Abstract：
:The regional, cellular, and subcellular localization of phosphorylated p38 MAPK (pp38) was examined by immunocytochemistry, immuofluorescent multiple labeling, and immunoblotting of extracts as well as immunoprecipitates of human postmortem tissue from control and Alzheimer's disease (AD) cases at different Braak stages. "Early AD" cases (Braak stages IV-V) and a subset of Braak stage VI cases have high levels of pp38 immunoreactivity, with the most dense immunoreactivity located in CA2 and subiculum followed by CA1 in the hippocampus. On the contrary, very little pp38 was detected in age-matched controls (Braak stages 0-II). More importantly, as revealed by various multiple labeling experiments, pp38 immunoreactivity is mainly located in neurons bearing early neurofibrillary pathology, but not in typically fibrillar tangles that are densely stained by thioflavin-S. Most pp38-positive neurons only contain a small amount of phospho-tau. Additionally, pp38 immunoreactivity was not associated with senile plaques. At the subcellular level, pp38-immunoreactive granules are usually larger than the granules stained with the lysosomal marker cathepsin D. Immunoblotting with different extraction buffers and immunoprecipitation indicate that pp38 does not or only loosely binds to phospho-tau. Taken together, this study demonstrates that p38 MAPK is activated at early stages of neurofibrillary degeneration in AD hippocampus. The p38 activation may also be linked to neurodegeneration through mechanisms other than neurofibrillary tangle formation.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Sun A,Liu M,Nguyen XV,Bing Gdoi
10.1016/s0014-4886(03)00180-8keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
394-405issue
2eissn
0014-4886issn
1090-2430pii
S0014488603001808journal_volume
183pub_type
杂志文章abstract：:l-DOPA therapy in Parkinson's disease often results in side effects such as l-DOPA-induced dyskinesia (LID). Our previous studies demonstrated that defective desensitization of dopamine receptors caused by decreased expression of G protein-coupled receptor kinases (GRKs) plays a role. Overexpression of GRK6, the isofo...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2015.02.008
更新日期：2015-04-01 00:00:00
abstract：:The role of microglia and their contribution to the development and maintenance of pain states has emerged as an attractive field of study. Sensitization of central nociceptors and interneurons is thought to be responsible for the symptoms of chronic neuropathic pain states. Microglia interact with these neurons at th...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2011.12.021
更新日期：2012-04-01 00:00:00
abstract：:The failure of axons to regenerate after spinal cord injury remains one of the greatest challenges facing both medicine and neuroscience, but in the last 20 years there have been tremendous advances in the field of spinal cord injury repair. One of the most important of these has been the identification of inhibitory ...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2007.06.020
更新日期：2008-02-01 00:00:00
abstract：:Hypernatremic dehydration was induced in rabbits during a 3- to 5-day period resulting in mean plasma sodium concentrations of 187 meq/liter. The animals were then rehydrated during a 4-h period by intravenous administration of a 2.5% glucose or fructose solution. The water content of four regions of brain sample show...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90215-8
更新日期：1985-02-01 00:00:00
abstract：:A "neuroplastic" hypothesis proposes that changes in neuronal structural plasticity may underlie the aetiology of depression and the action of antidepressants. The medial prefrontal cortex (mPFC) is affected by this disorder and shows an intense expression of the polysialylated form of the neural cell adhesion molecul...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2008.07.018
更新日期：2008-11-01 00:00:00
abstract：:Hyperreflexia develops after spinal cord injury (SCI) in the human and in the spinal cord transected animal, and can be measured by the loss of low frequency-dependent depression of the H-reflex. Previous studies demonstrated normalization of low frequency-dependent depression of the H-reflex using passive exercise wh...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2008.07.002
更新日期：2008-10-01 00:00:00
abstract：:Peripheral nerve regeneration following injury occurs spontaneously, but many of the processes require metabolic energy. The mechanism of energy supply to axons has not previously been determined. In the central nervous system, monocarboxylate transporter 1 (MCT1), expressed in oligodendroglia, is critical for supplyi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2014.10.018
更新日期：2015-01-01 00:00:00
abstract：:Parameters related to the capacity and the rate of uptake of calcium ions by the sarcoplasmic reticulum were measured in skinned extensor digitorum longus fibers of control and myodystrophic mice. Single fibers were isolated and skinned in a relaxing solution and mounted on a force transducer and apparatus for changin...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90140-2
更新日期：1985-01-01 00:00:00
abstract：:Roles of protein kinases in mediating adaptive neuronal responses to activation of signal transduction pathways are well known. Recent findings suggest that kinases may also be involved in pathological processes in the nervous system. The present study employed cultured human cerebral cortical neurons to test the hypo...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(91)90118-v
更新日期：1991-04-01 00:00:00
abstract：:There is compelling evidence that excessive exposure to manganese (Mn) produces neurotoxicity, especially in the basal ganglia, resulting in a dystonic Parkinsonian disorder. Several experimental or clinical observations suggest that Mn neurotoxicity could involve impairment of energy metabolism. We examined the neuro...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1993.1042
更新日期：1993-03-01 00:00:00
abstract：:Two neutral alpha-glucosidase isoenzymes were isolated from the muscle of Japanese quails with late-onset acid maltase deficiency. One isoenzyme is predominantly expressed in embryonic muscle and the other in adult muscle. The time of switching from one to the other of these two neutral alpha-glucosidases was the same...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(88)90117-3
更新日期：1988-05-01 00:00:00
abstract：:Olfactory ensheathing cells (OECs) are unique glia that support axon outgrowth in the olfactory system, and when used as cellular therapy after spinal cord injury, improve recovery and axon regeneration. Here we assessed the effects of combining OEC transplantation with another promising therapy, epidural electrical s...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2018.07.015
更新日期：2018-11-01 00:00:00
abstract：:Transplantation of genetically engineered cells can provide sustained focal delivery of naturally occurring molecules, including neurotransmitters and growth factors. We have engineered immortalized mouse cortical neurons and glia to deliver GABA by driving GAD(65) expression. Engineered cell lines showed GAD(65) mRNA...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1999.7305
更新日期：2000-02-01 00:00:00
abstract：:Electrical stimulation of the midbrain tegmentum can produce differential modulation of quiet biting attack and affective defense behavior elicited from the hypothalamus of the cat. Stimulation of the lateral half of the tegmentum facilitated quiet biting attack and suppressed affective defense. Conversely, stimulatio...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/S0014-4886(84)90112-2
更新日期：1984-02-01 00:00:00
abstract：:The present study was carried out to investigate whether estrogen modulates the drinking response induced by activation of angiotensinergic neural pathways from the subfornical organ (SFO) to the median preoptic nucleus (MnPO). Microinjection of angiotensin II (ANG II, 10(-10) M, 0.2 microl) into the SFO elicited drin...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2002.8054
更新日期：2003-01-01 00:00:00
abstract：:Diabetes mellitus is a common metabolic disease in human beings with characteristic symptoms of hyperglycemia, chronic inflammation and insulin resistance. One of the most common complications of early-onset diabetes mellitus is peripheral diabetic neuropathy, which is manifested either by loss of nociception or by al...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2015.06.022
更新日期：2015-09-01 00:00:00
abstract：:Minocycline is neuroprotective in many experimental models of neurodegenerative diseases and central nervous system (CNS) injury but has not previously been tested in a model of viral encephalitis. Experimental infection of neonatal mice with neurotropic reoviruses is a classic model for studying the pathogenesis of v...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2004.11.015
更新日期：2005-04-01 00:00:00
abstract：:A novel model of status epilepticus based on the kindling model of epilepsy is described. The model involves the administration of a small dose of pilocarpine (20 mg/kg) to rats that have been previously kindled. Stimulation of these pretreated rats produces seizures which continue uninterrupted for approximately 4 h ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(86)90274-8
更新日期：1986-10-01 00:00:00
abstract：:Monosialoganglioside GM1 prevents excitatory amino acid (EAA)-related neuronal death in cultured central nervous system (CNS) neurons and reduces the severity of acute brain damage in different experimental models of cerebral ischemia. Using a model of brain damage induced by intracerebroventricular administration of ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(91)90019-9
更新日期：1991-09-01 00:00:00
abstract：:Human recombinant activated factor-VII (rFVIIa) has been used successfully in the treatment of spontaneous intracerebral hemorrhage. In addition, there is increasing interest in its use to treat uncontrolled bleeding of other origins, including trauma. The aim of this study was to evaluate the safety and potential eff...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2007.12.019
更新日期：2008-04-01 00:00:00
abstract：:Huntington's disease (HD) is a progressive neurodegenerative disorder characterized by chorea, psychiatric disturbances, and dementia. It is caused by a polyglutamine repeat expansion in the huntingtin protein. The striatum is a major site of neuronal loss in HD, but the mechanisms underlying the neurodegenerative pro...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2000.7434
更新日期：2000-09-01 00:00:00
abstract：:Cerebral ischemia caused by loss of blood supply to the brain during cardiac arrest or stroke are major causes of death and disability. Biological sex is an important factor in predicting vulnerability of the brain to an ischemic insult, with males being at higher risk for cardio-cerebrovascular events than females of...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2014.02.004
更新日期：2014-09-01 00:00:00
abstract：:Vasoactive intestinal peptide (VIP) is one of the neuropeptides showing the strongest up-regulation in the nociceptive pathway after peripheral nerve injury and has been proposed to support neuropathic pain. Nevertheless, the story may be more complicated considering the known suppressive effects of the peptide on the...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2017.03.019
更新日期：2017-07-01 00:00:00
abstract：:Although aluminum (Al) contributes to a variety of cognitive dysfunctions and mental diseases, the underlying mechanisms of Al interactions with the nervous system are still unknown. We have studied the action of Al on synaptic transmission and long-term potentiation (LTP) by performing electrophysiological recordings...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1995.1038
更新日期：1995-07-01 00:00:00
abstract：:Previous two-dimensional (2-D) gel electrophoretic studies of proteins secreted by degenerating mammalian peripheral nerves (Ignatius et al., 1986, Proc. Natl. Acad. Sci. USA 83: 1125-1129; Muller et al., 1986, J. Cell Biol. 102: 393-402) detected the up-regulation of two proteins of 67-70 and 34-37 kDa, although they...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1999.7332
更新日期：2000-03-01 00:00:00
abstract：:Seizures were induced in immature 18-day-old rats by i.p. administration of homocysteine (11 mmol/kg) and the effects of selected antagonists of NMDA receptors [MK-801 (0.5 mg/kg), AP7 (0.33 mmol/kg), CGP 40116 (10 mg/kg)] and non-NMDA receptors [GDEE (4 mmol/kg), NBQX (two doses, 30 mg/kg each)] were studied. The eff...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6464
更新日期：1997-06-01 00:00:00
abstract：:To test the hypothesis that prepubertal exposure of LHRH neurons to a source of catecholamines and neuropeptides accelerates the onset of puberty, we examined the effects of autologous adrenal transplantation into the base of the third ventricle of the brain in juvenile female rhesus monkeys at 11-13 months of age. Th...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1996.0127
更新日期：1996-08-01 00:00:00
abstract：:After spinal cord injury (SCI), the majority of individuals develop spasticity, a debilitating condition involving involuntary movements, co-contraction of antagonistic muscles, and hyperreflexia. By acting on GABAergic and Ca2+-dependent signaling, current anti-spastic medications lead to serious side effects, includ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2021.113605
更新日期：2021-01-13 00:00:00
abstract：:Systemic administration of the amphetamine derivative p-chloroamphetamine (PCA) causes degeneration of 5-HT axon terminals in rat brain. The present study was designed to determine whether PCA induces neurotoxic effects by a direct action on 5-HT axon terminals. PCA was administered by microinjection directly into the...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/s0014-4886(05)80015-9
更新日期：1990-09-01 00:00:00
abstract：:Cells dissociated from 6-day rat cerebellum were seeded on glass coverslips coated with polylysine on one half and hyaluronectin on the other. Cell attachment and neurite extension were drastically reduced on hyaluronectin-coated surfaces. ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(88)90218-x
更新日期：1988-04-01 00:00:00
