[The extracellular ionic environment and bone metabolism. In vitro study of the role of chloride].

Abstract:

:Controversies surround the view that the blood-bone ionic equilibrium is controlled by ionic changes at a so called "bone membrane" that display a pump-leak mechanism able to maintain a concentration gradient for chloride in addition to that for calcium, sodium and potassium. On the other hand it is known that a chloride-bicarbonate transport is active at bone cells membrane to control cytosolic pH. We tested the hypothesis that a modification of the chloride concentration of the culture medium, as well as a block of the chloride-bicarbonate transport by an anionic channels blocker, in an experimental model that keeps intact the bone membrane, may influence the bone cells activity and that the influence may be quantitatively different to that previously observed in isolated bone cells. The experimental model is the intact fetal rat limb bone, cultured for 24 hours in a simplified medium at different chloride concentrations obtained by substituting chloride with isethionate. Alkaline phosphatase activity released in the medium was measured as a marker of osteoblasts activity. By progressively reducing the chloride concentration from 117 mM to 29 mM, alkaline phosphatase activity was found unchanged. By dramatically reducing the medium chloride concentration to the range 14-0 mM, alkaline phosphatase activity was significantly (p = 0.0002) inhibited by 31.58%.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Minerva Endocrinol

journal_title

Minerva endocrinologica

authors

Rubinacci A,Boniforti F,De Ponti A

keywords:

subject

Has Abstract

pub_date

1992-07-01 00:00:00

pages

95-101

issue

3

eissn

0391-1977

issn

1827-1634

journal_volume

17

pub_type

杂志文章
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    更新日期:2004-12-01 00:00:00

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    authors: Vondra K,Vrbikova J,Dvorakova K

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  • Bariatric surgery as an option for diabetes mellitus prevention and treatment in obese persons.

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  • Neuroprotection of sex steroids.

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  • Managing hyponatremia in neurosurgical patients.

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  • Clinical applications of 68Ga-DOTANOC in neuroendocrine tumours.

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    authors: Lopci E,Nanni C,Rampin L,Rubello D,Fanti S

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  • The possible impact of advanced glycation end products on pregnancy outcome in women with diabetes mellitus type 1.

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    pub_type: 杂志文章,评审

    doi:10.23736/S0391-1977.16.02477-9

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    authors: Asnani S,Desouza C,Homan J,Murthy SN,McNamara DB,Fonseca V

    更新日期:2002-09-01 00:00:00

  • Diagnosis, treatment, clinical course, and prognosis of childhood-onset craniopharyngioma patients.

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    pub_type: 杂志文章,meta分析,评审

    doi:10.23736/S0391-1977.17.02615-3

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    更新日期:2017-12-01 00:00:00

  • Effects of thyroxine withdrawal in biochemical parameters and cardiac function and structure in patients with differentiated thyroid cancer.

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    authors: Chrisoulidou A,Pazaitou-Panayiotou K,Kaprara A,Platoyiannis D,Lafaras C,Boudina M,Georgiou E,Drimonitis A,Bischiniotis T,Vainas I

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  • Pancreatitis and pancreatic cancer in patients with type 2 diabetes treated with glucagon-like peptide-1 receptor agonists: an updated meta-analysis of randomized controlled trials.

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