Abstract：
:During normal pupal-adult development, programmed cell death of the prothoracic gland of Manduca sexta proceeds via apoptosis. By employing the DNA laddering technique, the earliest sign of DNA fragmentation in the cells comprising the gland occurred on Day 5 of pupal-adult development and DNA fragmentation peaked 1 day later. Since juvenile hormone (JH) is known to prevent adult development and since prothoracic gland degeneration occurs during the initial stages of adult development, we wished to determine the possible role of JH in prothoracic gland maintenance. JH was injected into pupae and the glands analyzed by DNA laddering and in situ TUNEL labeling. The administration of JH prevented apoptosis of the prothoracic gland even 11 days after JH injection into young pupae. The prothoracic glands remained intact and their ability to synthesize ecdysteroids was maintained at a fairly active level as ascertained by radioimmunoassay after in vitro incubation. The control glands had degenerated by this time and were almost devoid of ecdysteroidogenesis capability. Ultrastructural analysis confirmed that JH-treated prothoracic gland cells were rescued from the apoptotic sequence, i.e., nuclear condensation, cytoplasmic budding, and cell fragmentation. They exhibited a preserved smooth endoplasmic reticulum and intercellular channel system typical of active prothoracic gland cells. The composite data suggest that JH can both maintain and stimulate the prothoracic gland. Therefore, during normal pupal-adult metamorphosis the absence of JH is prerequisite for both the initiation and completion of prothoracic gland degeneration.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Dai JD,Gilbert LIdoi
10.1006/gcen.1997.7022subject
Has Abstractpub_date
1998-02-01 00:00:00pages
155-65issue
2eissn
0016-6480issn
1095-6840pii
S0016-6480(97)97022-2journal_volume
109pub_type
杂志文章abstract：:We used a free-ranging, seasonally breeding adult male songbird, the rufous-winged sparrow, Aimophila carpalis, to investigate the effects of acute stress-induced by capture followed by restraint, on the hypothalamo-pituitary-testicular axis. Intra- and interindividual comparisons revealed that males decreased their p...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.07.009
更新日期：2010-10-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7326
更新日期：1999-09-01 00:00:00
abstract：:Fecal glucocorticoid metabolite analysis is a useful tool for monitoring adrenocortical activity in captive and free-ranging wildlife. Glucocorticoid metabolites may not be evenly distributed within fecal samples and this variability could affect the interpretation of glucocorticoid metabolite measurements. Furthermor...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00061-3
更新日期：2003-06-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7649
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abstract：:The role of stress in the decline of Steller sea lions has become of interest in the identification of factors leading to their drastic decline and subsequent failure to recover. Serum cortisol was validated as a potential indicator of stress in three sub-populations inhabiting the majority of the Steller sea lions ra...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.06.006
更新日期：2010-01-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1164
更新日期：1995-12-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.04.010
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.12.005
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90062-x
更新日期：1983-09-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.12.024
更新日期：2012-03-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90202-w
更新日期：1990-01-01 00:00:00
abstract：:The effect of salmon calcitonin (0.25 MRC mU/g body wt) was investigated on the serum calcium and inorganic phosphate levels of the frog. Rana tigrina. The hormone evokes hypocalcemia (on Day 1 and Day 3) which is followed by a significant hypercalcemia on Day 10. Thereafter, the level of calcium decreases again on Da...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90109-3
更新日期：1989-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.0172
更新日期：1996-12-01 00:00:00
abstract：:Prostaglandin F2 alpha (PGF2 alpha), progesterone, androgens (testosterone + dihydrotestosterone), and 17 beta-estradiol were measured in the plasma of male frogs, Rana esculenta, by radioimmunoassays. Plasma concentrations of PGF2 alpha were higher from October to December and peaked in March (prereproduction) and in...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90307-r
更新日期：1991-06-01 00:00:00
abstract：:Due to global and local climate changes, farmed salmon may experience periods of elevated sea temperatures. An experiment was conducted to examine endocrine and dietary effects of high sea temperatures in adult (2.0 kg) and sexually immature Atlantic salmon, Salmo salar L. Groups of salmon were exposed to 19 °C while ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.10.007
更新日期：2012-01-01 00:00:00
abstract：:The purpose of the present study was to determine if central administration of somatostatin influences feeding behavior in layer chicks. Five- to 7-day-old chicks that received intracerebroventricular (ICV) injections of 0.5 or 2 nmol somatostatin increased their food intake at 30 and 60 min after the injection, sugge...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2009-05-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.07.021
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journal_title：General and comparative endocrinology
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更新日期：2019-01-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7561
更新日期：2001-01-01 00:00:00
abstract：:The ontogeny of gonadotropin 1 (GtH1) and 2 (GtH2) cells and its possible link to gonadal sex differentiation were studied in pejerrey, Odontesthes bonariensis, by immunocytochemistry using anti-chum salmon beta-GtH1 and beta-GtH2 antisera. In adults, GtH1 cells were found in the proximalis pars distalls (PPD) close t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7687
更新日期：2001-10-01 00:00:00
abstract：:The aim of this work was to study, in the Cebus apella monkey, the developmental changes in the microanatomy of the utero-ovarian ligament (UOL) and whether their vascular and neural elements might be involved in the transfer of signals between the ovaries and uterus. Sections including uterus, UOL, and ovary obtained...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.06.006
更新日期：2005-12-01 00:00:00
abstract：:Gonadotropin-releasing hormone (GnRH) was detected in the brains of passerine birds, a recently evolved and diverse avian group. The molecular forms of GnRH in two species of birds under breeding conditions were deduced using methods of HPLC and immunology. The brain extracts of song sparrows (Melospiza melodia) conta...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1988-03-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90321-v
更新日期：1991-06-01 00:00:00
abstract：:Genetic sex determination leads to gonadal differentiation and ultimately the differences between the sexes in steroid hormone secretion. Gonadal steroidogenesis is critical for the development of a sexually dimorphic phenotype and adult reproductive function. Control of gonadal development and steroidogenesis is unde...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2002-06-01 00:00:00
abstract：:Stress-induced inhibition of innate immune activity has been observed in a variety of wild birds and may increase chances of infection because this activity constitutes the first line of defense against pathogens. We previously reported that the transient elevation of plasma corticosterone (CORT; the primary avian glu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2019.02.002
更新日期：2019-05-01 00:00:00
abstract：:Retinoid X receptors (RXRs) are members of the nuclear receptor superfamily and mediate development, reproduction, homeostasis and cell differentiation processes in vertebrates. In this study, full-length cDNA sequences of five rxr subtypes from yellow catfish Pelteobagrus fulvidraco were cloned. Their mRNA expression...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.10.010
更新日期：2016-01-01 00:00:00
abstract：:The vasoconstrictive and cardiovascular actions of a recently identified bradykinin (BK)-related peptide (Gly-Ile-Thr-Ser-Trp-Leu-Pro-Phe) from the little skate, Leucoraja erinacea were examined in the unanesthetised little skate. Intra-arterial administration of a skate BK (0.1-1 nmolkg(-1)) produced a hypertensive r...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.08.002
更新日期：2011-11-01 00:00:00
abstract：:We investigated the effect of several potential carbohydrate secretagogues, amino acids, a ketoacid, and potassium chloride on insulin, glucagon, and somatostatin release from the in vitro perfused Brockmann body of channel catfish (Ictalurus punctatus). Mannose (15 mM) stimulated the release of insulin and somatostat...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90120-1
更新日期：1987-03-01 00:00:00
