Abstract:
:Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current methods of antibody production in animal systems. In this study, the heavy and light chains of human anti-rabies mAb were expressed and assembled in planta under the control of two strong constitutive promoters. An alfalfa mosaic virus untranslated leader sequence and Lys-Asp-Glu-Leu (KDEL) endoplasmic reticulum retention signal were linked at the N and C terminus of the heavy chain, respectively. mAbP was as effective at neutralizing the activity of the rabies virus as the mammalian-derived antibody (mAbM) or human rabies Ig (HRIG). The mAbP contained mainly oligomannose type N-glycans (90%) and had no potentially antigenic alpha(1,3)-linked fucose residues. mAbP had a shorter half-life than mAbM. The mAbP was as efficient as HRIG for post-exposure prophylaxis against rabies virus in hamsters, indicating that differences in N-glycosylation do not affect the efficacy of the antibody in this model.
journal_name
Proc Natl Acad Sci U S Aauthors
Ko K,Tekoah Y,Rudd PM,Harvey DJ,Dwek RA,Spitsin S,Hanlon CA,Rupprecht C,Dietzschold B,Golovkin M,Koprowski Hdoi
10.1073/pnas.0832472100keywords:
subject
Has Abstractpub_date
2003-06-24 00:00:00pages
8013-8issue
13eissn
0027-8424issn
1091-6490pii
0832472100journal_volume
100pub_type
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