Abstract:
:We investigated the effect of actin filament barbed end uncapping on Arp23 complex function both in vivo and in vitro. Arp23 complex redistributes rapidly and uniformly to the lamellar edge of activated wild-type platelets and fibroblasts but clusters in marginal actin filament clumps in gelsolin-null cells. Treatment of gelsolin-null platelets with the negative dominant N-WASp C-terminal CA domain has no effect on their residual actin nucleation activity, placing gelsolin actin filament severing, capping, and uncapping function upstream of Arp23 complex nucleation. Actin filaments capped by gelsolin or the gelsolin homolog CapG fail to enhance Arp23 complex nucleation in vitro, but uncapping of the barbed ends of these actin filaments restores their ability to potentiate Arp23 complex nucleation. We conclude that Arp23 complex contribution to actin filament nucleation in platelets and fibroblasts importantly requires free barbed ends generated by severing and uncapping.
journal_name
Proc Natl Acad Sci U S Aauthors
Falet H,Hoffmeister KM,Neujahr R,Italiano JE Jr,Stossel TP,Southwick FS,Hartwig JHdoi
10.1073/pnas.222652499keywords:
subject
Has Abstractpub_date
2002-12-24 00:00:00pages
16782-7issue
26eissn
0027-8424issn
1091-6490pii
222652499journal_volume
99pub_type
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