly associated with, and predict subchondral bone attrition longitudinally: the MOST study.

    abstract：OBJECTIVE:Subchondral bone attrition (SBA) is defined as flattening or depression of the osseous articular surface. The causes of attrition are unknown, but remodeling processes due to chronic overload that are reflected as bone marrow edema-like lesions (BMLs) on magnetic resonance imaging (MRI) might predispose the s...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.joca.2009.08.018

    authors： Roemer FW,Neogi T,Nevitt MC,Felson DT,Zhu Y,Zhang Y,Lynch JA,Javaid MK,Crema MD,Torner J,Lewis CE,Guermazi A

    更新日期：2010-01-01 00:00:00

  • Role of subchondral bone properties and changes in development of load-induced osteoarthritis in mice.

    abstract：OBJECTIVE:Animal models recapitulating post-traumatic osteoarthritis (OA) suggest that subchondral bone (SCB) properties and remodeling may play major roles in disease initiation and progression. Thus, we investigated the role of SCB properties and its effects on load-induced OA progression by applying a tibial loading...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2017.08.016

    authors： Adebayo OO,Ko FC,Wan PT,Goldring SR,Goldring MB,Wright TM,van der Meulen MCH

    更新日期：2017-12-01 00:00:00

  • Brace treatment for osteoarthritis of the knee: a prospective randomized multi-centre trial.

    abstract：OBJECTIVE:To evaluate the effect of a brace intended to reduce load in patients with medial or lateral compartmental osteoarthritis (OA) and concurrent varus or valgus alignment, respectively. DESIGN:This multi-centre randomized controlled trial (performed 2001-2003) studies the additive effect of a brace intended to ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.joca.2006.02.004

    authors： Brouwer RW,van Raaij TM,Verhaar JA,Coene LN,Bierma-Zeinstra SM

    更新日期：2006-08-01 00:00:00

  • Comparison of mechanical debridement and radiofrequency energy for chondroplasty in an in vivo equine model of partial thickness cartilage injury.

    abstract：OBJECTIVE:The purpose of this study was to develop a long-term model of cartilage injury that could be used to compare the effects of radiofrequency energy (RFE) and mechanical debridement as a treatment. METHODS:Partial thickness fibrillation of patellar cartilage was created in 16 mature ponies. Three months after t...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2006.06.021

    authors： Edwards RB 3rd,Lu Y,Uthamanthil RK,Bogdanske JJ,Muir P,Athanasiou KA,Markel MD

    更新日期：2007-02-01 00:00:00

  • Cartilage loss in radiographically normal knees depends on radiographic status of the contralateral knee - data from the Osteoarthritis Initiative.

    abstract：OBJECTIVE:To test whether radiographically normal knees with contralateral radiographic knee osteoarthritis (OA), but without contralateral trauma history, display greater cartilage thickness loss than knees from subjects with bilaterally radiographically normal knees. METHODS:828 radiographically normal knees (Kellgr...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2018.10.006

    authors： Eckstein F,Maschek S,Roemer FW,Duda GN,Sharma L,Wirth W

    更新日期：2019-02-01 00:00:00

  • Prevalence of cam and pincer-type deformities on hip MRI in an asymptomatic young Swiss female population: a cross-sectional study.

    abstract：OBJECTIVES:Femoroacetabular impingement is proposed to cause early osteoarthritis (OA) in the non-dysplastic hip. We previously reported on the prevalence of femoral deformities in a young asymptomatic male population. The aim of this study was to determine the prevalence of both femoral and acetabular types of impinge...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.01.003

    authors： Leunig M,Jüni P,Werlen S,Limacher A,Nüesch E,Pfirrmann CW,Trelle S,Odermatt A,Hofstetter W,Ganz R,Reichenbach S

    更新日期：2013-04-01 00:00:00

  • Exercise-driven metabolic pathways in healthy cartilage.

    abstract：OBJECTIVE:Exercise is vital for maintaining cartilage integrity in healthy joints. Here we examined the exercise-driven transcriptional regulation of genes in healthy rat articular cartilage to dissect the metabolic pathways responsible for the potential benefits of exercise. METHODS:Transcriptome-wide gene expression...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.02.004

    authors： Blazek AD,Nam J,Gupta R,Pradhan M,Perera P,Weisleder NL,Hewett TE,Chaudhari AM,Lee BS,Leblebicioglu B,Butterfield TA,Agarwal S

    更新日期：2016-07-01 00:00:00

  • Association between concentrations of urinary type II collagen neoepitope (uTIINE) and joint space narrowing in patients with knee osteoarthritis.

    abstract：OBJECTIVE:To examine whether urine concentrations of type II collagen neoepitope (uTIINE) distinguish subjects with progressive radiographic and/or symptomatic knee osteoarthritis (OA) from those with stable disease. METHODS:Subjects were 120 obese middle-aged women with unilateral knee OA who participated in a 30-mon...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joca.2006.04.009

    authors： Hellio Le Graverand MP,Brandt KD,Mazzuca SA,Katz BP,Buck R,Lane KA,Pickering E,Nemirovskiy OV,Sunyer T,Welsch DJ

    更新日期：2006-11-01 00:00:00

  • Bone marrow stimulation induces greater chondrogenesis in trochlear vs condylar cartilage defects in skeletally mature rabbits.

    abstract：OBJECTIVE:The aim of this study was to compare the early repair response of cartilage defects in trochlea (TR) and medial femoral condyle (MFC) at 2-3 weeks after bone marrow stimulation. DESIGN:Bilateral full-thickness cartilage defects were generated in central trochlear groove and MFC of skeletally mature rabbits. ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.04.010

    authors： Chen H,Chevrier A,Hoemann CD,Sun J,Lascau-Coman V,Buschmann MD

    更新日期：2013-07-01 00:00:00