Abstract:
:Dendritic cells play a major role in cellular immunity. The crucial steps of antigen presentation and processing by DCs may be limiting factors for adoptive cellular immunotherapy. Here, we investigated whether hyperthermia of human hepatocellular carcinoma (HCC) cells induces enhanced cytotoxic cellular immune response. Peripheral blood mononuclear cell (PBMC)-derived DCs were pulsed with tumor cell lysate of the human HCC cell line HepG2, which had been heat shocked prior to incubation for 5 h. Subsequent to TNFalpha-induced maturation DCs were co-cultured with autologous CD4+ and/or CD8+ cells, and T cell mediated cytolysis of HepG2 cells was assessed. We observed enhanced CD4+/8+ cellular cytotoxicity against HepG2 cells subsequent to co-culture with the heat shocked tumor lysate pulsed DCs as compared to pulsing DCs with lysate of non-heat shocked tumor cells. The improved cellular immune response can be related to enhanced expression of HSP 70 and 90 in HepG2 cells upon hyperthermia.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Schueller G,Stift A,Friedl J,Dubsky P,Bachleitner-Hofmann T,Benkoe T,Jakesz R,Gnant Mkeywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
1397-402issue
6eissn
1019-6439issn
1791-2423journal_volume
22pub_type
杂志文章abstract::The lack of good molecular markers for diagnosis as well as treatment assessment has rendered the hepatocellular carcinoma (HCC) a major challenge in health care. In this study, woodchucks were used as an animal model for hepatitis virus-induced HCC, and gene expression studies were performed using a human oligonucleo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Marek's disease virus (MDV) is one of the most oncogenic herpesviruses and the only cancer virus that has a successful vaccine. Based on the short-latency and polyclonal nature of the tumors, it is likely that MDV encodes its own oncogenes. Recent studies have provided insights into possible MDV genes involved in onco...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.5.997
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abstract::We established new two protein-free culture subclones from murine well-characterized Ehrlich ascites carcinoma and P815 mastocytoma using intermittent protein-free culture performed previously for a protein-free subclone of fibrosarcoma (Gc-4 PF). The Ehrlich protein-free subclone (Ehrlich PF) grew much more slowly th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.1.4.481
更新日期:1992-09-01 00:00:00
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pub_type: 杂志文章
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abstract::Seaweeds are commonly used as functional foods and drugs. A glycoprotein (GP) from the green alga Capsosiphon fulvescens (Cf) has been reported to have antitumor activity toward various cancer cells. We previously observed that Cf-GP induced different pathways of apoptosis in AGS human gastric cancer cells. Transformi...
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更新日期:2013-10-01 00:00:00
abstract::Newcastle disease virus (NDV) is an avian paramyxovirus with oncolytic properties which shows promising effects in the treatment of cancer. Anti-cancer effects are due to the virus ability: i) to replicate in and kill tumor cells, leading finally to their selective elimination; and ...
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abstract::Collagen type I is an abundant component of the extracellular matrix and due to its longevity, constitutes a prominent target of non-enzymatic post-translational in vivo modifications such as carbamylation and glycation. These protein modifications involved in aging, renal diseases and diabetes, are linked to elevated...
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abstract::DNA replication is a vital process in cell division where anomalies can lead to tumorigenesis. Minichromosome maintenance complex component 10 (MCM10) plays a crucial role in this process. However, the role of MCM10 in lung cancer pathogenesis remains to be elucidated. In current study, using the publicly available lu...
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abstract::Human telomerase is a ribonucleoprotein DNA polymerase which maintains the telomeric region of human chromosomes and has been detected in all types of human cancer tested. We used the telomeric repeat amplification protocol (TRAP) assay to examine 71 non-small cell lung carcinomas (NSCLC) and their adjacent normal tis...
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pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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pub_type: 杂志文章
doi:10.3892/ijo.2013.1774
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.6.1279
更新日期:1994-12-01 00:00:00
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journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::We included in a case-control study on brain tumours and mobile and cordless telephones 1,617 patients aged 20-80 years of both sexes diagnosed during January 1, 1997 to June 30, 2000. They were alive at the study time and had histopathology verified brain tumour. One matched control to each case was selected from the...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-02-01 00:00:00
abstract::MicroRNAs can govern up to hundred different mRNAs and are important regulators of gene expression programs in development and disease. We analyzed the expression of microRNA-21, one of the most common oncomirs, in non-small cell lung cancer (NSCLC). Using northern blots the microRNA-21 expression levels of NSCLC-deri...
journal_title:International journal of oncology
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doi:10.3892/ijo.2014.2272
更新日期:2014-04-01 00:00:00
abstract::This study evaluated the effects of a combination of opioid growth factor (OGF) and paclitaxel on squamous cell carcinoma of the head and neck (SCCHN) using a tissue culture model of human SCCHN. The combination of OGF and paclitaxel was markedly inhibitory to SCCHN proliferation, reducing growth from control levels b...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract::One of the genes that the authors have isolated by a differential display of hepatocellular carcinoma compared to adjacent liver is the alpha6 integrin. alpha6 integrin is the major adhesion receptor for laminin and is suggested to be involved in tumor cell invasion and metastasis. To our knowledge, however, there are...
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journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,随机对照试验
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abstract::We have previously isolated a drug-resistant, [PC3(R)], variant of human prostate PC3 cell line, which showed significant resistance (>10-fold) to adriamycin. No known mechanisms of drug resistance were found; however, resistant cells expressed more bcl2, c-myc, and ms oncogenes compared to the sensitive cells. In thi...
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更新日期:1997-10-01 00:00:00
abstract::It is well established that the tumor microenvironment plays a major role in the aggressive behavior of malignant solid tumors. Among cell types associated with tumor microenvironment, tumor-associated macrophages (TAMs) are the most influential for tumor progression. Breast cancer is characterized by having a large p...
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更新日期:2007-03-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
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