当前位置： SCI文献检索 > OSTEOARTHRITIS AND CARTILAGE期刊下所有文献 > Nondestructive assessment of sGAG content and distribution in normal and degraded rat articular cartilage via EPIC-microCT.

Nondestructive assessment of sGAG content and distribution in normal and degraded rat articular cartilage via EPIC-microCT.

Abstract：

OBJECTIVE:The objective of this study was to evaluate the feasibility of quantifying the Equilibrium Partitioning of an Ionic Contrast agent via Microcomputed Tomography (EPIC-microCT) to nondestructively assess sulfated glycosaminoglycan (sGAG) content and distribution in rat articular cartilage ex vivo, and in doing so to establish a paradigm for extension of this technique to other small animal models. DESIGN:After determination of an appropriate incubation time for the anionic contrast agent, EPIC-microCT was used to examine age-related differences in cartilage sGAG content between 4-, 8-, and 16-week old (n=5 each) male Wistar rats and to evaluate sGAG depletion in the right femora of each age group after 60 min of digestion with chondroitinase ABC. The EPIC-microCT measurements were validated by histological safranin-O staining, and reproducibility was evaluated by triplicate scans of six femora. RESULTS:Cartilage attenuation gradually increased with cumulative digestion time and reached a plateau at approximately 60 min with a 16.0% temporal increase (P<0.01). Average femoral articular cartilage attenuation increased by 14.2% from 4- to 8-weeks of age (P<0.01) and further increased by 2.5% from 8 to 16 weeks (P<0.05). After 60 min of digestion, femoral articular cartilage attenuations increased by 15-17% in each age group (P<0.01). Correspondingly, sGAG optical density decreased with age and digestion, and showed a linear correlation (r=-0.88, slope=-1.26, P<0.01, n=30) with EPIC-microCT cartilage attenuation. High reproducibility was indicated by a low coefficient of variation (1.5%) in cartilage attenuation. CONCLUSIONS:EPIC-microCT imaging provides high spatial resolution and sensitivity to assess sGAG content and three-dimensional distribution in rat femoral articular cartilage.

journal_name

Osteoarthritis Cartilage

journal_title

Osteoarthritis and cartilage

authors

Xie L,Lin AS,Guldberg RE,Levenston ME

doi

10.1016/j.joca.2009.07.014

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

65-72

issue

1

eissn

1063-4584

issn

1522-9653

pii

S1063-4584(09)00203-9

journal_volume

18

pub_type

杂志文章

相关文献

OSTEOARTHRITIS AND CARTILAGE文献大全
  • Osteoarthritis-related difficulty walking and risk for diabetes complications.

    abstract：OBJECTIVES:To examine the effect of Osteoarthritis (OA)-related difficulty walking on risk for diabetes complications in persons with diabetes and OA. DESIGN:A population cohort aged 55+ years with symptomatic hip and knee OA was recruited 1996-98 and followed through provincial administrative data to 2015 (n = 2,225)...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.08.003

    authors： Hawker GA,Croxford R,Bierman AS,Harvey P,Ravi B,Kendzerska T,Stanaitis I,King LK,Lipscombe L

    更新日期：2017-01-01 00:00:00

  • Serum levels of type IIA procollagen amino terminal propeptide (PIIANP) are decreased in patients with knee osteoarthritis and rheumatoid arthritis.

    abstract：OBJECTIVE:The aim of this study was to develop a specific immunoassay for PIIANP and measure its serum concentration in healthy controls and in patients with osteoarthritis (OA) and rheumatoid arthritis (RA). In addition, we investigated circulating forms recognized by antiserum IIA in pools of serum from healthy adult...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2004.02.004

    authors： Rousseau JC,Zhu Y,Miossec P,Vignon E,Sandell LJ,Garnero P,Delmas PD

    更新日期：2004-06-01 00:00:00

  • Poor replicability of recommended exercise interventions for knee osteoarthritis: a descriptive analysis of evidence informing current guidelines and recommendations.

    abstract：OBJECTIVE:To examine the reporting completeness of exercise-based interventions for knee osteoarthritis (OA) in studies that form the basis of current clinical guidelines, and examine if the clinical benefit (pain and disability) from exercise is associated with the intervention reporting completeness. DESIGN:Review o...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.joca.2018.06.018

    authors： Bartholdy C,Nielsen SM,Warming S,Hunter DJ,Christensen R,Henriksen M

    更新日期：2019-01-01 00:00:00

  • Dicam promotes proliferation and maturation of chondrocyte through Indian hedgehog signaling in primary cilia.

    abstract：OBJECTIVES:Primary cilium is required for mechano-biological signal transduction in chondrocytes, and its interaction with extracellular matrix is critical for cartilage homeostasis. However, the role of cilia-associated proteins that affect the function of cilia remains to be elucidated. Here, we show that Dicam has a...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2018.04.008

    authors： Han S,Park HR,Lee EJ,Jang JA,Han MS,Kim GW,Jeong JH,Choi JY,Beier F,Jung YK

    更新日期：2018-07-01 00:00:00

  • The influence of mechanical compression on the induction of osteoarthritis-related biomarkers in articular cartilage explants.

    abstract：OBJECTIVE:Macromolecules of the articular cartilage extracellular matrix released into synovial fluid, blood, or urine can serve as potentially useful biomarkers of the severity of osteoarthritis (OA). Biomechanical factors play an important role in OA pathogenesis, yet their influence on biomarker production is not we...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2005.07.003

    authors： Piscoya JL,Fermor B,Kraus VB,Stabler TV,Guilak F

    更新日期：2005-12-01 00:00:00

  • Automated segmentation and analysis of normal and osteoarthritic knee menisci from magnetic resonance images--data from the Osteoarthritis Initiative.

    abstract：OBJECTIVE:To validate an automatic scheme for the segmentation and quantitative analysis of the medial meniscus (MM) and lateral meniscus (LM) in magnetic resonance (MR) images of the knee. METHOD:We analysed sagittal water-excited double-echo steady-state MR images of the knee from a subset of the Osteoarthritis Init...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2014.06.029

    authors： Paproki A,Engstrom C,Chandra SS,Neubert A,Fripp J,Crozier S

    更新日期：2014-09-01 00:00:00

  • Comparative evaluation of three semi-quantitative radiographic grading techniques for hip osteoarthritis in terms of validity and reproducibility in 1404 radiographs: report of the OARSI-OMERACT Task Force.

    abstract：OBJECTIVE:The objective of this work was to compare the measurement properties of three categorical X-ray scoring methods for hip osteoarthritis (OA). METHODS:In data obtained from trials and cohorts, radiographs were evaluated using the Kellgren and Lawrence (KL) grading system, the Osteoarthritis Research Society In...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2008.06.009

    authors： Gossec L,Jordan JM,Lam MA,Fang F,Renner JB,Davis A,Hawker GA,Dougados M,Maillefert JF,OARSI-OMERACT task force \total articular replacement as outcome measure in OA\.

    更新日期：2009-02-01 00:00:00

  • T1ρ and T2 relaxation times are associated with progression of hip osteoarthritis.

    abstract：OBJECTIVE:To evaluate whether baseline T1ρ and T2 relaxation times of hip cartilage are associated with magnetic resonance imaging (MRI) based progression of hip osteoarthritis (OA) at 18 months. METHODS:3T MRI studies of the hip were obtained at baseline and 18-month follow-up for 54 subjects without evidence of seve...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.03.005

    authors： Gallo MC,Wyatt C,Pedoia V,Kumar D,Lee S,Nardo L,Link TM,Souza RB,Majumdar S

    更新日期：2016-08-01 00:00:00

  • Magnetic resonance imaging (MRI) of articular cartilage in knee osteoarthritis (OA): morphological assessment.

    abstract：OBJECTIVE:Magnetic resonance imaging (MRI) is a three-dimensional imaging technique with unparalleled ability to evaluate articular cartilage. This report reviews the current status of morphological assessment of cartilage with quantitative MRI (qMRI), and its relevance for identifying disease status, and monitoring pr...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2006.02.026

    authors： Eckstein F,Cicuttini F,Raynauld JP,Waterton JC,Peterfy C

    更新日期：2006-01-01 00:00:00

  • Macroscopic cartilage repair scoring of defect fill, integration and total points correlate with corresponding items in histological scoring systems - a study in adult sheep.

    abstract：OBJECTIVE:To correlate osteochondral repair assessed by validated macroscopic scoring systems with established semiquantitative histological analyses in an ovine model and to test the hypothesis that important macroscopic individual categories correlate with their corresponding histological counterparts. METHODS:In th...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.10.014

    authors： Goebel L,Orth P,Cucchiarini M,Pape D,Madry H

    更新日期：2017-04-01 00:00:00

  • Effect of glucosamine sulphate on joint space narrowing, pain and function in patients with hip osteoarthritis; subgroup analyses of a randomized controlled trial.

    abstract：OBJECTIVE:Recently we reported that glucosamine sulphate (GS) did not have an effect on the symptoms and progression of primary care patients with hip osteoarthritis (OA). The aim of this present study was to investigate whether there are subgroups of patients with hip OA for whom GS might be an effective therapy. MET...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joca.2008.05.022

    authors： Rozendaal RM,Uitterlinden EJ,van Osch GJ,Garling EH,Willemsen SP,Ginai AZ,Verhaar JA,Weinans H,Koes BW,Bierma-Zeinstra SM

    更新日期：2009-04-01 00:00:00

  • An MRI derived articular cartilage visualization framework.

    abstract：OBJECTIVE:We present a multi-dimensional framework for the visualization of femoral articular cartilage. The framework comprises methods for visualizing and quantifying changes in cartilage thickness and surface morphology derived from MRI based cartilage segmentation. Adequate visualization of cartilage allows accurat...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.03.009

    authors： Akhtar S,Poh CL,Kitney RI

    更新日期：2007-09-01 00:00:00

  • Coexisting lateral tibiofemoral osteoarthritis is associated with worse knee pain in patients with mild medial osteoarthritis.

    abstract：OBJECTIVE:To examine the clinical impact of coexisting lateral osteoarthritis (OA) in knees with mild medial OA. DESIGN:In patients with Kellgren/Lawrence (K/L) grade 2 OA in the medial compartment (n = 100; age: 56-89 years; 80.0% female), anteroposterior knee radiography was used to assess the presence of lateral OA...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2017.02.801

    authors： Iijima H,Aoyama T,Nishitani K,Ito H,Fukutani N,Isho T,Kaneda E,Kuroki H,Matsuda S

    更新日期：2017-08-01 00:00:00

  • Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage.

    abstract：OBJECTIVE:To date, no approved clinical intervention successfully prevents the progressive degradation of injured articular cartilage that leads to osteoarthritis (OA). Stem/progenitor cell populations within tissues of diarthrodial joint have shown their therapeutic potential in treating OA. However, this potential ha...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2014.07.002

    authors： Yu Y,Zheng H,Buckwalter JA,Martin JA

    更新日期：2014-09-01 00:00:00

  • Time-lapse observation of the dedifferentiation process in mouse chondrocytes using chondrocyte-specific reporters.

    abstract：OBJECTIVE:When chondrocytes prepared from cartilage are expanded in monolayer culture, fibroblast-like cells gradually prevail. Although these prevailing fibroblast-like cells are believed to emerge because of the dedifferentiation of chondrocytes, the definite origin of the prevailing fibroblast-like cells has not bee...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.09.004

    authors： Minegishi Y,Hosokawa K,Tsumaki N

    更新日期：2013-12-01 00:00:00

  • Systematic review and meta-analysis of the reliability and discriminative validity of cartilage compositional MRI in knee osteoarthritis.

    abstract：OBJECTIVE:To assess reliability and discriminative validity of cartilage compositional magnetic resonance imaging (MRI) in knee osteoarthritis (OA). DESIGN:The study was carried out per PRISMA recommendations. We searched MEDLINE and EMBASE (1974 - present) for eligible studies. We performed qualitative synthesis of r...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.joca.2017.11.018

    authors： MacKay JW,Low SBL,Smith TO,Toms AP,McCaskie AW,Gilbert FJ

    更新日期：2018-09-01 00:00:00

  • Autophagy is activated in compression-induced cell degeneration and is mediated by reactive oxygen species in nucleus pulposus cells exposed to compression.

    abstract：OBJECTIVE:To determine whether autophagy contributes to the pathogenesis of degenerative disc disease (DDD) or retards the intervertebral disc (IVD) degeneration, and investigate the possible relationship between compression-induced autophagy and intracellular reactive oxygen species (ROS) in nucleus pulposus (NP) cell...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.10.002

    authors： Ma KG,Shao ZW,Yang SH,Wang J,Wang BC,Xiong LM,Wu Q,Chen SF

    更新日期：2013-12-01 00:00:00

  • Diffusion of Gd-DTPA²⁻ into articular cartilage.

    abstract：OBJECTIVES:The delayed Gadolinium-Enhanced MRI of Cartilage (dGEMRIC) technique is a method proposed for non-invasive measurement of cartilage glycosaminoglycan (GAG) content. In this method, gadopentetate (Gd-DTPA²⁻) is assumed to distribute in cartilage in inverse relation to the GAG distribution, thus allowing quant...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2011.11.016

    authors： Salo EN,Nissi MJ,Kulmala KA,Tiitu V,Töyräs J,Nieminen MT

    更新日期：2012-02-01 00:00:00

  • Non-invasive mouse models of post-traumatic osteoarthritis.

    abstract：:Animal models of osteoarthritis (OA) are essential tools for investigating the development of the disease on a more rapid timeline than human OA. Mice are particularly useful due to the plethora of genetically modified or inbred mouse strains available. The majority of available mouse models of OA use a joint injury o...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2015.05.009

    authors： Christiansen BA,Guilak F,Lockwood KA,Olson SA,Pitsillides AA,Sandell LJ,Silva MJ,van der Meulen MC,Haudenschild DR

    更新日期：2015-10-01 00:00:00

  • Standardization of nutrient media for isolated human articular chondrocytes in gelified agarose suspension culture.

    abstract：:Human articular cartilage cells were cultured in 1.5% agarose in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal calf serum or in serum-free DMEM with 0.15% bovine serum albumin. 35S-aggrecan synthesis in serum-free DMEM was between 20% and 30% of the value observed in DMEM supplemented with 10% fetal calf se...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(05)80016-0

    authors： Verbruggen G,Malfait AM,Dewulf M,Broddelez C,Veys EM

    更新日期：1995-12-01 00:00:00

  • Symptomatic efficacy of avocado-soybean unsaponifiables (ASU) in osteoarthritis (OA) patients: a meta-analysis of randomized controlled trials.

    abstract：OBJECTIVE:To evaluate the efficacy of preparations with avocado-soybean unsaponifiables (ASUs) in osteoarthritis (OA) patients using meta-analysis on randomized controlled trials (RCTs). METHOD:RCTs from systematic searches were included if they explicitly stated that hip and/or knee OA patients were randomized to eit...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.joca.2007.10.003

    authors： Christensen R,Bartels EM,Astrup A,Bliddal H

    更新日期：2008-04-01 00:00:00

  • Change in serum measurements of cartilage oligomeric matrix protein and association with the development and worsening of radiographic hip osteoarthritis.

    abstract：OBJECTIVE:To determine the association between changes in serum levels of cartilage oligomeric matrix protein (COMP) and serum N-telopeptide crosslinks (NTX) over a 6-year interval with the development and progression of radiographically apparent hip osteoarthritis (RHOA) in a community sample of elderly women over 8.3...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.joca.2007.09.008

    authors： Chaganti RK,Kelman A,Lui L,Yao W,Javaid MK,Bauer D,Nevitt M,Lane NE,Study Of Osteoporotic Fractures Research Group Sof.

    更新日期：2008-05-01 00:00:00

  • Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA).

    abstract：OBJECTIVES:To address the hypothesis that different types of established osteoarthritis (OA) pain behaviours have associations with different aspects of articular pathology, we investigated the relationship between structural knee joint pathology and pain behaviour following injection of a low vs a high dose of monosod...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.06.012

    authors： Nwosu LN,Mapp PI,Chapman V,Walsh DA

    更新日期：2016-11-01 00:00:00

  • Test-retest reliability and responsiveness of centre of pressure measurements in patients with hip osteoarthritis.

    abstract：OBJECTIVE:The aim of this study was to determine a set of measures for the evaluation of balance in patients suffering from hip osteoarthritis (OA) that were both reliable and responsive to change. DESIGN:Three groups of subjects; Healthy, hip OA patients without surgery, and hip OA with surgery (pre and post-surgery)...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2015.03.029

    authors： Laroche D,Kubicki A,Stapley PJ,Gremeaux V,Mazalovic K,Maillefert JF,Ornetti P

    更新日期：2015-08-01 00:00:00

  • Validity of self-report measures of pain and disability for persons who have undergone arthroplasty for osteoarthritis of the carpometacarpal joint of the hand.

    abstract：OBJECTIVE:To establish the validity of three self-report scales used to measure function following arthroplasty for osteoarthritis (OA) of the carpometacarpal joint. METHOD:Persons with OA of the carpometacarpal joint (n=122) were assessed on one occasion 9-117 months following tendon interposition arthroplasty. They ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2006.10.018

    authors： MacDermid JC,Wessel J,Humphrey R,Ross D,Roth JH

    更新日期：2007-05-01 00:00:00

  • Safety, tolerability, and pharmacodynamics of an anti-interleukin-1α/β dual variable domain immunoglobulin in patients with osteoarthritis of the knee: a randomized phase 1 study.

    abstract：OBJECTIVE:To investigate the safety, tolerability, pharmacokinetics, and pharmacodynamics of ABT-981, a human dual variable domain immunoglobulin simultaneously targeting interleukin (IL)-1α and IL-1β, in patients with knee osteoarthritis (OA). METHOD:This was a randomized, double-blind, placebo-controlled, single-cen...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joca.2017.09.007

    authors： Wang SX,Abramson SB,Attur M,Karsdal MA,Preston RA,Lozada CJ,Kosloski MP,Hong F,Jiang P,Saltarelli MJ,Hendrickson BA,Medema JK

    更新日期：2017-12-01 00:00:00

  • Depth-dependent compressive properties of normal aged human femoral head articular cartilage: relationship to fixed charge density.

    abstract：OBJECTIVES:Determine the depth-varying confined and osmotic compression moduli of normal human articular cartilage from the femoral head, and test whether these moduli are dependent on fixed charge density. METHODS AND RESULTS:Using an automated instrument to allow epifluorescence microscopy analysis during confined c...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2001.0424

    authors： Chen SS,Falcovitz YH,Schneiderman R,Maroudas A,Sah RL

    更新日期：2001-08-01 00:00:00

  • Efficacy of a combination of FCHG49 glucosamine hydrochloride, TRH122 low molecular weight sodium chondroitin sulfate and manganese ascorbate in the management of knee osteoarthritis.

    abstract：OBJECTIVES:The objective of this study was to evaluate the oral combination of glucosamine HCl, sodium chondroitin sulfate and manganese ascorbate for the treatment of osteoarthritis (OA) of the knee. DESIGN:A randomized placebo-controlled study design was implemented. We recruited 93 patients with OA of the knee from...

    journal_title：Osteoarthritis and cartilage

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1053/joca.1999.0308

    authors： Das A Jr,Hammad TA

    更新日期：2000-09-01 00:00:00

  • The effect of waiting time on health and quality of life outcomes and costs of medication in hip replacement patients: a randomized clinical trial.

    abstract：OBJECTIVE:To evaluate the effect of waiting time on health and quality of life outcomes and costs of medication in total hip replacement (THR) patients in a randomized clinical trial. METHODS:395 THR patients were recruited into the study. When placed on the waiting list, patients were randomized into a short (< or =3...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.joca.2009.03.014

    authors： Tuominen U,Sintonen H,Hirvonen J,Seitsalo S,Paavolainen P,Lehto M,Hietaniemi K,Blom M

    更新日期：2009-09-01 00:00:00

  • Expression of microRNAs during chondrogenesis of human adipose-derived stem cells.

    abstract：OBJECTIVES:MicroRNAs (miRNAs) play an important role in the regulation of chondrogenesis of mesenchymal stem cells, but their expression still remains unknown in human adipose-derived stem cells (hADSCs). In this study the miRNA expression profile during chondrogenic differentiation of hADSC and the potential mechanism...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2012.08.024

    authors： Zhang Z,Kang Y,Zhang Z,Zhang H,Duan X,Liu J,Li X,Liao W

    更新日期：2012-12-01 00:00:00