Abstract:
:The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.
journal_name
Proc Natl Acad Sci U S Aauthors
Supatto W,Débarre D,Moulia B,Brouzés E,Martin JL,Farge E,Beaurepaire Edoi
10.1073/pnas.0405316102keywords:
subject
Has Abstractpub_date
2005-01-25 00:00:00pages
1047-52issue
4eissn
0027-8424issn
1091-6490pii
0405316102journal_volume
102pub_type
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