Maintenance by glycyl-L-glutamine in vivo of molecular forms of acetylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat.

abstract：:Many cellular cargoes move bidirectionally along microtubules, driven by teams of plus- and minus-end-directed motor proteins. To probe the forces exerted on cargoes during intracellular transport, we examined latex beads phagocytosed into living mammalian macrophages. These latex bead compartments (LBCs) are encased ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hendricks AG,Holzbaur EL,Goldman YE

更新日期：2012-11-06 00:00:00

abstract：:Cytochrome c maturation in Escherichia coli requires the ccm operon, which encodes eight membrane proteins (CcmABCDEFGH). CcmE is a periplasmic heme chaperone that binds heme covalently and transfers it onto apocytochrome c in the presence of CcmF, CcmG, and CcmH. In this work we addressed the functions of the ccmABCD...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz H,Fabianek RA,Pellicioli EC,Hennecke H,Thöny-Meyer L

更新日期：1999-05-25 00:00:00

abstract：:Antibodies to chromatin proteins of Novikoff hepatoma cells formed precipitin bands in the double-diffusion immunoprecipitation assay with chromatin proteins of Novikoff hepatoma, Walker 256 carcinosarcoma, and 18-day fetal rat liver. The antigen used for preparation of antiserum was the chromatin proteins initially e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeoman LC,Jordan JJ,Busch RK,Taylor CW,Savage HE,Busch H

更新日期：1976-09-01 00:00:00

abstract：:The frequency of recombination between the G gamma-globin (HBG2) and parathyroid hormone (PTH) loci on the short arm of human chromosome 11 was estimated by typing greater than 700 single-sperm samples from two males. The sperm-typing technique employed involves the polymerase chain reaction and allele-specific oligon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cui XF,Li HH,Goradia TM,Lange K,Kazazian HH Jr,Galas D,Arnheim N

更新日期：1989-12-01 00:00:00

abstract：:Nuclease resistance and RNA affinity are key criteria in the search for optimal antisense nucleic acid modifications, but the origins of the various levels of resistance to nuclease degradation conferred by chemical modification of DNA and RNA are currently not understood. The 2'-O-aminopropyl (AP)-RNA modification di...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teplova M,Wallace ST,Tereshko V,Minasov G,Symons AM,Cook PD,Manoharan M,Egli M

更新日期：1999-12-07 00:00:00

abstract：:The best-studied cytoskeletal system is the inner surface of the erythrocyte membrane, which provides an erythrocyte with the structural support needed to be stable yet flexible as it passes through the circulation. Current structural models predict that the spectrin-actin-based cytoskeletal network is attached to the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stefanovic M,Markham NO,Parry EM,Garrett-Beal LJ,Cline AP,Gallagher PG,Low PS,Bodine DM

更新日期：2007-08-28 00:00:00

abstract：:Mutational activation of the Ras oncogene products (H-Ras, K-Ras, and N-Ras) is frequently observed in human cancers, making them promising anticancer drug targets. Nonetheless, no effective strategy has been available for the development of Ras inhibitors, partly owing to the absence of well-defined surface pockets s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shima F,Yoshikawa Y,Ye M,Araki M,Matsumoto S,Liao J,Hu L,Sugimoto T,Ijiri Y,Takeda A,Nishiyama Y,Sato C,Muraoka S,Tamura A,Osoda T,Tsuda K,Miyakawa T,Fukunishi H,Shimada J,Kumasaka T,Yamamoto M,Kataoka T

更新日期：2013-05-14 00:00:00

abstract：:A patterning method termed "RIPPLE" (reactive interface patterning promoted by lithographic electrochemistry) is applied to the fabrication of arrays of dielectric and metallic optical elements. This method uses cyclic voltammetry to impart patterns onto the working electrode of a standard three-electrode electrochemi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kempa TJ,Bediako DK,Kim SK,Park HG,Nocera DG

更新日期：2015-04-28 00:00:00

abstract：:Understanding the approach to faulting in continental rocks is critical for identifying processes leading to fracturing in geomaterials and the preparation process of large earthquakes. In situ dynamic X-ray imaging and digital volume correlation analysis of a crystalline rock core, under a constant confining pressure...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renard F,McBeck J,Kandula N,Cordonnier B,Meakin P,Ben-Zion Y

更新日期：2019-08-13 00:00:00

abstract：:Capsaicin is a unique alkaloid of the plant kingdom restricted to the genus Capsicum. Capsaicin is the pungency factor, a bioactive molecule of food and of medicinal importance. Capsaicin is useful as a counterirritant, antiarthritic, analgesic, antioxidant, and anticancer agent. Capsaicin biosynthesis involves conden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prasad BC,Kumar V,Gururaj HB,Parimalan R,Giridhar P,Ravishankar GA

更新日期：2006-09-05 00:00:00

abstract：:Improving global yields of important agricultural crops is a complex challenge. Enhancing yield and resource use by engineering improvements to photosynthetic carbon assimilation is one potential solution. During the last 40 million years C(4) photosynthesis has evolved multiple times, enabling plants to evade the cat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whitney SM,Sharwood RE,Orr D,White SJ,Alonso H,Galmés J

更新日期：2011-08-30 00:00:00

abstract：:Regulation of the sterol-synthesizing mevalonate pathway occurs in part through feedback-regulated endoplasmic reticulum degradation of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R). In yeast, the Hmg2p isozyme of HMG-R is regulated in this manner. We have tested the involvement of ubiquitination in the regulated d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hampton RY,Bhakta H

更新日期：1997-11-25 00:00:00

abstract：:Accumulating evidence suggests that subcutaneous and visceral adipose tissues are differentially associated with metabolic disorders. In obesity, subcutaneous adipose tissue is beneficial for metabolic homeostasis because of repressed inflammation. However, the underlying mechanism remains unclear. Here, we demonstrat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang I,Jo K,Shin KC,Kim JI,Ji Y,Park YJ,Park J,Jeon YG,Ka S,Suk S,Noh HL,Choe SS,Alfadda AA,Kim JK,Kim S,Kim JB

更新日期：2019-06-11 00:00:00

abstract：:A cDNA containing the full coding region of human cytochrome b5 was inserted into a vaccinia virus cDNA expression vector. Infection of human thymidine kinase-minus (TK-) 143 cells in culture with this recombinant virus resulted in production of 0.3 nmol of cytochrome b5 per mg of cell lysate protein. The expressed cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aoyama T,Nagata K,Yamazoe Y,Kato R,Matsunaga E,Gelboin HV,Gonzalez FJ

更新日期：1990-07-01 00:00:00

abstract：:The ATM gene is mutated in the syndrome of ataxia telangiectasia (AT), associated with neurologic dysfunction, growth abnormalities, and extreme radiosensitivity. Insulin-like growth factor-I receptor (IGF-IR) is a cell surface receptor with tyrosine kinase activity that can mediate mitogenesis, cell transformation, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peretz S,Jensen R,Baserga R,Glazer PM

更新日期：2001-02-13 00:00:00

abstract：:Most Escherichia coli promoters initiate transcription with a purine 7 or 8 nt downstream from the -10 hexamer, but some promoters, including the ribosomal RNA promoter rrnB P1, start 9 nt from the -10 element. We identified promoter and RNA polymerase determinants of this noncanonical rrnB P1 start site using biochem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Winkelman JT,Chandrangsu P,Ross W,Gourse RL

更新日期：2016-03-29 00:00:00

abstract：:The paradigm of bacterial quorum sensing (QS), which mediates cell-density-dependent gene expression, usually has been studied in high-cell-density planktonic liquid cultures or in biofilms in which signal concentrations accumulate to sufficiently high levels to induce QS. Presumably under conditions with restricted d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dulla G,Lindow SE

更新日期：2008-02-26 00:00:00

abstract：:Ribonucleotide reductase (RNR) catalyzes the conversion of nucleoside diphosphates to deoxynucleoside diphosphates (dNDPs). The Escherichia coli class Ia RNR uses a mechanism of radical propagation by which a cysteine in the active site of the RNR large (α2) subunit is transiently oxidized by a stable tyrosyl radical ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Minnihan EC,Ando N,Brignole EJ,Olshansky L,Chittuluru J,Asturias FJ,Drennan CL,Nocera DG,Stubbe J

更新日期：2013-03-05 00:00:00

abstract：:There is a high mortality in patients with diabetes and severe pressure ulcers. For example, chronic pressure sores of the heels often lead to limb loss in diabetic patients. A major factor underlying this is reduced neovascularization caused by impaired activity of the transcription factor hypoxia inducible factor-1 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duscher D,Neofytou E,Wong VW,Maan ZN,Rennert RC,Inayathullah M,Januszyk M,Rodrigues M,Malkovskiy AV,Whitmore AJ,Walmsley GG,Galvez MG,Whittam AJ,Brownlee M,Rajadas J,Gurtner GC

更新日期：2015-01-06 00:00:00

abstract：:Thermotaxis by individual amoebae of Dictyostelium discoideum on a temperature gradient is described. These amoebae show positive thermotaxis at temperatures between 14 degrees C and 28 degrees C shortly (3 hr) after food depletion. Increasing time on the gradient reduces the positive thermotactic response at the lowe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong CB,Fontana DR,Poff KL

更新日期：1983-09-01 00:00:00

abstract：:The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Supatto W,Débarre D,Moulia B,Brouzés E,Martin JL,Farge E,Beaurepaire E

更新日期：2005-01-25 00:00:00

abstract：:Among the most striking aspects of the movement of many animal groups are their sudden coherent changes in direction. Recent observations of locusts and starlings have shown that this directional switching is an intrinsic property of their motion. Similar direction switches are seen in self-propelled particle and othe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yates CA,Erban R,Escudero C,Couzin ID,Buhl J,Kevrekidis IG,Maini PK,Sumpter DJ

更新日期：2009-04-07 00:00:00

abstract：:The positive transcription elongation factor b (P-TEFb) is involved in physiological and pathological events including inflammation, cancer, AIDS, and cardiac hypertrophy. The balance between its active and inactive form is tightly controlled to ensure cellular integrity. We report that the transcriptional repressor C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cherrier T,Le Douce V,Eilebrecht S,Riclet R,Marban C,Dequiedt F,Goumon Y,Paillart JC,Mericskay M,Parlakian A,Bausero P,Abbas W,Herbein G,Kurdistani SK,Grana X,Van Driessche B,Schwartz C,Candolfi E,Benecke AG,Van Lin

更新日期：2013-07-30 00:00:00

abstract：:Borrelia burgdorferi (Bb), the causative agent of Lyme disease, is transmitted to mammalian hosts through an arthropod (tick) vector. To establish infection, Bb must acquire essential nutrients, including transition metals, from its mammalian and tick hosts. Thus far, no metal transporter has been identified in Bb. He...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ouyang Z,He M,Oman T,Yang XF,Norgard MV

更新日期：2009-03-03 00:00:00

abstract：:It has been a widely accepted hypothesis that the molecular clock slows down during evolution of higher primates. By molecular cloning and nucleotide sequence comparison of a rhesus macaque alpha-globin gene to its homologs in man, orangutan, olive baboon, and other mammals, we demonstrate a burst of evolution of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaw JP,Marks J,Shen CC,Shen CK

更新日期：1989-02-01 00:00:00

abstract：:Hematopoietic stem/progenitor cells (HSPCs) possess the potentials of self-renewal, proliferation, and differentiation toward different lineages of blood cells. These cells not only play a primordial role in hematopoietic development but also have important clinical application. Characterization of the gene expression...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao M,Fu G,Wu JS,Zhang QH,Zhou J,Kan LX,Huang QH,He KL,Gu BW,Han ZG,Shen Y,Gu J,Yu YP,Xu SH,Wang YX,Chen SJ,Chen Z

更新日期：1998-07-07 00:00:00

abstract：:We have used expression cloning in Xenopus oocytes to isolate two different cDNAs encoding functional N-methyl-D-aspartate (NMDA) receptor subunits. The two receptors (NMDA-R1A and -R1B) display different pharmacologic properties as a consequence of alternative exon addition within the putative ligand-binding domain. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakanishi N,Axel R,Shneider NA

更新日期：1992-09-15 00:00:00

abstract：:Steep topography, a tropical climate, and mafic lithologies contribute to efficient chemical weathering and carbon sequestration in the Southeast Asian islands. Ongoing arc-continent collision between the Sunda-Banda arc system and Australia has increased the area of subaerially exposed land in the region since the mi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park Y,Maffre P,Goddéris Y,Macdonald FA,Anttila ESC,Swanson-Hysell NL

更新日期：2020-10-13 00:00:00

abstract：:Diisopropyl fluorophosphate (DFP) causes neurotoxicity related to an irreversible inhibition of acetylcholinesterase (AChE). Management of this intoxication includes: (i) pretreatment with reversible blockers of AChE, (ii) blockade of muscarinic receptors with atropine, and (iii) facilitation of GABA(A) receptor signa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pibiri F,Kozikowski AP,Pinna G,Auta J,Kadriu B,Costa E,Guidotti A

更新日期：2008-09-16 00:00:00

abstract：:The inward Na+ current underlying the action potential in nerve is terminated by inactivation. The preceding report shows that deletions within the intracellular linker between domains III and IV remove inactivation, but mutation of conserved basic and paired acidic amino acids has little effect. Here we show that sub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： West JW,Patton DE,Scheuer T,Wang Y,Goldin AL,Catterall WA

更新日期：1992-11-15 00:00:00