Functional mutants of the sequence-specific transcription factor p53 and implications for master genes of diversity.

abstract：:The Na/K pump is a P-type ATPase that exchanges three intracellular Na(+) ions for two extracellular K(+) ions through the plasmalemma of nearly all animal cells. The mechanisms involved in cation selection by the pump's ion-binding sites (site I and site II bind either Na(+) or K(+); site III binds only Na(+)) are po...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratheal IM,Virgin GK,Yu H,Roux B,Gatto C,Artigas P

更新日期：2010-10-26 00:00:00

abstract：:We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dirks RM,Pierce NA

更新日期：2004-10-26 00:00:00

abstract：:Half-cystine peptic peptides representing the intrachain disulfide bonds of the light chain from an apparently homogeneous rabbit anti-p-azobenzoate antibody were isolated in good yields after reduction and alkylation with [(14)C]iodoacetate, and their sequences were determined. The sequence of the first 21 amino-term...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Appella E,Chersi A,Roholt OA,Pressman D

更新日期：1971-10-01 00:00:00

abstract：:Understanding the gene-expression patterns during liver regeneration may help to reveal how regenerative processes are initiated and controlled as well as shed new light onto processes that lead to liver disease. Using high-density oligonucleotide arrays, we have examined the gene-expression program in the livers of m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Su AI,Guidotti LG,Pezacki JP,Chisari FV,Schultz PG

更新日期：2002-08-20 00:00:00

abstract：:In solid-state physics, "doping" is a pivotal concept that allows controlling and engineering of the macroscopic electronic and optical properties of materials such as semiconductors by judiciously introducing small concentrations of impurities. Recently, this concept has been translated to two-dimensional photonic sc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coppolaro M,Moccia M,Castaldi G,Engheta N,Galdi V

更新日期：2020-06-23 00:00:00

abstract：:Combined intracellular and microfluorometric recording techniques were used to evaluate whether the inhibition by cholinergic or adrenergic transmitters of the Ca2(+)-activated potassium current (IAHP) in hippocampal CA3 pyramidal cells was mediated by an alteration of depolarization-induced change in cytosolic free C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knöpfel T,Vranesic I,Gähwiler BH,Brown DA

更新日期：1990-06-01 00:00:00

abstract：:Exposure of 3T3/A31 cells to serum-free medium, one type of apoptotic stimulus, causes a rapid increase in the sphingosine (Sph) level, which initiates a series of processes: (i) activation of caspase 3 through an enhanced "cascade" of caspases, (ii) release of the C-terminal-half kinase domain of PKCdelta (PKCdelta K...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suzuki E,Handa K,Toledo MS,Hakomori S

更新日期：2004-10-12 00:00:00

abstract：:The relationship between performance and ability is a central concern in the social sciences: Are the most successful much more able than others, and are failures unskilled? Prior research has shown that noise and self-reinforcing dynamics make performance unpredictable and lead to a weak association between ability a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Denrell J,Liu C

更新日期：2012-06-12 00:00:00

abstract：:Although genetic analysis has demonstrated that members of the winged helix, or forkhead, family of transcription factors play pivotal roles in the regulation of cellular differentiation and proliferation, both during development and in the adult, little is known of the mechanisms underlying their regulation. Here we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biggs WH 3rd,Meisenhelder J,Hunter T,Cavenee WK,Arden KC

更新日期：1999-06-22 00:00:00

abstract：:The configurations of the hydrocarbon chains in micelles are severely constrained by the space-filling requirements of the chain segments, by the continuity of the chains, and by the micellar geometry. A statistical theory that takes full account of these constraints is developed by using a lattice model. The chain en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dill KA,Flory PJ

更新日期：1981-02-01 00:00:00

abstract：:Within the basal ganglia, acetylcholine and dopamine play a central role in the extrapyramidal control of motor function. The physiologic effects of these neurotransmitters are mediated by a diversity of receptor subtypes, several of which have now been cloned. Muscarinic acetylcholine receptors are encoded by five ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiner DM,Levey AI,Brann MR

更新日期：1990-09-01 00:00:00

abstract：:An Escherichia coli chromosomal DNA fragment cloned on a multicopy plasmid conferred resistance to carbonylcyanide m-chlorophenylhydrazone, nalidixic acid, and a number of other toxic compounds. The sequence of the cloned emr locus located at minute 57.5 of the chromosome revealed two open reading frames, emrA and emr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lomovskaya O,Lewis K

更新日期：1992-10-01 00:00:00

abstract：:(3)H-actinomycin D, a guanine-binding agent, labels fixed human chromosomes nonrandomly. Actinomycin D added in G2 inhibits secondary constrictions and breaks chromosomes. There is some tendency for label to be concentrated at the ends of chromosomes and near the centromere. Labeling with (3)H-thymidine in the late st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles CP

更新日期：1970-03-01 00:00:00

abstract：:Cerebral organization during sentence processing in English and in American Sign Language (ASL) was characterized by employing functional magnetic resonance imaging (fMRI) at 4 T. Effects of deafness, age of language acquisition, and bilingualism were assessed by comparing results from (i) normally hearing, monolingua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neville HJ,Bavelier D,Corina D,Rauschecker J,Karni A,Lalwani A,Braun A,Clark V,Jezzard P,Turner R

更新日期：1998-02-03 00:00:00

abstract：:Gene knockout is the most powerful tool for determining gene function or permanently modifying the phenotypic characteristics of a cell. Existing methods for gene disruption are limited by their efficiency, time to completion, and/or the potential for confounding off-target effects. Here, we demonstrate a rapid single...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santiago Y,Chan E,Liu PQ,Orlando S,Zhang L,Urnov FD,Holmes MC,Guschin D,Waite A,Miller JC,Rebar EJ,Gregory PD,Klug A,Collingwood TN

更新日期：2008-04-15 00:00:00

abstract：:The expression of the human HSP70 gene is induced by a wide range of physiological stresses, including exposure to heat shock and heavy metals, or under nonstress conditions, such as in response to serum stimulation. We have previously demonstrated that in either case the regulated expression is at the primary level o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu BJ,Kingston RE,Morimoto RI

更新日期：1986-02-01 00:00:00

abstract：:Voltage-gated Na(+) channels initiate action potentials during electrical signaling in excitable cells. Opening and closing of the pore of voltage-gated ion channels are mechanically linked to voltage-driven outward movement of the positively charged S4 transmembrane segment in their voltage sensors. Disulfide locking...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeCaen PG,Yarov-Yarovoy V,Scheuer T,Catterall WA

更新日期：2011-11-15 00:00:00

abstract：:Current understanding of the integration of cell division and expansion in the development of plant lateral organs such as leaves is limited. Cell number is established during a mitotic phase, and subsequent growth into a mature organ relies primarily on cell expansion accompanied by endocycles. Here we show that the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dewitte W,Scofield S,Alcasabas AA,Maughan SC,Menges M,Braun N,Collins C,Nieuwland J,Prinsen E,Sundaresan V,Murray JA

更新日期：2007-09-04 00:00:00

abstract：:Electron spin echo electron-nuclear double resonance (ESE-ENDOR) experiments performed on a broad radical electron paramagnetic resonance (EPR) signal observed in photosystem II particles depleted of Ca2+ indicate that this signal arises from the redox-active tyrosine YZ. The tyrosine EPR signal width is increased rel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gilchrist ML Jr,Ball JA,Randall DW,Britt RD

更新日期：1995-10-10 00:00:00

abstract：:Friend murine leukemia virus (F-MuLV) is a replication-competent retrovirus that induces a rapidly fetal leukemia in susceptible mice (stage I disease). Leukemia cells obtained from these animals do not grow in cell culture using standard tissue culture conditions. However, in the presence of WEHI-3 cell-conditioned m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oliff A,Oliff I,Schmidt B,Famulari N

更新日期：1984-09-01 00:00:00

abstract：:We previously reported that laminar flow activates peroxisome proliferator-activated receptor gamma (PPARgamma) in vascular endothelial cells in a ligand-dependent manner that involves phospholipase A2 and cytochrome P450 epoxygenases. In this study, we investigated whether epoxyeicosatrienoic acids (EETs), the cataly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Y,Zhang Y,Schmelzer K,Lee TS,Fang X,Zhu Y,Spector AA,Gill S,Morisseau C,Hammock BD,Shyy JY

更新日期：2005-11-15 00:00:00

abstract：:The pleiotropic effect of the sickle gene suggests that factors in addition to polymerization of the mutant gene product might be involved in sickle disease pathobiology. We have examined rates of heme transfer to hemopexin from hemoglobin in dilute aqueous solution (0.5 mg of Hb per ml) at 37 degrees C. HbO2 S loses ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hebbel RP,Morgan WT,Eaton JW,Hedlund BE

更新日期：1988-01-01 00:00:00

abstract：:cDNA libraries were constructed from pathogenic (HM-1:IMSS) and nonpathogenic (SAW 1734) isolates of Entamoeba histolytica. A cDNA clone (cEH-P1) specific for pathogenic amoebae was identified by screening with a pool of sera from patients with invasive amoebiasis that had been absorbed with nonpathogenic amoebae. Thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tannich E,Horstmann RD,Knobloch J,Arnold HH

更新日期：1989-07-01 00:00:00

abstract：:Frame-shift mutations at microsatellites occur as a time-dependent function of polymerase errors followed by failure of postreplicational mismatch repair. A cell-culture system was developed that allows identification of intermediate mutant cells that carry the mutation on a single DNA strand after the initial DNA pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gasche C,Chang CL,Natarajan L,Goel A,Rhees J,Young DJ,Arnold CN,Boland CR

更新日期：2003-02-18 00:00:00

abstract：:A major challenge of cancer immunotherapy is the persistence and outgrowth of subpopulations that lose expression of the target antigen. IL-15 is a potent cytokine that can promote organ-specific autoimmunity when up-regulated on tissue cells. Here we report that T cells eradicated 2-wk-old solid tumors that expressed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu RB,Engels B,Schreiber K,Ciszewski C,Schietinger A,Schreiber H,Jabri B

更新日期：2013-05-14 00:00:00

abstract：:Allostery plays a key role in the regulation of the activity and function of many biomolecules. And although many ligands act through allostery, no systematic use is made of it in drug design strategies. Here we describe a procedure for identifying the regions of a protein that can be used to control its activity thro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laine E,Goncalves C,Karst JC,Lesnard A,Rault S,Tang WJ,Malliavin TE,Ladant D,Blondel A

更新日期：2010-06-22 00:00:00

abstract：:A mutant of E. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to replicate DNA of various sources was analyzed. Mutant bacteria grow normally, at 41 degrees as well as at 30 degrees . Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirota Y,Gefter M,Mindich L

更新日期：1972-11-01 00:00:00

abstract：:L-type Ca(2+) currents conducted by CaV1.2 channels initiate excitation-contraction coupling in the heart. Their activity is increased by β-adrenergic/cAMP signaling via phosphorylation by PKA in the fight-or-flight response, but the sites of regulation are unknown. We describe the functional role of phosphorylation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu Y,Westenbroek RE,Scheuer T,Catterall WA

更新日期：2013-11-26 00:00:00

abstract：:Protein tyrosine phosphatase (PTP)σ (PTPRS) was shown previously to be associated with susceptibility to inflammatory bowel disease (IBD). PTPσ(-/-) mice exhibit an IBD-like phenotype in the intestine and show increased susceptibility to acute models of murine colitis. However, the function of PTPσ in the intestine is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murchie R,Guo CH,Persaud A,Muise A,Rotin D

更新日期：2014-01-14 00:00:00

abstract：:How individuals make decisions has been a matter of long-standing debate among economists and researchers in the life sciences. In economics, subjects are viewed as optimal decision makers who maximize their overall reward income. This framework has been widely influential, but requires a complete knowledge of the rew...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kubanek J

更新日期：2017-08-08 00:00:00