The combination of huperzine A and imidazenil is an effective strategy to prevent diisopropyl fluorophosphate toxicity in mice.

abstract：:The plant aquaporin plasma membrane intrinsic proteins (PIP) subfamily represents one of the main gateways for water exchange at the plasma membrane (PM). A fraction of this subfamily, known as PIP1, does not reach the PM unless they are coexpressed with a PIP2 aquaporin. Although ubiquitous and abundantly expressed, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yaneff A,Sigaut L,Marquez M,Alleva K,Pietrasanta LI,Amodeo G

更新日期：2014-01-07 00:00:00

abstract：:The neu gene is distantly related to the erbB gene and encodes a cell surface protein that appears to function as a growth factor receptor. To study the mechanisms that caused the conversion of the normal neu gene to an oncogenic allele, we have isolated molecular clones of the neu oncogene as well as a clone of the c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hung MC,Schechter AL,Chevray PY,Stern DF,Weinberg RA

更新日期：1986-01-01 00:00:00

abstract：:We have cloned four lambda light chain constant region (C) genes from mouse embryo DNA. Each carries its own joining (J) segment approximately 1.3 kilobases to its 5' side. The four C genes occur in two clusters, 5' J3C3J1C13' and 5' J2C2J4C43', with C4 being a new C lambda gene. We have also shown that V lambda 1 is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blomberg B,Traunecker A,Eisen H,Tonegawa S

更新日期：1981-06-01 00:00:00

abstract：:Specific rearrangement of internal chromosomal regions occurs during development of the somatic macronucleus in Tetrahymena thermophila and results in elimination of germ-line (micronuclear) DNA sequences. The timing and mechanism of genome rearrangement within one particular 9.3-kilobase region, which contains three ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Austerberry CF,Allis CD,Yao MC

更新日期：1984-12-01 00:00:00

abstract：:A 978-base-pair gene that encodes thymidylate synthase (TS; 5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.1.45) from Lactobacillus casei has been synthesized and inserted into Escherichia coli expression vectors. The DNA sequence contains 35 unique restriction sites that are located an average of 28 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Climie S,Santi DV

更新日期：1990-01-01 00:00:00

abstract：:We have devised a simple and efficient cDNA cloning strategy that overcomes many of the difficulties encountered in obtaining full-length cDNA clones of low-abundance mRNAs. In essence, cDNAs are generated by using the DNA polymerase chain reaction technique to amplify copies of the region between a single point in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frohman MA,Dush MK,Martin GR

更新日期：1988-12-01 00:00:00

abstract：:Mind wandering represents the human capacity for internally focused thought and relies upon the brain's default network and its interactions with attentional networks. Studies have characterized mind wandering in healthy people, yet there is limited understanding of how this capacity is affected in clinical population...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Callaghan C,Shine JM,Hodges JR,Andrews-Hanna JR,Irish M

更新日期：2019-02-19 00:00:00

abstract：:Mycobacterium marinum, a natural pathogen of fish and frogs and an occasional pathogen of humans, is capable of inducing actin tail formation within the cytoplasm of macrophages, leading to actin-based motility and intercellular spread. Actin tail formation by M. marinum is markedly reduced in macrophages deficient in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stamm LM,Pak MA,Morisaki JH,Snapper SB,Rottner K,Lommel S,Brown EJ

更新日期：2005-10-11 00:00:00

abstract：:X chromosome-linked severe combined immunodeficiency disease (SCIDX1) is characterized by the absence of T-cell and natural killer cell development and results from molecular mutations of the interleukin 2 receptor (IL-2R) gamma chain. The IL-2R gamma chain is a common component of the IL-2, IL-4, and IL-7 receptor sy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DiSanto JP,Rieux-Laucat F,Dautry-Varsat A,Fischer A,de Saint Basile G

更新日期：1994-09-27 00:00:00

abstract：:A central role for phenotypic plasticity in adaptive evolution is often posited yet lacks empirical support. Selection for the stable production of an induced phenotype is hypothesized to modify the regulation of preexisting developmental pathways, producing rapid adaptive change. We examined the role of plasticity in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scoville AG,Pfrender ME

更新日期：2010-03-02 00:00:00

abstract：:Although introduced hemosporidian (malaria) parasites (Apicomplexa: Haemosporida) have hastened the extinction of endemic bird species in the Hawaiian Islands and perhaps elsewhere, little is known about the temporal dynamics of endemic malaria parasite populations. Haemosporidian parasites do not leave informative fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soares L,Latta SC,Ricklefs RE

更新日期：2017-06-20 00:00:00

abstract：:Molecular chaperones are an essential part of the machinery that avoids protein aggregation and misfolding in vivo. However, understanding the molecular basis of how chaperones prevent such undesirable interactions requires the conformational changes within substrate proteins to be probed during chaperone action. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kellner R,Hofmann H,Barducci A,Wunderlich B,Nettels D,Schuler B

更新日期：2014-09-16 00:00:00

abstract：:Cellular communities in living tissues act in concert to establish intricate microenvironments, with complexity difficult to recapitulate in vitro. We report a method for docking numerous cellularized hydrogel shapes (100-1,000 µm in size) into hydrogel templates to construct 3D cellular microenvironments. Each shape ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eng G,Lee BW,Parsa H,Chin CD,Schneider J,Linkov G,Sia SK,Vunjak-Novakovic G

更新日期：2013-03-19 00:00:00

abstract：:A mutant (ML 308-22) was isolated from Escherichia coli ML 308, which had lost the normal capacity to accumulate lactose analogs despite an increase in the membrane carrier activity. The exit of thiomethylgalactoside was much faster than normal, accounting for the inability of the cell to maintain high intracellular c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong PT,Kashket ER,Wilson TH

更新日期：1970-01-01 00:00:00

abstract：:We explored the detection of genome-wide hypomethylation in plasma using shotgun massively parallel bisulfite sequencing as a marker for cancer. Tumor-associated copy number aberrations (CNAs) could also be observed from the bisulfite DNA sequencing data. Hypomethylation and CNAs were detected in the plasma DNA of pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan KC,Jiang P,Chan CW,Sun K,Wong J,Hui EP,Chan SL,Chan WC,Hui DS,Ng SS,Chan HL,Wong CS,Ma BB,Chan AT,Lai PB,Sun H,Chiu RW,Lo YM

更新日期：2013-11-19 00:00:00

abstract：:Targeted gene modification mediated by single-stranded oligonucleotides (SSOs) holds great potential for widespread use in a number of biological and biomedical fields, including functional genomics and gene therapy. By using this approach, specific genetic changes have been created in a number of prokaryotic and euka...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu XS,Xin L,Yin WX,Shang XY,Lu L,Watt RM,Cheah KS,Huang JD,Liu DP,Liang CC

更新日期：2005-02-15 00:00:00

abstract：:In the plant Nicotiana plumbaginifolia, manganese superoxide dismutase (MnSOD) is synthesized in the cytoplasm as a preprotein and is subsequently translocated to the mitochondrial matrix with corresponding cleavage of an NH2-terminal leader sequence. To determine whether the plant enzyme could replace the endogenous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowler C,Alliotte T,Van den Bulcke M,Bauw G,Vandekerckhove J,Van Montagu M,Inzé D

更新日期：1989-05-01 00:00:00

abstract：:The tat protein encoded by the human immunodeficiency virus type 1 is a potent trans-activator of gene expression from the viral long terminal repeat. The domains that are essential for trans-activation, a Pro-Xaa3-Pro triad, a cysteine-rich metal-binding sequence motif, and a cluster of basic residues, are present wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jeyapaul J,Reddy MR,Khan SA

更新日期：1990-09-01 00:00:00

abstract：:Zeolite and zeolite-like molecular sieves are being used in a large number of applications such as adsorption and catalysis. Achievement of the long-standing goal of creating a chiral, polycrystalline molecular sieve with bulk enantioenrichment would enable these materials to perform enantioselective functions. Here, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brand SK,Schmidt JE,Deem MW,Daeyaert F,Ma Y,Terasaki O,Orazov M,Davis ME

更新日期：2017-05-16 00:00:00

abstract：:Radiation dose rates were evaluated in three areas neighboring a restricted area within a 20- to 50-km radius of the Fukushima Daiichi Nuclear Power Plant in August-September 2012 and projected to 2022 and 2062. Study participants wore personal dosimeters measuring external dose equivalents, almost entirely from depos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harada KH,Niisoe T,Imanaka M,Takahashi T,Amako K,Fujii Y,Kanameishi M,Ohse K,Nakai Y,Nishikawa T,Saito Y,Sakamoto H,Ueyama K,Hisaki K,Ohara E,Inoue T,Yamamoto K,Matsuoka Y,Ohata H,Toshima K,Okada A,Sato H,Kuwa

更新日期：2014-03-11 00:00:00

abstract：:A species of Rhodopseudomonas that grows under strict anaerobic conditions in the dark and requires CO was isolated from lake and pond sediments. Although anaerobic growth in the dark occurs in a chemically defined mineral medium with CO as the only carbon and energy source, growth is stimulated by adding trypticase. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uffen RL

更新日期：1976-09-01 00:00:00

abstract：:Recent work has demonstrated that Web search volume can "predict the present," meaning that it can be used to accurately track outcomes such as unemployment levels, auto and home sales, and disease prevalence in near real time. Here we show that what consumers are searching for online can also predict their collective...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goel S,Hofman JM,Lahaie S,Pennock DM,Watts DJ

更新日期：2010-10-12 00:00:00

abstract：:We document male-biased sex ratios among U.S.-born children of Chinese, Korean, and Asian Indian parents in the 2000 U.S. Census. This male bias is particularly evident for third children: If there was no previous son, sons outnumbered daughters by 50%. By contrast, the sex ratios of eldest and younger children with a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Almond D,Edlund L

更新日期：2008-04-15 00:00:00

abstract：:Phycobilisomes isolated from eight different species of cyanobacteria contain in addition to the light-harvesting phycobiliproteins, a small number of colorless polypeptides with molecular weights higher than those of the chromopolypeptide subunits of the phycobiliproteins. In the phycobilisomes of the species examin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Marsac NT,Cohen-bazire G

更新日期：1977-04-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization (MALDI) time of flight mass spectrometry was used to detect and order DNA fragments generated by Sanger dideoxy cycle sequencing. This was accomplished by improving the sensitivity and resolution of the MALDI method using a delayed ion extraction technique (DE-MALDI). The cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roskey MT,Juhasz P,Smirnov IP,Takach EJ,Martin SA,Haff LA

更新日期：1996-05-14 00:00:00

abstract：:One signature of adaptive radiation is a high level of trait change early during the diversification process and a plateau toward the end of the radiation. Although the study of the tempo of evolution has historically been the domain of paleontologists, recently developed phylogenetic tools allow for the rigorous exam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agrawal AA,Fishbein M,Halitschke R,Hastings AP,Rabosky DL,Rasmann S

更新日期：2009-10-27 00:00:00

abstract：:The plant Mentzelia pumila (family Loasaceae) has leaves and stems densely covered with tiny hooked trichomes. The structures entrap and kill insects and therefore are most probably protective. But they are also maladaptive in that they incapacitate a coccinellid beetle (Hippodamia convergens) that preys upon an aphid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eisner T,Eisner M,Hoebeke ER

更新日期：1998-04-14 00:00:00

abstract：:We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alwine JC,Kemp DJ,Stark GR

更新日期：1977-12-01 00:00:00

abstract：:Ig class-switch recombination (CSR) is directed by the long and repetitive switch regions and requires activation-induced cytidine deaminase (AID). One of the conserved switch-region sequence motifs (AGCT) is a preferred site for AID-mediated DNA-cytosine deamination. By using somatic gene targeting and recombinase-me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han L,Masani S,Yu K

更新日期：2011-07-12 00:00:00

abstract：:The effects of urea and of guanidinium chloride on binding constants in water for 6-(4-tert-butylanilino)-naphthalene-2-sulfonate and of bis(p-tert-butylphenyl) phosphate binding to beta-cyclodextrin and to N,N'-bis(6-beta-cyclo-dextrinyl)imidazolium ion have been determined. Their effects on the water solubility of p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breslow R,Halfon S

更新日期：1992-08-01 00:00:00