当前位置： SCI文献检索 > GENERAL AND COMPARATIVE ENDOCRINOLOGY期刊下所有文献 > Cloning of the gene and complete cDNA encoding a type 2 deiodinase from Fundulus heteroclitus.

Cloning of the gene and complete cDNA encoding a type 2 deiodinase from Fundulus heteroclitus.

Abstract：

:Recently, we reported the cloning of a cDNA fragment from Fundulus heteroclitus liver encoding the open reading frame of type 2 deiodinase (FhD2). We here report the cloning of 14 kb of genomic sequence from F. heteroclitus that includes the previously reported coding region of the F. heteroclitus Dio2 gene (FhDio2), the 5(') and 3(') untranslated regions, and flanking regions and introns. This FhDio2 gene comprises two exons divided by a 4.8-kb intron. The position of the intron is similar to that of introns in other Dio2 genes. The analysis of approximately 1.3 kb of genomic sequence upstream of the mRNA start site revealed that, in contrast to mammalian Dio2 genes, there were no apparent TATA or CRE sequences. Nevertheless, a putative Sp1 site was found, similar to that in other F. heteroclitus TATA-less promoters. We have also cloned the complete FhD2 cDNA, which spans 4652 bp and contains a sequence adjacent to its poly(A) tail that is highly similar to the selenocysteine insertion sequence (SECIS) found in human D2 cDNA. The expression of a construct containing the FhD2 ORF plus the native SECIS resulted in a protein with deiodinase activity similar to that of the native FhD2. Analysis of the regulation of this gene, combined with ongoing studies of the F. heteroclitus D1 gene, will allow us to elucidate the functions of the colocalized deiodinases in teleost liver.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

Orozco A,Jeziorski MC,Linser PJ,Greenberg RM,Valverde-R C

doi

10.1016/s0016-6480(02)00071-0

keywords:

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

162-7

issue

2

eissn

0016-6480

issn

1095-6840

pii

S0016648002000710

journal_volume

128

pub_type

杂志文章

相关文献

GENERAL AND COMPARATIVE ENDOCRINOLOGY文献大全
  • Effects of bovine TSH on the tissue thyroxine level and metamorphosis in prometamorphic flounder larvae.

    abstract：:Bovine thyroid-stimulating hormone (TSH) was microinjected (0.2 microliter/fish) into prometamorphic flounder larvae and the effects on metamorphosis as well as the tissue thyroxine (T4) and triiodothyronine (T3) levels were studied. After a single injection of TSH (5 mIU/g), the tissue T4 concentration increased mark...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(89)80038-3

    authors： Inui Y,Tagawa M,Miwa S,Hirano T

    更新日期：1989-06-01 00:00:00

  • Cells in the intestinal system of holothurians (Echinodermata) express cholecystokinin-like immunoreactivity.

    abstract：:The presence of cholecystokinin (CCK), originally isolated from porcine small intestine, has been reported in a diversity of invertebrates ranging, from cnidarians to protochordates, but so far, not in echinoderms. We have used immunohistochemical techniques to demonstrate the presence of cells expressing CCK-like imm...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(91)90026-3

    authors： García-Arrarás JE,Torres-Avillán I,Ortíz-Miranda S

    更新日期：1991-08-01 00:00:00

  • Annual plasma testosterone and thyroxine cycles in relation to hibernation in the edible dormouse Glis glis.

    abstract：:Twelve male edible dormice, captured in the autumn of 1979, were studied for 2 years under natural lighting in an animal enclosure with permanently open windows, at Montpellier, in Southern France (latitude 43 degrees 50' N). Ambient temperature fluctuated annually between +4 and 27 degrees, and the photoperiod betwee...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(83)90266-6

    authors： Jallageas M,Assenmacher I

    更新日期：1983-06-01 00:00:00

  • Physiological responses to photoperiod in three cardueline finch species.

    abstract：:Cardueline finches (canaries, goldfinches, and rosefinches, etc.) vary widely in the degree to which their natural reproductive schedules track seasonal changes in photoperiod. In this study, we tested for photo-induction of reproductive development and photorefractoriness in males of three cardueline finch species: p...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2004.02.014

    authors： Hahn TP,Pereyra ME,Sharbaugh SM,Bentley GE

    更新日期：2004-05-15 00:00:00

  • Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.).

    abstract：:European silver eels are thought to undergo sexual maturation during their oceanic reproductive migration from the European continent to their spawning area in the Sargasso Sea. Tracking data and various anatomical and physiological features suggest that silver eels migrate in deep sea, leading us to hypothesise that ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2007.01.009

    authors： Sébert ME,Amérand A,Vettier A,Weltzien FA,Pasqualini C,Sébert P,Dufour S

    更新日期：2007-08-01 00:00:00

  • Effects of eyestalk removal on cholesterol uptake and ecdysone secretion by crab (Cancer antennarius) Y-organs in vitro.

    abstract：:Y-Organs and control tissues from intact (intermolt) and 48-hr de-eyestalked Cancer antennarius donors were cultured for 12 and 24 hr in crustacean saline supplemented 10% with crab serum and containing [14C]cholesterol. Under these conditions, Y-organs took up significantly more [14C]cholesterol than ovary or muscle,...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(85)90215-1

    authors： Watson RD,Spaziani E

    更新日期：1985-03-01 00:00:00

  • Testicular cycles in three species of Korean frogs: Rana nigromaculata, Rana rugosa, and Rana dybowskii.

    abstract：:Seasonal changes in testicular structure and function in three species of Korean frogs (Rana nigromaculata, R. rugosa, and R. dybowskii) having different breeding seasons and habitats were examined throughout the year. R. nigromaculata live in rice fields and breed in early May. Their gonadosomatic index (GSI) and tes...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1998.7118

    authors： Ko SK,Kang HM,Im WB,Kwon HB

    更新日期：1998-09-01 00:00:00

  • Juvenile hormone prevents the onset of programmed cell death in the prothoracic glands of Manduca sexta.

    abstract：:During normal pupal-adult development, programmed cell death of the prothoracic gland of Manduca sexta proceeds via apoptosis. By employing the DNA laddering technique, the earliest sign of DNA fragmentation in the cells comprising the gland occurred on Day 5 of pupal-adult development and DNA fragmentation peaked 1 d...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1997.7022

    authors： Dai JD,Gilbert LI

    更新日期：1998-02-01 00:00:00

  • Identification of a proglucagon cDNA from Rana tigrina rugulosa that encodes two GLP-1s and that is alternatively spliced in a tissue-specific manner.

    abstract：:Glucagon plays a pivotal role in the regulation of metabolism. A glucagon receptor has been previously characterized in the frog, Rana tigrina rugulosa, and the frog and human glucagon receptors have been shown to possess similar binding affinities toward human glucagon. To study the structural evolution of glucagon p...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.2001.7697

    authors： Yeung CM,Chow BK

    更新日期：2001-11-01 00:00:00

  • Apposition of enkephalinergic axons with cGnRH I-containing perikarya in turkey hen brain.

    abstract：:Several lines of evidence support a role of endogenous opioids in the brain's regulation of gonadotropin secretion in birds and mammals, although the neuroanatomic basis of such regulation is not clear. We used double-label fluorescence immunohistochemistry employing polyclonal antisera raised in sheep against chicken...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(02)00029-1

    authors： Millam JR,Wang R,Craig-Veit CB,Siopes TD

    更新日期：2002-06-15 00:00:00

  • Diurnal changes in plasma arginine vasotocin and isotocin in rainbow trout adapted to fresh water and brackish Baltic water.

    abstract：:The plasma levels of arginine vasotocin (AVT) and isotocin (IT) in rainbow trout (Oncorhynchus mykiss) were studied to assess possible diurnal variations in neurohypophysial nonapeptides. Fish were kept under natural photoperiod and adapted to fresh and brackish Baltic water. Blood was sampled at 5:00, 11:00, 16:00, 2...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1996.0162

    authors： Kulczykowska E,Stolarski J

    更新日期：1996-11-01 00:00:00

  • Frog brain and liver show evolutionary conservation of tissue-specific differences among insulin receptors.

    abstract：:The insulin receptors of frog brain and liver show features typical of other insulin receptors with regard to affinity and specificity of binding to insulins and proinsulin, solubility in Triton X-100, binding to and elution from wheat germ agglutinin, and insulin-sensitive tyrosine kinase activity. Likewise, the brai...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(87)90026-8

    authors： Hart C,Shemer J,Penhos JC,Lesniak MA,Roth J,LeRoith D

    更新日期：1987-11-01 00:00:00

  • Aldosterone and the control of secretion by the nasal salt gland of the north African desert lizard, Uromastix acanthinurus.

    abstract：:The secretory response of the nasal salt gland of the north African agamid lizard Uromastix acanthinurus to injections of 1 M KCl and NaCl is correlated with changes in circulating levels of the corticosteroids aldosterone and corticosterone. KCl loading led to a progressive rise in plasma aldosterone levels, and NaCl...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(84)90186-2

    authors： Bradshaw SD,Lemire M,Vernet R,Grenot CJ

    更新日期：1984-05-01 00:00:00

  • The effect of ovariectomy on concentrations of plasma prolactin and LH and parental behavior in the domestic fowl.

    abstract：:The role of ovarian hormones in the expression of parental behavior and in the regulation of LH secretion was investigated in incubating commercial meat-type hens. After ovariectomy, incubating hens continued to incubate eggs normally and brooded day-old chicks given to replace eggs, in a manner similar to sham-ovarie...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1996.0013

    authors： Lea RW,Richard-Yris MA,Sharp PJ

    更新日期：1996-01-01 00:00:00

  • Peripheral injections of cholecystokinin, apelin, ghrelin and orexin in cavefish (Astyanax fasciatus mexicanus): effects on feeding and on the brain expression levels of tyrosine hydroxylase, mechanistic target of rapamycin and appetite-related hormones.

    abstract：:The effects of intraperitoneal injections of cholecystokinin (CCK), apelin, ghrelin, and orexin on food intake were examined in the blind cavefish Astyanax fasciatus mexicanus. CCK (50ng/g) induced a decrease in food intake whereas apelin (100ng/g), orexin (100ng/g), and ghrelin (100ng/g) induced an increase in food i...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2013.11.015

    authors： Penney CC,Volkoff H

    更新日期：2014-01-15 00:00:00

  • Effects of P-nitrophenol exposure on the testicular development and semen quality of roosters.

    abstract：:The widespread use of P-nitrophenol (PNP) as a raw material in pesticides, medicines and dyes has led to environmental pollution. PNP is a well-known endocrine disruptor in mammals and quails. This study investigated the effects of long-term PNP exposure on the testicular development and semen quality of roosters. Pub...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2020.113656

    authors： Ren S,Li Y,Li C

    更新日期：2021-01-15 00:00:00

  • Estradiol and testosterone modulate the tissue-specific expression of ghrelin, ghs-r, goat and nucb2 in goldfish.

    abstract：:Ghrelin, and nesfatin-1 (encoded by nucleobindin2/nucb2) are two metabolic peptides with multiple biological effects in vertebrates. While sex steroids are known to regulate endogenous ghrelin and NUCB2 in mammals, such actions by steroids in fish remain unknown. This study aimed to determine whether estradiol (E2) an...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2016.01.006

    authors： Bertucci JI,Blanco AM,Canosa LF,Unniappan S

    更新日期：2016-03-01 00:00:00

  • Regulation of gill cytosolic corticosteroid receptors in juvenile Atlantic salmon: interaction effects of growth hormone with prolactin and triiodothyronine.

    abstract：:The potential effects of growth hormone (GH), prolactin (Prl), and triiodothyronine (T3) on gill Na+,K+-ATPase activity and corticosteroid receptor (CR) concentration (Bmax) and dissociation constant (Kd) were examined in juvenile Atlantic salmon (Salmo salar). Compared to controls, fish injected with GH (ovine, 5.0 m...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1998.7172

    authors： Shrimpton JM,McCormick SD

    更新日期：1998-11-01 00:00:00

  • Receptors for vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide in turkey cerebral cortex: characterization by [125I]-VIP binding and effects on cyclic AMP synthesis.

    abstract：:Receptors for vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) in turkey cerebral cortex were characterized using two approaches: (1) in vitro radioreceptor binding of [125I]-VIP, and (2) effects of peptides from the PACAP/VIP/secretin family on cyclic AMP formation. T...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2004.03.007

    authors： Zawilska JB,Niewiadomski P,Nowak JZ

    更新日期：2004-06-01 00:00:00

  • Effects of corticosteroids and protein synthesis inhibitors on activities of oxidative enzymes in a bony fish, Anabas testudineus (Bloch).

    abstract：:The activities of oxidative enzymes of hepatic mitochondria of Anabas testudineus increased following exogenous administration of hydrocortisone and corticosterone. The inhibitors of protein synthesis prevented the hormone-mediated increase of hepatic enzymes, suggesting that corticosteroids regulate the oxidative met...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(90)90017-g

    authors： Ignatius J,Oommen OV

    更新日期：1990-05-01 00:00:00

  • Maternal androgen levels during pregnancy are associated with early-life growth in Geoffroy's marmosets, Callithrix geoffroyi.

    abstract：:Fetal development is a critical period of physical development, and factors in the intrauterine environment can cause lasting effects on the growth and development of offspring. There is little research evaluating organizational effects of early androgen exposure of endogenous maternal origins on the prenatal and post...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2009.10.008

    authors： Smith AS,Birnie AK,French JA

    更新日期：2010-04-01 00:00:00

  • Developmental expression and subcellular localization of wallaby gonadotropin-releasing hormone receptor and its splice variants.

    abstract：:The developmental expression of gonadotropin-releasing hormone receptor (GnRH-R) and its splice variants was examined in the gonads of tammar wallaby pouch young in order to elucidate the functional role of GnRH-R in the developing testis and ovary. Wallaby GnRH-R, like eutherian GnRH-Rs, contains three exons and two ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(03)00146-1

    authors： Cheung TC,Hearn JP

    更新日期：2003-08-01 00:00:00

  • Effects of serotonin on gonadotropin and growth hormone release from in vitro perifused goldfish pituitary fragments.

    abstract：:The effects of serotonin (5-HT) on gonadotropin and growth hormone release from perifused goldfish (Carassius auratus, L.) pituitary glands were studied. Serotonin, at micromolar concentrations, caused a dose-related release of gonadotropin and an inhibition of growth hormone release in pituitaries from goldfish at di...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(91)90301-l

    authors： Somoza GM,Peter RE

    更新日期：1991-04-01 00:00:00

  • Plasma aldosterone, angiotensin II, and arginine vasotocin concentrations in the toad, Bufo marinus, following osmotic treatments.

    abstract：:To clarify the physiological roles of the renin-angiotensin-aldosterone system (RAAS) and arginine vasotocin (AVT) on body fluid regulation in amphibians, we measured plasma concentrations of aldosterone (ALDO), angiotensin II (ANG II), and AVT after various osmotic challenges in the marine toad, Bufo marinus (Bufonid...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2004.10.005

    authors： Konno N,Hyodo S,Takei Y,Matsuda K,Uchiyama M

    更新日期：2005-01-15 00:00:00

  • Immunocytochemical localization, HPLC characterization, and seasonal dynamics of vasotocin in the brain, blood plasma and gonads of the catfish Heteropneustes fossilis.

    abstract：:Immunocytochemical distribution and dynamics of vasotocin (VT) were studied in the air-breathing catfish Heteropneustes fossilis in relation to the reproductive cycle. Vasotocin was localized in the brain and ovary by streptavidin-biotin immunocytochemistry. The immunoreactivity was found throughout the hypothalamo-hy...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2008.09.003

    authors： Singh V,Joy KP

    更新日期：2008-11-01 00:00:00

  • Sequence analysis, endocrine regulation, and signal transduction of GnRH receptors in teleost fish.

    abstract：:Three gonadotropin-releasing hormones (GnRHs) and three cognate receptors have been identified in vertebrates, with distinct distributions and functions. According to their sequences, the receptors can be grouped into distinct classes: types I, II, and III. One branch contains all type-I GnRH receptors (GnRH-R-I) from...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygcen.2005.01.019

    authors： Levavi-Sivan B,Avitan A

    更新日期：2005-05-15 00:00:00

  • Purification of maturational gonadotropin from Atlantic croaker (Micropogonias undulatus) and development of a homologous radioimmunoassay.

    abstract：:The isolation of a maturational gonadotropin from pituitaries of Atlantic croaker (Micropogonias undulatus), a marine teleost belonging to the order perciformes, is described. A radioimmunoassay has been developed for the measurement of this hormone in blood samples and validation of the assay is presented. The hormon...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(89)90200-1

    authors： Copeland PA,Thomas P

    更新日期：1989-03-01 00:00:00

  • Modulation of metabolic and clock gene mRNA rhythms by pineal and retinal circadian oscillators.

    abstract：:Avian circadian organization involves interactions between three neural pacemakers: the suprachiasmatic nuclei (SCN), pineal, and retina. Each of these structures is linked within a neuroendocrine loop to influence downstream processes and peripheral oscillations. However, the contribution of each structure to drive o...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2008.12.015

    authors： Karaganis SP,Bartell PA,Shende VR,Moore AF,Cassone VM

    更新日期：2009-04-01 00:00:00

  • Growth hormone (GH)-releasing activity of chicken GH-releasing hormone (GHRH) in chickens.

    abstract：:Two peptides with sequence similarities to growth hormone releasing hormone (GHRH) have been identified by analysis of the chicken genome. One of these peptides, chicken (c) GHRH-LP (like peptide) was previously found to poorly bind to chicken pituitary membranes or to cloned and expressed chicken GHRH receptors and h...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2014.06.007

    authors： Harvey S,Gineste C,Gaylinn BD

    更新日期：2014-08-01 00:00:00

  • Isolation, synthesis, and biological activity of flounder [Asn1,Ile5,Thr9] angiotensin I.

    abstract：:A novel angiotensin I (ANG I) has been isolated from incubates of plasma and kidney extracts of the flounder, Platichthys flesus, using ion-exchange, gel-permeation, and reverse-phase high performance liquid chromatography (HPLC). Its sequence was determined as H-Asn-Arg-Val-Tyr-Ile-His-Pro-Phe-Thr-Leu-OH by sequence ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(02)00534-8

    authors： Balment RJ,Warne JM,Takei Y

    更新日期：2003-01-01 00:00:00