Tumor and normal thyroid spheroids: from tissues to zebrafish.

Abstract:

BACKGROUND:Multicellular spheroids represent an interesting experimental model with promising applications in the pre-clinical studies on anticancer drugs. We recently demonstrated that thyroid spheroids recapitulate the features of the original tissues, in either the differentiated and "stem-like" components. Here we were aimed to characterize thyroid spheroids and to investigate in vivo the proangiogenic potential of patient-derived xenografts (PDX) of spheroids obtained from papillary thyroid cancer (PTC) and the matched normal tissues. METHODS:Spheroids cultures were obtained from 11 PTCs and matched normal tissues and characterized by immunohistochemistry. The expression of p53, involved in the regulation of stem cell homeostasis, was evaluated. The proangiogenic effect of thyroid spheroids was assessed by the injection in zebrafish embryos. RESULTS:Thyroid spheroids are enriched in stem-like cells, as shown by the positivity for the stem cell marker OCT4, and by the low level of p53 expression. Interestingly, PTCs and normal thyroid tissues have a detectable p53 expression, whereas the derived spheroids are mainly constituted by cells that express p53 at a lower level. Finally, we show that PDXs derived from PTC or normal spheroids stimulate the migration and the growth of sprouting vessels toward the implant into the zebrafish embryos. CONCLUSIONS:We report the characterization of multicellular spheroids obtained from PTCs and normal thyroid tissues, showing that they are enriched in stem-like cells. Moreover, we established xenografts of spheroids in zebrafish, demonstrating that they stimulate neoangiogenesis. This in vivo model could be considered as a valuable platform to test the effects of anticancer drugs.

journal_name

Minerva Endocrinol

journal_title

Minerva endocrinologica

authors

Cirello V,Gaudenzi G,Grassi ES,Colombo C,Vicentini L,Ferrero S,Persani L,Vitale G,Fugazzola L

doi

10.23736/S0391-1977.17.02610-4

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

1-10

issue

1

eissn

0391-1977

issn

1827-1634

pii

S0391-1977.17.02610-4

journal_volume

43

pub_type

杂志文章
  • Thyroid gland diseases in adult patients with diabetes mellitus.

    abstract::This review concerns the relation between most frequent thyroid gland diseases and diabetes mellitus in adult patients. Special attention is paid to autoimmune thyroiditis, Graves' disease, thyroid autoimmunity in pregnant diabetic women, and iodine metabolism. We focused on mechanisms leading to coexistence of both e...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Vondra K,Vrbikova J,Dvorakova K

    更新日期:2005-12-01 00:00:00

  • Neuroendocrine tumors of the gastroenteric canal. Anatomopathological and diagnostic-therapeutic definition. Description of a case with a rare cecal localisation.

    abstract::The authors take the case of a neuroendocrine tumour of the cecum as the starting point for an analysis of the anatomopathological and diagnostic-therapeutic aspects of these neoplasms. Furthermore, the authors underline that neuroendocrine tumours (NET) of the colon represent an extremely rare nosological entity and ...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: Bonomi M,De Cesare A,Atella F,Angelini M,Graziano P,Piat G

    更新日期:1999-09-01 00:00:00

  • Role of the IGF axis in prostate cancer.

    abstract::The major issue currently being faced in the management of prostate cancer is the inability to distinguish between indolent prostate tumors that will not present clinically from more aggressive and metastatic prostate cancers that will impact on men's lives. Only a small proportion of prostate cancers can be accounted...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Biernacka KM,Perks CM,Holly JM

    更新日期:2012-06-01 00:00:00

  • [Unusual endocrine behavior in adrenal carcinoma. A case study].

    abstract::The Authors report a case of left adrenal mass incidentally discovered by upper abdominal echogram in a 40 year old man. Physical examination showed no signs of hypercortisolism and plasma cortisol and ACTH levels were in the normal range as well as urinary free cortisol. After adrenalectomy, 4 and 8 month follow-up w...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: Zingrillo M,Errico M,D'Aloiso L,Torlontano M,Parracino T,Gomes V

    更新日期:1991-10-01 00:00:00

  • Therapeutic strategy of papillary microcarcinoma of the thyroid gland: a nuclear medicine perspective.

    abstract::According to the literature, the prevalence of papillary thyroid microcarcinoma (PTMC) is increasing. To date, PMTC account for up to 30% of all differentiated thyroid cancers. Patients with PTMC have an excellent prognosis with a normal life expectancy. Because of the differential definitions of the PTMC, the therape...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Riemann B,Schober O

    更新日期:2009-03-01 00:00:00

  • Measuring ovarian function in young cancer survivors.

    abstract::It is known that young female cancer survivors are at higher risk for decreased fertility and early menopause. Because the risk of these outcomes varies by age, cancer type, treatment regimen and other patient-specific characteristics, there is a need for valid tools for measuring and predicting reproductive function ...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Su HI

    更新日期:2010-12-01 00:00:00

  • The beta-cell death in long-term type 2 diabetes mellitus: never say never again.

    abstract:: ...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:10.23736/S0391-1977.18.02852-3

    authors: Koufakis T,Ioannidis I,Zebekakis P,Kotsa K

    更新日期:2019-03-01 00:00:00

  • Hormones and homocysteine.

    abstract::Hyperhomocysteinemia is a well-established risk factor for cardiovascular disease. Various factors, both modifiable and non-modifiable, interact with the homocysteine metabolism and determine the plasma homocysteine concentrations. These include genetic abnormalities, age, sex and various nutritional and hormonal dete...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Asnani S,Desouza C,Homan J,Murthy SN,McNamara DB,Fonseca V

    更新日期:2002-09-01 00:00:00

  • Surgical therapeutic planning options in nodular goiter.

    abstract::Nodular goiter encompasses a spectrum of diseases from the incidental asyntomatic small solitary nodule to the large intrathoracic goiter causing pressure symptoms as well as functional complaints. The mainstay in the diagnostic evaluation is related to functional and morphological characterization with serum thyroid-...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Pelizzo MR,Merante Boschin I,Toniato A,Sorgato N,Marzola MC,Rubello D

    更新日期:2010-09-01 00:00:00

  • Subclinical hypothyroidism.

    abstract::Subclinical hypothyroidism - i.e., a condition characterized by serum TSH concentrations above the normal reference range in the presence of normal serum T4 levels - affects 4 to 20% of the population living in iodine-sufficient areas. The present work reviews the clinical challenges regarding, on the one hand, the si...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:10.23736/S0391-1977.20.03176-4

    authors: Chbat J,Ezzat S,Papi G

    更新日期:2020-08-03 00:00:00

  • [Association of hyperthyroidism and diabetes mellitus. Description of a case with partial recovery of pancreatic beta-islet function].

    abstract::The coexistence of Graves' disease and insulin-dependent diabetes mellitus is well known among autoimmune polyglandular syndromes and sustained by common underlying immune pathogenic factors. Hyperthyroidism itself may lead to impaired glucose tolerance in subjects with intact beta-cell function through various not we...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: Pandolfi C,Pellegrini L,Sbalzarini G

    更新日期:1992-04-01 00:00:00

  • Growth hormone-releasing hormone and pituitary somatotrope proliferation.

    abstract::Growth hormone-releasing hormone (GHRH) is a hypothalamic hormone that is essential for normal expansion of the somatotrope lineage during pituitary development. Decreased GHRH secretion and/or action leads to impairment of this process and somatotrope hypoplasia in both humans and experimental animals. Excessive GHRH...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Frohman LA,Kineman RD

    更新日期:2002-12-01 00:00:00

  • Mediterranean diet and breast cancer risk: a narrative review.

    abstract::Breast cancer is the second most frequent type of cancer worldwide and the most commonly occurring malignancy in women, and its incidence is increasing in most developed and developing countries. There is growing evidence that lifestyle factors, in particular diet may be associated with higher Breast Cancer risk. Some...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:10.23736/S0391-1977.20.03266-6

    authors: Laudisio D,Castellucci B,Barrea L,Pugliese G,Savastano S,Colao A,Muscogiuri G

    更新日期:2020-09-24 00:00:00

  • Diabetes and the hypothalamo-pituitary-adrenal (HPA) axis.

    abstract::Patients and animals with poorly controlled or uncontrolled diabetes present with diurnal hypersecretion of glucocorticoids and altered regulation of the hypothalamo-pituitary-adrenocortical (HPA) axis. Although some of these changes are reversed with insulin replacement therapy, neuroendocrine function is not always ...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Chan O,Inouye K,Riddell MC,Vranic M,Matthews SG

    更新日期:2003-06-01 00:00:00

  • Very-low-calorie ketogenic diet for the management of obesity, overweight and related disorders.

    abstract::Very-low-calorie ketogenic diet (VLCKD) is a promising lifestyle intervention for the management of overweight and obesity. It is characterized by a restriction of both calories and carbohydrates, while assuring the adequate proteins, fats and micronutrients intake. Significant results in terms of excess body weight l...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:10.23736/S0391-1977.20.03356-8

    authors: Castellana M,Biacchi E,Procino F,Casanueva FF,Trimboli P

    更新日期:2020-11-19 00:00:00

  • [Fetal macrosomia in children of diabetic mothers: relationship with excess weight in early years of life].

    abstract::One hundred children (4.1 +/- 1.9 years) were examined: 31 of type I diabetic mothers, 25 of type II diabetic mothers and 44 of gestational diabetic mothers. The fetal body weight index at delivery was determined according to Babson. The weight/height index was determined according to Tunner's Centiles, when the child...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: Botta RM,Salvo C,Galluzzo G

    更新日期:1990-07-01 00:00:00

  • [New developments in the treatment of medullary thyroid carcinoma].

    abstract::Medullary thyroid carcinoma arises from the C cells, which produce a characteristic hormone, calcitonin. At present, surgery is the main treatment modality. Medullary thyroid carcinoma is usually treated with total thyroidectomy and with removal of nodes in the central portion of the neck and upper mediastinum. Cervic...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Vitale G,Cascone E,Lupoli G Jr,Ciccarelli A,Barone C,Lupoli G

    更新日期:1998-12-01 00:00:00

  • Optimizing therapy for radioactive iodine-refractory differentiated thyroid cancer: current state of the art and future directions.

    abstract::The majority of patients with differentiated thyroid cancer are cured with standard primary treatments including surgery, radioactive iodine and TSH suppression. A small proportion of patients who develop radioactive iodine-refractory metastatic disease have few treatment options. Recent discovery of the molecular mec...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Dadu R,Cabanillas ME

    更新日期:2012-12-01 00:00:00

  • [The thyroid nodule. Diagnostic considerations].

    abstract::Thyroid nodule is extremely common. Its prevalence has been estimated to be more than 5% in the adult population. If thyroid nodule is a rare condition, 0.004% a year, identifying those nodules, which are likely to be malignant, is very important for physicians. The evaluation of thyroid is carried out using the fine ...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Danese D,Sciacchitano S

    更新日期:1993-09-01 00:00:00

  • [Applications and results of photon absorptiometry].

    abstract::Bone mineral content (CMO, mg/cm2) was measured at the distal radius by dual photon absorptiometry (DPA, I125, Am241) in 1.161 women (w), aged 20-87 years. Women were subdivided into different groups according to the presence and duration of menopause. For gross evaluation of spinal osteoporosis (OP), the results of s...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: Di Munno O,Occhipinti G,Pontrandolfo A

    更新日期:1991-04-01 00:00:00

  • [Hypopituitarism secondary to suprasellar giant carotido-ophthalmic aneurysm. Normalization of the hypophyseal function after neurosurgical depression of the aneurysm].

    abstract::A 49-year-old man presented with a 6-month history of weight loss, muscular weakness, easy fatigue, impotence, decreased visual acuity, campimetry defects. The results of radiologic and endocrine testing disclosed the presence of pituitary dysfunction due to pituitary stalk section caused by a giant suprasellar aneury...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: Giustina A,Scalvini T,Cerudelli B,Bossoni S,Bodini C,Orlandini A,Romanelli G

    更新日期:1989-10-01 00:00:00

  • Aromatase and prostate cancer.

    abstract::The normal growth and development of the prostate requires the presence and action of androgens, which are also known risk factors in the origins of benign and malignant prostate disease. Paradoxically, the incidence of prostate disease increases with age when serum androgen levels are in decline and emerging evidence...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Ellem SJ,Risbridger GP

    更新日期:2006-03-01 00:00:00

  • Thyroid nodules and Doppler ultrasonography. A new element for an old puzzle?

    abstract:BACKGROUND:Whether Doppler ultrasonography (DU) may be of help in characterizing thyroid nodes is still under debate: both good and unsatisfactory results have been claimed, either in evaluating the presence of malignancy or in identifying hyperfunctional structures. METHODS:In this paper, the scintigraphic and Dopple...

    journal_title:Minerva endocrinologica

    pub_type: 临床试验,杂志文章

    doi:

    authors: Lupi A,Cerisara D,Orsolon P,De Antoni G,Vianello Dri A

    更新日期:1999-03-01 00:00:00

  • [Cardiovascular response to the cold test in obese subjects. Effect of a hypocaloric, normal sodium diet].

    abstract::Loss of weight in obese patients, both hypertensive and normotensive, causes a fall in blood pressure (BP) through a mechanism which is still not fully understood. The effects of a low-sodium low-energy diet on BP were assessed in 20 obese subjects (15 M and 5 F; age 26-65 years), 11 of whom were normotensive and 9 hy...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: De Simone G,Mancini M,Turco S,Marotta T,Gaeta I,Iannuzzi R,Ferrara LA,Mancini M

    更新日期:1990-10-01 00:00:00

  • [Insulin resistance in obese and non-obese patients with polycystic ovary syndrome. Possible role in the pathogenesis of the disease].

    abstract::The pathogenesis of Polycystic Ovary Syndrome (PCOs) is not well known till now. Previous reports indicated an hyperinsulinemia and insulin resistance in obese and non obese PCOs. However the role of hyperinsulinemia in PCOs pathogenesis is not completely understood. In this study we evaluated the glycemic and insulin...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: Vagiri D,Barletta C,Cioli I,Scavo D

    更新日期:1994-03-01 00:00:00

  • Adrenal disorders and non-alcoholic fatty liver disease.

    abstract::Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease in the developed world and its pathogenesis is complex and multifactorial. It is considered the hepatic manifestation of the metabolic syndrome and is the leading cause of hepatic cirrhosis. This review aims to present current knowledge on the ...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:10.23736/S0391-1977.16.02583-9

    authors: Papanastasiou L,Fountoulakis S,Vatalas IA

    更新日期:2017-06-01 00:00:00

  • [Percutaneous ethanol injections in the treatment of autonomous thyroid nodules. 4 years' experience].

    abstract::We treated with PEI 81 patients carriers of autonomous thyroid nodule, 65 toxic and 16 non toxic, all with undetectable serum TSH and suppressed extranodular tissue on scintigraphy. The treatment schedule was: 1-2 sessions per week, 1-8 ml per session, 3-13 sessions in total, In relation to the size and the therapeuti...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:

    authors: Livraghi T,Ferrari C,Paracchi A,Reschini E,Macchi RM,Ciocia GL,Pirola P

    更新日期:1993-12-01 00:00:00

  • The possible role of endocrine dysfunction of adipose tissue in gestational diabetes mellitus.

    abstract::Gestational diabetes mellitus (GDM) is diabetes that is first diagnosed in the second or third trimester of pregnancy in patients who did not have a history of diabetes before pregnancy. Consequences of GDM include increased risk of macrosomia and birth complications in the infant and an increased risk of maternal typ...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章

    doi:10.23736/S0391-1977.20.03192-2

    authors: Šimják P,Anderlová K,Cinkajzlová A,Pařízek A,Kršek M,Haluzík M

    更新日期:2020-09-01 00:00:00

  • The cell cycle in steroid hormone regulated proliferation and differentiation.

    abstract::Steroid hormones mediate pleiotropic cellular processes involved in metabolism, cellular proliferation, and differentiation. The ability of the cell to respond to its hormonal environment is transduced by nuclear receptors (NRs) that bind both hormone and DNA. Hence, NRs represent a link between the external hormonal ...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Amanatullah DF,Zafonte BT,Pestell RG

    更新日期:2002-03-01 00:00:00

  • Bariatric surgery as an option for diabetes mellitus prevention and treatment in obese persons.

    abstract::Bariatric surgery has been found to be very useful in treating established type-2 diabetes in obese persons, as well as in borderline diabetics, besides giving a good weight loss. Various modifications of the primary bariatric procedures have been introduced, with differ advantages and better efficacy. Good incretin r...

    journal_title:Minerva endocrinologica

    pub_type: 杂志文章,评审

    doi:

    authors: Ugale S,Agarwal D,Satwalekar V,Rao N,Ugale A

    更新日期:2016-12-01 00:00:00