Abstract:
OBJECTIVE:Exercise is vital for maintaining cartilage integrity in healthy joints. Here we examined the exercise-driven transcriptional regulation of genes in healthy rat articular cartilage to dissect the metabolic pathways responsible for the potential benefits of exercise. METHODS:Transcriptome-wide gene expression in the articular cartilage of healthy Sprague-Dawley female rats exercised daily (low intensity treadmill walking) for 2, 5, or 15 days was compared to that of non-exercised rats, using Affymetrix GeneChip arrays. Database for Annotation, Visualization and Integrated Discovery (DAVID) was used for Gene Ontology (GO)-term enrichment and Functional Annotation analysis of differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genome (KEGG) pathway mapper was used to identify the metabolic pathways regulated by exercise. RESULTS:Microarray analysis revealed that exercise-induced 644 DEGs in healthy articular cartilage. The DAVID bioinformatics tool demonstrated high prevalence of functional annotation clusters with greater enrichment scores and GO-terms associated with extracellular matrix (ECM) biosynthesis/remodeling and inflammation/immune response. The KEGG database revealed that exercise regulates 147 metabolic pathways representing molecular interaction networks for Metabolism, Genetic Information Processing, Environmental Information Processing, Cellular Processes, Organismal Systems, and Diseases. These pathways collectively supported the complex regulation of the beneficial effects of exercise on the cartilage. CONCLUSIONS:Overall, the findings highlight that exercise is a robust transcriptional regulator of a wide array of metabolic pathways in healthy cartilage. The major actions of exercise involve ECM biosynthesis/cartilage strengthening and attenuation of inflammatory pathways to provide prophylaxis against onset of arthritic diseases in healthy cartilage.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Blazek AD,Nam J,Gupta R,Pradhan M,Perera P,Weisleder NL,Hewett TE,Chaudhari AM,Lee BS,Leblebicioglu B,Butterfield TA,Agarwal Sdoi
10.1016/j.joca.2016.02.004subject
Has Abstractpub_date
2016-07-01 00:00:00pages
1210-22issue
7eissn
1063-4584issn
1522-9653pii
S1063-4584(16)01025-6journal_volume
24pub_type
杂志文章abstract:OBJECTIVE:The effects of mechanical deformation of intact cartilage tissue on chondrocyte biosynthesis in situ have been well documented, but the mechanotransduction pathways that regulate such phenomena have not been elucidated completely. The goal of this study was to examine the effects of tissue deformation on the ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2004.08.004
更新日期:2004-12-01 00:00:00
abstract:OBJECTIVE:Nerve growth factor (NGF) has a pivotal role in peripheral hyperalgesia and inflammation; anti-NGF antibodies attenuate pain responses in inflammatory pain models, and in people with osteoarthritis (OA) or low back pain. The aim of this study was to characterise the peripheral mechanisms contributing to the a...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2016.05.015
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVE:To date, no approved clinical intervention successfully prevents the progressive degradation of injured articular cartilage that leads to osteoarthritis (OA). Stem/progenitor cell populations within tissues of diarthrodial joint have shown their therapeutic potential in treating OA. However, this potential ha...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2014.07.002
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVE:Animal models recapitulating post-traumatic osteoarthritis (OA) suggest that subchondral bone (SCB) properties and remodeling may play major roles in disease initiation and progression. Thus, we investigated the role of SCB properties and its effects on load-induced OA progression by applying a tibial loading...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2017.08.016
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVE:Cam hip morphology is associated with femoroacetabular impingement (FAI) syndrome and causes hip osteoarthritis (OA). We aimed to assess the prevalence of cam hip morphology in a sample representative of the general population, using a measure with a predefined diagnostic accuracy. DESIGN:Patients aged 16-65...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2018.09.019
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:Conduct a systematic review and use meta-analytic techniques to estimate the proportion of total treatment effect that can be attributable to contextual effects (PCE) in adults receiving nonpharmacological, nonsurgical (NPNS) treatments for knee osteoarthritis (OA). DESIGN:We reviewed the published literatur...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2020.05.007
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:As cartilage loss and bone marrow lesions (BMLs) are associated with knee joint pain and structural worsening, this study assessed whether non-invasive estimates of articular contact stress may longitudinally predict risk for worsening of knee cartilage morphology and BMLs. DESIGN:This was a longitudinal coh...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2012.05.013
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVE:To evaluate whether baseline T1ρ and T2 relaxation times of hip cartilage are associated with magnetic resonance imaging (MRI) based progression of hip osteoarthritis (OA) at 18 months. METHODS:3T MRI studies of the hip were obtained at baseline and 18-month follow-up for 54 subjects without evidence of seve...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2016.03.005
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVE:Characterize the effects of alendronate (ALN) on the repair process of the osteonecrotic femoral head as well as the development of secondary osteoarthritis in the ipsilateral hip in an established experimental model of osteonecrosis. METHODS:Osteonecrosis of the femoral head was induced surgically in 60 adu...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2008.07.013
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:To determine in human articular chondrocytes the activity of Aldehyde dehydrogenase (ALDH), which are reported as stem/progenitor cell marker in various adult tissues and evaluate gene expression of ALDH1A isoforms. DESIGN:ALDH activity was evaluated by flow cytometry with Aldefluor™ assay in cells, isolated...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.11.019
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVES:The generation of transgenic mice expressing green fluorescent proteins (GFPs) has greatly aided our understanding of the development of connective tissues such as bone and cartilage. Perturbation of a biological system such as the temporomandibular joint (TMJ) within its adaptive remodeling capacity is part...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.08.010
更新日期:2016-02-01 00:00:00
abstract::The extracellular matrix of articular cartilage consists mainly of type II collagen and large aggregating proteoglycan (aggrecan). During arthritis and other joint diseases, the proteoglycan (PG) level of cartilage matrix is diminished, leading to impairment of normal joint function. A new method is described for meas...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/s1063-4584(05)80070-6
更新日期:1994-09-01 00:00:00
abstract:OBJECTIVE:To describe the reproducibility and validity of six different measurement techniques for knee subchondral bone mineral density (sBMD). METHODS:A consecutive sample of 50 male and female participants from a population-based longitudinal study had sBMD assessed using dual energy X-ray absorptiometry scans. Ant...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2008.04.012
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVE:Hip labrum pathology has only begun to emerge as a significant source of groin pain in the last decade since the development of hip arthroscopy. Few data are available on the anatomy, histology and function of this structure. Moreover, no metabolic data exist at cellular level. The aim of this study was to ch...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2012.03.023
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVE:Summarize the comparative effectiveness of oral non-steroidal anti-inflammatory drugs (NSAIDs) and opioids in reducing knee osteoarthritis (OA) pain. METHODS:Two reviewers independently screened reports of randomized controlled trials (RCTs), published in English between 1982 and 2015, evaluating oral NSAIDs...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2016.01.135
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:To study the effect of 17β-estradiol (E2) and the superficial zone (SFZ) on cell death and proteoglycan degradation in articular cartilage after a single injurious compression in vitro. METHOD:Cartilage explants from the femoropatellar groove of 2 year old cows with or without the SFZ were cultured serum-fre...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2013.07.007
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:To examine the effects of anterior cruciate ligament (ACL) insufficiency, and subsequent bisphosphonate (BP) antiresorptive therapy, on the bone mineral interface at the enthesis of remaining ligamentous restraints. METHODS:We measured bone mineral geometry (and subsequent adaptation) at the medial collatera...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2005.04.015
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of waiting time on health and quality of life outcomes and costs of medication in total hip replacement (THR) patients in a randomized clinical trial. METHODS:395 THR patients were recruited into the study. When placed on the waiting list, patients were randomized into a short (< or =3...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.joca.2009.03.014
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVE:Aging can cause an increase in the stiffness of hyaline cartilage as a consequence of increased protein crosslinks. By induction of crosslinking, a reduction in the diffusion of solutions into the hyaline cartilage has been observed. However, there is a lack of knowledge about the effects of aging on the biop...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2018.08.007
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:To examine whether urine concentrations of type II collagen neoepitope (uTIINE) distinguish subjects with progressive radiographic and/or symptomatic knee osteoarthritis (OA) from those with stable disease. METHODS:Subjects were 120 obese middle-aged women with unilateral knee OA who participated in a 30-mon...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joca.2006.04.009
更新日期:2006-11-01 00:00:00
abstract:OBJECTIVE:To test the efficacy of phase-sensitive X-ray imaging for intact synovial joints, whereby refraction effects, along with the attenuation of conventional radiography, can be exploited. DESIGN:Intact cadaveric human knee joints were imaged, in the computed tomographic mode, using an analyzer-based X-ray system...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2009.03.005
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVE:During exercise, cartilage recovers interstitial fluid lost during inactivity, which explains how tissue thickness and joint space are maintained over time. This recovery phenomenon is currently explained by a combination of osmotic swelling during intermittent bath exposure and sub-ambient pressurization dur...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2016.09.018
更新日期:2017-01-01 00:00:00
abstract::A major objective of this article is to examine the research implications of recently available genome-wide expression profiles of cartilage from human osteoarthritis (OA) joints. We propose that, when viewed in the light of extensive earlier work, this novel data provides a unique opportunity to reorient the design o...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2015.03.027
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To determine the association of varus thrust during walking to incident and worsening medial tibiofemoral cartilage damage and bone marrow lesions (BMLs) over 2 years in older adults with or at risk for osteoarthritis (OA). METHOD:Subjects from the Multicenter Osteoarthritis Study (MOST) were studied. Varus ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,多中心研究
doi:10.1016/j.joca.2017.01.005
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVE:Micro-computed tomography (microCT) imaging has the potential to allow the three-dimensional (3D) visualization of cartilage morphology. However, cartilage intensity on a microCT image is weak because cartilage does not strongly attenuate X-rays. This work was designed to demonstrate that exposure of cartilag...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2005.08.010
更新日期:2006-03-01 00:00:00
abstract:OBJECTIVE:When chondrocytes prepared from cartilage are expanded in monolayer culture, fibroblast-like cells gradually prevail. Although these prevailing fibroblast-like cells are believed to emerge because of the dedifferentiation of chondrocytes, the definite origin of the prevailing fibroblast-like cells has not bee...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2013.09.004
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVE:Osteoarthritis (OA) is associated with increased articular cartilage hydraulic permeability and decreased maintenance of high interstitial fluid load support (IFLS) during articulation, resulting in increased friction on the cartilage solid matrix. This study assesses frictional response following in situ syn...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2017.03.001
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVE:To update our earlier systematic review which evaluated all published randomized controlled trials (RCTs) evaluating pharmacological and non-pharmacological therapies in patients with hand osteoarthritis (OA). Surgical therapies were not evaluated. METHOD:RCTs published between August 2004 and February 2008 ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2009.04.006
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVE:To assess the effectiveness of short-wave diathermy (SWD) treatment in the management of knee osteoarthritis (KOA) and to assess whether the effects are related to the induction of a thermal effect. METHODS:A systematic literature search was conducted in PubMed, CINAHL, PEDro, EMBASE, SPORTdiscus and Scholar...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.joca.2012.05.005
更新日期:2012-09-01 00:00:00
abstract:OBJECTIVE:Recent developments in optical clearing and microscopy technology have enabled the imaging of intact tissues at the millimeter scale to characterize cells via fluorescence labeling. While these techniques have facilitated the three-dimensional (3D) cellular characterization within brain and heart, study of de...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2014.11.021
更新日期:2015-03-01 00:00:00