Abstract:
:A monoclonal antibody (mAb), 2E12, against the neural cell adhesion molecule L1 recognized the 200 kDa component of L1. The epitope of L1 reacting with mAb 2E12 was localized in its carbohydrate chain, judging from the results of experiments on glycopeptidase F treatment. The physiological effect of adding mAbL1 (2E12) to cultured mouse dorsal root ganglion neurons was studied using patch-clamp techniques. The binding of mAbL1 (2E12) to the neurons expressing L1 molecule induced an inward current inhibited by calcium channel blockers such as nifedipine and Lanthanum. It was also found that the mAbL1 (2E12) leads to a rise in the intracellular Ca2+ concentration ([Ca2+]i) in cultured neurons. This rise seems to be due to an influx of extracellular Ca2+, since treatment with EGTA abolished those phenomena. L-type calcium channel blockers such as nifedipine and cadmium, as well as inward current, blocked the effect of mAbL1 (2E12). These results suggest that the carbohydrate chain of L1 glycoprotein is directly involved in the induction of calcium current, and that the L1 molecule may play a prominent role in regulation of the Ca2+ channel.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Asou Hdoi
10.1002/jcp.1041530211keywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
313-20issue
2eissn
0021-9541issn
1097-4652journal_volume
153pub_type
杂志文章abstract::In this review, we focused our attention on the more important natural extracellular matrix (ECM) molecules (collagen and fibrin), employed as cellular scaffolds for tissue engineering and on a class of semi-synthetic materials made from the fusion of specific oligopeptide sequences, showing biological activities, wit...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.20270
更新日期:2005-06-01 00:00:00
abstract::A novel, serum albumin-binding glycoprotein of molecular weight (mw) 43,000 (43K protein) was initially purified from the culture medium of bovine aortic endothelial (BAE) cells (Sage, H., Johnson, C., and Bornstein, P., J. Biol. Chem. 259:3993-4007, 1984). Its secretion by normal mesenchymal cells and by transformed ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041270305
更新日期:1986-06-01 00:00:00
abstract::We have studied the transcriptional activity, steady-state mRNA levels, and steady-state protein levels of the c-myc and transferrin receptor (TfR) genes in murine M1 myeloid leukemia cells arrested in G1 phase of the cell cycle by different methods. When cells are growth-arrested by density inhibition, a technique th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041350223
更新日期:1988-05-01 00:00:00
abstract::Signal transduction of fibroblast growth factor (FGF) receptors is known to involve tyrosine phosphorylation of several substrates, including Grb2, phospholipase C-gamma, and phosphatidylinositol 3-kinase, whereas the role of G-proteins in FGF receptor signaling is controversial. In the present study we investigated t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199604)167:1<52::AID-JCP6>
更新日期:1996-04-01 00:00:00
abstract::This study was to explore a potential role of epithelium-derived cytokines in Th17 differentiation. Th17 induction was evaluated by murine CD4(+) T cells treated with different combinations of five inducing cytokines, or conditioned media of human corneal epithelial cells (HCECs) exposed to a variety of stimuli. Th17 ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21926
更新日期:2010-01-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) play pivotal roles in bone and cartilage growth and repair. Through phenotypes of short-ear (se) mice, which have BMP-5 mutations, a role for BMP-5 in some specific aspects of skeletogenesis and cartilage growth is known. This report examines BMP-5 expression in the growth plate and ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21164
更新日期:2008-01-01 00:00:00
abstract::NAD(P) is an indispensable cofactor for all organisms and its biosynthetic pathways are proposed as promising novel antibiotics targets against pathogens such as Mycobacterium tuberculosis. Six NAD(P) biosynthetic pathways were reconstructed by comparative genomics: de novo pathway (Asp), de novo pathway (Try), NmR pa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.22419
更新日期:2011-02-01 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is prevalent chronic liver diseases with unknown mechanism and no curative treatment. Hepatokines have demonstrated importance in NAFLD but, role of selenoprotein P (SeP) in NAFLD is unknown. A total of 79 patients with NAFLD and 79 healthy controls were included in this case-c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30121
更新日期:2020-10-22 00:00:00
abstract::Schizophrenia (SZ) is characterized by a high morbidity and disability rate and has gradually increased in rate and caused much burden. However, the pathogenesis of SZ is elusive and may include changes in the biological molecules in exosomes. In this study, we first compared the alterations of plasma exosomal circula...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29873
更新日期:2021-01-01 00:00:00
abstract::This study examines the mechanism by which TGF-beta 1, an important mediator of cell growth and differentiation, blocks the differentiation of normal rat diploid fetal osteoblasts in vitro. We have established that the inability for pre-osteoblasts to differentiate is associated with changes in the expression of cell ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041600214
更新日期:1994-08-01 00:00:00
abstract::Interleukin-10 (IL-10) has been shown to exert anti-inflammatory effects by suppressing macrophage proliferation and inhibiting cytokine production. In this study we show that in the presence of erythropoietin (EPO), the addition of IL-10 results in a significant dose-dependent increase in both Burst Forming Unit-Eryt...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199602)166:2<305::AID-JCP8
更新日期:1996-02-01 00:00:00
abstract::Low-power laser irradiation (LPLI) has been shown to exert promotive effects on cell survival and proliferation through activation of various signaling pathways. Estrogen receptors (ERs, ERα, and ERβ) are ligand-activated transcription factors, which regulate target gene expression, promote cell proliferation, and res...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24252
更新日期:2013-05-01 00:00:00
abstract::Drug discovery is an onerous, extremely expensive, and time-consuming process. Instead, drug repurposing is an attractive strategy for exploiting novel indications for a drug beyond its original use. The untapped potential of drug repurposing compensates the barriers associated with the drug discovery pipeline. Psoria...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29413
更新日期:2020-06-01 00:00:00
abstract::Adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cells isolated from adipose tissue and have the ability to differentiate into adipogenic, osteogenic, and chondrogenic lineages. Despite their great therapeutic potentials, previous studies showed that ADSCs could enhance the proliferation and metastati...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29574
更新日期:2020-10-01 00:00:00
abstract::Interleukin-34 (IL-34) shares a common receptor with macrophage colony-stimulating factor (M-CSF), and can bind to CSF-1R, induces lymphocytes differentiation, proliferation, and regulates the synthesis of inflammatory components. Recent findings reported aberrant expression of IL-34 in several autoimmune disorders, s...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28946
更新日期:2019-12-01 00:00:00
abstract::While retinoids have been demonstrated to inhibit growth of many tumor cells, including SCC cells, the molecular mechanism by which retinoids suppress growth has not been elucidated. We previously found that the growth of SCC cells was significantly inhibited by all-trans-retinoic acid (all-trans-RA) treatment, and th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10051
更新日期:2002-03-01 00:00:00
abstract::Mesenchymal stem cells (MSC) represent emerging cell-based therapies for diabetes and associated complications. Ongoing clinical trials are using exogenous MSC to treat type 1 and 2 diabetes, cardiovascular disease and non-healing wounds due to diabetes. The majority of these trials are aimed at exploiting the ability...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24013
更新日期:2012-09-01 00:00:00
abstract::Males and females differ across a broad spectrum of morphological, physiological, and behavioral characters. In fact, sexually dimorphic traits typically contribute the largest component of phenotypic variance in most taxa that use sex to reproduce. However, we know very little about the mechanisms that maintain these...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24979
更新日期:2015-10-01 00:00:00
abstract::Core binding factor β (Cbfβ) is a partner protein of Runx family transcription factors with minimally characterized function in cartilage. Here we address the role of Cbfβ in cartilage by generating chondrocyte-specific Cbfβ-deficient mice (Cbfb(Δch/Δch) ) from Cbfb-floxed mice crossed with mice expressing Cre from th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25068
更新日期:2016-01-01 00:00:00
abstract::Incubation of cultured bovine pulmonary artery endothelial cells with 200 microM of 3-isobutyl-1-methylxanthine (IBMX) for 24 hr produced a five- to tenfold increase in cellular angiotensin converting enzyme activity (ACE) above that of untreated control cells. A lesser increase was observed in medium ACE. Other methy...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041370122
更新日期:1988-10-01 00:00:00
abstract::The Schneider membrane is the mucosa that covers the inner part of the maxillary sinus cavities. The free surface is a ciliated pseudostratified epithelium, while the deeper portion is a highly vascularized connective tissue. The stromal fraction, bordering the bony wall of the sinus, after tooth loss can exhibit incr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24022
更新日期:2012-09-01 00:00:00
abstract::Peroxisome proliferator-activated receptor-γ coactivator-1 deficient (Pgc-1β-/- ) murine hearts model the increased, age-dependent, ventricular arrhythmic risks attributed to clinical conditions associated with mitochondrial energetic dysfunction. These were accompanied by compromised action potential (AP) upstroke ra...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27183
更新日期:2019-04-01 00:00:00
abstract::Vitamin E, a dietary antioxidant, is presumed to be incorporated into the lipid bilayer of biological membranes to an extent proportional to the amount of polyunsaturated fatty acids or phospholipids in the membrane. In the present study we evaluated the distribution of incorporated polyunsaturated fatty acids (PUFA) ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041450324
更新日期:1990-12-01 00:00:00
abstract::There is strong evidence that thyroid hormones through triiodothyronine (T3) regulate Sertoli cell proliferation and differentiation in the neonatal testis. However, the mechanism(s) by which they are able to control Sertoli cell proliferation is unclear. In the present study in vivo approaches (PTU-induced neonatal h...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20716
更新日期:2006-10-01 00:00:00
abstract::Temperature-sensitive mutants have revealed a region of the herpes simplex virus 1 genome that affects both the uptake of hexose and the synthesis of heat shock proteins. Other inducers of heat-shock proteins, namely heat shock itself and arsenite, likewise induce an increased uptake of hexose. The increased uptake, l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041280306
更新日期:1986-09-01 00:00:00
abstract::Photoperiod regulates the seasonal reproductive rhythms of mammals by influencing the development and function of sexual organs; however, the underlying mechanism remains unclear. We examined the morphology and functioning of the main sex organs of striped dwarf hamsters (Cricetulus barabensis) under different photope...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29998
更新日期:2021-03-01 00:00:00
abstract::The nuclear Ki-67 protein (pKi-67) has previously been shown to be exclusively expressed in proliferating cells. As a result, antibodies against this protein are widely used as prognostic tools in cancer diagnostics. Here we show, that despite the strong downregulation of pKi-67 expression in non-proliferating cells, ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20494
更新日期:2006-03-01 00:00:00
abstract::Spinal cord injury (SCI) is a public health problem in the world. The SCI usually triggers an excessive inflammatory response that brings about a secondary tissue wreck leading to further cellular and organ dysfunction. Hence, there is great potential of reducing inflammation for therapeutic strategies of SCI. In this...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28124
更新日期:2019-08-01 00:00:00
abstract::The present study was designed to investigate the mechanism of myeloid differentiation protein 2 (MD2) on intestinal mucosa destruction in mice with chronic colitis. Briefly, a chronic colitis mouse model was established by the administration of dextran sulfate sodium (DSS) in transgenic mice of MD2 overexpression (Tr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28711
更新日期:2019-11-01 00:00:00
abstract::Gastric cancer has the fifth highest incidence of disease and is the third leading cause of cancer-associated mortality in the world. The etiology of gastric cancer is complex and needs to be fully elucidated. Thus, it is necessary to explore potential pathogenic genes and pathways that contribute to gastric cancer. G...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29177
更新日期:2020-03-01 00:00:00