Abstract:
AIM:To study the distribution and expression of non-muscle myosin light chain kinase (nmMLCK) in rabbit livers. METHODS:Human nmMLCK N-terminal cDNA was amplified by polymerase chain reaction (PCR) and was inserted into pBKcmv to construct expression vectors. The recombinant plasmid was transformed into XL1-blue. Expression protein was induced by IPTG and then purified by SDS-PAGE and electroelution, which was used to prepare the polycolonal antibody to detect the distribution and expression of nmMLCK in rabbit livers with immunofluorescene techniques. RESULTS:The polyclonal antibody was prepared, by which nmMLCK expression was detected and distributed mainly in peripheral hepatocytes. CONCLUSION:nmMLCK can express in hepatocytes peripherally, and may play certain roles in the regulation of hepatic functions.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Zhu HQ,Wang Y,Hu RL,Ren B,Zhou Q,Jiang ZK,Gui SYdoi
10.3748/wjg.v9.i12.2715keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
2715-9issue
12eissn
1007-9327issn
2219-2840journal_volume
9pub_type
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