Two sodium-channel genes in Drosophila: implications for channel diversity.

abstract：:The generation of high-titer, helper-free retroviruses by transient transfection has been achieved by using the highly transfectable 293T cell line into which are stably introduced constructs that express retroviral packaging functions. The resulting ecotropic virus packaging cell line BOSC 23 produces infectious retr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pear WS,Nolan GP,Scott ML,Baltimore D

更新日期：1993-09-15 00:00:00

abstract：:We describe a method that combines two- and three-color single-molecule FRET spectroscopy with 2D FRET efficiency-lifetime analysis to probe the oligomerization process of intrinsically disordered proteins. This method is applied to the oligomerization of the tetramerization domain (TD) of the tumor suppressor protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung HS,Meng F,Kim JY,McHale K,Gopich IV,Louis JM

更新日期：2017-08-15 00:00:00

abstract：:We describe here the successful establishment of a reverse genetics system for rotavirus (RV), a member of the Reoviridae family whose genome consists of 10-12 segmented dsRNA. The system is based on the recombinant vaccinia virus T7 RNA polymerase-driven procedure for supplying artificial viral mRNA in the cytoplasm....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Komoto S,Sasaki J,Taniguchi K

更新日期：2006-03-21 00:00:00

abstract：:Ascorbic acid metabolism is associated with a number of mechanisms known to be involved in host resistance to malignant disease. Cancer patients are significantly depleted of ascorbic acid, and in our opinion this demonstrable biochemical characteristic indicates a substantially increased requirement and utilization o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cameron E,Pauling L

更新日期：1976-10-01 00:00:00

abstract：:Prions are thought to consist mainly or entirely of misfolded PrP, a constitutively expressed host protein. Prions associated with the same PrP sequence may occur in the form of different strains; the strain phenotype is believed to be encoded by the conformation of the PrP. Some cell lines can be persistently infecte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahal SP,Baker CA,Demczyk CA,Smith EW,Julius C,Weissmann C

更新日期：2007-12-26 00:00:00

abstract：:IL-33, a cytokine of the IL-1 family, is closely associated with type II T cell responses. Here, we report an unexpected proinflammatory role of IL-33 in inflammatory arthritis. IL-33 was expressed in synovial fibroblasts from patients with rheumatoid arthritis (RA). Expression was markedly elevated in vitro by inflam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu D,Jiang HR,Kewin P,Li Y,Mu R,Fraser AR,Pitman N,Kurowska-Stolarska M,McKenzie AN,McInnes IB,Liew FY

更新日期：2008-08-05 00:00:00

abstract：:To generate antibodies with different effector functions, B cells undergo Immunoglobulin Heavy Chain (IgH) class switch recombination (CSR). The ligation step of CSR is usually mediated by the classical nonhomologous end-joining (cNHEJ) pathway. In cNHEJ-deficient cells, a remarkable ∼25% of CSR can be achieved by the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang XS,Zhao J,Wu-Baer F,Shao Z,Lee BJ,Cupo OM,Rabadan R,Gautier J,Baer R,Zha S

更新日期：2020-10-13 00:00:00

abstract：:The very low density lipoprotein (VLDL) receptor is a recently cloned member of the low density lipoprotein (LDL) receptor family that mediates the binding and uptake of VLDL when overexpressed in animal cells. Its sequence is 94% identical in humans and rabbits and 84% identical in humans and chickens, implying a con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frykman PK,Brown MS,Yamamoto T,Goldstein JL,Herz J

更新日期：1995-08-29 00:00:00

abstract：:Melanopsin, the photopigment of the "circadian" receptors that regulate the biological clock and the pupillary reflex in mammals, is homologous to invertebrate rhodopsins. Evidence supporting the involvement of phosphoinositides in light-signaling has been garnered, but the downstream effectors that control the light-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peinado G,Osorno T,Gomez Mdel P,Nasi E

更新日期：2015-06-23 00:00:00

abstract：:A 978-base-pair gene that encodes thymidylate synthase (TS; 5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.1.45) from Lactobacillus casei has been synthesized and inserted into Escherichia coli expression vectors. The DNA sequence contains 35 unique restriction sites that are located an average of 28 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Climie S,Santi DV

更新日期：1990-01-01 00:00:00

abstract：:Networks are widely used in the biological, physical, and social sciences as a concise mathematical representation of the topology of systems of interacting components. Understanding the structure of these networks is one of the outstanding challenges in the study of complex systems. Here we describe a general techniq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newman ME,Leicht EA

更新日期：2007-06-05 00:00:00

abstract：:The thin layer of airway surface liquid (ASL) contains antimicrobial substances that kill the small numbers of bacteria that are constantly being deposited in the lungs. An increase in ASL salt concentration inhibits the activity of airway antimicrobial factors and may partially explain the pathogenesis of cystic fibr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zabner J,Seiler MP,Launspach JL,Karp PH,Kearney WR,Look DC,Smith JJ,Welsh MJ

更新日期：2000-10-10 00:00:00

abstract：:The Escherichia coli chaperonins GroEL and GroES facilitate the refolding of polypeptide chains in an ATP hydrolysis-dependent reaction. The elementary steps in the binding and release of polypeptide substrates to GroEL were investigated in surface plasmon resonance studies to measure the rates of binding and dissocia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin Z,Eisenstein E

更新日期：1996-03-05 00:00:00

abstract：:Deficiency of acyl CoA:cholesterol acyltransferase 2 (ACAT2) in mice results in a reduction in cholesterol ester synthesis in the small intestine and liver, which in turn limits intestinal cholesterol absorption, hepatic cholesterol gallstone formation, and the accumulation of cholesterol esters in the plasma lipoprot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willner EL,Tow B,Buhman KK,Wilson M,Sanan DA,Rudel LL,Farese RV Jr

更新日期：2003-02-04 00:00:00

abstract：:The product of the recA gene of E. coli has been identified by labeling proteins synthesized in UV-treated cells after infection with specialized transducing phages carrying the recA gene. Following infection of UV-treated cells by lambda precA, which carries the recA+ gene, a major protein with a molecular weight of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McEntee K,Hesse JE,Epstein W

更新日期：1976-11-01 00:00:00

abstract：:Understanding how institutional changes within academia may affect the overall potential of science requires a better quantitative representation of how careers evolve over time. Because knowledge spillovers, cumulative advantage, competition, and collaboration are distinctive features of the academic profession, both...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petersen AM,Riccaboni M,Stanley HE,Pammolli F

更新日期：2012-04-03 00:00:00

abstract：:Clostridium perfringens iota-toxin (Ia) mono-ADP ribosylates Arg177 of actin, leading to cytoskeletal disorganization and cell death. To fully understand the reaction mechanism of arginine-specific mono-ADP ribosyl transferase, the structure of the toxin-substrate protein complex must be characterized. Recently, we so...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsurumura T,Tsumori Y,Qiu H,Oda M,Sakurai J,Nagahama M,Tsuge H

更新日期：2013-03-12 00:00:00

abstract：:A series of pyrimidine nucleoside analogues containing [2',5'-bis-O-(tert-butyldimethylsilyl)-3'-spiro-5''-(4''-amino- 1'',2''-oxathiole-2'',2''-dioxide)]-beta-D-ribofuranose as the pentose were found to inhibit human immunodeficiency virus type 1 [HIV-1(IIIB)] replication at a concentration of 0.06-0.8 microM but wer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balzarini J,Pérez-Pérez MJ,San-Félix A,Schols D,Perno CF,Vandamme AM,Camarasa MJ,De Clercq E

更新日期：1992-05-15 00:00:00

abstract：:Within the basal ganglia, acetylcholine and dopamine play a central role in the extrapyramidal control of motor function. The physiologic effects of these neurotransmitters are mediated by a diversity of receptor subtypes, several of which have now been cloned. Muscarinic acetylcholine receptors are encoded by five ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiner DM,Levey AI,Brann MR

更新日期：1990-09-01 00:00:00

abstract：:The x-ray crystal structures of the sulfide oxidase antibody 28B4 and of antibody 28B4 complexed with hapten have been solved at 2.2-angstrom and 1.9-angstrom resolution, respectively. To our knowledge, these structures are the highest resolution catalytic antibody structures to date and provide insight into the molec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsieh-Wilson LC,Schultz PG,Stevens RC

更新日期：1996-05-28 00:00:00

abstract：:Protein homeostasis is critically important for cell viability. Key to this process is the refolding of misfolded or aggregated proteins by molecular chaperones or, alternatively, their degradation by proteases. In most prokaryotes and in chloroplasts and mitochondria, protein degradation is performed by the caseinoly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vahidi S,Ripstein ZA,Bonomi M,Yuwen T,Mabanglo MF,Juravsky JB,Rizzolo K,Velyvis A,Houry WA,Vendruscolo M,Rubinstein JL,Kay LE

更新日期：2018-07-10 00:00:00

abstract：:The principal sequence feature responsible for intrinsic DNA curvature is generally assumed to be runs of adenines. However, according to the wedge model of DNA curvature, each dinucleotide step is associated with a characteristic deflection of the local helix axis. Thus, an important test of a more general view of se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bolshoy A,McNamara P,Harrington RE,Trifonov EN

更新日期：1991-03-15 00:00:00

abstract：:Synthesis of the 31-amino acid, inner membrane protein MgtS (formerly denoted YneM) is induced by very low Mg2+ in a PhoPQ-dependent manner in Escherichia coli Here we report that MgtS acts to increase intracellular Mg2+ levels and maintain cell integrity upon Mg2+ depletion. Upon development of a functional tagged de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang H,Yin X,Wu Orr M,Dambach M,Curtis R,Storz G

更新日期：2017-05-30 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are involved in a wide range of regulatory processes in the cell. Owing to their flexibility, their conformations are expected to be particularly sensitive to the crowded cellular environment. Here we use single-molecule Förster resonance energy transfer to quantify the effect ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soranno A,Koenig I,Borgia MB,Hofmann H,Zosel F,Nettels D,Schuler B

更新日期：2014-04-01 00:00:00

abstract：:The activation of additional promoter sites by production of an alternative sigma subunit for RNA polymerase is a common strategy for the coordinate regulation of gene expression. Many alternative sigma factors control genes for specialized, and often narrowly distributed, functions. For example, most of the alternati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YF,Helmann JD

更新日期：1992-06-01 00:00:00

abstract：:The ability to modulate the collective properties of correlated electron systems at their interfaces and surfaces underpins the burgeoning field of "designer" quantum materials. Here, we show how an electronic reconstruction driven by surface polarity mediates a Stoner-like magnetic instability to itinerant ferromagne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mazzola F,Sunko V,Khim S,Rosner H,Kushwaha P,Clark OJ,Bawden L,Marković I,Kim TK,Hoesch M,Mackenzie AP,King PDC

更新日期：2018-12-18 00:00:00

abstract：:The ability of long-term cultured and monoclonal T cell populations to provide antigen-specific help was assessed in a system of Ir gene-controlled in vitro antibody responses to soluble antigens. T-cell colonies and monoclonal T-cell lines were generated which proliferated specifically in response to poly(LTyr,Glu)-p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hodes RJ,Kimoto M,Hathcock KS,Fathman CG,Singer A

更新日期：1981-10-01 00:00:00

abstract：:A pentapeptide open reading frame equipped with a canonical ribosome-binding site is present in the Escherichia coli 23S rRNA. Overexpression of 23S rRNA fragments containing the mini-gene renders cells resistant to the ribosome-inhibiting antibiotic erythromycin. Mutations that change either the initiator or stop cod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tenson T,DeBlasio A,Mankin A

更新日期：1996-05-28 00:00:00

abstract：:Pathway proteomics strategies measure protein expression changes in specific cellular processes that carry out related functions. Using targeted tandem mass tags-based sample multiplexing, hundreds of proteins can be quantified across 10 or more samples simultaneously. To facilitate these highly complex experiments, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Q,Xiao H,Jedrychowski MP,Schweppe DK,Navarrete-Perea J,Knott J,Rogers J,Chouchani ET,Gygi SP

更新日期：2020-05-05 00:00:00

abstract：:The nonneural cholinergic system of immune cells is pivotal for the maintenance of immunological homeostasis. Here we demonstrate the expression of choline acetyltransferase (ChAT) and cholinergic enzymes in murine natural killer (NK) cells. The capacity for acetylcholine synthesis by NK cells increased markedly under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang W,Li D,Han R,Zhang C,Jin WN,Wood K,Liu Q,Shi FD,Hao J

更新日期：2017-07-25 00:00:00