Translocation of a folded protein across the outer membrane in Escherichia coli.

abstract：:T cells transferred into severe lymphopenic hosts undergo rapid proliferation known as "endogenous proliferation" that are distinct from conventional homeostatic proliferation. Unlike homeostatic proliferation, cytokines, such as IL-7 are dispensable, yet TCR:MHC interaction is essential for this process to occur. How...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Do JS,Min B

更新日期：2009-12-01 00:00:00

abstract：:All vertebrate cells have been shown to contain a gene, sarc, that has some homology with the transforming gene of Rous sarcoma virus, src. We have compared the polypeptide products of the sarc gene, p60(sarc), of human, mouse, and chicken cells with the polymorphic polypeptide product of the src gene, p60(src), of se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sefton BM,Hunter T,Beemon K

更新日期：1980-04-01 00:00:00

abstract：:An in-depth knowledge of the interaction of water with amorphous silica is critical to fundamental studies of interfacial hydration water, as well as to industrial processes such as catalysis, nanofabrication, and chromatography. Silica has a tunable surface comprising hydrophilic silanol groups and moderately hydroph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schrader AM,Monroe JI,Sheil R,Dobbs HA,Keller TJ,Li Y,Jain S,Shell MS,Israelachvili JN,Han S

更新日期：2018-03-20 00:00:00

abstract：:The expression of the adenovirus (Ad) early coding region 1a (E1a) is required for virus-induced cell transformation and for the activation of other viral early genes and some cellular genes. Two overlapping early mRNAs of 13S and 12S that are transcribed from this region code for a 289-amino acid protein and a 243-am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hurwitz DR,Chinnadurai G

更新日期：1985-01-01 00:00:00

abstract：:Tumor necrosis factor alpha (TNF-alpha) stimulated the phosphorylation of a 28-kDa protein (p28) in the ME-180 line of human cervical carcinoma cells. The effect of TNF-alpha on the phosphorylation state of p28 was rapid (4-fold increase within 15 min) and persistent, remaining above the basal level for at least 2 hr....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marino MW,Pfeffer LM,Guidon PT Jr,Donner DB

更新日期：1989-11-01 00:00:00

abstract：:Social behavior is often shaped by the rich storehouse of biographical information that we hold for other people. In our daily life, we rapidly and flexibly retrieve a host of biographical details about individuals in our social network, which often guide our decisions as we navigate complex social interactions. Even ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Collins JA,Koski J,Nugiel T,Metoki A,Olson IR

更新日期：2017-04-18 00:00:00

abstract：:T cells that have been immunized to express optimal levels of contact hypersensitivity upon adoptive transfer to normal animals can be inhibited from doing so by incubating them with an antigen-specific T suppressor factor. This factor is composed of at least two subunits which come from cells expressing different Ly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ptak W,Rosenstein RW,Gershon RK

更新日期：1982-04-01 00:00:00

abstract：:Brief learning experience (classical conditioning) induces frequency-specific receptive-field (RF) plasticity in the auditory cortex, characterized as increased response to the frequency of the conditioned stimulus and decreased responses to most other frequencies, including the pretraining best frequency. This experi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinberger NM,Javid R,Lepan B

更新日期：1993-03-15 00:00:00

abstract：:A renin promoter-large tumor antigen (T antigen) fusion gene was constructed to provide a reporter function for renin expression in transgenic mice. These transgenic mice gave rise to tumors in subcutaneous soft tissue, which was attributed to transgene expression at this site. An immunohistochemical analysis of trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sigmund CD,Jones CA,Mullins JJ,Kim U,Gross KW

更新日期：1990-10-01 00:00:00

abstract：:Peroxisome proliferators (PP) induce a highly predictable pleiotropic response in rat and mouse liver that is characterized by hepatomegaly, increase in peroxisome number in hepatocytes, and induction of certain peroxisomal enzymes. The PP-binding protein (PPbP) was purified from rat liver cytosol by a two-step proced...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lalwani ND,Alvares K,Reddy MK,Reddy MN,Parikh I,Reddy JK

更新日期：1987-08-01 00:00:00

abstract：:Precise regulation of the evolutionarily conserved hypoxia-inducible transcription factor (HIF) ensures proper adaptation to variations in oxygen availability throughout development and into adulthood. Oxygen-dependent regulation of HIF stability and activity are mediated by hydroxylation of conserved proline and aspa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dann CE 3rd,Bruick RK,Deisenhofer J

更新日期：2002-11-26 00:00:00

abstract：:Escherichia coli fMet-tRNAfMet alters the pattern of promoter selection of E. coli RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6), affecting RNA synthesis from the rRNA, suIII+tRNA, and lac promoters in different ways. The in vitro synthesis of the stable RNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Debenham PG,Pongs O,Travers AA

更新日期：1980-02-01 00:00:00

abstract：:Thiazides and related diuretics inhibit NaCl reabsorption in the distal tubule through an unknown mechanism. We report here that [3H]metolazone, a diuretic with a thiazide-like mechanism of action, labels a site in rat kidney membranes that has characteristics of the thiazide-sensitive ion transporter. [3H]Metolazone ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beaumont K,Vaughn DA,Fanestil DD

更新日期：1988-04-01 00:00:00

abstract：:We have studied ten phenotypically similar patients with complete androgen insensitivity. All of the patients tested had significantly elevated serum luteinizing hormone and plasma androgens within or above the normal adult male range. On the basis of specific dihydrotestosterone binding by skin fibroblasts, we identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amrhein JA,Meyer WJ 3rd,Jones HW Jr,Migeon CJ

更新日期：1976-03-01 00:00:00

abstract：:Thebaine, an intermediate of morphine biosynthesis in the poppy plant, Papaver somniferum, was transformed to oripavine, codeine, and morphine by rat liver, kidney, and brain microsomes in the presence of an NADPH-generating system. The formation of morphine, codeine, and oripavine was identified by a specific RIA, HP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kodaira H,Spector S

更新日期：1988-02-01 00:00:00

abstract：:Activation of JAK2 by chromosomal translocation or point mutation is a recurrent event in hematopoietic malignancies, including acute leukemias and myeloproliferative disorders. Although the effects of activated JAK2 signaling have been examined in cell lines and murine models, the functional consequences of deregulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kennedy JA,Barabé F,Patterson BJ,Bayani J,Squire JA,Barber DL,Dick JE

更新日期：2006-11-07 00:00:00

abstract：:Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaika NV,Gebregiworgis T,Lewallen ME,Purohit V,Radhakrishnan P,Liu X,Zhang B,Mehla K,Brown RB,Caffrey T,Yu F,Johnson KR,Powers R,Hollingsworth MA,Singh PK

更新日期：2012-08-21 00:00:00

abstract：:Mitogen-activated protein (MAP) kinase kinase is an enzyme that activates the growth factor-regulated MAP kinase in vitro by a mechanism that involves direct phosphorylation of MAP kinase on tyrosine and threonine residues. MAP kinase kinase is stimulated by growth factor treatment of cells and has been shown to be in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahn NG,Campbell JS,Seger R,Jensen AL,Graves LM,Krebs EG

更新日期：1993-06-01 00:00:00

abstract：:It is believed that neural representations of recent experiences become reactivated during sleep, and that this process serves to stabilize associated memories in long-term memory. Here, we initiated this reactivation process for specific memories during slow-wave sleep. Participants studied 50 object-location associa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Dongen EV,Takashima A,Barth M,Zapp J,Schad LR,Paller KA,Fernández G

更新日期：2012-06-26 00:00:00

abstract：:Starting with assignments of proton nuclear magnetic resonance previously made for oxytocin in deuterated dimethylsulfoxide at 220 MHz, we have assigned resonances for the mammalian antidiuretic hormone, lysine vasopressin. The results demonstrate that spectral assignments of neurohypophyseal hormones and their congen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walter R,Glickson JD,Schwartz IL,Havran RT,Meienhofer J,Urry DW

更新日期：1972-07-01 00:00:00

abstract：:Whether and how human populations exposed to the agricultural revolution are still affected by Darwinian selection remains controversial among social scientists, biologists, and the general public. Although methods of studying selection in natural populations are well established, our understanding of selection in hum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Courtiol A,Pettay JE,Jokela M,Rotkirch A,Lummaa V

更新日期：2012-05-22 00:00:00

abstract：:The genome of a thermophilic fungus, Thermomyces lanuginosus, contains a tandemly arranged cluster of sequences that are approximately equal to 50% homologous with the cytoplasmic 5S RNA and that are selectable by hybridization techniques. Unlike typical pseudogenes, these sequences are not truncated; rather, they bea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong WM,Abrahamson JL,Nazar RN

更新日期：1984-03-01 00:00:00

abstract：:Adhesive P pili of uropathogenic Escherichia coli were not assembled by a strain that lacks the periplasmic disulfide isomerase DsbA. This defect was mostly attributed to the immunoglobulin-like pilus chaperone PapD, which possesses an unusual intrasheet disulfide bond between the last two beta-strands of its CD4-like...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacob-Dubuisson F,Pinkner J,Xu Z,Striker R,Padmanhaban A,Hultgren SJ

更新日期：1994-11-22 00:00:00

abstract：:For over 40 y the dominant theory of stomatal behavior has been that plants should open stomates until the carbon gained by an infinitesimal additional opening balances the additional water lost times a water price that is constant at least over short periods. This theory has persisted because of its remarkable succes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolf A,Anderegg WR,Pacala SW

更新日期：2016-11-15 00:00:00

abstract：:Although mathematical models and laboratory experiments have shown that species interactions can generate chaos, field evidence of chaos in natural ecosystems is rare. We report on a pristine rocky intertidal community located in one of the world's oldest marine reserves that has displayed a complex cyclic succession ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benincà E,Ballantine B,Ellner SP,Huisman J

更新日期：2015-05-19 00:00:00

abstract：:Local inhomogeneities in lipid composition play a crucial role in regulation of signal transduction and membrane traffic. Nevertheless, most evidence for microdomains in cells remains indirect, and the nature of membrane inhomogeneities has been difficult to characterize. We used lipid analogs and lipid-anchored prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hao M,Mukherjee S,Maxfield FR

更新日期：2001-11-06 00:00:00

abstract：:Acidification of the extracellular milieu of malignant tumors is reported to increase the invasive behavior of cancer cells. In normal tissues, production of acid is catalyzed by carbonic anhydrases (CAs), some of which are known to be overexpressed in certain cancers. To investigate the functional role of CA activity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parkkila S,Rajaniemi H,Parkkila AK,Kivela J,Waheed A,Pastorekova S,Pastorek J,Sly WS

更新日期：2000-02-29 00:00:00

abstract：:One of the advantages of the production of monoclonal antibodies by tissue culture methods is that they can be internally labeled by using appropriate radioactive amino acid in the culture fluid. Thus, radioactive immunological probes of high specific activity can be prepared. Here we report applications of these inte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cuello AC,Priestley JV,Milstein C

更新日期：1982-01-01 00:00:00

abstract：:Meet Jonathan and Elizabeth. One person is a doctor and the other is a nurse. Who is the doctor? When nothing else is known, the base rate principle favors Jonathan to be the doctor and the fairness principle favors both individuals equally. However, when individuating facts reveal who is actually the doctor, base rat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cao J,Banaji MR

更新日期：2016-07-05 00:00:00

abstract：:Inadequate target exposure is a major cause of high attrition in drug discovery. Here, we show that a label-free method for quantifying the intracellular bioavailability (Fic) of drug molecules predicts drug access to intracellular targets and hence, pharmacological effect. We determined Fic in multiple cellular assay...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mateus A,Gordon LJ,Wayne GJ,Almqvist H,Axelsson H,Seashore-Ludlow B,Treyer A,Matsson P,Lundbäck T,West A,Hann MM,Artursson P

更新日期：2017-07-25 00:00:00