Abstract:
:Prions are thought to consist mainly or entirely of misfolded PrP, a constitutively expressed host protein. Prions associated with the same PrP sequence may occur in the form of different strains; the strain phenotype is believed to be encoded by the conformation of the PrP. Some cell lines can be persistently infected by prions and, interestingly, show preference for certain strains. We report that a cloned murine neuroblastoma cell population, N2a-PK1, is highly heterogeneous in regard to its susceptibility to RML and 22L prions. Remarkably, sibling subclones may show very different relative susceptibilities to the two strains, indicating that the responses can vary independently. We have assembled four cell lines, N2a-PK1, N2a-R33, LD9 and CAD5, which show widely different responses to prion strains RML, 22L, 301C, and Me7, into a panel that allows their discrimination in vitro within 2 weeks, using the standard scrapie cell assay (SSCA).
journal_name
Proc Natl Acad Sci U S Aauthors
Mahal SP,Baker CA,Demczyk CA,Smith EW,Julius C,Weissmann Cdoi
10.1073/pnas.0710054104subject
Has Abstractpub_date
2007-12-26 00:00:00pages
20908-13issue
52eissn
0027-8424issn
1091-6490pii
0710054104journal_volume
104pub_type
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