Abstract:
:Previous research in this laboratory, using photoactivatable radioiodinated lipopolysaccharide derivatized with sulfosuccinimidyl-2-(p-azidosalicylamide)-1,3'-dithiopropionate (125I-ASD-LPS), has resulted in the identification of a specific LPS receptor with a molecular mass of approximately 73 kDa on murine lymphocytes and splenic macrophages. The experiments presented in this report investigated whether a similar LPS-binding protein was also expressed on human peripheral blood populations, including monocytes, lymphocytes, neutrophils, platelets, and erythrocytes. Each cell population was incubated with 125I-ASD-LPS, UV irradiated, washed, reduced, and solubilized, and the cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography. On all of the cell populations, except erythrocytes, a similar 73-kDa LPS-binding protein was present. In addition, each population also expressed lower-molecular-weight secondary LPS-binding proteins, some of which were conserved among the populations. Binding of the photoactivatable LPS probe was found to be both time and temperature dependent. These data support the concept that the 73-kDa LPS-binding protein is conserved on multiple cell types from a variety of species.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Halling JL,Hamill DR,Lei MG,Morrison DCdoi
10.1128/IAI.60.3.845-852.1992keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
845-52issue
3eissn
0019-9567issn
1098-5522journal_volume
60pub_type
杂志文章abstract::The leukotrienes and tumor necrosis factor (TNF) play an important role in the pathophysiology of septic shock, in which hypotension, leukopenia, thrombocytopenia, and hemoconcentration are observed. This study was performed to examine the effects of a 5-lipoxygenase inhibitor (AA-861), a selective leukotriene recepto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2432-2437.1992
更新日期:1992-06-01 00:00:00
abstract::The cellular source of interleukin-6 (IL-6) during infection of mice with Listeria monocytogenes was investigated both in vitro and in vivo. Peritoneal cells taken at intervals from infected mice and cultured in vitro without added stimulus produced high titers of IL-6 peaking 2 days postinfection in a time course sim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2626-2631.1993
更新日期:1993-06-01 00:00:00
abstract::Saccharomyces boulardii, a nonpathogenic yeast, is effective in treating some patients with Clostridium difficile diarrhea and colitis. We have previously reported that S. boulardii inhibits rat ileal secretion in response to C. difficile toxin A possibly by releasing a protease that digests the intestinal receptor fo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5225-5232.1996
更新日期:1996-12-01 00:00:00
abstract::Mannan (MAN) extracted from Candida albicans 20A was investigated for its potential as an antigen in the detection of cell-mediated immunity (CMI) in vivo and in vitro and for its ability to modulate CMI when administered intravenously (i.v.). CBA/J mice were either immunized as adults by the cutaneous inoculation of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.693-700.1989
更新日期:1989-03-01 00:00:00
abstract::Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of delivery, and epithelial cells were dissociated from the amniotic membranes by trypsinization and grown in Rose chambers. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2042-2047.1990
更新日期:1990-07-01 00:00:00
abstract::Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2416-2419.1987
更新日期:1987-10-01 00:00:00
abstract::Although it has been known for some time that Salmonella typhimurium is able to survive and even replicate in the normally bactericidal environment of the macrophage phagosome, the mechanisms by which this organism accomplishes this feat remain obscure. In this study, a murine macrophage cell line and confocal immunof...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1475-1485.1997
更新日期:1997-04-01 00:00:00
abstract::Buruli ulcer, caused by Mycobacterium ulcerans, is characterized by deep and necrotizing skin lesions, mostly on the arms and legs. Together with tuberculosis and leprosy, this mycobacterial disease has become a major health problem in tropical and subtropical regions, particularly in central and western Africa. No sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5403-5411.2001
更新日期:2001-09-01 00:00:00
abstract::The possibility that preexisting specific serum antibody could suppress a defined mucosal immune response to a topically applied antigen was studied in rats. Hyperimmune serum antibody induced by parenteral immunization of rats with cholera toxoid markedly suppressed the mucosal immune response to enterically applied ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.30.1.62-68.1980
更新日期:1980-10-01 00:00:00
abstract::Current evidence indicates that Mycobacterium avium infection in patients with AIDS is acquired mostly through the gastrointestinal (GI) tract and that M. avium binds to and invades GI mucosal cells in vitro. Since M. avium is exposed to specific environmental conditions in the GI tract such as changes in pH, low oxyg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3768-3773.1997
更新日期:1997-09-01 00:00:00
abstract::We compared the immune defense of mice with chronic granulomatous disease (CGD mice) with that of wild-type C57BL/6 mice for their response to Sporothrix schenckii. A subcutaneous injection of 5 x 10(4) CFU S. schenckii strain IFM41598 into CGD mice resulted in systemic infection and death within 84 days. In contrast,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5073-5079.2004
更新日期:2004-09-01 00:00:00
abstract::Brucella spp. are gram-negative intracellular pathogens that survive and multiply within phagocytic cells of their hosts. Smooth organisms present O polysaccharides (OPS) on their surface. These OPS help the bacteria avoid the bactericidal action of serum. The wboA gene, coding for the enzyme glycosyltransferase, is e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4407-4416.2001
更新日期:2001-07-01 00:00:00
abstract::Routine culture of endothelial cells currently includes the use of heparin, which significantly reduces cell doubling time and increases cell population size. Heparin protects cultured arterial endothelial cells from damage by toxic oxygen metabolites produced by the action of xanthine and xanthine oxidase. Because of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4505-4510.1991
更新日期:1991-12-01 00:00:00
abstract::Incubation of INT407 cells with various clinical isolates of Campylobacter jejuni resulted in secretion of interleukin-8 (IL-8) at levels ranging from 96 to 554 pg/ml at 24 h. The strains which produced the highest levels of IL-8 secretion were 81-176 and BT44. Induction of IL-8 secretion required live cells of 81-176...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.88-93.1999
更新日期:1999-01-01 00:00:00
abstract::We have characterized the natural immune responses to the 19-kDa domain of merozoite surface protein 1 in individuals from an area of western Kenya in which malaria is holoendemic. We used the three known natural variant forms of the yeast-expressed recombinant 19-kDa fragment that are referred to as the E-KNG, Q-KNG,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2716-2723.1996
更新日期:1996-07-01 00:00:00
abstract::We previously showed the requirement of both T cells and gamma interferon (IFN-gamma)-producing non-T cells for the genetic resistance of BALB/c mice to the development of toxoplasmic encephalitis (TE). In order to define the role of IFN-gamma production and the perforin-mediated cytotoxicity of T cells in this resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4432-4438.2004
更新日期:2004-08-01 00:00:00
abstract::Recently, our laboratory and others have demonstrated that human peripheral blood T and NK lymphocytes directly inhibit the growth of Cryptococcus neoformans. In this study, we further define the conditions under which lymphocyte-mediated fungistasis against C. neoformans occurs and examine whether mechanisms implicat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3550-3554.1995
更新日期:1995-09-01 00:00:00
abstract::The essential role of T cells in the resolution of primary murine Chlamydia trachomatis genital tract infection is inarguable; however, much less is known about the mechanisms that confer resistance to reinfection. We previously established that CD4+ T cells and B cells contribute importantly to resistance to reinfect...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2643-2649.2001
更新日期:2001-04-01 00:00:00
abstract::Host-driven macrophage apoptosis contributes to innate immunity during bacterial infection. Neisseria meningitidis inhibits apoptosis in a variety of cells, but its impact on macrophage apoptosis is unknown. We demonstrate that N. meningitidis prevents macrophage apoptosis via genes encoding nitric oxide detoxificatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.729-733.2006
更新日期:2006-01-01 00:00:00
abstract::Three monoclonal antibodies (MAbs), E4, L1-4, and L1-24, to the major outer membrane protein (MOMP) of Chlamydia trachomatis were identified that neutralized in vitro the infectivity of members of the B- and C-related complex as well as the mouse pneumonitis strain. MAbs L1-4, L1-24, and E4 gave a strong signal in an ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.11.4147-4153.1991
更新日期:1991-11-01 00:00:00
abstract::We describe here a Sprague-Dawley rat model for chronic osteomyelitis. Staphylococcus aureus and sodium morrhuate were implanted by either microdrilling or direct needle injection into the tibiae of rats. Of 107 rats, 87 (81%) developed osteomyelitis when a high-speed drill was used for implantation, and 27 (51%) of 5...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.581-586.1985
更新日期:1985-03-01 00:00:00
abstract::An immunomodulatory role of arthropod saliva has been well documented, but evidence for an effect on Plasmodium sp. infectiousness remains controversial. Mosquito saliva may orient the immune response toward a Th2 profile, thereby priming a Th2 response against subsequent antigens, including Plasmodium. Orientation to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06414-11
更新日期:2012-06-01 00:00:00
abstract::Virulent Treponema pallidum organisms, extracted from infected rabbit testes, were subjected to velocity sedimentation in discontinuous gradients of Hypaque, a high density, low viscosity material. After centrifugation of extracts at 20 C for 45 min at 100,000 x g, treponemes separated into two distinct bands based up...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1062-1067.1974
更新日期:1974-11-01 00:00:00
abstract::Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. Bovine NRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3110-3119.2001
更新日期:2001-05-01 00:00:00
abstract::Chlamydia trachomatis is an obligate intracellular pathogen that causes sexually transmitted disease. In women, chlamydial infections may cause pelvic inflammatory disease (PID), ectopic pregnancy, and infertility. The role of antibodies in protection against a primary Chlamydia infection is unclear and was a focus of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00413-20
更新日期:2020-12-15 00:00:00
abstract::Acquired cellular immunity to infection with Mycobacterium tuberculosis is believed to reside in the capacity of mononuclear phagocytes of immunized animals to inhibit intracellular multiplication of the parasite. However, in macrophage tissue culture systems, it has been customary to employ streptomycin in the medium...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-01-01 00:00:00
abstract::The in vivo role of endogenous interleukin 12 (IL-12) in modulating intrapulmonary growth of Legionella pneumophila was assessed by using a murine model of replicative L. pneumophila lung infection. Intratracheal inoculation of A/J mice with virulent bacteria (10(6) L. pneumophila cells per mouse) resulted in inductio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.65-69.1998
更新日期:1998-01-01 00:00:00
abstract::The pneumocandins are potent antifungal agents of the echinocandin class which are under development for use as broad-spectrum antimycotic therapy. One important consideration for any new therapeutic class for treating serious fungal infections is the potential for drug resistance development. In this study we have is...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3244-3251.1996
更新日期:1996-08-01 00:00:00
abstract::Chromosomal DNA from a uropathogenic strain of Escherichia coli was partially digested with the restriction enzyme EcoRI. The partial digests were ligated into a cosmid containing an ampicillin-resistant determinant and packaged into lambda phage particles. An ampicillin-resistant transductant of E. coli HB101 was fou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.739-744.1982
更新日期:1982-11-01 00:00:00
abstract::Intrauterine infections lead to serious complications for mother and fetus, including preterm birth, maternal and fetal death, and neurological sequelae in the surviving offspring. Improving maternal and child heath is a global priority. Yet, the development of strategies to prevent and treat pregnancy-related disease...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00801-17
更新日期:2018-05-22 00:00:00