Distribution and expression of non-muscle myosin light chain kinase in rabbit livers.

Abstract:

AIM:To study the distribution and expression of non-muscle myosin light chain kinase (nmMLCK) in rabbit livers. METHODS:Human nmMLCK N-terminal cDNA was amplified by polymerase chain reaction (PCR) and was inserted into pBKcmv to construct expression vectors. The recombinant plasmid was transformed into XL1-blue. Expression protein was induced by IPTG and then purified by SDS-PAGE and electroelution, which was used to prepare the polycolonal antibody to detect the distribution and expression of nmMLCK in rabbit livers with immunofluorescene techniques. RESULTS:The polyclonal antibody was prepared, by which nmMLCK expression was detected and distributed mainly in peripheral hepatocytes. CONCLUSION:nmMLCK can express in hepatocytes peripherally, and may play certain roles in the regulation of hepatic functions.

journal_name

World J Gastroenterol

authors

Zhu HQ,Wang Y,Hu RL,Ren B,Zhou Q,Jiang ZK,Gui SY

doi

10.3748/wjg.v9.i12.2715

keywords:

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

2715-9

issue

12

eissn

1007-9327

issn

2219-2840

journal_volume

9

pub_type

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