Abstract:
:We have previously isolated a drug-resistant, [PC3(R)], variant of human prostate PC3 cell line, which showed significant resistance (>10-fold) to adriamycin. No known mechanisms of drug resistance were found; however, resistant cells expressed more bcl2, c-myc, and ms oncogenes compared to the sensitive cells. In this study, we found that buthionine sulfoximine (BSO), an inhibitor of gamma-glutamyl-cysteine synthetase, decreased glutathione levels by 80-90% in both cell lines. BSO treatment down-modulated Ras protein only in PC3(R) cells and caused a 4-fold sensitization of PC3(R) cells to adriamycin without affecting PC3(W) cells. Farnesol treatment also inhibited expression of Ras protein and concomitantly reversed adriamycin resistance in PC3(R) cells, indicating that altered levels of ras expression plays an important role in drug resistance in PC3(R) cells.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Sinha B,Song Kdoi
10.3892/ijo.11.4.819subject
Has Abstractpub_date
1997-10-01 00:00:00pages
819-23issue
4eissn
1019-6439issn
1791-2423journal_volume
11pub_type
杂志文章abstract::Although several therapeutic options are available for hepatocellular carcinoma (HCC), the outcome is still very poor. One reason is the complexity of signal transduction in the pathogenesis of HCC. The aim of this study was to identify new HCC-related genes and to investigate the functions of these genes in the patho...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2445
更新日期:2014-08-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) poses a significant challenge clinically where one of the mechanisms responsible for the invasion into facial bones occurs via the activation of osteoclasts. Copper has been demonstrated to play a key role in skeletal remodeling. However, the role of copper in cancer-assoc...
journal_title:International journal of oncology
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更新日期:2018-03-01 00:00:00
abstract::Bile acids serve a critical role in the induction of gastric intestinal metaplasia (IM) and gastric carcinogenesis. The present study investigated the effects of bile acids on the induction of gastric IM formation. The results demonstrated that the expression levels of caudal‑related homeobox transcription factor 2 (C...
journal_title:International journal of oncology
pub_type: 杂志文章
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abstract::A multi stage extraction procedure which gives the possibility to analyze both water soluble and lipid components stemming from the same specimen has been developed in our laboratory. Metabolites from brain cancer biopsies have been compared to metabolites in normal human brain, developing mouse brain and primary mous...
journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
abstract::Malignant astrocytomas are highly invasive, vascular neoplasms that comprise the majority of nervous system tumors in humans. A strong association has previously been made between malignancy in human astrocytic tumors and increased expression of certain fibroblast growth factor (FGF) family members. MG-160 is an intri...
journal_title:International journal of oncology
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更新日期:2003-05-01 00:00:00
abstract::Carnosic acid is a phenolic diterpene with anti-inflammation, anticancer, anti-bacterial, anti-diabetic, as well as neuroprotective properties, which is generated by many species from Lamiaceae family. Fisetin (3,3',4',7-tetrahydroxyflavone), a naturally flavonoid is abundantly produced in different vegetables and fru...
journal_title:International journal of oncology
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更新日期:2017-06-01 00:00:00
abstract::Retroviral infection of hTERT, the catalytic component of telomerase, into BJ fibroblasts (population doubling 28) resulted in reconstitution of telomerase activity, telomere maintenance, and extension of in vitro lifespan. The hTERT-infected cells also exhibited increased growth rate and colony forming efficiency rel...
journal_title:International journal of oncology
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更新日期:2002-06-01 00:00:00
abstract::The ability of lonidamine (LND), an energolytic derivative of indazol-carboxylic acid, to modulate the cytotoxic activity of idarubicin (IDA) and doxorubicin (DX) was investigated in two human lymphoma cell lines (H9 and U937). A different pattern of interaction between the drugs was observed as a function of treatmen...
journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
abstract::Recent studies have reported that chemically synthesized small activating RNA (saRNA) targeting the promoter regions of a gene can activate its expression in different cell lines. This technique can be a powerful therapeutic method for diseases caused by complete inactivation or reduced expression of specific genes. E...
journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
abstract::The enzyme poly(ADP-ribose)polymerase (PARP) has been implicated in several cellular processes by the demonstration of ADP-ribosylation of proteins which play a role in cellular repair of DNA damage, response to ionizing radiation and apoptosis. In addition, ADP-ribosylation has been shown to play a role in cell cycle...
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abstract::Serial analysis of gene expression (SAGE) is a sequence-based tool that enables quantitative study of comprehensive gene expression profiles. In this study, we generated a SAGE library of hepatitis B virus (HBV)-associated small hepatocellular carcinoma (HCC) and compared it with libraries of normal liver and other or...
journal_title:International journal of oncology
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更新日期:2009-07-01 00:00:00
abstract::The representative halophyte Calystegia soldanella (L) Roem. et Schult is a perennial vine herb that grows in coastal dunes throughout South Korea as well as in other regions around the world. This plant has long been used as an edible and medicinal herb to cure rheumatic arthritis, sore throat, dropsy, and scurvy. So...
journal_title:International journal of oncology
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abstract::Patients with long-standing ulcerative colitis (UC) have higher risk of developing colorectal cancer. Albeit the causes remain to be understood, epigenetic alterations have been suggested to play a role in the long-term cancer risk of these patients. In this work, we developed a novel microarray platform based on meth...
journal_title:International journal of oncology
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journal_title:International journal of oncology
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更新日期:2018-05-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2182
更新日期:2014-01-01 00:00:00
abstract::We examined the expression levels of P-glycoprotein (P-Gp)/the human multidrug resistance gene (MDR1) and in vivo chemosensitivity in the 7 osteosarcoma xenografts. Three of seven (43%) osteosarcoma xenografts expressed MDR1 by reverse transcriptase-polymerase chain reaction (RT-PCR). The OSS-516R xenograft selected w...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Recently, a novel candidate tumor suppressor gene, DPC 4/SMAD 4, has been implicated in the development of pancreatic cancers. Its location at human chromosome 18q21 prompted us to investigate this gene in a large series of primary tumors located outside the gastrointestinal tract which have been associated with loss ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Hepatitis B X-interacting protein (HBXIP) is a novel oncoprotein. We have previously reported that HBXIP promotes the proliferation and migration of breast cancer cells. S-phase kinase-associated protein 2 (Skp2) is another oncoprotein which is important for migration. In this study, we investigated whether Skp2 is in...
journal_title:International journal of oncology
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abstract::The p53-induced wig-1 gene encodes an unusual nuclear zinc finger protein with high sequence similarity between human, rat and mouse. Wig-1 belongs to a group of proteins with widely spaced zinc fingers that bind double stranded (ds) RNA. We show here that wig-1 is present in Gallus gallus (chicken), Silurana tropical...
journal_title:International journal of oncology
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journal_title:International journal of oncology
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doi:
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abstract::MicroRNAs can govern up to hundred different mRNAs and are important regulators of gene expression programs in development and disease. We analyzed the expression of microRNA-21, one of the most common oncomirs, in non-small cell lung cancer (NSCLC). Using northern blots the microRNA-21 expression levels of NSCLC-deri...
journal_title:International journal of oncology
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abstract::We analyzed the representation of CD44 isoforms with both exons v9 and v10 among CD44 total amount and also examined correlation between their expression, clinical parameters and survival. We used a semi-quantitative RT-PCR reaction and a panel of 25 normal breast specimens, 10 mammary fibroadenomas, 8 cystic samples ...
journal_title:International journal of oncology
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更新日期:1997-01-01 00:00:00
abstract::Tumour cell surfaces possess a cell surface protease (GB), which can be recognised by a cytoplasmic inhibitor protein prepared from cultured tumour cells. This enzyme inhibitor complex has been shown to be reversibly dissociated by 10-(4)M sodium dodecyl sulphate. Sections of frozen tumour tissue were used to provide ...
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journal_title:International journal of oncology
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abstract::Liposarcoma (LPS) is one of the most frequently reported type of soft‑tissue sarcoma (STS). Well‑differentiated (WD) LPS and dedifferentiated (DD) LPS are the two most common subtypes. Chemotherapy has been considered to be ineffective in LPS, and novel treatment agents are thus necessary. In this study, we reanalyzed...
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journal_title:International journal of oncology
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